Morphogen gradient reconstitution reveals Hedgehog pathway design principles

No abstract is available for this paper.

• Publication year

  2018

• Venue

  Science

• Publication date

  2018-05-04

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1126/science.aao0645PMID 29622726PMCID PMC6516753
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Buffering Global Variability of Morphogen Gradients.

  2017cited by this paper
• An operational view of intercellular signaling pathways

  2017cited by this paper
• Dynamics of epigenetic regulation at the single-cell level

  2016cited by this paper
• Scaling Pattern to Variations in Size during Development of the Vertebrate Neural Tube

  2016cited by this paper
• The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2016 update

  2016cited by this paper
• Genome engineering using CRISPR-Cas9 system.

  2015cited by this paper
• Ptch1 and Gli regulate Shh signalling dynamics via multiple mechanisms

  2015cited by this paper
• Morphogen rules: design principles of gradient-mediated embryo patterning

  2015cited by this paper
• A predictive model of bifunctional transcription factor signaling during embryonic tissue patterning.

  2014cited by this paper
• Positional Information, Positional Error, and Readout Precision in Morphogenesis: A Mathematical Framework

  2014cited by this paper
• The utility of paradoxical components in biological circuits.

  2013cited by this paper
• Multiplex Genome Engineering Using CRISPR/Cas Systems

  2013cited by this paper
• Genome engineering using the CRISPR-Cas9 system

  2013cited by this paper
• Cytonemes are required for the establishment of a normal Hedgehog morphogen gradient in Drosophila epithelia

  2013cited by this paper
• The mechanisms of Hedgehog signalling and its roles in development and disease

  2013cited by this paper
• An inducible transgene expression system for zebrafish and chick

  2013cited by this paper
• Specialized filopodia direct long-range transport of Shh during vertebrate tissue patterning

  2013cited by this paper
• Differential Diffusivity of Nodal and Lefty Underlies a Reaction-Diffusion Patterning System

  2012cited by this paper
• Pulsed Feedback Defers Cellular Differentiation

  2012cited by this paper
• Supplementary Materials for Differential Diffusivity of Nodal and Lefty Underlies a Reaction-Diffusion Patterning System

  2012cited by this paper
• Signaling mechanisms controlling cell fate and embryonic patterning.

  2012cited by this paper
• Gene Regulatory Logic for Reading the Sonic Hedgehog Signaling Gradient in the Vertebrate Neural Tube

  2012cited by this paper
• Morphogen gradients: from generation to interpretation.

  2011cited by this paper
• Cis Interactions between Notch and Delta Generate Mutually Exclusive Signaling States

  2010cited by this paper
• The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.

  2010cited by this paper
• Improved Tet-responsive promoters with minimized background expression

  2010cited by this paper
• TopHat: discovering splice junctions with RNA-Seq

  2009cited by this paper
• Morphogen gradient formation.

  2009cited by this paper
• Positive feedback in cellular control systems

  2008cited by this paper
• Stability and nuclear dynamics of the bicoid morphogen gradient.

  2007cited by this paper
• Kinetics of Morphogen Gradient Formation

  2007cited by this paper
• Signal dynamics in Sonic hedgehog tissue patterning

  2006cited by this paper
• Optimization of the Tet-On system for regulated gene expression through viral evolution

  2006cited by this paper
• The C-terminal tail of the Hedgehog receptor Patched regulates both localization and turnover.

  2006cited by this paper
• Reading the Hedgehog morphogen gradient by measuring the ratio of bound to unbound Patched protein

  2004cited by this paper
• Growth and pattern of the mammalian neural tube are governed by partially overlapping feedback activities of the hedgehog antagonists patched 1 and Hhip1

  2004cited by this paper
• The sonic hedgehog signaling system as a bistable genetic switch.

  2004cited by this paper
• Self-enhanced ligand degradation underlies robustness of morphogen gradients.

  2003cited by this paper
• Do morphogen gradients arise by diffusion?

  2002cited by this paper
• Patched acts catalytically to suppress the activity of Smoothened

  2002cited by this paper
• Negative autoregulation speeds the response times of transcription networks.

  2002cited by this paper
• A freely diffusible form of Sonic hedgehog mediates long-range signalling

  2001cited by this paper
• A hedgehog-insensitive form of patched provides evidence for direct long-range morphogen activity of sonic hedgehog in the neural tube.

  2001influential reference
• On the range of hedgehog signaling.

  2000cited by this paper
• Feedback control of intercellular signalling in development

  2000cited by this paper
• Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine

  2000cited by this paper
• Mouse patched1 controls body size determination and limb patterning.

  1999cited by this paper
• Negative feedback mechanisms and their roles during pattern formation.

  1999cited by this paper
• Vertebrate Hedgehog signalling modulated by induction of a Hedgehog-binding protein

  1999cited by this paper
• Dual roles for patched in sequestering and transducing Hedgehog.

  1996cited by this paper
• Biochemical evidence that Patched is the Hedgehog receptor

  1996cited by this paper
• Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by Hedgehog.

  1996cited by this paper
• Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function

  1996cited by this paper

• Formation control of swarm robotics: A survey from biological inspirations to design automation methods

  2026cites this paper
• Mechanistic tradeoffs between local and long-range signaling activity in natural and synthetic morphogens

  2026cites this paper
• Comparison between the activities of canonical Wnt ligands in human pluripotent stem cell differentiation

  2025cites this paper
• Exosome secretion is required for sonic hedgehog dispersal and signal gradient formation in the embryonic limb mesenchyme

  2025cites this paper
• Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model

  2025cites this paper
• Information bounds the robustness of self-organized systems

  2025cites this paper
• Turing-Type Catalytic 2D-Metal Nanomesh Inside Silica-Bilayer for Multicomponent Reaction Control.

  2025cites this paper
• Investigating morphogen and patterning dynamics with optogenetic control of morphogen production.

  2025cites this paper
• Midline assembloids reveal regulators of human axon guidance.

  2025cites this paper
• Engineering Development: From the Repressilator and Toggle Switch to Synthetic Developmental Biology.

  2025cites this paper
• Building RNA concentration fields

  2025cites this paper
• A systems view of cellular heterogeneity: Unlocking the "wheel of fate".

  2025cites this paper
• Challenges and Opportunities in Spatiotemporal Models of Mammalian Gastrulation.

  2025cites this paper
• How memory and adaptation cost shape cell phenotypic dynamics in response to fluctuating environments

  2025cites this paper
• Induced Neural Progenitor Specification from Human Pluripotent Stem Cells by a Refined Synthetic Notch Platform

  2025cites this paper
• Human Synthetic Biology and Programmable Gene Regulation Control.

  2025cites this paper
• Magnetized Cellbots to Spatiotemporally Control Differentiation of Human-Induced Pluripotent Stem Cells

  2025cites this paper
• Large-scale control over collective cell migration using light-activated epidermal growth factor receptors.

  2025cites this paper
• Live imaging of paracrine signaling: Advances in visualization and tracking techniques

  2025cites this paper
• Synthetic forms most beautiful: engineering insights into self-organization.

  2025cites this paper
• A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradients in vitro

  2025cites this paper
• The evolution of developmental biology through conceptual and technological revolutions.

  2024cites this paper
• Biofabrication of spatially organized temporo-mandibular fibrocartilage assembloids

  2024influential citation
• Control of spatio-temporal patterning via cell growth in a multicellular synthetic gene circuit

  2024cites this paper
• Dynamic readout of the Hh gradient in the Drosophila wing disc reveals pattern-specific tradeoffs between robustness and precision

  2024cites this paper
• Templated Pluripotent Stem Cell Differentiation via Substratum-Guided Artificial Signaling

  2024cites this paper
• Robust tissue pattern formation by coupling morphogen signal and cell adhesion

  2024cites this paper
• Morphogens in the evolution of size, shape and patterning

  2024cites this paper
• Synthetically programming natural cell-cell communication pathways for tissue engineering.

  2024cites this paper
• Diffusion barriers imposed by tissue topology shape Hedgehog morphogen gradients

  2024cites this paper
• Midline Assembloids Reveal Regulators of Human Axon Guidance

  2024cites this paper
• Illuminating morphogen and patterning dynamics with optogenetic control of morphogen production

  2024cites this paper
• Large-scale control over collective cell migration using light-controlled epidermal growth factor receptors

  2024cites this paper
• Mathematical and In Silico Analysis of Synthetic Inhibitory Circuits That Program Self-Organizing Multicellular Structures.

  2024cites this paper
• Diffusion barriers imposed by tissue topology shape morphogen gradients

  2024cites this paper
• Localized application of SAG21k-loaded fibrin hydrogels for targeted modulation of the hedgehog pathway in facial nerve injury.

  2024cites this paper
• Dynamics of morphogen source formation in a growing tissue

  2024cites this paper
• Mechanistic Model-Driven Biodesign in Mammalian Synthetic Biology.

  2024cites this paper
• Understanding and manipulating extracellular behaviors of Wnt ligands

  2024cites this paper
• Establishing Hedgehog Gradients during Neural Development

  2023cites this paper
• Tradeoff Between Speed and Robustness in Primordium Initiation Mediated by Auxin-CUC1 Interaction

  2023cites this paper
• Design and Mathematical Analysis of Synthetic Inhibitory Circuits that Program Self-Organizing Multicellular Structures

  2023cites this paper
• Spatiotemporal, optogenetic control of gene expression in organoids

  2023cites this paper
• Phenotypically Discordant Anomalies in Conjoined Twins: Quirks of Nature Governed by Molecular Pathways?

  2023cites this paper
• Engineering global and local signal generators for probing temporal and spatial cellular signaling dynamics

  2023cites this paper
• Quantifying Gene Expression Domains in Plant Shoot Apical Meristems.

  2023cites this paper
• Reconstitution of morphogen shuttling circuits

  2023cites this paper
• Control of Tissue Development by Morphogens.

  2023cites this paper
• The logistics of Wnt production and delivery.

  2023cites this paper
• Hedgehog is relayed through dynamic heparan sulfate interactions to shape its gradient

  2023cites this paper
• Coreceptor Functions of Cell Surface Heparan Sulfate Proteoglycans.

  2022cites this paper
• Synthetic Morphogenesis: Introducing IEEE Journal Readers to Programming Living Mammalian Cells to Make Structures

  2022cites this paper
• Regulation of FGF-2, FGF-18 and Transcription Factor Activity by Perlecan in the Maturational Development of Transitional Rudiment and Growth Plate Cartilages and in the Maintenance of Permanent Cartilage Homeostasis

  2022cites this paper
• Principles for the design of multicellular engineered living systems

  2022cites this paper
• Periodic spatial patterning with a single morphogen

  2022cites this paper
• Mapping and exploring the organoid state space using synthetic biology.

  2022cites this paper
• Advances and challenges in programming pattern formation using living cells.

  2022cites this paper
• Synthetic developmental biology: New tools to deconstruct and rebuild developmental systems.

  2022cites this paper
• Sonic Hedgehog Intron Variant Associated With an Unusual Pediatric Cortical Cataract

  2022cites this paper
• Creating artificial signaling gradients to spatially pattern engineered tissues.

  2022cites this paper
• Patterning principles of morphogen gradients

  2022cites this paper
• Logical design of synthetic cis-regulatory DNA for genetic tracing of cell identities and state changes

  2022cites this paper
• From mesenchymal niches to engineered in vitro model systems: Exploring and exploiting biomechanical regulation of vertebrate hedgehog signalling

  2022cites this paper
• Periodic inhibition of Erk activity drives sequential somite segmentation

  2022cites this paper
• Dynamic readout of the Hh gradient in the Drosophila wing disc reveals pattern-specific tradeoffs between robustness and precision

  2022cites this paper
• Nodal is a short-range morphogen with activity that spreads through a relay mechanism in human gastruloids

  2022cites this paper
• Bioprinting for the Biologist.

  2021cites this paper
• Robustness and evolvability in transcriptional regulation

  2021cites this paper
• Programmed Morphogenesis: Methods and Protocols

  2021cites this paper
• Global community effect: large-scale cooperation yields collective Global community effect: large-scale cooperation yields collective survival of differentiating embryonic stem cells [preprint] survival of differentiating embryonic stem cells [preprint] us know how access to this document benefits y

  2021cites this paper
• Spatio-temporal, optogenetic control of gene expression in organoids

  2021cites this paper
• Context-aware synthetic biology by controller design: Engineering the mammalian cell.

  2021cites this paper
• Novel synthetic biology approaches for developmental systems

  2021influential citation
• Phototriggered Spatially Controlled Out-of-Equilibrium Patterns of Peptide Nanofibers in a Self-Sorting Double Network Hydrogel.

  2021cites this paper
• Hedgehog signaling and the primary cilium: implications for spatial and temporal constraints on signaling.

  2021cites this paper
• Nodal is a short-range morphogen with activity that spreads through a relay mechanism in human gastruloids

  2021cites this paper
• Protease-controlled secretion and display of intercellular signals

  2021cites this paper
• Addressing evolutionary questions with synthetic biology

  2021cites this paper
• The Role of Sonic Hedgehog in Human Holoprosencephaly and Short-Rib Polydactyly Syndromes

  2021cites this paper
• Dispatching plasma membrane cholesterol and Sonic Hedgehog dispatch: two sides of the same coin?

  2021cites this paper
• Regulated delivery controls Drosophila Hedgehog, Wingless, and Decapentaplegic signaling

  2021cites this paper
• Generation of extracellular morphogen gradients: the case for diffusion

  2021cites this paper
• The effects of Hh morphogen source movement on signaling dynamics

  2021cites this paper
• Expanding the boundaries of synthetic development.

  2021cites this paper
• Cost-precision trade-off relation determines the optimal morphogen gradient for accurate biological pattern formation

  2021cites this paper
• Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis

  2021influential citation
• A Microphysiological Approach to Evaluate Effectors of Intercellular Hedgehog Signaling in Development

  2021cites this paper
• A Modular Computational Framework for the Design of Multicellular Genetic Circuits of 1 Morphogenesis 2

  2020cites this paper
• Light-Activated Signaling in DNA-Encoded Sender–Receiver Architectures

  2020cites this paper
• Cell patterning by surface tension pinning in microfluidic channels.

  2020cites this paper
• Tradeoff between speed and robustness in primordium initiation mediated by auxin-CUC1 interaction

  2020cites this paper
• Global community effect: large-scale cooperation yields collective survival of differentiating embryonic stem cells [preprint]

  2020cites this paper
• Reconstitution of Morphogen Signaling Gradients in Cultured Cells.

  2020cites this paper
• Ligand-receptor promiscuity enables cellular addressing

  2020cites this paper
• Synthetic tissue engineering: Programming multicellular self-organization by designing customized cell-cell communication

  2020cites this paper
• Synthetic regulation of multicellular systems for regenerative engineering

  2020cites this paper
• Patterning and growth control in vivo by an engineered GFP gradient

  2020cites this paper
• Engineering synthetic morphogen systems that can program multicellular patterning

  2020cites this paper
• Reconstituting tissue patterning

  2020cites this paper
• Synthetic Developmental Biology: Understanding Through Reconstitution.

  2020cites this paper