First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Insects’ exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial communities of four developmental stages of the fungivorous species Hoplothrips carpathicus (Thysanoptera: Phlaeothripidae), verification of the presence of Wolbachia, in silico prediction of metabolic potentials of the microorganisms, and sequencing its mitochondrial COI barcode. Taxonomy-based analysis indicated that the bacterial community of H. carpathicus contained 21 bacterial phyla. The most abundant phyla were Proteobacteria, Actinobacteria, Bacterioidetes and Firmicutes, and the most abundant classes were Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Betaproteobacteria, with different proportions in the total share. For pupa and imago (adult) the most abundant genus was Wolbachia, which comprised 69.95% and 56.11% of total bacterial population respectively. Moreover, similarity analysis of bacterial communities showed that changes in microbiome composition are congruent with the successive stages of H. carpathicus development. PICRUSt analysis predicted that each bacterial community should be rich in genes involved in membrane transport, amino acid metabolism, carbohydrate metabolism, replication and repair processes.

• Publication year

  2018

• Venue

  Scientific Reports

• Publication date

  2018-09-26

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/s41598-018-32747-xPMID 30258200PMCID 6158184
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Evidence for a conserved microbiota across the different developmental stages of Plodia interpunctella

  2019cited by this paper
• Near full-length 16S rRNA gene next-generation sequencing revealed Asaia as a common midgut bacterium of wild and domesticated Queensland fruit fly larvae

  2018cited by this paper
• Effects of diet type, developmental stage, and gut compartment in the gut bacterial communities of two Cerambycidae species (Coleoptera)

  2017cited by this paper
• Horizontal Transmission of Intracellular Insect Symbionts via Plants

  2017cited by this paper
• DNA Barcoding studies on Thrips in India: Cryptic species and Species complexes

  2017cited by this paper
• Microbial composition of spiny ants (Hymenoptera: Formicidae: Polyrhachis) across their geographic range

  2017cited by this paper
• New Insights into the Microbiota of Moth Pests

  2017cited by this paper
• Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality

  2017cited by this paper
• The functional microbiome of arthropods.

  2017cited by this paper
• The functional microbiome of arthropods

  2017cited by this paper
• Evidence of a bacterial core in the stored products pest Plodia interpunctella: the influence of different diets.

  2016cited by this paper
• Detection and Localization of Wolbachia in Thrips palmi Karny (Thysanoptera: Thripidae)

  2016cited by this paper
• Insecticidal Activity and Insect Repellency of Four Species of Sea Lily (Comatulida: Comatulidae) From Taiwan

  2016cited by this paper
• MetaCoMET: a web platform for discovery and visualization of the core microbiome

  2016cited by this paper
• DNA Barcode Analysis of Thrips (Thysanoptera) Diversity in Pakistan Reveals Cryptic Species Complexes

  2016cited by this paper
• Antibacterial and cytotoxic activities of wild mushroom Fomes fomentarius (L.) Fr., Polyporaceae

  2016cited by this paper
• Comparative Gut Microbiomes of Four Species Representing the Higher and the Lower Termites

  2016cited by this paper
• Variability of Bacterial Communities in the Moth Heliothis virescens Indicates Transient Association with the Host

  2016cited by this paper
• Effects of BmCPV Infection on Silkworm Bombyx mori Intestinal Bacteria

  2016cited by this paper
• Biodiversity and Activity of the Gut Microbiota across the Life History of the Insect Herbivore Spodoptera littoralis

  2016cited by this paper
• Wolbachia Associations with Insects: Winning or Losing Against a Master Manipulator

  2016cited by this paper
• Comparison of Bacterial Communities of Flower Thrips (Frankliniella tritici1) and Potato Psyllid (Bactericera cockerelli2 )

  2015cited by this paper
• Bacterial Communities and Midgut Microbiota Associated with Mosquito Populations from Waste Tires in East-Central Illinois

  2015cited by this paper
• Multiorganismal insects: diversity and function of resident microorganisms.

  2015cited by this paper
• Polyploidy versus endosymbionts in obligately thelytokous thrips

  2015cited by this paper
• Hoplothrips carpathicus P ELIKÁN , 1961 ( Thysanoptera : Phlaeothripidae ) – a new thrips species in the Polish fauna

  2015cited by this paper
• Hoplothrips carpathicus PELIKÁN, 1961 (Thysanoptera: Phlaeothripidae) – a new thrips species in the Polish fauna

  2015cited by this paper
• Beta-glucans from edible and medicinal mushrooms: Characteristics, physicochemical and biological activities

  2015cited by this paper
• Insect behaviour and the microbiome.

  2015cited by this paper
• Screen traps as an efficient method in faunal research on fungus-feeding thrips (Tubulifera: Phlaeothripidae)

  2015cited by this paper
• Loss of Wolbachia but not Cardinium in the invasive range of the Australian thrips species, Pezothrips kellyanus

  2015cited by this paper
• Isolation of Fungi and Bacteria Associated with the Guts of Tropical Wood-Feeding Coleoptera and Determination of Their Lignocellulolytic Activities

  2015cited by this paper
• Remarkable sexually dimorphic Aroidothrips longistylus newly recorded from China (Thysanoptera: Thripidae).

  2015cited by this paper
• Detection of Low-Level Cardinium and Wolbachia Infections in Culicoides

  2015cited by this paper
• Acquisition and Structuring of Midgut Bacterial Communities in Gypsy Moth (Lepidoptera: Erebidae) Larvae

  2014cited by this paper
• EVOLUTION OF ASEXUALITY VIA DIFFERENT MECHANISMS IN GRASS THRIPS (THYSANOPTERA: Aptinothrips)

  2014cited by this paper
• The mosquito microbiota influences vector competence for human pathogens.

  2014cited by this paper
• Vitamin B6 Generated by Obligate Symbionts Is Critical for Maintaining Proline Homeostasis and Fecundity in Tsetse Flies

  2014cited by this paper
• Insect Gut Bacterial Diversity Determined by Environmental Habitat, Diet, Developmental Stage, and Phylogeny of Host

  2014cited by this paper
• European medicinal polypores--a modern view on traditional uses.

  2014cited by this paper
• Metamorphosis of a Butterfly-Associated Bacterial Community

  2014cited by this paper
• Apoptotic properties of polysaccharide isolated from fruiting bodies of medicinal mushroom Fomes fomentarius in human lung carcinoma cell line

  2014cited by this paper
• Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera: Thripidae) via Next Generation Sequencing

  2014cited by this paper
• Arthropods and inherited bacteria: from counting the symbionts to understanding how symbionts count

  2013cited by this paper
• The gut microbiota of insects - diversity in structure and function.

  2013cited by this paper
• Bacteria Mediate Oviposition by the Black Soldier Fly, Hermetia illucens (L.), (Diptera: Stratiomyidae)

  2013cited by this paper
• Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences

  2013cited by this paper
• Data, information, knowledge and principle: back to metabolism in KEGG

  2013cited by this paper
• EMPeror: a tool for visualizing high-throughput microbial community data

  2013cited by this paper
• Distribution of Endosymbiotic Reproductive Manipulators Reflects Invasion Process and Not Reproductive System Polymorphism in the Little Fire Ant Wasmannia auropunctata

  2013cited by this paper
• Differences in the Structure of the Gut Bacteria Communities in Development Stages of the Chinese White Pine Beetle (Dendroctonus armandi)

  2013cited by this paper
• Three new phenyl-ethanediols from the fruiting bodies of the mushroom Fomes fomentarius

  2013cited by this paper
• Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies

  2013cited by this paper
• Actinobacteria as essential symbionts in firebugs and cotton stainers (Hemiptera, Pyrrhocoridae).

  2013cited by this paper
• Faculty Opinions recommendation of Drosophila microbiome modulates host developmental and metabolic homeostasis via insulin signaling.

  2012cited by this paper
• The Biological Observation Matrix (BIOM) format or: how I learned to stop worrying and love the ome-ome

  2012cited by this paper
• Arthropod Diversity in a Tropical Forest

  2012cited by this paper
• Mycangia of Ambrosia Beetles Host Communities of Bacteria

  2012cited by this paper
• High-Resolution Analysis of Gut Environment and Bacterial Microbiota Reveals Functional Compartmentation of the Gut in Wood-Feeding Higher Termites (Nasutitermes spp.)

  2012cited by this paper
• Endogenous Plant Cell Wall Digestion: A Key Mechanism in Insect Evolution

  2012cited by this paper
• PANDAseq: paired-end assembler for illumina sequences

  2012cited by this paper
• Functional diversity within the simple gut microbiota of the honey bee

  2012cited by this paper
• Large and linked in scientific publishing

  2012cited by this paper
• Symbiotic bacteria (Erwinia sp.) in the gut of Frankliniella occidentalis (Thysanoptera: Thripidae) do not affect its ability to transmit tospovirus

  2012cited by this paper
• Industrial dye decolorizing lignin peroxidase from Kocuria rosea MTCC 1532

  2012cited by this paper
• Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

  2012cited by this paper
• Behind every great ant, there is a great gut

  2012cited by this paper
• Do diet and taxonomy influence insect gut bacterial communities?

  2012cited by this paper
• Geographical and ecological stability of the symbiotic mid‐gut microbiota in European firebugs, Pyrrhocoris apterus (Hemiptera, Pyrrhocoridae)

  2012cited by this paper
• Comparative Evaluation of the Gut Microbiota Associated with the Below- and Above-Ground Life Stages (Larvae and Beetles) of the Forest Cockchafer, Melolontha hippocastani

  2012cited by this paper
• Low-diversity bacterial community in the gut of the fruitfly Drosophila melanogaster.

  2011cited by this paper
• Anti-infective properties of the melanin-glucan complex obtained from medicinal tinder bracket mushroom, Fomes fomentarius (L.: Fr.) Fr. (Aphyllophoromycetideae).

  2011cited by this paper
• Biodiversity of Active and Inactive Bacteria in the Gut Flora of Wood-Feeding Huhu Beetle Larvae (Prionoplus reticularis)

  2011cited by this paper
• Bacterial Communities of Diverse Drosophila Species: Ecological Context of a Host–Microbe Model System

  2011cited by this paper
• Dynamic Gut Microbiome across Life History of the Malaria Mosquito Anopheles gambiae in Kenya

  2011cited by this paper
• Rethinking microbial diversity analysis in the high throughput sequencing era.

  2011cited by this paper
• The emerging role for bacteria in lignin degradation and bio-product formation.

  2011cited by this paper
• Bacterial symbionts in insects or the story of communities affecting communities

  2011cited by this paper
• A simple and distinctive microbiota associated with honey bees and bumble bees

  2011cited by this paper
• The impact of endosymbionts on the evolution of host sex-determination mechanisms.

  2011cited by this paper
• Socially transmitted gut microbiota protect bumble bees against an intestinal parasite

  2011cited by this paper
• Microbiomes of ant castes implicate new microbial roles in the fungus-growing ant Trachymyrmex septentrionalis

  2011cited by this paper
• Army Ants Harbor a Host-Specific Clade of Entomoplasmatales Bacteria

  2010cited by this paper
• QIIME allows analysis of high-throughput community sequencing data

  2010cited by this paper
• Growth and development

  2010cited by this paper
• Geographic Variation in Bacterial Communities Associated With the Red Turpentine Beetle (Coleoptera: Curculionidae)

  2010cited by this paper
• Distribution and Ecology of Frankliniella occidentalis (Thysanoptera: Thripidae) Bacterial Symbionts

  2009cited by this paper
• Bacterial gut symbionts are tightly linked with the evolution of herbivory in ants

  2009cited by this paper
• Symbiotic Nitrogen Fixation in the Fungus Gardens of Leaf-Cutter Ants

  2009cited by this paper
• Transmission of Methylobacterium mesophilicum by Bucephalogonia xanthophis for paratransgenic control strategy of Citrus variegated chlorosis

  2009cited by this paper
• PyNAST: a flexible tool for aligning sequences to a template alignment

  2009cited by this paper
• Localization and transmission route of Coriobacterium glomerans, the endosymbiont of pyrrhocorid bugs.

  2009cited by this paper
• The microbial dimension in insect nutritional ecology

  2009cited by this paper
• The Ecology and Evolution of Microbes that Manipulate Host Reproduction

  2009cited by this paper
• The diversity of reproductive parasites among arthropods: Wolbachia do not walk alone

  2008cited by this paper
• Phylogenetic Characterization of Two Novel Commensal Bacteria Involved with Innate Immune Homeostasis in Drosophila melanogaster

  2008cited by this paper
• First Detection of Wolbachia in Arrhenotokous Populations of Thrips Species (Thysanoptera: Thripidae and Phlaeothripidae) and Its Role in Reproduction

  2008cited by this paper
• The Effects of Hydration on Growth of the House Cricket, Acheta domesticus

  2008cited by this paper
• Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro.

  2008cited by this paper
• Genomics and evolution of heritable bacterial symbionts.

  2008cited by this paper

• TSWV Infection Differentially Reshapes the Symbiotic Microbiome of Two Frankliniella Thrips Species

  2025cites this paper
• Microbiota Anatomical Niche Partitioning of Simulium vanluni (Diptera: Simuliidae)

  2025cites this paper
• Comparative gut microbiome research through the lens of ecology: theoretical considerations and best practices

  2024cites this paper
• Molecular characterization of Haplothrips cerealis Priesner 1939 as a synonym of Haplothrips tritici (Kurdjumov, 1912)

  2024cites this paper
• Detection and Phylogenetic Analysis of Wolbachia in Onion Thrips (Thrips tabaci Lindeman)

  2023influential citation
• Integrative Insight into Relationships between Florivorous Thrips Haplothrips leucanthemi and H. niger (Insecta, Thysanoptera)

  2022cites this paper
• Unraveling bacterial diversity of the Indian Lac Insect Kerria lacca (Kerr) using next generation sequencing

  2022cites this paper
• Variation in gut microbial communities of Chilo suppressalis in the typical bivoltine areas of northern China

  2022cites this paper
• Thrips Microbiome Study in Commercial Avocado (Persea americana Mill.) from Northwest Colombian Andes (Antioquia, Colombia) Shows the Presence of Wolbachia, Ehrlichia, Enterobacter

  2022influential citation
• Characterization of Microbial Communities from the Alimentary Canal of Typhaea stercorea (L.) (Coleoptera: Mycetophagidae)

  2022cites this paper
• Gut bacterial diversity on the basis of feeding behaviour in different species of thrips (Thysanoptera)

  2022influential citation
• Stage correlation of symbiotic bacterial community and function in the development of litchi bugs (Hemiptera: Tessaratomidae)

  2021cites this paper
• High Taxonomic and Functional Diversity of Bacterial Communities Associated with Melon Fly, Zeugodacus cucurbitae (Diptera: Tephritidae)

  2021cites this paper
• Two New Strains of Wolbachia Affecting Natural Avocado Thrips

  2021cites this paper
• Host species identity shapes the diversity and structure of insect microbiota

  2021cites this paper
• Gut microbial composition in developmental stages of gall inducing thrips Gynaikothrips uzeli and associated plant pathogenesis

  2021influential citation
• Bacterial analysis in the early developmental stages of the black tiger shrimp (Penaeus monodon)

  2020cites this paper
• Microbiome-Guided Exploration of the Microbial Assemblage of the Exotic Beverage “Insect Tea” Native to Southwestern China

  2020cites this paper
• Variation in the microbiome of the spider mite Tetranychus truncatus with sex, instar, and endosymbiont infection.

  2020cites this paper
• Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)

  2019influential citation
• The Developmental Stage Symbionts of the Pea Aphid-Feeding Chrysoperla sinica (Tjeder)

  2019cites this paper
• The microbiome profiling of fungivorous black tinder fungus beetle Bolitophagus reticulatus reveals the insight into bacterial communities associated with larvae and adults

  2019cites this paper