Nerve-independent formation of a topologically complex postsynaptic apparatus

As the mammalian neuromuscular junction matures, its acetylcholine receptor (AChR)–rich postsynaptic apparatus is transformed from an oval plaque into a pretzel-shaped array of branches that precisely mirrors the branching pattern of the motor nerve terminal. Although the nerve has been believed to direct postsynaptic maturation, we report here that myotubes cultured aneurally on matrix-coated substrates form elaborately branched AChR-rich domains remarkably similar to those seen in vivo. These domains share several characteristics with the mature postsynaptic apparatus, including colocalization of multiple postsynaptic markers, clustering of subjacent myonuclei, and dependence on the muscle-specific kinase and rapsyn for their formation. Time-lapse imaging showed that branched structures arise from plaques by formation and fusion of AChR-poor perforations through a series of steps mirroring that seen in vivo. Multiple fluorophore imaging showed that growth occurs by circumferential, asymmetric addition of AChRs. Analysis in vivo revealed similar patterns of AChR addition during normal development. These results reveal the sequence of steps by which a topologically complex domain forms on a cell and suggest an unexpected nerve-independent role for the postsynaptic cell in generating this topological complexity.

• Publication year

  2004

• Venue

  Journal of Cell Biology

• Publication date

  2004-03-29

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.200401115PMID 15037598PMCID 2172060
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• In vivo development shows similar patterns of AChR addition, supporting a shared mechanism for topological remodeling of the postsynaptic apparatus.

  Confidence 0.90

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• Branched structures arise from plaque-like AChR accumulations through the formation and fusion of AChR-poor perforations, and their growth proceeds by circumferential, asymmetric AChR addition.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• Formation of the branched AChR-rich domains depends on muscle-specific kinase and rapsyn and is accompanied by colocalization of postsynaptic markers and clustering of subjacent myonuclei.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• Aneurally cultured myotubes on matrix-coated substrates can form elaborately branched AChR-rich domains resembling the mature neuromuscular junction postsynaptic apparatus seen in vivo.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review

• achr-poor perforations

  cellular feature

  Low-receptor holes or openings that appear within an AChR plaque during morphological remodeling.

  Aliases: acetylcholine receptor-poor perforations

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• achr-rich postsynaptic apparatus

  cellular structure

  The specialized postsynaptic membrane domain at the neuromuscular junction that is enriched in acetylcholine receptors.

  Aliases: postsynaptic apparatus

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• branched achr-rich domains

  cellular structure

  Complex acetylcholine receptor-rich membrane domains with branch-like morphology formed by cultured myotubes in this system.

  Aliases: branched domains

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• circumferential, asymmetric achr addition

  process

  A spatial pattern of receptor incorporation around the perimeter of a domain with unequal addition across the structure.

  Aliases: asymmetric AChR addition

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• matrix-coated substrates

  material

  Culture surfaces coated with extracellular matrix material used to grow myotubes in the aneurally cultured condition.

  Aliases: matrix-coated culture substrates

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• muscle-specific kinase

  protein

  A muscle receptor tyrosine kinase involved in organizing postsynaptic specialization at the neuromuscular junction.

  Aliases: MuSK

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review
• rapsyn

  protein

  A postsynaptic scaffold protein that links acetylcholine receptors to the neuromuscular junction cytoskeleton and clustering machinery.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewKiller Whale (322360f1c1) review뀨 (7c402c1b98) review

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