RNA silencing: Methods and protocols

No abstract is available for this paper.

• Publication year

  2006

• Venue

  Methods in Molecular Biology™

• Publication date

  2006-05-01

• Fields of study

  Biology, Psychology

• Identifiers
  DOI 10.1385/1592599354
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• MicroRNAs Play an Essential Role in the Development of Cardiac Hypertrophy

  2007cited by this paper
• Measurement of the interleukin family member ST2 in patients with acute dyspnea: results from the PRIDE (Pro-Brain Natriuretic Peptide Investigation of Dyspnea in the Emergency Department) study.

  2007cited by this paper
• Comparison of midregional pro-atrial natriuretic peptide with N-terminal pro-B-type natriuretic peptide in predicting survival in patients with chronic heart failure.

  2007cited by this paper
• Expression of microRNAs is dynamically regulated during cardiomyocyte hypertrophy.

  2007influential reference
• Prognostic utility of growth differentiation factor-15 in patients with chronic heart failure.

  2007cited by this paper
• The relationship between non-protein-coding DNA and eukaryotic complexity.

  2007cited by this paper
• Altered microRNA expression in human heart disease.

  2007cited by this paper
• Comparative evaluation of B-type natriuretic peptide, mid-regional pro-A-type natriuretic peptide, mid-regional pro-adrenomedullin, and Copeptin to predict 1-year mortality in patients with acute destabilized heart failure.

  2007cited by this paper
• Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2.

  2007cited by this paper
• Utility of amino-terminal pro-brain natriuretic peptide, galectin-3, and apelin for the evaluation of patients with acute heart failure.

  2006cited by this paper
• Midregional pro-A-type natriuretic peptide measurements for diagnosis of acute destabilized heart failure in short-of-breath patients: comparison with B-type natriuretic peptide (BNP) and amino-terminal proBNP.

  2006cited by this paper
• Galectin-3 regulates myofibroblast activation and hepatic fibrosis.

  2006cited by this paper
• Acute Heart Failure Syndromes: Current State and Framework for Future Research

  2005cited by this paper
• Induction and suppression of RNA silencing: insights from viral infections

  2005influential reference
• Silencing of microRNAs in vivo with ‘antagomirs’

  2005cited by this paper
• MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies

  2005cited by this paper
• Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery

  2005cited by this paper
• A resource for large-scale RNA-interference-based screens in mammals

  2004influential reference
• Nuclear Export of MicroRNA Precursors

  2004cited by this paper
• Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component

  2004cited by this paper
• Probing the MicroRNA and Small Interfering RNA Pathways with Virus-Encoded Suppressors of RNA Silencing

  2004cited by this paper
• Molecular mechanism of RNA silencing suppression mediated by p19 protein of tombusviruses

  2004cited by this paper
• MicroRNA precursors in motion: exportin-5 mediates their nuclear export.

  2004cited by this paper
• Mechanisms of gene silencing by double-stranded RNA

  2004influential reference
• MicroRNAs Modulate Hematopoietic Lineage Differentiation

  2004cited by this paper
• Enzymatic production of RNAi libraries from cDNAs

  2004cited by this paper
• Restriction enzyme–generated siRNA (REGS) vectors and libraries

  2004cited by this paper
• The functions of animal microRNAs

  2004cited by this paper
• Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step.

  2004cited by this paper
• RNase III enzymes and the initiation of gene silencing

  2004cited by this paper
• Small interfering RNA production by enzymatic engineering of DNA (SPEED).

  2004cited by this paper
• Galectin-3 Marks Activated Macrophages in Failure-Prone Hypertrophied Hearts and Contributes to Cardiac Dysfunction

  2004cited by this paper
• Genetic analysis of RNA-mediated transcriptional gene silencing.

  2004influential reference
• Rational siRNA design for RNA interference

  2004cited by this paper
• Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

  2004cited by this paper
• Probing the precision of the mitotic clock with a live-cell fluorescent biosensor

  2004cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• Argonaute2 Is the Catalytic Engine of Mammalian RNAi

  2004cited by this paper
• Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells

  2004cited by this paper
• Use of B-type natriuretic peptide in the evaluation and management of acute dyspnea.

  2004cited by this paper
• RNA silencing: no mercy for viruses?

  2004influential reference
• A large-scale RNAi screen in human cells identifies new components of the p53 pathway

  2004influential reference
• Effects and side-effects of viral RNA silencing suppressors on short RNAs.

  2004cited by this paper
• Regulation of Flowering Time and Floral Organ Identity by a MicroRNA and Its APETALA2-Like Target Genes Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.016238.

  2003cited by this paper
• Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing

  2003influential reference
• Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.

  2003cited by this paper
• Functional siRNAs and miRNAs exhibit strand bias.

  2003cited by this paper
• Role of MicroRNAs in Plant and Animal Development

  2003cited by this paper
• Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-κB

  2003influential reference
• The nuclear RNase III Drosha initiates microRNA processing

  2003cited by this paper
• Identification of Serum Soluble ST2 Receptor as a Novel Heart Failure Biomarker

  2003cited by this paper
• Adenosine Kinase Is Inactivated by Geminivirus AL2 and L2 Proteins Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.015180.

  2003influential reference
• P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction.

  2003cited by this paper
• Asymmetry in the Assembly of the RNAi Enzyme Complex

  2003influential reference
• An approach to genomewide screens of expressed small interfering RNAs in mammalian cells

  2003cited by this paper
• Evidence for Nuclear Processing of Plant Micro RNA and Short Interfering RNA Precursors1[w]

  2003cited by this paper
• siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells.

  2003influential reference
• Size selective recognition of siRNA by an RNA silencing suppressor.

  2003cited by this paper
• Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors.

  2003cited by this paper
• RNAi targeting of DNA virus in plants

  2003cited by this paper
• The fragile X syndrome repeats form RNA hairpins that do not activate the interferon-inducible protein kinase, PKR, but are cut by Dicer.

  2003cited by this paper
• Specificity of short interfering RNA determined through gene expression signatures

  2003cited by this paper
• The capacity of transgenic tobacco to send a systemic RNA silencing signal depends on the nature of the inducing transgene locus.

  2003cited by this paper
• The Coat Protein of Turnip Crinkle Virus Suppresses Posttranscriptional Gene Silencing at an Early Initiation Step

  2003cited by this paper
• Geminivirus AL2 and L2 Proteins Interact with and Inactivate SNF1 Kinase Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009530.

  2003influential reference
• A gene encoding an RNase D exonuclease-like protein is required for post-transcriptional silencing in Arabidopsis.

  2003cited by this paper
• Identification of a minimal sequence required for activation of the tomato golden mosaic virus coat protein promoter in protoplasts.

  2003cited by this paper
• Expression profiling reveals off-target gene regulation by RNAi

  2003cited by this paper
• Functional Characterization of the Nuclear Localization Signal for a Suppressor of Posttranscriptional Gene Silencing

  2003influential reference
• Low temperature inhibits RNA silencing‐mediated defence by the control of siRNA generation

  2003cited by this paper
• Selective regulation of neurite extension and synapse formation by the beta but not the alpha isoform of CaMKII.

  2003cited by this paper
• Insight into the mechanism of the peptide-based gene delivery system MPG: implications for delivery of siRNA into mammalian cells.

  2003cited by this paper
• Genomewide view of gene silencing by small interfering RNAs

  2003cited by this paper
• High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants

  2002cited by this paper
• Contribution of downstream promoter elements to transcriptional regulation of the rice tungro bacilliform virus promoter.

  2002cited by this paper
• A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

  2002cited by this paper
• Functional siRNA expression from transfected PCR products.

  2002cited by this paper
• Ribonuclease activity and RNA binding of recombinant human Dicer

  2002cited by this paper
• U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells

  2002influential reference
• Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells

  2002cited by this paper
• Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.

  2002cited by this paper
• Mutation of three cysteine residues in Tomato yellow leaf curl virus-China C2 protein causes dysfunction in pathogenesis and posttranscriptional gene-silencing suppression.

  2002cited by this paper
• Effective expression of small interfering RNA in human cells

  2002cited by this paper
• Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP

  2002influential reference
• MicroRNA maturation: stepwise processing and subcellular localization

  2002cited by this paper
• Mouse ribonuclease III. cDNA structure, expression analysis, and chromosomal location

  2002cited by this paper
• Cleavage of Scarecrow-like mRNA Targets Directed by a Class of Arabidopsis miRNA

  2002cited by this paper
• RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells

  2002cited by this paper
• Viral suppression of systemic silencing.

  2002cited by this paper
• A viral suppressor of RNA silencing differentially regulates the accumulation of short interfering RNAs and micro-RNAs in tobacco

  2002cited by this paper
• A viral protein inhibits the long range signaling activity of the gene silencing signal

  2002cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Minireview Micrornas: Tiny Regulators with Great Potential

  2001cited by this paper
• RNA silencing as a plant immune system against viruses.

  2001influential reference
• Thirty-three years later, a glimpse at the ribonuclease III active site.

  2001cited by this paper
• A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA

  2001cited by this paper
• RNA interference is mediated by 21- and 22-nucleotide RNAs.

  2001influential reference
• Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

  2001influential reference
• Plants expressing tomato golden mosaic virus AL2 or beet curly top virus L2 transgenes show enhanced susceptibility to infection by DNA and RNA viruses.

  2001cited by this paper
• Antiviral Actions of Interferons

  2001cited by this paper

• Plant viral disease management-from cross protection to CRISPR

  2025cites this paper
• The Emerging Role of Omics-Based Approaches in Plant Virology

  2025cites this paper
• Strategies for Engineering Virus Resistance in Potato

  2023cites this paper
• Nitrate reductase is required for sclerotial development and virulence of Sclerotinia sclerotiorum

  2023cites this paper
• Phenotypic and genomic changes during Turnip mosaic virus adaptation to Arabidopsis thaliana mutants lacking epigenetic regulatory factors

  2023cites this paper
• Interference of Small RNAs in Fusarium graminearum through FgGMTV1 Infection

  2022cites this paper
• Biochemical Profile of Transgenic Banana cv. Rasthali (Musa spp.) Engineered for Resistance Against Fusarium Wilt

  2022cites this paper
• RNA Interference: Promising Approach to Combat Plant Viruses

  2022cites this paper
• Similar Characteristics of siRNAs of Plant Viruses Which Replicate in Plant and Fungal Hosts

  2022influential citation
• Genome Engineering Technology for Durable Disease Resistance: Recent Progress and Future Outlooks for Sustainable Agriculture

  2022cites this paper
• Tools and targets: The dual role of plant viruses in CRISPR–Cas genome editing

  2022cites this paper
• Identification of Silencing Suppressor Protein Encoded by Strawberry Mottle Virus

  2022cites this paper
• Beet curly top Iran virus Rep and V2 gene work as silencing suppressors through separate mechanisms

  2022cites this paper
• Targeting of genomic and negative‐sense strands of viral RNA contributes to antiviral resistance mediated by artificial miRNAs and promotes the emergence of complex viral populations

  2022cites this paper
• Uncovering Novel Viral Innate Immune Evasion Strategies: What Has SARS-CoV-2 Taught Us?

  2022cites this paper
• Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1

  2021cites this paper
• Screening of Key Proteins Affecting Floral Initiation of Saffron Under Cold Stress Using iTRAQ-Based Proteomics

  2021cites this paper
• GhPLP2 Positively Regulates Cotton Resistance to Verticillium Wilt by Modulating Fatty Acid Accumulation and Jasmonic Acid Signaling Pathway

  2021cites this paper
• Composted Municipal Green Waste Infused with Biocontrol Agents to Control Plant Parasitic Nematodes—A Review

  2021cites this paper
• Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology

  2021influential citation
• The Potyviral Protein 6K1 Reduces Plant Proteases Activity during Turnip mosaic virus Infection

  2021cites this paper
• Dynamics of DNA Methylation and Its Functions in Plant Growth and Development

  2021cites this paper
• Genetically Modified Crops: Current Status, Prospects and Challenges Volume 2

  2021cites this paper
• RNA-Based Technologies for Engineering Plant Virus Resistance

  2021cites this paper
• The role of Dicer-dependent RNA interference in regulating cross-species communication during fungus-fungus interactions

  2021cites this paper
• Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens

  2021cites this paper
• GhWRKY70D13 Regulates Resistance to Verticillium dahliae in Cotton Through the Ethylene and Jasmonic Acid Signaling Pathways

  2020cites this paper
• The Induction of an Effective dsRNA-Mediated Resistance Against Tomato Spotted Wilt Virus by Exogenous Application of Double-Stranded RNA Largely Depends on the Selection of the Viral RNA Target Region

  2020cites this paper
• Opportunities and Challenges in Studies of Host-Pathogen Interactions and Management of Verticillium dahliae in Tomatoes

  2020cites this paper
• Role of the methionine cycle in the temperature‐sensitive responses of potato plants to potato virus Y

  2020cites this paper
• Bymovirus-induced yellow mosaic diseases in barley and wheat: viruses, genetic resistances and functional aspects

  2020cites this paper
• Application of Exogenous dsRNAs-induced RNAi in Agriculture: Challenges and Triumphs

  2020influential citation
• Potato Virus Y Emergence and Evolution from the Andes of South America to Become a Major Destructive Pathogen of Potato and Other Solanaceous Crops Worldwide

  2020cites this paper
• Small RNAs: Big Roles in White Mold Infecting Crop Plants

  2020cites this paper
• Opportunities and Challenges to Understand Host-Pathogen Interactions and Management of Verticillium dahliae on Tomato

  2020cites this paper
• Structure and Double-Stranded RNA-Binding Activity of the Birnavirus Drosophila X Virus VP3 Protein

  2020cites this paper
• The Arabidopsis active demethylase ROS1 cis-regulates immune-responsive genes by pruning DNA methylation at promoter-regulatory regions

  2020cites this paper
• Differential expression of genes contributing to PCD triggered by exogenous oxalic acid in tomato (Solanum lycopersicum)

  2020cites this paper
• Different Genetic Sources Contribute to the Small RNA Population in the Arbuscular Mycorrhizal Fungus Gigaspora margarita

  2020cites this paper
• Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity

  2020cites this paper
• Interaction of a plant virus protein with the signature Cajal body protein coilin facilitates salicylic acid-mediated plant defence responses.

  2019cites this paper
• The Role of the Second Subunit of the Origin Recognition Complex (ORC2) in the Development of Arabidopsis thaliana

  2019cites this paper
• Volatile Composition and Biological Activities of the Leaf Essential Oil from Zanthoxylum limoncello Grown in Oaxaca, México

  2019cites this paper
• Development of an RNA interference ( RNAi ) gene knockdown protocol 13 in the anaerobic gut fungus Pecoramyces ruminantium strain C 1 A 14 15

  2019cites this paper
• Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives1[OPEN]

  2019cites this paper
• Insight into TaWRKYs transcription factors role during wheat growth and Septoria defense.

  2019influential citation
• Emerging Viruses in Bees: From Molecules to Ecology.

  2018cites this paper
• MIGS as a Simple and Efficient Method for Gene Silencing in Rice

  2018influential citation
• Mycoviruses as Triggers and Targets of RNA Silencing in White Mold Fungus Sclerotinia sclerotiorum

  2018cites this paper
• Characterization of circular DNA molecules from cotton plants with leaf curl disease

  2018cites this paper
• Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption

  2018cites this paper
• The function of Arabidopsis microRNAs in defense against the necrotrophic fungal pathogen Alternaria brassicicola

  2018influential citation
• Synergistic Binding of bHLH Transcription Factors to the Promoter of the Maize NADP-ME Gene Used in C4 Photosynthesis Is Based on an Ancient Code Found in the Ancestral C3 State

  2018influential citation
• Validation of monoclonal antibodies in detection of cassava brown streak viruses using triple antibody sandwich enzyme-linked immunoassay technique

  2018cites this paper
• Characterization of P1 leader proteases of the Potyviridae family and identification of the host factors involved in their proteolytic activity during viral infection

  2018influential citation
• Chinese Wheat Mosaic Virus-Induced Gene Silencing in Monocots and Dicots at Low Temperature

  2018cites this paper
• Modulation of plant autophagy during pathogen attack.

  2018cites this paper
• Mitochondrial ferritin deficiency reduces male fertility in mice.

  2017cites this paper
• RNAi-mediated resistance to rice black-streaked dwarf virus in transgenic rice

  2017cites this paper
• Rhizomania - A Review

  2017cites this paper
• Cap analog and Potato virus A HC-Pro silencing suppressor improve GFP transient expression using an infectious virus vector in Nicotiana benthamiana

  2017cites this paper
• Transepithelial delivery of macromolecular biopesticides

  2017cites this paper
• MicroRNA-Mediated Gene Silencing in Plant Defense and Viral Counter-Defense

  2017influential citation
• Screening of cassava improved germplasm for potential resistance against cassava mosaic disease

  2017cites this paper
• Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression

  2017cites this paper
• Plant genetic engineering and genetically modified crop breeding: history and current status

  2017cites this paper
• Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection

  2017cites this paper
• RNAi induced silencing of pathogenicity genes of Fusarium spp. for vascular wilt management in tomato

  2017cites this paper
• Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded βC1.

  2016cites this paper
• Diverse Functions of Small RNAs in Different Plant–Pathogen Communications

  2016cites this paper
• Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants1[OPEN]

  2016influential citation
• Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants

  2016cites this paper
• CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis

  2016cites this paper
• TRANSFORMACIÓN DE TOMATE CON GENES INVOLUCRADOS EN INMUNIDAD VEGETAL PARA CONFERIR RESISTENCIA AMPLIA CONTRA BACTERIAS

  2016cites this paper
• Island Cotton Enhanced Disease Susceptibility 1 Gene Encoding a Lipase-Like Protein Plays a Crucial Role in Response to Verticillium dahliae by Regulating the SA Level and H2O2 Accumulation

  2016cites this paper
• Host-induced gene silencing compromises Verticillium wilt in tomato and Arabidopsis

  2016cites this paper
• Exogenous RNA interference exposes contrasting roles for sugar exudation in host-finding by plant pathogens.

  2016influential citation
• Next-Generation Sequencing and Genome Editing in Plant Virology

  2016cites this paper
• Integration of a hairpin RNA-encoding gene derived from Tobacco streak virus confers resistance in groundnut (Arachis hypogaea L.) against peanut stem necrosis disease

  2016cites this paper
• Novel functional microRNAs from virus-free and infected Vitis vinifera plants under water stress

  2016cites this paper
• Interplays between Soil-Borne Plant Viruses and RNA Silencing-Mediated Antiviral Defense in Roots

  2016cites this paper
• Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics

  2016cites this paper
• Numerical behavior of a fractional order dynamical model of RNA silencing

  2016cites this paper
• The role of DNA (de)methylation in immune responsiveness of Arabidopsis

  2016influential citation
• Artificial microRNA-derived resistance to Cassava brown streak disease

  2016cites this paper
• Graphical Abstract CG 17-6-MS

  2016cites this paper
• Dimeric artificial microRNAs mediate high resistance to RSV and RBSDV in transgenic rice plants

  2016cites this paper
• Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein

  2016cites this paper
• Improved Pathogenicity of a Beet Black Scorch Virus Variant by Low Temperature and Co-infection with Its Satellite RNA

  2016cites this paper
• Cooperative interaction of antimicrobial peptides with the interrelated immune pathways in plants

  2015cites this paper
• ECERIFERUM7 subunit of the exosome, SUPERKILLER complex and small RNA species regulate cuticular wax biosynthesis in Arabidopsis thaliana stems

  2015cites this paper
• Plant Gene Silencing

  2015cites this paper
• The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage

  2015cites this paper
• Proteins interacting with mitochondrial ATP-dependent Lon protease (MAP1) in Magnaporthe oryzae are involved in rice blast disease.

  2015cites this paper
• Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond

  2015cites this paper
• Different Dicer-like protein components required for intracellular and systemic antiviral silencing in Arabidopsis thaliana

  2015cites this paper
• Symptom recovery in virus-infected plants: Revisiting the role of RNA silencing mechanisms.

  2015cites this paper
• Plants as factories for the Production of Pharmaceutical recombinant proteins

  2015cites this paper
• RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite

  2015cites this paper
• MicroRNA-19a regulates lipopolysaccharide-induced endothelial cell apoptosis through modulation of apoptosis signal-regulating kinase 1 expression

  2015cites this paper