Cryo-electron tomography provides novel insights into nuclear pore architecture: implications for nucleocytoplasmic transport.

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Journal of Molecular Biology

• Publication date

  2003-04-18

• Fields of study

  Biology, Medicine, Physics

• Identifiers
  DOI 10.1016/S0022-2836(03)00266-3PMID 12684002
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex.

  2002influential reference
• Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm.

  2002cited by this paper
• The vertebrate nuclear pore complex: from structure to function.

  2002cited by this paper
• The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins

  2001cited by this paper
• Gradient of Increasing Affinity of Importin β for Nucleoporins along the Pathway of Nuclear Import

  2001cited by this paper
• Kinetic analysis of translocation through nuclear pore complexes

  2001cited by this paper
• The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism

  2000cited by this paper
• The Yeast Nuclear Pore Complex

  2000cited by this paper
• The nuclear pore complex: from molecular architecture to functional dynamics.

  1999cited by this paper
• Optical recording of signal-mediated protein transport through single nuclear pore complexes.

  1999influential reference
• Nuclear hourglass technique: an approach that detects electrically open nuclear pores in Xenopus laevis oocyte.

  1999cited by this paper
• Determination of the inelastic mean free path of electrons in vitrified ice layers for on‐line thickness measurements by zero‐loss imaging

  1999influential reference
• Transport between the cell nucleus and the cytoplasm.

  1999cited by this paper
• Calcium-mediated structural changes of native nuclear pore complexes monitored by time-lapse atomic force microscopy.

  1999influential reference
• Electron tomography of ice-embedded prokaryotic cells.

  1998cited by this paper
• Major Binding Sites for the Nuclear Import Receptor Are the Internal Nucleoporin Nup153 and the Adjacent Nuclear Filament Protein Tpr

  1998cited by this paper
• Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes

  1998cited by this paper
• ATP-Induced Shape Change of Nuclear Pores Visualized with the Atomic Force Microscope

  1998cited by this paper
• Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications.

  1998cited by this paper
• Active nuclear pore complexes in Chironomus: visualization of transporter configurations related to mRNP export.

  1998cited by this paper
• Perspectives of molecular and cellular electron tomography.

  1997cited by this paper
• Electron Tomography of Single Ice-Embedded Macromolecules: Three-Dimensional Alignment and Classification

  1997cited by this paper
• Visualizing nuclear export of different classes of RNA by electron microscopy.

  1997cited by this paper
• The location of the transport gate in the nuclear pore complex.

  1997influential reference
• Identification of Protein p270/Tpr as a Constitutive Component of the Nuclear Pore Complex–attached Intranuclear Filaments

  1997cited by this paper
• Energy filtered electron tomography of ice-embedded actin and vesicles.

  1997cited by this paper
• Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly.

  1997cited by this paper
• Immunocytochemical evidence for a stepwise assembly of Balbiani ring premessenger ribonucleoprotein particles.

  1997cited by this paper
• AVS software for visualization in molecular microscopy.

  1996cited by this paper
• Molecular dissection of the nuclear pore complex.

  1996influential reference
• Sequential Binding of Import Ligands to Distinct Nucleopore Regions During Their Nuclear Import

  1996cited by this paper
• The EM Program Package: A Platform for Image Processing in Biological Electron Microscopy

  1996cited by this paper
• Structural plasticity of the nuclear pore complex.

  1995influential reference
• Interactions and three-dimensional localization of a group of nuclear pore complex proteins

  1994cited by this paper
• Architecture of the Xenopus nuclear pore complex revealed by three- dimensional cryo-electron microscopy

  1993influential reference
• The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal

  1993cited by this paper
• Isolation of the yeast nuclear pore complex

  1993influential reference
• Intranuclear filaments containing a nuclear pore complex protein

  1993cited by this paper
• Towards automatic electron tomography. II. Implementation of autofocus and low-dose procedures

  1993cited by this paper
• Automated microscopy for electron tomography.

  1992cited by this paper
• High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores

  1992cited by this paper
• Architecture and design of the nuclear pore complex.

  1992cited by this paper
• Towards automatic electron tomography

  1992cited by this paper
• Toward a more complete 3-D structure of the nuclear pore complex.

  1991cited by this paper
• Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components

  1990influential reference
• A major glycoprotein of the nuclear pore complex is a membrane‐spanning polypeptide with a large lumenal domain and a small cytoplasmic tail.

  1990cited by this paper
• Interactions and structure of the nuclear pore complex revealed by cryo- electron microscopy

  1989cited by this paper
• EELS log-ratio technique for specimen-thickness measurement in the TEM.

  1988cited by this paper
• Digital image processing: The semper system

  1979cited by this paper
• Nuclear envelope permeability

  1975cited by this paper
• A new method of preparation of a self-perforated micro plastic grid and its application.

  1965cited by this paper

• Amperometric ion-selective nanoelectrodes for bioanalytical sensing and imaging.

  2026cites this paper
• Karyopherins remodel the dynamic organization of the nuclear pore complex transport barrier

  2025cites this paper
• Nuclear mechanics as a determinant of nuclear pore complex plasticity

  2025cites this paper
• O-GlcNAcylation and an importin-β radial gradient keep the FG barrier liquid in live-cell nuclear pores

  2025cites this paper
• Exploring Cellular Gateways: Unraveling the Secrets of Disordered Proteins within Live Nuclear Pores

  2024cites this paper
• Dynamic molecular mechanism of the nuclear pore complex permeability barrier

  2023cites this paper
• Nuclear Delivery of Nanoparticle-Based Drug Delivery Systems by Nuclear Localization Signals

  2023cites this paper
• Unveiling the complexity: assessing models describing the structure and function of the nuclear pore complex

  2023cites this paper
• Obtaining 3D super-resolution images by utilizing rotationally symmetric structures and 2D-to-3D transformation

  2023cites this paper
• Spelling out the roles of individual nucleoporins in nuclear export of mRNA

  2022cites this paper
• MIT Open Access

  2022cites this paper
• Phase separation of FG-nucleoporins in nuclear pore complexes.

  2022cites this paper
• Free energy calculations shed light on the nuclear pore complex’s selective barrier nature

  2021cites this paper
• Crosstalk between nucleocytoplasmic trafficking and the innate immune response to viral infection

  2021cites this paper
• Self-Targeting of Carbon Dots into the Cell Nucleus: Diverse Mechanisms of Toxicity in NIH/3T3 and L929 Cells

  2021cites this paper
• Challenges and triumphs in cryo-electron tomography

  2021cites this paper
• Physics of the Nuclear Pore Complex: Theory, Modeling and Experiment.

  2021cites this paper
• Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe

  2021cites this paper
• Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.

  2021cites this paper
• Cellular Nanomachines: From Discovery to Structure-Function and Therapeutic Applications

  2020cites this paper
• Traffic jam at the nuclear pore: All roads lead to nucleocytoplasmic transport defects in ALS/FTD.

  2020cites this paper
• Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex

  2020cites this paper
• Nuclear Pore: A Bidirectional Transport Machinery

  2020cites this paper
• The Structure of the Nuclear Pore Complex (An Update).

  2019cites this paper
• Nucleoporin 85 is Crucial for Nuclear Import of HIV-1

  2019cites this paper
• Physiochemical Effects of Nanoparticle on Cell Nuclear Complex Pore Transport: A Coarse-Grained Computational Model.

  2019cites this paper
• NUP214 deficiency causes severe encephalopathy and microcephaly in humans

  2019cites this paper
• 3D Tracking-Free Approach for Obtaining 3D Super-Resolution Information in Rotationally Symmetric Biostructures.

  2019cites this paper
• Recent Advances on the Structure and Function of RNA Acetyltransferase Kre33/NAT10

  2019cites this paper
• Atomic force microscopy reveals structural variability amongst nuclear pore complexes

  2018cites this paper
• Remodelling of the nuclear envelope during KSHV lytic infection

  2018cites this paper
• Nuclear Export of the Thyroid Hormone Receptor [Alpha]

  2018cites this paper
• Moonlighting nuclear pore proteins: tissue-specific nucleoporin function in health and disease

  2018cites this paper
• Nuclear Pore Complexes: Fascinating Nucleocytoplasmic Checkpoints

  2018cites this paper
• Cellular Electron Cryotomography: Toward Structural Biology In Situ.

  2017cites this paper
• Cryo-electron tomography: an ideal method to study membrane-associated proteins

  2017cites this paper
• Investigating molecular crowding within nuclear pores using polarization-PALM

  2017cites this paper
• The advent of structural biology in situ by single particle cryo-electron tomography

  2017cites this paper
• Structural Biology outside the box-inside the cell.

  2017cites this paper
• Kinetics of transport through the nuclear pore complex.

  2017cites this paper
• Structural dynamics of the nuclear pore complex.

  2017cites this paper
• The nuclear pore complex: structure and function

  2017cites this paper
• Modeling of the mechano-chemical behaviour of the nuclear pore complex: current research and perspectives

  2016cites this paper
• The nuclear pore complex: understanding its function through structural insight

  2016cites this paper
• Role of Intermediate Filaments in Vesicular Traffic

  2016cites this paper
• Investigation of the effect of 4'-alkylıklavuzon derivatives on nucleotide synthesis and nucleocytoplasmic transport

  2016cites this paper
• Identification of a Nuclear Localization Signal (NLS) within the Pleckstrin Homology (PH) domain of Rho Guanine Nucleotide Exchange Factor (RGNEF)

  2016influential citation
• Kinetic analysis of karyopherin-mediated transport through the nuclear pore complex

  2016cites this paper
• Nucleocytoplasmic Transport: A Paradigm for Molecular Logistics in Artificial Systems.

  2016cites this paper
• Nucleocytoplasmic trafficking

  2016cites this paper
• Structure Determination of the Nuclear Pore Complex with Three-Dimensional Cryo electron Microscopy

  2016cites this paper
• Impact of the crystallization condition on importin-β conformation.

  2016cites this paper
• Spatiotemporal dynamics of the nuclear pore complex transport barrier resolved by high-speed atomic force microscopy.

  2016cites this paper
• Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.

  2016cites this paper
• A theoretical study of the entry of baculovirus into cell nucleus

  2016cites this paper
• Rapid Brownian Motion Primes Ultrafast Reconstruction of Intrinsically Disordered Phe-Gly Repeats Inside the Nuclear Pore Complex

  2016cites this paper
• Characterizing Importin Binding to Thyroid Hormone Receptor α1

  2016cites this paper
• Translocation de biopolymères à travers des pores naturels ou artificiels

  2016cites this paper
• Cellular structural biology as revealed by cryo-electron tomography

  2016cites this paper
• Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography.

  2016cites this paper
• Nucleoporin 62-Like Protein Activates Canonical Wnt Signaling through Facilitating the Nuclear Import of β-Catenin in Zebrafish

  2015cites this paper
• Complex Commingling: Nucleoporins and the Spindle Assembly Checkpoint

  2015cites this paper
• Nuclear Pore Complexes and Nucleocytoplasmic Transport: From Structure to Function to Disease.

  2015cites this paper
• Developments in cryo-electron tomography for in situ structural analysis.

  2015cites this paper
• Circadian Timing of Curcumin Efficacy and Nuclear Transport Properties of Cancer Cells

  2015cites this paper
• The nuclear pore complex – structure and function at a glance

  2015cites this paper
• Structure and gating of the nuclear pore complex

  2015cites this paper
• Kap-Centric control of nuclear pores based on promiscuous binding to FG nucleoporins

  2014cites this paper
• Exploring nuclear pore complex molecular architecture by immuno-electron microscopy using Xenopus oocytes.

  2014cites this paper
• Functional insights of nucleocytoplasmic transport in plants

  2014cites this paper
• Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy

  2014cites this paper
• Reduction of dimensionality in Karyopherinβ1 mediated transport on FG domains

  2014cites this paper
• The proteasome activator Blm10 facilitates nuclear import of mature proteasome core particles in yeast

  2014cites this paper
• Preparation and mechanism insight of nuclear envelope-like polymer vesicles for facile loading of biomacromolecules and enhanced biocatalytic activity.

  2014cites this paper
• Nucleoporin Gene Fusions and Hematopoietic Malignancies

  2014cites this paper
• Disordered Proteinaceous Machines

  2014cites this paper
• Polymer Theory applied to the Nuclear Pore Complex

  2014cites this paper
• Stress and aging at the nuclear gateway.

  2014cites this paper
• The Structure and Physiology of the Nuclear Pore Complex

  2013cites this paper
• Quantitative Analysis of Membrane Protein Transport Across the Nuclear Pore Complex

  2013cites this paper
• Physical modelling of the nuclear pore complex

  2013cites this paper
• Choreography of importin-α/CAS complex assembly and disassembly at nuclear pores

  2013cites this paper
• Structural analysis of supramolecular assemblies by cryo-electron tomography.

  2013cites this paper
• Dynamics and driving forces of macromolecular complexes.

  2013cites this paper
• Nuclear import of baculovirus autographa californica multiple nucleopolyhedrovirus (AcMNPV)

  2013cites this paper
• Dynamics and diverse functions of nuclear pore complex proteins

  2012cites this paper
• Nuclear transport of baculovirus: revealing the nuclear pore complex passage.

  2012cites this paper
• The transport of integral membrane proteins across the nuclear pore complex

  2012cites this paper
• Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution

  2012cites this paper
• Single-molecule studies of nucleocytoplasmic transport: from one dimension to three dimensions.

  2012cites this paper
• Looking back at a quarter-century of research at the Maurice E. Müller Institute for Structural Biology.

  2012cites this paper
• Multiscale dynamics in nucleocytoplasmic transport.

  2012cites this paper
• The human nuclear pore complex as revealed by cryo-electron tomography.

  2012cites this paper
• Identification and characterization of the Arabidopsis homolog of the yeast TREX-2 complex

  2012cites this paper
• Structure–function mapping of a heptameric module in the nuclear pore complex

  2012cites this paper
• Analytical approaches for studying transporters, channels and porins.

  2012cites this paper
• 2012 Landes Bioscience. Do not distribute. Mapping ubiquitin modifications reveals new functions for the yeast nuclear pore complex

  2012cites this paper
• Limiting factors in single particle cryo electron tomography

  2012cites this paper
• Structural and biochemical analysis of the Y-shaped Nup84 subcomplex of the NPC

  2012influential citation
• Mapping ubiquitin modifications reveals new functions for the yeast nuclear pore complex

  2012cites this paper