Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Current Protocols in Molecular Biology

• Publication date

  2007-04-01

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1002/0471142727.mb0116s78PMID 18265390
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Multicopy plasmid modification with phage lambda Red recombineering.

  2007cited by this paper
• Multicopy Plasmid Modification with Phage λ Red Recombineering

  2007influential reference
• A set of recombineering plasmids for gram-negative bacteria.

  2006influential reference
• Recombineering in Prokaryotes

  2005cited by this paper
• Simple and highly efficient BAC recombineering using galK selection

  2005cited by this paper
• In vivo recombineering of bacteriophage λ by PCR fragments and single-strand oligonucleotides

  2004influential reference
• In vivo recombineering of bacteriophage lambda by PCR fragments and single-strand oligonucleotides.

  2004cited by this paper
• Bacterial artificial chromosome engineering.

  2004cited by this paper
• Recombineering with overlapping single-stranded DNA oligonucleotides: Testing a recombination intermediate

  2003influential reference
• Mini-lambda: a tractable system for chromosome and BAC engineering.

  2003cited by this paper
• Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants.

  2003influential reference
• Genetic engineering using homologous recombination.

  2002cited by this paper
• Cell toxicity caused by products of the p(L) operon of bacteriophage lambda.

  2001cited by this paper
• High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides

  2001cited by this paper
• A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

  2001cited by this paper
• Recombineering: a powerful new tool for mouse functional genomics

  2001cited by this paper
• One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

  2000cited by this paper
• An efficient recombination system for chromosome engineering in Escherichia coli.

  2000influential reference
• PCR-mediated gene replacement in Escherichia coli.

  2000cited by this paper
• DNA cloning by homologous recombination in Escherichia coli

  2000cited by this paper
• Rapid engineering of bacterial artificial chromosomes using oligonucleotides

  2000cited by this paper
• Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.

  1995cited by this paper
• Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.

  1992cited by this paper
• Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature‐sensitive pSC101 replicon

  1991cited by this paper
• Basic local alignment search tool.

  1990cited by this paper
• Chromosomal transformation of Escherichia coli recD strains with linearized plasmids

  1989cited by this paper
• Synthesis of linear plasmid multimers in Escherichia coli K-12

  1986cited by this paper
• Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria

  1985cited by this paper
• Experimental Methods for Use with Lambda

  1983cited by this paper
• Selection for loss of tetracycline resistance by Escherichia coli

  1981cited by this paper
• Positive selection for loss of tetracycline resistance

  1980cited by this paper
• Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions

  1975cited by this paper

• Engineering the Self-assembly of Bacterial Microcompartment Shell Proteins via Charged Mutations

  2026cites this paper
• Genetically programmable wearable devices for precision physiological and molecular monitoring

  2025cites this paper
• Gene regulation, phenotypic memory, and selection in fluctuating environments

  2025cites this paper
• A High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System

  2025cites this paper
• Importance of Target Gene Locus on the Stability of Recombinant Viruses in the Baculovirus Expression System

  2025cites this paper
• A high throughput assay for measuring secreted protein based on a de novo fluorescent reporter reveals regulatory and structural insights in Salmonella type three secretion system

  2025cites this paper
• “A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in Salmonella”

  2025cites this paper
• Infection characteristics among Serratia marcescens capsule lineages

  2024cites this paper
• The inactivation of tolC sensitizes Escherichia coli to perturbations in lipopolysaccharide transport

  2024cites this paper
• Characterization and engineering of the type 3 secretion system needle monomer from Salmonella through the construction and screening of a comprehensive mutagenesis library

  2024cites this paper
• Robust control of replication initiation in the absence of DnaA-ATP ↔ DnaA-ADP regulatory elements in Escherichia coli

  2024cites this paper
• Spatial propagation of temperate phages within and among biofilms

  2024cites this paper
• Modular Assembly of Synthetic Secondary Chromosomes.

  2024cites this paper
• High-throughput DNA engineering by mating bacteria

  2024cites this paper
• Conjugative type IV secretion systems enable bacterial antagonism that operates independently of plasmid transfer

  2024cites this paper
• DNA Packaging Specificity in the λ‐Like Phages: Gifsy‐1

  2024cites this paper
• The crystal structure of bacteriophage λ RexA provides novel insights into the DNA binding properties of Rex-like phage exclusion proteins

  2023cites this paper
• The sensor of the bacterial histidine kinase CpxA is a novel dimer of extracytoplasmic Per-ARNT-Sim domains

  2023cites this paper
• Oxidative stress drives mutagenesis through transcription-coupled repair in bacteria

  2023cites this paper
• Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster

  2023cites this paper
• D-Serine reduces the expression of the cytopathic genotoxin colibactin

  2023cites this paper
• Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

  2023cites this paper
• Recent Advances in Genetically Engineered Microorganisms and their Risks: A Review

  2023cites this paper
• Construction of a constitutively active type III secretion system for heterologous protein secretion

  2023cites this paper
• A strain of Vibrio alginolyticus isolated from Azumapecten farreri and its pathogenic mechanism using CRISPR-Cas9 technology

  2023cites this paper
• Conserved metabolic regulator ArcA responds to oxygen availability, iron limitation, and cell envelope perturbations during bacteremia

  2023cites this paper
• Modern Approaches to the Genome Editing of Antibiotic Biosynthetic Clusters in Actinomycetes

  2023cites this paper
• Trade-offs constrain adaptive pathways to the type VI secretion system survival

  2023cites this paper
• The arm of the starfish: The far-reaching applications of Patiria miniata as a model system in evolutionary, developmental, and regenerative biology.

  2022cites this paper
• Deletion of prophages from the Clostridioides difficile 630 genome

  2022cites this paper
• Contributions of F‐specific subunits to the F plasmid‐encoded type IV secretion system and F pilus

  2022cites this paper
• Trade-offs constrain adaptive pathways to T6 survival

  2022cites this paper
• Whole-Genome Sequencing of a Chlorimuron-Ethyl-Degrading Strain: Chenggangzhangella methanolivorans CHL1 and Its Degrading Enzymes

  2022cites this paper
• Impaired KDM2B-mediated PRC1 recruitment to chromatin causes defective neural stem cell self-renewal and ASD/ID-like behaviors

  2022cites this paper
• Development of a New Recombineering System for Agrobacterium Species

  2022cites this paper
• Author Correction: Structure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids

  2022cites this paper
• Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation

  2022cites this paper
• Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase

  2022cites this paper
• Profiling the intragenic toxicity determinants of toxin–antitoxin systems: revisiting hok/Sok regulation

  2022influential citation
• Nucleotide excision repair is universally mutagenic and 5 transcription-associated 6

  2022cites this paper
• Expression of the DeaD RNA Helicase Is Regulated at Multiple Levels through Its Long mRNA 5′ Untranslated Region

  2022cites this paper
• Homologs of the Escherichia coli F Element Protein TraR, Including Phage Lambda Orf73, Directly Reprogram Host Transcription

  2022cites this paper
• Bacteriophage self-counting in the presence of viral replication

  2021cites this paper
• Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants

  2021cites this paper
• Loss of histone methyltransferase ASH1L in the developing mouse brain causes autistic-like behaviors

  2021cites this paper
• Genetic Modification of Sodalis Species by DNA Transduction

  2021cites this paper
• A plasmid toolbox for controlled gene expression across the Proteobacteria

  2021cites this paper
• Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae

  2021cites this paper
• The Atypical Antipsychotic Quetiapine Induces Multiple Antibiotic Resistance in Escherichia coli

  2021cites this paper
• Vertex protein PduN tunes encapsulated pathway performance by dictating bacterial metabolosome morphology

  2021cites this paper
• Development of a BoHV-4 viral vector expressing tgD of BoHV-1 and evaluation of its immunogenicity in mouse model

  2021cites this paper
• A standardized genome architecture for bacterial synthetic biology (SEGA)

  2021cites this paper
• Distinct patterns of mutational sensitivity for λ resistance and maltodextrin transport in Escherichia coli LamB

  2020cites this paper
• DNA Cloning and Assembly: Methods and Protocols

  2020cites this paper
• Chemical-genetic interrogation of RNA polymerase mutants reveals structure-function relationships and physiological tradeoffs

  2020cites this paper
• Loss of ASH1L in developing brains causes autistic-like behaviors in a mouse model

  2020cites this paper
• DNA transduction in Sodalis species: implications for the genetic modification of uncultured endosymbionts of insects

  2020cites this paper
• Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics

  2020cites this paper
• An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system

  2020cites this paper
• Reporter Phage-Based Detection of Bacterial Pathogens: Design Guidelines and Recent Developments

  2020cites this paper
• Predicting Drug Resistance Using Deep Mutational Scanning

  2020cites this paper
• Rapid diffusion of large TatA complexes detected using single particle tracking microscopy

  2020cites this paper
• Bone formation potential of collagen type I-based recombinant peptide particles in rat calvaria defects☆

  2020cites this paper
• Genetic manipulation of phages for therapy using BRED.

  2020cites this paper
• Uncoupling of invasive bacterial mucosal immunogenicity from pathogenicity

  2020cites this paper
• Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida

  2020cites this paper
• YejM Modulates Activity of the YciM/FtsH Protease Complex To Prevent Lethal Accumulation of Lipopolysaccharide

  2020cites this paper
• Identification of Residues Essential for HSV ICP8 Mediated Annealing of ssDNA

  2020cites this paper
• Mutations induced by Bleomycin, 4-nitroquinoline-1-oxide, and hydrogen peroxide in the rpoB gene of Escherichia coli: Perspective on Mutational Hotspots.

  2020cites this paper
• A genomic integration platform for heterologous cargo encapsulation in 1,2-propanediol utilization bacterial microcompartments

  2020cites this paper
• Author Correction: Uncoupling of invasive bacterial mucosal immunogenicity from pathogenicity

  2020cites this paper
• Chromosomal barcoding of E. coli populations reveals lineage diversity dynamics at high resolution

  2019cites this paper
• Techniques for analyzing gene expression using BAC-based reporter constructs.

  2019cites this paper
• EXPANDING MOLECULAR TOOLS FOR THE METABOLIC ENGINEERING OF RALSTONIA EUTROPHA H16

  2019cites this paper
• Measuring Transcription at a Single Gene Copy Reveals Hidden Drivers of Bacterial Individuality

  2019cites this paper
• Transcriptomic analysis of Chlorimuron-ethyl degrading bacterial strain Klebsiella jilinsis 2N3.

  2019cites this paper
• Bovine Herpesvirus type 4 ( BoHV-4 ) Vector Delivering Nucleocapsid 1 Protein of Crimean Congo Hemorrhagic Fever Virus Induces Comparable 2 Protective Immunity against Lethal Challenge in IFNAR-/-Mice Model 3 4

  2019cites this paper
• Modification of T2 phage infectivity toward Escherichia coli O157:H7 via using CRISPR/Cas9

  2019cites this paper
• The Cytoplasm-Entry Domain of Antibacterial CdiA Is a Dynamic α-Helical Bundle with Disulfide-Dependent Structural Features.

  2019cites this paper
• Convergent Evolution of the Barnase/EndoU/Colicin/RelE (BECR) Fold in Antibacterial tRNase Toxins.

  2019cites this paper
• Modulated efficacy CRISPRi reveals evolutionary conservation of essential gene expression-fitness relationships in bacteria

  2019cites this paper
• Impaired Efflux of the Siderophore Enterobactin Induces Envelope Stress in Escherichia coli

  2019cites this paper
• A New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes

  2019cites this paper
• Techniques for chromosomal integration and expression optimization in Escherichia coli.

  2018cites this paper
• An economically and environmentally acceptable synthesis of chiral drug intermediate l-pipecolic acid from biomass-derived lysine via artificially engineered microbes

  2018cites this paper
• Phage-Based Applications in Synthetic Biology.

  2018cites this paper
• Selection-Enhanced Mutagenesis of lac Genes Is Due to Their Coamplification with dinB Encoding an Error-Prone DNA Polymerase

  2018cites this paper
• Simultaneous Binding of Multiple EF-Tu Copies to Translating Ribosomes in Live Escherichia coli

  2018cites this paper
• SpoT and GppA hydrolases prevent the gratuitous activation of RelA by pppGpp in Escherichia coli

  2018cites this paper
• Functional plasticity of antibacterial EndoU toxins

  2018cites this paper
• ANALYSIS OF A FUNCTIONAL HETEROLOGOUS CHROMOSOMAL REPLICATION ORIGIN TRANSPLANT IN E. coli

  2018cites this paper
• The influence of sequence context on the evolution of bacterial gene expression

  2018cites this paper
• sRNA Target Prediction Organizing Tool (SPOT) Integrates Computational and Experimental Data To Facilitate Functional Characterization of Bacterial Small RNAs

  2018cites this paper
• Chromosomal barcoding as a tool for multiplexed phenotypic characterization of laboratory evolved lineages

  2018cites this paper
• Transcriptional profiling of isogenic Friedreich ataxia neurons and effect of an HDAC inhibitor on disease signatures

  2018cites this paper
• A synthetic system that senses Candida albicans and inhibits virulence factors

  2018cites this paper
• Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis.

  2018cites this paper
• Transcriptional profiling of isogenic Friedreich ataxia induced pluripotent stem cell-derived neurons

  2018cites this paper
• The Role of Phage tRNAs in the Evolution of Codon Usage Biases in Giant Pseudomonas Phage phiKZ and EL

  2017cites this paper
• Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact‐dependent translocation

  2017influential citation