Inhibition of interleukin 8 attenuates angiogenesis in bronchogenic carcinoma

We investigated the role of interleukin 8 (IL-8) in mediating angiogenesis in human bronchogenic carcinoma. Increased quantities of IL-8 were detected in tumor tissue as compared with normal lung tissue. Immunohistochemical staining of tumors revealed primary localization of IL-8 to individual tumor cells and demonstrated the capacity of tumor to elaborate IL-8. Functional studies that used tissue homogenates of tumors demonstrated the induction of both in vitro endothelial cell chemotaxis and in vivo corneal neovascularization. It is important to note that the addition of neutralizing antisera to IL-8 to these assays resulted in the marked and specific attenuation of these responses. Our observations definitively establish IL-8 as a primary mediator of angiogenesis in bronchogenic carcinoma and offer a potential target for immunotherapies against solid malignancies.

• Publication year

  1994

• Venue

  Journal of Experimental Medicine

• Publication date

  1994-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.179.5.1409PMID 7513008PMCID 2191482
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Tumor angiogenesis

  2020cited by this paper
• Interleukln-8 stimulates angiogenesis in rats

  1993cited by this paper
• The production of interleukin-1 receptor antagonist by human bronchogenic carcinoma.

  1993cited by this paper
• Interleukin-8 is produced in neoplastic and infectious diseases of the human central nervous system.

  1992cited by this paper
• Interleukin-8 as a macrophage-derived mediator of angiogenesis.

  1992cited by this paper
• American Cancer Society textbook of clinical oncology

  1991cited by this paper
• Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8

  1991cited by this paper
• Thiol-containing compounds inhibit the production of monocyte/macrophage-derived angiogenic activity

  1991cited by this paper
• IL-1 and TNF-alpha induction of IL-8 and monocyte chemotactic and activating factor (MCAF) mRNA expression in a human astrocytoma cell line.

  1991cited by this paper
• Cytokine production by the bladder carcinoma cell line 5637: rapid analysis of mRNA expression levels using a cDNA-PCR procedure.

  1991cited by this paper
• Interleukin-8 gene expression by a pulmonary epithelial cell line. A model for cytokine networks in the lung.

  1990influential reference
• Coding region structure of interleukin-8 gene of human lung giant cell carcinoma LU65C cells that produce LUCT/interleukin-8: homogeneity in interleukin-8 genes.

  1990cited by this paper
• Neutrophil chemotactic factor (IL-8) gene expression by cytokine-treated retinal pigment epithelial cells.

  1990cited by this paper
• Cytokine-induced gene expression of a neutrophil chemotactic factor/IL-8 in human hepatocytes.

  1990cited by this paper
• Human alveolar macrophage gene expression of interleukin-8 by tumor necrosis factor-alpha, lipopolysaccharide, and interleukin-1 beta.

  1990cited by this paper
• Potent and specific inhibition of IL-8-, IL-1 alpha- and IL-1 beta-induced in vitro human lymphocyte migration by calcium channel antagonists.

  1989cited by this paper
• Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-alpha, LPS, and IL-1 beta.

  1989cited by this paper
• Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils.

  1989cited by this paper
• Monokine-induced neutrophil chemotactic factor gene expression in human fibroblasts.

  1989cited by this paper
• Induction of angiogenesis during the transition from hyperplasia to neoplasia

  1989cited by this paper
• The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes.

  1989cited by this paper
• Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP).

  1988cited by this paper
• Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor

  1988cited by this paper
• Stimulation of neovascularization by human rheumatoid synovial tissue macrophages.

  1986cited by this paper
• Migration of capillary endothelial cells is stimulated by tumour-derived factors

  1980cited by this paper
• Acquisition of angiogenic capacity and neoplastic transformation in the rat mammary gland.

  1978cited by this paper
• Activated macrophages induce vascular proliferation

  1977cited by this paper
• Growth stimulation of endothelial cells by simultaneous culture with Sarcoma 180 cells in diffusion chambers.

  1977cited by this paper
• Growth stimulation of cultured endothelial cells by tumor cell homogenates.

  1976cited by this paper
• Relation of vascular proliferation to tumor growth.

  1976cited by this paper

• The role of IL-8 in cancer development and its impact on immunotherapy resistance.

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• Inflammatory cytokines and specific factors influencing lung cancer progression

  2025cites this paper
• Immune Cell Migration to Cancer

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  2018cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2014cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012influential citation
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  2012cites this paper
• Tissue Biomarkers in Renal Cell Carcinoma: Intermediate Endpoints in the Selection of Targeted Agents for RCC

  2012cites this paper
• Angiogenic activity of classical hematopoietic cytokines.

  2012cites this paper
• Chemokines as mediators of tumor angiogenesis and neovascularization.

  2011cites this paper
• Rôle des progéniteurs dans l'hypertension artérielle pulmonaire humaine et expérimentale

  2011cites this paper
• Epidermal growth factor receptor reactivation induced by E-prostanoid-3 receptor- and tumor necrosis factor-alpha-converting enzyme-dependent feedback exaggerates interleukin-8 production in airway cancer (NCI-H292) cells.

  2011cites this paper
• Targeting of the innate immunity/inflammation as complementary anti-tumor therapies

  2011cites this paper
• The role of chemokines and their receptors in angiogenesis

  2011cites this paper
• Procathepsin D and cytokines influence the proliferation of lung cancer cells.

  2011cites this paper
• High tumor levels of IL6 and IL8 abrogate preclinical efficacy of the γ‐secretase inhibitor, RO4929097

  2011cites this paper
• Physiological and pathological angiogenesis in the adult pulmonary circulation.

  2011cites this paper
• ERβ and PEA3 co-activate IL-8 expression and promote the invasion of breast cancer cells

  2011cites this paper
• Importance of Serum Il-8 and Rantes as Markers for Breast Cancer Progression

  2011cites this paper
• Tumor cell-specific bioluminescence platform to identify stroma-induced changes to anti-cancer drug activity

  2010cites this paper
• IL-1ß (+3953 C/T) and IL-8 (-251 A/T) gene polymorphisms in H. pylori mediated gastric disorders.

  2010cites this paper
• Inflammatory Cytokines and Their Role in Bone Metastasis and Osteolysis

  2010cites this paper
• CXC chemokines in cancer angiogenesis and metastases.

  2010cites this paper
• Chemokines.

  2010cites this paper
• Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment

  2010cites this paper
• Interleukin-8 mediates resistance to antiangiogenic agent sunitinib in renal cell carcinoma.

  2010cites this paper
• Angiogenesis and Lymphangiogenesis of Gastric Cancer

  2010cites this paper
• Chemokines in angiogenesis.

  2010cites this paper
• The Immune Response to Non-Small Cell Lung Cancer

  2010cites this paper
• IL-8和MMP-9在非小细胞肺癌患者组织及血清中表达水平的研究

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  2010cites this paper
• CHFR, a potential tumor suppressor, downregulates interleukin-8 through the inhibition of NF-κB

  2009cites this paper
• The role of chemokines in cancer immune surveillance by the adaptive immune system.

  2009cites this paper
• Chemokines in Lung Cancer Metastasis

  2009cites this paper
• Preclinical and clinical studies on the co-regulation of tumor-induced angiogenesis and dendritic cell suppression

  2009cites this paper
• Dendritic cell-activated cytokine-induced killer cells enhance the anti-tumor effect of chemotherapy on non-small cell lung cancer in patients after surgery.

  2009cites this paper
• Angiogenesis and Angiogenic Diversity in Lung Cancer Metastasis

  2009cites this paper
• Anti-angiogenic actions of pyrrolidine dithiocarbamate, a nuclear factor kappa B inhibitor

  2009cites this paper
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• Monocyte chemotactic protein 1 promotes lung cancer-induced bone resorptive lesions in vivo.

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• Chemokines in tumor-associated angiogenesis

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• CXC Chemokines in Cancer Angiogenesis

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