Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing

Tibetan Chickens should have unique gastrointestinal microbiota because of their particular habitats. Thus, the aim of this study was to investigate the cecal microbiota of Tibetan Chickens from five typical high‐altitude regions of China. Lohmann egg‐laying hens (LMs) and Daheng broiler chickens (DHs) were chosen as controls. The cecal bacterial populations of Tibetan Chickens were surveyed by high‐throughput sequencing (HTS) of the bacterial 16S rRNA hypervariable region V3‐V4 (16S rRNAV3‐V4) combined with community‐fingerprinting analysis of the 16S rRNA gene based on polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE). The results revealed that the majority of cecal microbiota differed between the Tibetan Chicken and LM/DH. The microbial communities in the cecum were composed of 16 phyla, 28 classes, 36 orders, 57 families, 101 genera, and 189 species. Represented phyla were Bacteroidetes (>47%), Firmicutes (>18.8%), Spirochaetae (>0.3%), and Proteobacteria (>0.4%). Bacteroides and the RC9 gut group were the two most abundant genera. There were relatively more Christensenellaceae, Subdoligranulum, Spirochaeta, and Treponema in Tibetan Chickens, whereas there were more Phascolarctobacterium, Faecalibacterium, Megamonas, and Desulfovibrio in LMs and DHs. The cecal microbiota of Tibetan Chicken have slightly diverged due to exposure to different geographic environments. Differences in the intestinal bacterial communities of Tibetan Chicken and LM/DH were noted.

• Publication year

  2016

• Venue

  MicrobiologyOpen

• Publication date

  2016-05-02

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology, Environmental Science

• Identifiers
  DOI 10.1002/mbo3.367PMID 27139888PMCID 5061713
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2016cited by this paper
• Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta.

  2015cited by this paper
• A comparative study on microbiota from the intestine of Prussian carp (Carassius gibelio) and their aquatic environmental compartments, using different molecular methods

  2015cited by this paper
• Mucosa-Associated Faecalibacterium prausnitzii Phylotype Richness Is Reduced in Patients with Inflammatory Bowel Disease

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  2015cited by this paper
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  2014cited by this paper
• Bacteria, phages and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations

  2014cited by this paper
• Gut Microbiota Elicits a Protective Immune Response against Malaria Transmission

  2014cited by this paper
• Human genetics shape the gut microbiome.

  2014influential reference
• Correlations between colonic crypt mucin chemotype, inflammatory grade and Desulfovibrio species in ulcerative colitis

  2014cited by this paper
• Microbiota of the chicken gastrointestinal tract: influence on health, productivity and disease

  2014cited by this paper
• Analysis of artifacts suggests DGGE should not be used for quantitative diversity analysis.

  2013cited by this paper
• Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes

  2013cited by this paper
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  2013cited by this paper
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  2012cited by this paper
• Next Generation Sequencing to Define Prokaryotic and Fungal Diversity in the Bovine Rumen

  2012cited by this paper
• Description of Christensenella minuta gen. nov., sp. nov., isolated from human faeces, which forms a distinct branch in the order Clostridiales, and proposal of Christensenellaceae fam. nov.

  2012cited by this paper
• Characterization of Phascolarctobacterium succinatutens sp. nov., an Asaccharolytic, Succinate-Utilizing Bacterium Isolated from Human Feces

  2011cited by this paper
• Comparative fecal metagenomics unveils unique functional capacity of the swine gut

  2011cited by this paper
• Exposure of different bacterial inocula to newborn chicken affects gut microbiota development and ileum gene expression

  2010cited by this paper
• The phylogeography of Adelie penguin faecal flora.

  2009cited by this paper
• Megamonas rupellensis sp. nov., an anaerobe isolated from the caecum of a duck.

  2008cited by this paper
• Cloacal microbial communities of female spotted towhees Pipilo maculatus: microgeographic variation and individual sources of variability

  2008cited by this paper
• Comparison of the Cecal Microbiota of Domestic and Wild Turkeys

  2008cited by this paper
• Interactions between commensal bacteria and the gut-associated immune system of the chicken

  2008cited by this paper
• Degradation of trichothecene mycotoxins by chicken intestinal microbes.

  2007cited by this paper
• Measuring Species Richness Based on Microbial Community Fingerprints: the Emperor Has No Clothes

  2007cited by this paper
• Phylogenetic analysis of intestinal bacteria in the Chinese mitten crab (Eriocheir sinensis)

  2007cited by this paper
• Appropriate chicken sample size for identifying the composition of broiler intestinal microbiota affected by dietary antibiotics, using the polymerase chain reaction-denaturing gradient gel electrophoresis technique.

  2007cited by this paper
• Quantitative analysis of the intestinal bacterial community in one‐ to three‐week‐old commercially reared broiler chickens fed conventional or antibiotic‐free vegetable‐based diets

  2006cited by this paper
• Maximum growth rates and possible life strategies of different bacterioplankton groups in relation to phosphorus availability in a freshwater reservoir.

  2006cited by this paper
• Molecular ecological analysis of the gastrointestinal microbiota: a review.

  2004cited by this paper
• GC Fractionation Enhances Microbial Community Diversity Assessment and Detection of Minority Populations of Bacteria by Denaturing Gradient Gel Electrophoresis

  2004cited by this paper
• Characteristics of the gastrointestinal microbial communities, with special reference to the chicken

  2004cited by this paper
• Subdoligranulum variabile gen. nov., sp. nov. from human feces.

  2004cited by this paper
• Diversity and Succession of the Intestinal Bacterial Community of the Maturing Broiler Chicken

  2003cited by this paper
• 16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens

  2002cited by this paper
• Quantifying biodiversity: procedures and pitfalls in the measurement and comparison of species richness

  2001cited by this paper
• DGGE/TGGE a method for identifying genes from natural ecosystems.

  1999cited by this paper
• Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

  1993influential reference
• Microbes of the avian cecum: types present and substrates utilized.

  1989cited by this paper
• The avian intestinal flora with particular reference to the possible ecological significance of the cecal anaerobic bacteria.

  1972cited by this paper

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  2026cites this paper
• Collaboration of the symbiotic microbiome and host genome during the high altitude adaptation of chickens.

  2026cites this paper
• Composition of microbiota from chicken caecal mucosa considerably differs from microbiota in the caecal lumen.

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• Multi‐omics analysis of gut‐organ axes reveals the high‐altitude adaptation in Tibetan chicken

  2025cites this paper
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  2024cites this paper
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• Coping with extremes: Alternations in diet, gut microbiota, and hepatic metabolic functions in a highland passerine.

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• Microbiota, Gut Health and Chicken Productivity: What Is the Connection?

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  2019cites this paper
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