State of the art in non-animal approaches for skin sensitization testing: from individual test methods towards testing strategies

No abstract is available for this paper.

• Publication year

  2016

• Venue

  Archives of Toxicology

• Publication date

  2016-09-14

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1007/s00204-016-1842-4PMID 27629427
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Genes specifically modulated in sensitized skins allow the detection of sensitizers in a reconstructed human skin model. Development of the SENS-IS assay.

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• Probabilistic hazard assessment for skin sensitization potency by dose–response modeling using feature elimination instead of quantitative structure–activity relationships

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• LLNA variability: An essential ingredient for a comprehensive assessment of non-animal skin sensitization test methods and strategies.

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• Evaluation of an In Vitro Human Dermal Sensitization Test for Use with Medical Device Extracts

  2015influential reference
• Assessment of the sensitizing potency of preservatives with chance of skin contact by the loose-fit coculture-based sensitization assay (LCSA)

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• A fluorescence high throughput screening method for the detection of reactive electrophiles as potential skin sensitizers.

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• Test battery with the human cell line activation test, direct peptide reactivity assay and DEREK based on a 139 chemical data set for predicting skin sensitizing potential and potency of chemicals

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• Evaluation of an optimized protocol using human peripheral blood monocyte derived dendritic cells for the in vitro detection of sensitizers: Results of a ring study in five laboratories.

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• The Myeloid U937 Skin Sensitization Test (U-SENS) addresses the activation of dendritic cell event in the adverse outcome pathway for skin sensitization.

  2015cited by this paper
• EURL ECVAM Recommendation on the human Cell Line Activation Test (h-CLAT) for skin sensitisation testing

  2015cited by this paper
• Bayesian integrated testing strategy (ITS) for skin sensitization potency assessment: a decision support system for quantitative weight of evidence and adaptive testing strategy

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• Predicting skin sensitizer potency based on in vitro data from KeratinoSens and kinetic peptide binding: global versus domain-based assessment.

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• Human T cell priming assay: depletion of peripheral blood lymphocytes in CD25(+) cells improves the in vitro detection of weak allergen-specific T cells.

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• LuSens: a keratinocyte based ARE reporter gene assay for use in integrated testing strategies for skin sensitization hazard identification.

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• Applying the skin sensitisation adverse outcome pathway (AOP) to quantitative risk assessment.

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• Skin sensitization risk assessment model using artificial neural network analysis of data from multiple in vitro assays.

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• Anchoring molecular mechanisms to the adverse outcome pathway for skin sensitization: Analysis of existing data

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• Genomic allergen rapid detection in-house validation--a proof of concept.

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• EURL ECVAM Status Report on the Development, Validation and Regulatory Acceptance of Alternative Methods and Approaches (2013-April 2014)

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• Assessment of the sensitizing potency of preservatives with chance of skin contact by the loose-fit coculture-based sensitization assay (LCSA)

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• Open source software implementation of an integrated testing strategy for skin sensitization potency based on a Bayesian network.

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• Applying Adverse Outcome Pathways (AOPs) to support Integrated Approaches to Testing and Assessment (IATA).

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• Bayesian integrated testing strategy to assess skin sensitization potency: from theory to practice

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• A dataset on 145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation

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• Data integration of non-animal tests for the development of a test battery to predict the skin sensitizing potential and potency of chemicals.

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• Development of a new in vitro skin sensitization assay (Epidermal Sensitization Assay; EpiSensA) using reconstructed human epidermis.

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• Transfer of a two-tiered keratinocyte assay: IL-18 production by NCTC2544 to determine the skin sensitizing capacity and epidermal equivalent assay to determine sensitizer potency.

  2013influential reference
• The GARD assay for assessment of chemical skin sensitizers.

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• From pathways to people: applying the adverse outcome pathway (AOP) for skin sensitization to risk assessment.

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• Predictive performance of the human Cell Line Activation Test (h-CLAT) for lipophilic chemicals with high octanol-water partition coefficients.

  2013cited by this paper
• Integrated testing strategies for safety assessments.

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• NCTC 2544 and IL-18 production: a tool for the identification of contact allergens.

  2013cited by this paper
• Proteomic allergen-peptide/protein interaction assay for the identification of human skin sensitizers.

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• The OSIRIS Weight of Evidence approach: ITS for skin sensitisation.

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• Design and performance of a spreadsheet-based model for estimating bioavailability of chemicals from dermal exposure.

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• Human T cell priming assay (hTCPA) for the identification of contact allergens based on naive T cells and DC--IFN-γ and TNF-α readout.

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• Computer models versus reality: how well do in silico models currently predict the sensitization potential of a substance.

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• Putting the parts together: combining in vitro methods to test for skin sensitizing potentials.

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• Dermal clearance model for epidermal bioavailability calculations.

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• An in vitro method for detecting chemical sensitization using human reconstructed skin models and its applicability to cosmetic, pharmaceutical, and medical device safety testing

  2012influential reference
• Mathematical models of skin permeability: an overview.

  2011cited by this paper
• Mechanisms of chemical‐induced innate immunity in allergic contact dermatitis

  2011cited by this paper
• A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests

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• Determining epidermal disposition kinetics for use in an integrated nonanimal approach to skin sensitization risk assessment.

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• Predictive performance for human skin sensitizing potential of the human cell line activation test (h‐CLAT)

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• A potential in vitro epidermal equivalent assay to determine sensitizer potency.

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• Intralaboratory validation of four in vitro assays for the prediction of the skin sensitizing potential of chemicals.

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• Alternative (non-animal) methods for cosmetics testing: current status and future prospects—2010

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• Keratinocytes, Innate Immunity and Allergic Contact Dermatitis - Opportunities for the Development of In Vitro Assays to Predict the Sensitizing Potential of Chemicals

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• Functionality and specificity of gene markers for skin sensitization in dendritic cells.

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• Chemical allergy: translating biology into hazard characterization.

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• Investigation of peptide reactivity of pro-hapten skin sensitizers using a peroxidase-peroxide oxidation system.

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• Progress on the development of human in vitro dendritic cell based assays for assessment of the sensitizing potential of a compound.

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• Modification of cell-surface thiols elicits activation of human monocytic cell line THP-1: possible involvement in effect of haptens 2,4-dinitrochlorobenzene and nickel sulfate.

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• Dermal sensitization quantitative risk assessment (QRA) for fragrance ingredients.

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• Chemical reactivity indices and mechanism‐based read‐across for non‐animal based assessment of skin sensitisation potential

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• LC-MS-based characterization of the peptide reactivity of chemicals to improve the in vitro prediction of the skin sensitization potential.

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• Identification of mechanisms of toxic action for skin sensitisation using a SMARTS pattern based approach

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• A cell-based in vitro alternative to identify skin sensitizers by gene expression.

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• Allergic contact dermatitis--formation, structural requirements, and reactivity of skin sensitizers.

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• Skin Sensitisation and Epidermal Disposition: The Relevance of Epidermal Disposition for Sensitisation Hazard Identification and Risk Assessment

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• A loose‐fit coculture of activated keratinocytes and dendritic cell‐related cells for prediction of sensitizing potential

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