SCIENCE@home

Quantification of regenerative potential in primary human mammary epithelial cells

J. Linnemann,H. Miura,Lisa K. Meixner,M. Irmler,Uwe J. Kloos,Benjamin Hirschi,H. Bartsch,Steffen Sass,J. Beckers,Fabian J Theis,C. Gabka,K. Sotlar,C. Scheel

Published 2015 in Development

ABSTRACT

We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid or compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is required for alveologenesis. Thereby, branched structures generated in compliant collagen gels resemble terminal ductal-lobular units (TDLUs), the functional units of the mammary gland. Using the membrane metallo-endopeptidase CD10 as a surface marker enriches for TDLU formation and reveals the presence of stromal cells within the CD49fhi/EpCAM− population. In summary, we describe a defined in vitro assay system to quantify cells with regenerative potential and systematically investigate their interaction with the physical environment at distinct steps of morphogenesis. Summary: An assay in which single, freshly isolated human mammary epithelial cells are cultured in a matrix environment is developed and used to quantify the regenerative potential of human mammary cells.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-62 of 62 references · Page 1 of 1

CITED BY

Showing 1-100 of 145 citing papers · Page 1 of 2

12
Next