Less common applications of monoliths: IV. Recent developments in immobilized enzyme reactors for proteomics and biotechnology.

No abstract is available for this paper.

• Publication year

  2009

• Venue

  Journal of Separation Science

• Publication date

  2009-03-01

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1002/jssc.200800641PMID 19194973PMCID PMC2766777
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity.

  2008cited by this paper
• In-line system containing porous polymer monoliths for protein digestion with immobilized pepsin, peptide preconcentration and nano-liquid chromatography separation coupled to electrospray ionization mass spectroscopy.

  2008cited by this paper
• Development of an integrated chromatographic system for on-line digestion and characterization of phosphorylated proteins.

  2008cited by this paper
• New monolithic enzymatic micro-reactor for the fast production and purification of oligogalacturonides.

  2008cited by this paper
• Development of a silica monolith microbioreactor entrapping highly activated lipase and an experiment toward integration with chromatographic separation of chiral esters.

  2007cited by this paper
• Preparation and characterisation of ribonuclease monolithic bioreactor.

  2007cited by this paper
• Influence of the methacrylate monolith structure on genomic DNA mechanical degradation, enzymes activity and clogging.

  2007cited by this paper
• Chymotrypsin immobilization on epoxy monolithic silica columns: development and characterization of a bioreactor for protein digestion.

  2007cited by this paper
• Characterization of reversible and pseudo-irreversible acetylcholinesterase inhibitors by means of an immobilized enzyme reactor.

  2007cited by this paper
• A bifunctional monolithic column for combined protein preconcentration and digestion for high throughput proteomics research.

  2007cited by this paper
• Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions.

  2007cited by this paper
• Monolith-based immobilized enzyme reactors: recent developments and applications for proteome analysis.

  2007cited by this paper
• Improvement of enzyme activity, stability and selectivity via immobilization techniques

  2007cited by this paper
• Pronase-immobilized enzyme reactor: an approach for automation in glycoprotein analysis by LC/LC-ESI/MSn.

  2007cited by this paper
• CE-microreactor-CE-MS/MS for protein analysis.

  2007cited by this paper
• Entrapment of horseradish peroxidase in sugar-modified silica monoliths: toward the development of a biocatalytic sensor.

  2007cited by this paper
• Development and characterization of beta-secretase monolithic micro-immobilized enzyme reactor for on-line high-performance liquid chromatography studies.

  2007cited by this paper
• Rapid protein digestion and identification using monolithic enzymatic microreactor coupled with nano-liquid chromatography-electrospray ionization mass spectrometry.

  2006cited by this paper
• Less common applications of monoliths: I. Microscale protein mapping with proteolytic enzymes immobilized on monolithic supports

  2006cited by this paper
• Immobilized enzyme reactor chromatography: optimization of protein retention and enzyme activity in monolithic silica stationary phases.

  2006cited by this paper
• Rapid protein identification using monolithic enzymatic microreactor and LC‐ESI‐MS/MS

  2006cited by this paper
• Nanoscale biocatalyst systems.

  2006cited by this paper
• Coupling the immobilized trypsin microreactor of monolithic capillary with muRPLC-MS/MS for shotgun proteome analysis.

  2006cited by this paper
• Optimization of a trypsin-bioreactor coupled with high-performance liquid chromatography-electrospray ionization tandem mass spectrometry for quality control of biotechnological drugs.

  2006cited by this paper
• Immobilization of Enzymes

  2006cited by this paper
• Immobilised enzymes: science or art?

  2005cited by this paper
• Application of immobilized enzyme reactor in on-line high performance liquid chromatography: a review.

  2005cited by this paper
• Azlactone-reactive polymer supports for immobilizing synthetically useful enzymes. II. Important preliminary hydrogen bonding effects in the covalent coupling of Penicillin G Acylase

  2005cited by this paper
• Immobilized trypsin systems coupled on-line to separation methods: recent developments and analytical applications.

  2005cited by this paper
• Characterization of a monolithic immobilized trypsin microreactor with on-line coupling to ESI-MS.

  2005cited by this paper
• Azlactone-reactive polymer supports for immobilizing synthetically useful enzymes: Part I. Pig liver esterase on dispersion polymer supports

  2004cited by this paper
• Enzyme immobilization on epoxy- and 1,1'-carbonyldiimidazole-activated methacrylate-based monoliths.

  2004cited by this paper
• Immobilized microfluidic enzymatic reactors

  2004cited by this paper
• Analytical characterization of a facile porous polymer monolithic trypsin microreactor enabling peptide mass mapping using mass spectrometry.

  2004cited by this paper
• Stable microstructured network for protein patterning on a plastic microfluidic channel: strategy and characterization of on-chip enzyme microreactors.

  2004cited by this paper
• Monolithic materials : preparation, properties and applications

  2003cited by this paper
• Dual-function microanalytical device by in situ photolithographic grafting of porous polymer monolith: integrating solid-phase extraction and enzymatic digestion for peptide mass mapping.

  2003cited by this paper
• Immobilised enzymes: carrier-bound or carrier-free?

  2003cited by this paper
• Photografting and the Control of Surface Chemistry in Three-Dimensional Porous Polymer Monoliths

  2003cited by this paper
• Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices.

  2002cited by this paper
• High-throughput peptide mass mapping using a microdevice containing trypsin immobilized on a porous polymer monolith coupled to MALDI TOF and ESI TOF mass spectrometers.

  2002cited by this paper
• Application of monoliths as supports for affinity chromatography and fast enzymatic conversion.

  2001cited by this paper
• Chemistry and technology of 2‐alkenyl azlactones

  2001cited by this paper
• Immobilization of trypsin onto “molded” macroporous poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) rods and use of the conjugates as bioreactors and for affinity chromatography

  2000cited by this paper
• On-line characterization of the activity and reaction kinetics of immobilized enzyme by high-performance frontal analysis.

  2000cited by this paper
• Design of reactive porous polymer supports for high throughput bioreactors: poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide- co-ethylene dimethacrylate) monoliths.

  1999cited by this paper
• Industrial Applications Of Immobilized Biocatalysts And Biomaterials

  1996cited by this paper
• Continuous rods of macroporous polymer as high-performance liquid chromatography separation media

  1992cited by this paper
• Carrier membrane as a stationary phase for affinity chromatography and kinetic studies of membrane-bound enzymes.

  1991cited by this paper
• High-Performance Membrane Chromatography. A Novel Method of Protein Separation

  1990cited by this paper
• Immobilization of Protein A at high density on azlactone-functional polymeric beads and their use in affinity chromatography

  1990cited by this paper
• Enzyme immobilization techniques on poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) carrier with penicillin amidase as model

  1979cited by this paper
• Methacrylate gels with epoxide groups as supports for immobilization of enzymes in pH range 3-12.

  1978cited by this paper
• Immobilization of amyloglucosidase on poly [(glycidyl methacrylate) Co(ethylene dimethacrylate)] carrier and its derivatives

  1978cited by this paper
• Reactive polymers. XX. The preparation of a carrier with aldehyde groups by oxidation of a macroporous glycidyl methacrylate-ethylenedimethacrylate copolymer with periodic acid†

  1978cited by this paper
• Immobilized coenzymes.

  1976cited by this paper
• Industrial Applications of Immobilized Enzymes and Immobilized Microbial Cells

  1976cited by this paper
• [61] Immobilized coenzymes

  1976cited by this paper
• Reactive polymers I. Macroporous methacrylate copolymers containing epoxy groups

  1975cited by this paper
• Immobilized enzymes for industrial reactors

  1975cited by this paper
• ADSORPTION OF INVERTASE.

  1916cited by this paper

• Development of Liquid Chromatography on Monolithic Supports—From First Concepts to Real Analytical and Preparative Techniques

  2025cites this paper
• Valorization strategies for hazardous proteinaceous waste from rendering production - Recent advances in specified risk materials (SRMs) conversion.

  2023cites this paper
• 3D-Printed High-Pressure-Resistant Immobilized Enzyme Microreactor (μIMER) for Protein Analysis

  2022cites this paper
• A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values.

  2022cites this paper
• Sintered Glass Monoliths as Supports for Affinity Columns

  2021cites this paper
• Monolithic flow reactor for enzymatic oxidations

  2021cites this paper
• Modulation of polymer-based immobilized enzymes for industrial scale applications

  2021cites this paper
• Open Porous Composite Monoliths for Biomedical Applications via Photocrosslinking of Low Internal Phase Nano-Emulsion Templates

  2021cites this paper
• Sintered Glass Monoliths as New Supports for Affinity Columns

  2021cites this paper
• Genetically Programmed Regioselective Immobilization of Enzymes in Biosilica Microparticles

  2020cites this paper
• Bioconversion of specified risk materials-derived peptides for tackifier and torrefied wood binder applications

  2020cites this paper
• Flow‐through enzymatic reactors using polymer monoliths: From motivation to application

  2020cites this paper
• Investigating Monoliths (Vinyl Azlactone-co-Ethylene Dimethacrylate) as a Support for Enzymes and Drugs, for Proteomics and Drug-Target Studies

  2019cites this paper
• Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

  2019cites this paper
• Current trends in affinity-based monoliths in microextraction approaches: A review.

  2019cites this paper
• Detection of halogenated hydrocarbon pollutants using enzymatic reflectance biosensor

  2019cites this paper
• Thiol-ene based microfluidic devices for bioanalysis

  2019cites this paper
• Design and Functionalization of Alumina Monoliths for Protein Purification by Chromatography

  2019cites this paper
• Immobilized enzyme‐based analytical tools in the ‐omics era: Recent advances

  2018cites this paper
• Selective Fishing for Peptides with Antibody-Immobilized Acrylate Monoliths, Coupled Online with NanoLC-MS.

  2018cites this paper
• Online entrapment of epitope-containing peptides from integrins using immobilized antibodies

  2018cites this paper
• On-a-chip tryptic digestion of transthyretin: a step toward an integrated microfluidic system for the follow-up of familial transthyretin amyloidosis.

  2018cites this paper
• High-Resolution Nano-Liquid Chromatography with Tandem Mass Spectrometric Detection for the Bottom-Up Analysis of Complex Proteomic Samples

  2018cites this paper
• Immobilized-enzyme reactors integrated with capillary electrophoresis for pharmaceutical research.

  2018cites this paper
• Highly stable adsorptive and covalent immobilization of Thermomyces lanuginosus lipase on tailor-made porous carbon material

  2018cites this paper
• Microfluidic reactors with immobilized enzymes—Characterization, dividing, perspectives

  2017cites this paper
• Macroporous monoliths for biodegradation study of polymer particles considered as drug delivery systems

  2017cites this paper
• A lab-on-a-chip for monolith-based preconcentration and electrophoresis separation of phosphopeptides.

  2017cites this paper
• A Simple Approach to Enhance the Water Stability of a Metal-Organic Framework.

  2017cites this paper
• Preparation and applications of monolithic structures containing metal-organic frameworks.

  2017cites this paper
• Advances in organic polymer-based monolithic column technology for high-resolution liquid chromatography-mass spectrometry profiling of antibodies, intact proteins, oligonucleotides, and peptides.

  2017cites this paper
• Multichannel Open Tubular Enzyme Reactor Online Coupled with Mass Spectrometry for Detecting Ricin.

  2017cites this paper
• Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

  2017cites this paper
• Functionalized acetoacetylated poly(vinyl alcohol) monoliths for enzyme immobilization: a phase separation method

  2017cites this paper
• Immobilized enzyme reactors based on monoliths: Effect of pore size and enzyme loading on biocatalytic process

  2017cites this paper
• Towards automation in protein digestion: Development of a monolithic trypsin immobilized reactor for highly efficient on-line digestion and analysis.

  2017cites this paper
• Monoliths in capillary electrochromatography and capillary liquid chromatography in conjunction with mass spectrometry

  2016cites this paper
• Fluorescence Microscopy Imaging of an Immobilized Enzyme Microreactor to Investigate Glutaraldehyde-Mediated Crosslinking of Chymotrypsin

  2016cites this paper
• In-situ sol-gel modification strategies to develop a monolith continuous microreactor for enzymatic green reactions

  2016cites this paper
• Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization.

  2016cites this paper
• Preparation and evaluation of dual-enzyme microreactor with co-immobilized trypsin and chymotrypsin.

  2016cites this paper
• Characterization of glutaraldehyde-immobilized chymotrypsin and an in-situ immobilized enzyme reactor using capillary electrophoresis-based peptide mapping

  2016cites this paper
• Physisorption of α-chymotrypsin on SiO2 and TiO2: A comparative study via experiments and molecular dynamics simulations.

  2016cites this paper
• Layer-by-Layer Assembly of Metal-Organic Frameworks in Macroporous Polymer Monolith and Their Use for Enzyme Immobilization.

  2016cites this paper
• Capillary electrophoresis-integrated immobilized enzyme reactors

  2016cites this paper
• Développement d’un laboratoire sur puce pour la préconcentration sur support monolithique. Application à l'enrichissement et la séparation en ligne de phosphopeptides.

  2016cites this paper
• A one-step preparation method of monolithic enzyme reactor for highly efficient sample preparation coupled to mass spectrometry-based proteomics studies.

  2015cites this paper
• Fabrication of an on-line enzyme micro-reactor coupled to liquid chromatography-tandem mass spectrometry for the digestion of recombinant human erythropoietin.

  2015cites this paper
• Recent Advances and Uses of Monolithic Columns for the Analysis of Residues and Contaminants in Food

  2015cites this paper
• Xylan degradation improved by a combination of monolithic columns bearing immobilized recombinant β‐xylosidase from Aspergillus awamori X‐100 and Grindamyl H121 β‐xylanase

  2015cites this paper
• Physisorption of enzymatically active chymotrypsin on titania colloidal particles.

  2015cites this paper
• Investigation of bi-enzymatic reactor based on hybrid monolith with nanoparticles embedded and its proteolytic characteristics.

  2015cites this paper
• Fabrication of a polystyrene microfluidic chip coupled to electrospray ionization mass spectrometry for protein analysis.

  2015cites this paper
• Separation and identification of oligomeric ethyl silicates by liquid chromatography with electrospray ionization mass spectrometry.

  2015cites this paper
• A novel polymeric monolith prepared with multi-acrylate crosslinker for retention-independent efficient separation of small molecules in capillary liquid chromatography.

  2015cites this paper
• Post-polymerization Modification of Surface-Bound Polymers

  2015cites this paper
• A new strategy for simultaneous synthesis and efficient anchorage of polymer monoliths in native PDMS microchips

  2015cites this paper
• Preparation of a novel polymer monolith with functional polymer brushes by two-step atom-transfer radical polymerization for trypsin immobilization.

  2014cites this paper
• Materials‐based strategies for multi‐enzyme immobilization and co‐localization: A review

  2014cites this paper
• Patenting trends in enzyme related microfluidic applications

  2014cites this paper
• Mucin-based stationary phases as tool for the characterization of drug-mucus interaction.

  2014cites this paper
• Microscale immobilized enzyme reactors in proteomics: latest developments.

  2014cites this paper
• Hybrid organic-inorganic monolithic enzymatic reactor with SBA-15 nanoparticles incorporated.

  2014cites this paper
• Immobilized antibody on a hybrid organic-inorganic monolith: Capillary immunoextraction coupled on-line to nanoLC-UV for the analysis of microcystin-LR.

  2013cites this paper
• Flow-through immobilized enzyme reactors based on monoliths: II. Kinetics study and application.

  2013cites this paper
• Silica-based monolithic capillary columns modified by liposomes for characterization of analyte-liposome interactions by capillary liquid chromatography.

  2013cites this paper
• Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

  2013cites this paper
• Integrated enzyme reactor and high resolving chromatography in “sub-chip” dimensions for sensitive protein mass spectrometry

  2013cites this paper
• Monolithic columns in plant proteomics and metabolomics

  2013cites this paper
• Cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatography.

  2013cites this paper
• Screening α-glucosidase inhibitor from natural products by capillary electrophoresis with immobilised enzyme onto polymer monolith modified by gold nanoparticles.

  2013cites this paper
• Rapid separations by LC using ion-exchange media based on spongy monoliths.

  2013cites this paper
• Human recombinant beta-secretase immobilized enzyme reactor for fast hits' selection and characterization from a virtual screening library.

  2013cites this paper
• High-resolution monolithic columns—a new tool for effective and quick separation

  2013cites this paper
• Rapid and Efficient Proteolysis for Protein Analysis by an Aptamer-Based Immobilized Chymotrypsin Microreactor

  2013cites this paper
• Biocatalytic reactors based on ribonuclease A immobilized on macroporous monolithic supports

  2013cites this paper
• Monolithic polymeric sorbents for high-performance chromatography of synthetic polymers

  2013cites this paper
• An aptamer-based trypsin reactor for on-line protein digestion with electrospray ionization tandem mass spectrometry.

  2013cites this paper
• Investigation of carryover of peptides in nano-liquid chromatography/mass spectrometry using packed and monolithic capillary columns.

  2013cites this paper
• Flow-through immobilized enzyme reactors based on monoliths: I. Preparation of heterogeneous biocatalysts.

  2013cites this paper
• Analytical silica monoliths with submicron macropores: current limitations to a direct morphology-column efficiency scaling.

  2013cites this paper
• Amino-functionalized monolithic spin-type columns for high-throughput lectin affinity chromatography of glycoproteins.

  2012cites this paper
• Less common applications of monoliths: V. Monolithic scaffolds modified with nanostructures for chromatographic separations and tissue engineering.

  2012cites this paper
• New monolithic chromatographic supports for macromolecules immobilization: challenges and opportunities.

  2012cites this paper
• Emerging Technologies for Decentralized Production of PET Tracers

  2012cites this paper
• Immobilization of Chymotrypsin on Silica Beads Based on High Affinity and Specificity Aptamer and Its Applications

  2012cites this paper
• Recent Developments of Extraction and Micro-extraction Technologies with Porous Monoliths

  2012cites this paper
• A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters

  2012cites this paper
• Bioconjugation of trypsin onto gold nanoparticles: effect of surface chemistry on bioactivity.

  2012cites this paper
• Recent developments and future possibilities for polymer monoliths in separation science.

  2012cites this paper
• Purification, Characterization And Immobilization Of Lignin Peroxidase Produced By Ganoderma Lucidum For Industrial Applications

  2012cites this paper
• Application of a Burkholderia cepacia lipase-immobilized silica monolith micro-bioreactor to continuous-flow kinetic resolution for transesterification of (R, S)-1-phenylethanol

  2012cites this paper
• A hydrophilic immobilized trypsin reactor with N-vinyl-2-pyrrolidinone modified polymer microparticles as matrix for highly efficient protein digestion with low peptide residue.

  2012cites this paper
• Polymeric monolithic materials modified with nanoparticles for separation and detection of biomolecules: A review

  2012cites this paper
• Monolithic media for applications in affinity chromatography.

  2011cites this paper
• Silica-based and organic monolithic capillary columns for LC: recent trends in proteomics.

  2011cites this paper
• Miniaturization in biotechnology: speeding up the development of bioprocesses.

  2011cites this paper
• Effects of functional monomers on retention behavior of small and large molecules in monolithic capillary columns at isocratic and gradient conditions.

  2011cites this paper
• Photopatterned materials in bioanalytical microfluidic technology

  2011cites this paper
• Applications of Magnetic Resonance to Current Detection and Microscale Flow Imaging

  2011cites this paper