Analysis of an activated ABI5 allele using a new selection method for transgenic Arabidopsis seeds

The Arabidopsis abscisic acid (ABA) insensitive (ABI)5 transcription factor participates in the ABA‐dependent induction of late embryogenesis abundant (LEA) genes in the final stages of seed development. We tested whether the VP16 transcriptional activation domain is sufficient to provide ABI5 with the ability to activate the AtEm LEA genes in vegetative tissues. We took advantage of a new transgenic seed selection assay based on green fluorescent protein (GFP) fluorescence and found that VP16‐ABI5 triggered growth retardation and ABA‐independent induction of AtEm1 in seedlings. These results indicate that ABI5 activation potential is a limiting step and might be a target for ABA signaling.

• Publication year

  2004

• Venue

  FEBS Letters

• Publication date

  2004-03-12

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0014-5793(04)00148-6PMID 15013763
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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