A heterozygous moth genome provides insights into herbivory and detoxification

How an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants, but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood. We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management.

• Publication year

  2013

• Venue

  Nature Genetics

• Publication date

  2013-01-13

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/ng.2524PMID 23313953
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Temperature treatments during larval development reveal extensive heritable and plastic variation in gene expression and life history traits

  2013cited by this paper
• Diamondback moth ecology and management: problems, progress, and prospects.

  2013cited by this paper
• Specificity of plant – enemy interactions Specialist versus generalist insect herbivores and plant defense

  2012cited by this paper
• Estimating the Economic Cost of One of the World's Major Insect Pests, Plutella xylostella (Lepidoptera: Plutellidae): Just How Long is a Piece of String?

  2012cited by this paper
• Butterfly genome reveals promiscuous exchange of mimicry adaptations among species

  2012cited by this paper
• Developmental and insecticide-resistant insights from the de novo assembled transcriptome of the diamondback moth, Plutella xylostella.

  2012cited by this paper
• Gossypol‐enhanced P450 gene pool contributes to cotton bollworm tolerance to a pyrethroid insecticide

  2012cited by this paper
• Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance

  2011cited by this paper
• Adaptive seeds tame genomic sequence comparison.

  2011cited by this paper
• The monarch butterfly genome yields insights into long-distance migration.

  2011cited by this paper
• The Arabidopsis lyrata genome sequence and the basis of rapid genome size change

  2011cited by this paper
• Linkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism

  2011cited by this paper
• The genome of Tetranychus urticae reveals herbivorous pest adaptations

  2011cited by this paper
• The genome of the mesopolyploid crop species Brassica rapa

  2011cited by this paper
• An Archaeopteryx-like theropod from China and the origin of Avialae

  2011cited by this paper
• Genome sequence and analysis of the tuber crop potato

  2011cited by this paper
• Supporting Online Material

  2011cited by this paper
• Genetic analysis of the xenobiotic resistance‐associated ABC gene subfamilies of the Lepidoptera

  2011cited by this paper
• Complex inheritance of larval adaptation in Plutella xylostella to a novel host plant

  2011cited by this paper
• Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects.

  2011cited by this paper
• Parallel Evolution of Bacillus thuringiensis Toxin Resistance in Lepidoptera

  2011cited by this paper
• Stampy: a statistical algorithm for sensitive and fast mapping of Illumina sequence reads.

  2011cited by this paper
• Multipoint maximum likelihood mapping in a full-sib family of an outbreeding species.

  2011cited by this paper
• Genomic Comparison of the Ants Camponotus floridanus and Harpegnathos saltator

  2010cited by this paper
• Survey of a Salivary Effector in Caterpillars: Glucose Oxidase Variation and Correlation with Host Range

  2010cited by this paper
• Genome-Enabled Research on the Ecology of Plant-Insect Interactions

  2010cited by this paper
• Comprehensive gene and taxon coverage elucidates radiation patterns in moths and butterflies

  2010cited by this paper
• Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle

  2010cited by this paper
• CDD: a Conserved Domain Database for the functional annotation of proteins

  2010cited by this paper
• Sulfatase activities towards the regulation of cell metabolism and signaling in mammals

  2010cited by this paper
• The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.

  2010cited by this paper
• Functional and Evolutionary Insights from the Genomes of Three Parasitoid Nasonia Species

  2010cited by this paper
• Genome Sequence of the Pea Aphid Acyrthosiphon pisum

  2010cited by this paper
• The sequence and de novo assembly of the giant panda genome

  2010cited by this paper
• Mis-Spliced Transcripts of Nicotinic Acetylcholine Receptor α6 Are Associated with Field Evolved Spinosad Resistance in Plutella xylostella (L.)

  2010cited by this paper
• Infernal 1.0: inference of RNA alignments

  2009cited by this paper
• Spontaneous mapping of number and space in adults and young children.

  2009cited by this paper
• SOAP2: an improved ultrafast tool for short read alignment

  2009cited by this paper
• SNP detection for massively parallel whole-genome resequencing.

  2009cited by this paper
• TopHat: discovering splice junctions with RNA-Seq

  2009cited by this paper
• SNP detection for massively parallel whole-genome resequencing

  2009cited by this paper
• Toward reconstructing the evolution of advanced moths and butterflies (Lepidoptera: Ditrysia): an initial molecular study

  2009cited by this paper
• Mapping and quantifying mammalian transcriptomes by RNA-Seq

  2008cited by this paper
• SOAP: short oligonucleotide alignment program

  2008cited by this paper
• Synteny and Collinearity in Plant Genomes

  2008cited by this paper
• Mapping the larval midgut lumen proteome of Helicoverpa armigera, a generalist herbivorous insect.

  2008cited by this paper
• The genome of the model beetle and pest Tribolium castaneum

  2008cited by this paper
• The gustatory receptor family in the silkworm moth Bombyx mori is characterized by a large expansion of a single lineage of putative bitter receptors

  2008cited by this paper
• InterPro: the integrative protein signature database

  2008cited by this paper
• Dating the early evolution of plants: detection and molecular clock analyses of orthologs

  2007cited by this paper
• Creating a honey bee consensus gene set

  2007cited by this paper
• PAML 4: phylogenetic analysis by maximum likelihood.

  2007cited by this paper
• Transposable elements are enriched within or in close proximity to xenobiotic-metabolizing cytochrome P450 genes

  2007cited by this paper
• Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics.

  2007cited by this paper
• LTR_FINDER: an efficient tool for the prediction of full-length LTR retrotransposons

  2007cited by this paper
• Insights into social insects from the genome of the honeybee Apis mellifera

  2006cited by this paper
• Investigation of Resistance Mechanisms to Fipronil in Diamondback Moth (Lepidoptera: Plutellidae)

  2006cited by this paper
• Erratum: Insights into social insects from the genome of the honeybee Apis mellifera

  2006cited by this paper
• Insights into social insects from the genome of the honeybee Apis mellifera

  2006cited by this paper
• Sulfur metabolism: a versatile platform for launching defence operations.

  2005cited by this paper
• AUGUSTUS: a web server for gene prediction in eukaryotes that allows user-defined constraints

  2005cited by this paper
• Repbase Update, a database of eukaryotic repetitive elements

  2005cited by this paper
• TreeFam: a curated database of phylogenetic trees of animal gene families

  2005cited by this paper
• De novo identification of repeat families in large genomes

  2005cited by this paper
• SOAP-based services provided by the European Bioinformatics Institute

  2005cited by this paper
• InterProScan: protein domains identifier

  2005cited by this paper
• PILER: identification and classification of genomic repeats

  2005cited by this paper
• A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori)

  2004cited by this paper
• GeneWise and Genomewise.

  2004cited by this paper
• UniProt: the Universal Protein knowledgebase

  2004cited by this paper
• Gene finding in novel genomes

  2004cited by this paper
• The European ribosomal RNA database

  2004cited by this paper
• MUSCLE: multiple sequence alignment with high accuracy and high throughput.

  2004cited by this paper
• Rfam: an RNA family database

  2003cited by this paper
• LTR_STRUC: a novel search and identification program for LTR retrotransposons

  2003cited by this paper
• The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases.

  2003cited by this paper
• Disarming the mustard oil bomb

  2002cited by this paper
• BLAT--the BLAST-like alignment tool.

  2002cited by this paper
• The Genome Sequence of the Malaria Mosquito Anopheles gambiae

  2002cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• KEGG: Kyoto Encyclopedia of Genes and Genomes

  2000cited by this paper
• The genome sequence of Drosophila melanogaster.

  2000cited by this paper
• EMBOSS: the European Molecular Biology Open Software Suite.

  2000cited by this paper
• Genetic mapping of resistance to Bacillus thuringiensis toxins in diamondback moth using biphasic linkage analysis.

  1999cited by this paper
• Tandem repeats finder: a program to analyze DNA sequences.

  1999cited by this paper
• KEGG: Kyoto Encyclopedia of Genes and Genomes

  1999cited by this paper
• tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

  1997cited by this paper
• Prediction of complete gene structures in human genomic DNA.

  1997cited by this paper
• Biology, Ecology, and Management of the Diamondback Moth

  1993cited by this paper
• DDT-Resistance in Plutella maculipennis (Curt.) (Lep.) in Java.

  1953cited by this paper
• BIOINFORMATICS APPLICATIONS

  year unknowncited by this paper
• Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method

  year unknowncited by this paper
• Defining 3D residue environment in protein structures using SCORPION and FORMIGA

  year unknowncited by this paper

• Temporal dynamics of insecticide-resistance mutations in the diamondback moth Plutella xylostella (Linnaeus, 1758) within northern immigration regions

  2026cites this paper
• A cuticular protein mediates thermal and desiccation tolerance through dual regulation of barrier function and water homeostasis.

  2026cites this paper
• Caenorhabditis elegans: A Model System for Accelerating Studies on Bacillus thuringiensis Infection in Insects.

  2026cites this paper
• From Field Overexpression to Biochemical Function: PxCYP6B4-Mediated Hydroxylation Confers Pyrethroid Resistance in Plutella xylostella.

  2026cites this paper
• Forage quality shapes physiological and gut microbial responses in moose (Alces alces) of Isle Royale National Park

  2026cites this paper
• Convergent Evolution and Predictability of Gene Copy Numbers Associated with Diets in Mammals

  2025cites this paper
• ABCC3 mediates insecticide metabolism and functions as a receptor for Bt toxins in Plutella xylostella.

  2025cites this paper
• An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking

  2025cites this paper
• Insect Oral Secretion Protein and Its Related Core Peptide Induce the Host Plant's Endogenous Abscisic Acid to Enhance Resistance against Insect.

  2025cites this paper
• Chromosome level genome assembly of tea tussock moth, Euproctis pseudoconspersa

  2025influential citation
• Effects of drainage and long-term tillage on greenhouse gas fluxes in a natural wetland: insights from microbial mechanisms

  2025cites this paper
• Deorphanization of Pheromone Receptors and Discovery of a Novel Agonist for Sex Pheromone Communication in Diamondback Moths.

  2025cites this paper
• Telomere-to-telomere genome assembly of the Dipteran Bactrocera dorsalis from a single individual

  2025cites this paper
• Reverse engineering high-level resistance to Bt Cry1Ac toxin in Plutella xylostella reveals a hormonal regulatory feedback pathway.

  2025cites this paper
• Transcriptome analysis and temporal expression patterns of wing development-related genes in Lymantria dispar (Lepidoptera: Erebidae).

  2025cites this paper
• HSC70-3 in the Gut Regurgitant of Diamondback Moth, Plutella xylostella: A Candidate Effector for Host Plant Adaptation

  2025cites this paper
• Single-Cell Transcriptomic Profiling Reveals Diet-Dependent Dynamics of Glucosinolate Sulfatases Expression and Cellular Origin in the Midgut of Plutella xylostella.

  2025cites this paper
• Dynamic changes and functional analysis of gut bacteria in Plutella xylostella under different physiological conditions

  2025cites this paper
• Functional Role of the PxGRHPR2 Gene in the Host Plant Adaptation of Diamondback Moth (Plutella xylostella).

  2025cites this paper
• Inhibition of Glucosinolate Sulfatases to Combat Plutella xylostella: Development of Novel Phenylcarboxamide Derivatives for Plant-Integrated Pest Management.

  2025cites this paper
• A Token Stimulus Receptor Tuned to Gluconapin in the Cabbage White Butterfly.

  2025cites this paper
• Mating-induced DNA replication factor minichromosome maintenance protein 10 promotes oogenesis in Plutella xylostella

  2025cites this paper
• Dual interference with host neuropeptide signaling allows parasitoid wasp to hijack host sugar metabolism.

  2025cites this paper
• Transcriptional response in larvae of the generalist fruit fly Anastrepha obliqua feeding on three different tropical host plants

  2025cites this paper
• CRISPR/Cas9 Applications in Functional Genomics of Lepidopteran Pests

  2025cites this paper
• The T2T Genome of the Domesticated Silkworm Bombyx mori

  2024cites this paper
• Functional analysis of dopa decarboxylase in the larval pupation and immunity of the diamondback moth, Plutella xylostella.

  2024cites this paper
• Mode of inheritance for pesticide resistance, importance and prevalence: A review.

  2024cites this paper
• An Orphan Gene Enhances Male Reproductive Success in Plutella xylostella

  2024cites this paper
• Adaptation During the Shift from Entomopathogen to Endosymbiont Is Accompanied by Gene Loss and Intensified Selection

  2024cites this paper
• The ATP-binding cassette transporter subfamily G member 4 mediates cuticular hydrocarbon transport to regulate drought tolerance in Acyrthosiphon pisum.

  2024cites this paper
• Comparative transcriptome analysis provides comprehensive insight into the molecular mechanisms of heat adaption in Plutella xylostella.

  2024cites this paper
• Physiological and Molecular Mechanisms of Lepidopteran Insects: Genomic Insights and Applications of Genome Editing for Future Research

  2024cites this paper
• Nap1 is essential for eupyrene spermatogenesis and migration in Plutella xylostella.

  2024cites this paper
• Functional Role of Odorant-Binding Proteins in Response to Sex Pheromone Component Z8-14:Ac in Grapholita molesta (Busck)

  2024cites this paper
• Bacteria Derived from Diamondback Moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), Gut Regurgitant Negatively Regulate Glucose Oxidase-Mediated Anti-Defense Against Host Plant

  2024cites this paper
• Genomic identification and evolutionary analysis of chemosensory receptor gene families in two Phthorimaea pest species: insights into chemical ecology and host adaptation

  2024cites this paper
• CRISPR/Cas9-Based Functional Characterization of SfUGT50A15 Reveals Its Roles in the Resistance of Spodoptera frugiperda to Chlorantraniliprole, Emamectin Benzoate, and Benzoxazinoids

  2024cites this paper
• Gustatory Receptor 206 Participates in the Foraging Behavior of Larvae of Polyphagous Pest Spodoptera litura.

  2024cites this paper
• Squeeze pumping of lipids and insecticides by ABCH transporter.

  2024cites this paper
• Functions of the sulfatase‐modifying factor 1 (HaSumf1) in the development and host glucosinolate adaptation of Helicoverpa armigera

  2024cites this paper
• Exploring the role of ovary-serine protease gene in female fertility of diamondback moth using CRISPR/Cas9.

  2024cites this paper
• Plasticity in moth mating behavior and ejaculate proteomic diversity induced by male competition.

  2024cites this paper
• The chromosome-level genome assembly of the red swamp crayfish Procambarus clarkii

  2024cites this paper
• Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences

  2024cites this paper
• Identification and Preliminary Characterization of Odorant-Binding Proteins in Neoceratitis asiatica (Diptera: Tephritidae)

  2024cites this paper
• Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest.

  2024cites this paper
• Functions and regulations of insect gut bacteria

  2024cites this paper
• Polygenic adaptation of a cosmopolitan pest to a novel thermal environment

  2024cites this paper
• Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth, Plutella xylostella

  2024cites this paper
• ABCH2 transporter mediates deltamethrin uptake and toxicity in the malaria vector Anopheles coluzzii

  2023cites this paper
• The Potential Role of the Piwi Gene in the Development and Reproduction of Plutella xylostella

  2023cites this paper
• A detoxification pathway initiated by a nuclear receptor TcHR96h in Tetranychus cinnabarinus (Boisduval)

  2023cites this paper
• New Insights into the Plutella xylostella Detoxifying Enzymes: Sequence Evolution, Structural Similarity, Functional Diversity, and Application Prospects of Glucosinolate Sulfatases

  2023cites this paper
• Development of reverse genetics tools in the two-spotted spider mite Tetranychus urticae

  2023cites this paper
• Identification and expression profiles of candidate chemoreceptor genes in the tea leafhopper, Empoasca onukii Matsuda (Hemiptera: Cicadellidae)

  2023cites this paper
• Profiling of Dermanyssus gallinae genes involved in acaricide resistance.

  2023cites this paper
• Tracking Adaptive Pathways of Invasive Insects: Novel Insight from Genomics

  2023cites this paper
• Population Genomics Provide Insights into the Evolution and Adaptation of the Asia Corn Borer

  2023cites this paper
• Genome-Wide Identification of Detoxification Genes in Wild Silkworm Antheraea pernyi and Transcriptional Response to Coumaphos

  2023cites this paper
• The genome sequence of the Diamondback Moth, Plutella xylostella (Linnaeus, 1758)

  2023cites this paper
• Midgut serine proteinases participate in dietary adaptations of the castor (Eri) silkworm Samia ricini Anderson transferred from Ricinus communis to an ancestral host, Ailanthus excelsa Roxb

  2023cites this paper
• Novel miR-108 and miR-234 target juvenile hormone esterase to regulate the response of Plutella xylostella to Cry1Ac protoxin.

  2023cites this paper
• Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae

  2023cites this paper
• Identification and characterization of odorant receptors in Plutella xylostella antenna response to 2,3-dimethyl-6-(1-hydroxy)-pyrazine

  2023cites this paper
• Dynamic Roles of Insect Carboxyl/Cholinesterases in Chemical Adaptation

  2023cites this paper
• ABCH2 transporter in the first line of defense protects malaria vectors from pyrethroids

  2023cites this paper
• Oxygen stress on age-stage, two-sex life tables and transcriptomic response of diamondback moth (Plutella xylostella)

  2023cites this paper
• Genome-scale analysis of ABC transporter genes and characterization of the ABCC type transporter genes in the oriental armyworm, Mythimna separata (Walker).

  2023cites this paper
• An LW-Opsin Mutation Changes the Gene Expression of the Phototransduction Pathway: A Cryptochrome1 Mutation Enhances the Phototaxis of Male Plutella xylostella (Lepidoptera: Plutellidae)

  2023cites this paper
• Functional differentiation of two general odorant-binding proteins to sex pheromones in Spodoptera frugiperda.

  2023cites this paper
• Proteome Analysis of Male Accessory Gland Secretions in the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

  2023cites this paper
• Microcosm-omics centric investigation reveals elevated bacterial degradation of imidacloprid.

  2023cites this paper
• Functional characteristics of Dicer genes in Plutella xylostella.

  2023cites this paper
• Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae

  2023cites this paper
• Hsp70 and Hsp90 Elaborately Regulate RNAi Efficiency in Plutella xylostella

  2023cites this paper
• Overexpression of ABCB transporter genes confer multiple insecticide tolerances in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae).

  2023cites this paper
• Methadone maintenance treatment is more effective than compulsory detoxification in addressing gut microbiota dysbiosis caused by heroin abuse

  2023cites this paper
• Glucose Oxidase of a Crucifer-Specialized Insect: A Potential Role in Suppressing Plant Defense via Modulating Antagonistic Plant Hormones.

  2023cites this paper
• Fumigant activity and transcriptomic analysis of two plant essential oils against the tea green leafhopper, Empoasca onukii Matsuda

  2023cites this paper
• Identification and characterization of ABC proteins in an important rice insect pest, Cnaphalocrocis medinalis unveil their response to Cry1C toxin.

  2023cites this paper
• Synthesis and Biological Activity of Sulfamate-Adamantane Derivatives as Glucosinolate Sulfatase Inhibitors.

  2023cites this paper
• Large‐scale genomic data reveal the phylogeny and evolution of owlet moths (Noctuoidea)

  2023cites this paper
• Characterization of methanotrophic community and activity in landfill cover soils under dimethyl sulfide stress.

  2023cites this paper
• PxTret1-like Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution

  2022cites this paper
• Comparative genomic analysis of Echinococcus multilocularis with other tapeworms

  2022cites this paper
• Identification of chorion genes and RNA interference-mediated functional characterization of chorion-1 in Plutella xylostella

  2022cites this paper
• Ontogeny-dependent effects of elevated CO2 and watering frequency on interaction between Aristolochia contorta and its herbivores.

  2022cites this paper
• 2000-2019: twenty years of highly influential publications in molecular plant immunity.

  2022cites this paper
• Functional Diversity of the Lepidopteran ATP-Binding Cassette Transporters

  2022cites this paper
• The genome sequence of the rosy rustic, Hydraecia micacea (Esper, 1789)

  2022cites this paper
• Title: Small silencing RNAs expressed from W-linked retrocopies of Masculinizer target the male-determining gene PxyMasc during female sex determination in the Diamondback moth Plutella xylostella

  2022cites this paper
• Vitelline Membrane Protein 26 Mutagenesis, Using CRISPR/Cas9, Results in Egg Collapse in Plutella xylostella

  2022cites this paper
• Development of CRISPR/Cas9-Mediated Gene-Drive Construct Targeting the Phenotypic Gene in Plutella xylostella

  2022cites this paper
• Genome-wide identification and expression profiling of odorant receptor genes in the malaria vector Anophelessinensis

  2022cites this paper
• A scaffold-level genome assembly of a minute pirate bug, Orius laevigatus (Hemiptera: Anthocoridae), and a comparative analysis of insecticide resistance-related gene families with hemipteran crop pests

  2022cites this paper
• The Genomics and Population Genomics of the Light Brown Apple Moth, Epiphyas postvittana, an Invasive Tortricid Pest of Horticulture

  2022cites this paper
• S0007485321000675jra 187..196

  2022cites this paper
• The oriental armyworm genome yields insights into the long-distance migration of noctuid moths.

  2022cites this paper
• Antibody-Based Methods Reveal the Protein Expression Properties of Glucosinolate Sulfatase 1 and 2 in Plutella xylostella

  2022cites this paper