Redox-sensitive homodimerization of Pex11p: a proposed mechanism to regulate peroxisomal division

Pex11p (formerly Pmp27) has been implicated in peroxisomal proliferation (Erdmann, R., and G. Blobel. 1995. J. Cell Biol. 128; 509- 523; Marshall, P.A., Y.I. Krimkevich, R.H. Lark, J.M. Dyer, M. Veenhuis, and J.M. Goodman, 1995. J. Cell Biol. 129; 345-355). In its absence, peroxisomes in Saccharomyces cerevisiae fail to proliferate in response to oleic acid; instead, one or two large peroxisomes are formed. Conversely, overproduction of Pex11p causes an increase in peroxisomal number. In this report, we confirm the function of Pex11p in organelle proliferation by demonstrating that this protein can cause fragmentation in vivo of large peroxisomes into smaller organelles. Pex11p is on the inner surface of the peroxisomal membrane. It can form homodimers, and this species is more abundant in mature peroxisomes than in proliferating organelles. Removing one of the three cysteines in the protein inhibits homodimerization. This cysteine 3-->alanine mutation leads to an increase in number and a decrease in peroxisomal density, compared with the wild-type protein, in response to oleic acid. We propose that the active species is the "monomeric" form, and that the increasing oxidative metabolism within maturing peroxisomes causes dimer formation and inhibition of further organelle division.

• Publication year

  1996

• Venue

  Journal of Cell Biology

• Publication date

  1996-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1083/JCB.135.1.123PMID 8858168PMCID 2121026
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Interaction of microtubules with peroxisomes. Tubular and spherical peroxisomes in HepG2 cells and their alterations induced by microtubule-active drugs.

  1996influential reference
• The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop

  1996cited by this paper
• Protein Sorting by Transport Vesicles

  1996cited by this paper
• Coat Proteins and Vesicle Budding

  1996cited by this paper
• Novel Peroxisomal Populations in Subcellular Fractions from Rat Liver

  1995cited by this paper
• The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae

  1995cited by this paper
• Formation of membrane domains created during the budding of vesicular stomatitis virus. A model for selective lipid and protein sorting in biological membranes.

  1995cited by this paper
• An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.

  1995cited by this paper
• Targeted disruption of the alpha isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators

  1995cited by this paper
• Import of Microinjected Proteins Bearing the SKL Peroxisomal Targeting Sequence into the Peroxisomes of a Human Fibroblast Cell Line: Evidence That Virtually All Peroxisomes Are Import‐Competent

  1995cited by this paper
• Import of microinjected proteins bearing the SKL peroxisomal targeting sequence into the peroxisomes of a human fibroblast cell line: evidence that virtually all peroxisomes are import-competent.

  1995cited by this paper
• Pmp27 promotes peroxisomal proliferation

  1995cited by this paper
• Giant peroxisomes in oleic acid-induced Saccharomyces cerevisiae lacking the peroxisomal membrane protein Pmp27p

  1995influential reference
• PPAR: a mediator of peroxisome proliferator action.

  1995cited by this paper
• Budding, fission and domain formation in mixed lipid vesicles induced by lateral phase separation and macromolecular condensation.

  1995cited by this paper
• Localization of peroxisomal 3-oxoacyl-CoA thiolase in particles of varied density in rat liver: implications for peroxisome biogenesis.

  1995cited by this paper
• Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria

  1994cited by this paper
• Regulation of mitochondrial morphology and inheritance by Mdm10p, a protein of the mitochondrial outer membrane

  1994cited by this paper
• The peroxisomal membrane proteins of Candida boidinii: Gene isolation and expression

  1994cited by this paper
• Formation of membrane domains by the envelope proteins of vesicular stomatitis virus.

  1994cited by this paper
• An internal region of the peroxisomal membrane protein PMP47 is essential for sorting to peroxisomes

  1994cited by this paper
• Heterogeneity of peroxisomes in human hepatoblastoma cell line HepG2. Evidence of distinct subpopulations.

  1994cited by this paper
• An oligomeric protein is imported into peroxisomes in vivo

  1994cited by this paper
• MMM1 encodes a mitochondrial outer membrane protein essential for establishing and maintaining the structure of yeast mitochondria

  1994cited by this paper
• Budding and fission of vesicles.

  1993cited by this paper
• Domain-induced budding of fluid membranes.

  1993cited by this paper
• Biogenesis of peroxisomes: isolation and characterization of two distinct peroxisomal populations from normal and regenerating rat liver

  1993cited by this paper
• Specific cross-linking of the proline isomerase cyclophilin to a non-proline-containing peptide.

  1993cited by this paper
• Viral liposomes released from insect cells infected with recombinant baculovirus expressing the matrix protein of vesicular stomatitis virus

  1993cited by this paper
• RTG1 and RTG2: two yeast genes required for a novel path of communication from mitochondria to the nucleus.

  1993cited by this paper
• Shape changes of giant liposomes induced by an asymmetric transmembrane distribution of phospholipids.

  1992cited by this paper
• Shape transitions and shape stability of giant phospholipid vesicles in pure water induced by area-to-volume changes.

  1991cited by this paper
• Peroxisomes induced in Candida boidinii by methanol, oleic acid and D-alanine vary in metabolic function but share common integral membrane proteins.

  1990cited by this paper
• Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae

  1990cited by this paper
• Peroxisomal assembly: membrane proliferation precedes the induction of the abundant matrix proteins in the methylotrophic yeast Candida boidinii.

  1990cited by this paper
• Biochemical mechanisms of induction of hepatic peroxisome proliferation.

  1989cited by this paper
• A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

  1989cited by this paper
• A new peroxisomal disorder with enlarged peroxisomes and a specific deficiency of acyl-CoA oxidase (pseudo-neonatal adrenoleukodystrophy).

  1988cited by this paper
• Metabolic Significance and Biogenesis of Microbodies in Yeasts

  1987cited by this paper
• Rapid and efficient site-specific mutagenesis without phenotypic selection.

  1987cited by this paper
• Partial deletion of membrane-bound domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase eliminates sterol-enhanced degradation and prevents formation of crystalloid endoplasmic reticulum

  1987cited by this paper
• Rhabdovirus Membrane and Maturation

  1987cited by this paper
• Three-dimensional reconstruction of a peroxisomal reticulum in regenerating rat liver: evidence of interconnections between heterogeneous segments

  1987cited by this paper
• The membrane proteins of the methanol-induced peroxisome of Candida boidinii. Initial characterization and generation of monoclonal antibodies.

  1986cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Development of crystalline peroxisomes in methanol-grown cells of the yeast Hansenula polymorpha and its relation to environmental conditions

  1978cited by this paper
• Cytochrome P-450 induction by phenobarbital and 3-methylcholanthrene in primary cultures of hepatocytes.

  1976cited by this paper
• Growth of Hansenula polymorpha in a methanol-limited chemostat

  1976cited by this paper
• Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions.

  1974cited by this paper
• A rapid, sensitive, and specific method for the determination of protein in dilute solution.

  1973cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970influential reference

• Mitochondrial retrograde control of transcription evolves with respiratory stress, metabolic adaptation, and virulence in budding yeasts

  2026cites this paper
• Molecular basis of the glycosomal targeting of PEX11 and its mislocalization to mitochondrion in trypanosomes

  2023cites this paper
• Methods to Analyze the Redox Reactivity of Plant Proteins.

  2022cites this paper
• How to improve the production of peptidyl compounds in filamentous fungi

  2022cites this paper
• The Many Faces of Amphipathic Helices

  2018cites this paper
• Peroxisomes: role in cellular ageing and age related disorders

  2018cites this paper
• Redox Regulation of Homeostasis and Proteostasis in Peroxisomes.

  2018cites this paper
• Pro- and Antioxidant Functions of the Peroxisome-Mitochondria Connection and Its Impact on Aging and Disease

  2017cites this paper
• Functional regions of the peroxin Pex19 necessary for peroxisome biogenesis

  2017cites this paper
• Peroxisomal Pex11 is a pore-forming protein homologous to TRPM channels.

  2016cites this paper
• Oxidative Stress and its Role in Peroxisome Homeostasis in Plants

  2016cites this paper
• Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins*

  2016cites this paper
• Role of Pex11p in Lipid Homeostasis in Yarrowia lipolytica

  2015cites this paper
• Giant peroxisomes in a moss (Physcomitrella patens) peroxisomal biogenesis factor 11 mutant

  2015cites this paper
• Genome-Wide Localization Study of Yeast Pex11 Identifies Peroxisome–Mitochondria Interactions through the ERMES Complex

  2015cites this paper
• Oxidative stress, mitochondrial and proteostasis malfunction in adrenoleukodystrophy: A paradigm for axonal degeneration.

  2015cites this paper
• The Functions of Pex11 Family Proteins in Peroxisome Biology

  2014cites this paper
• Redox regulated peroxisome homeostasis

  2014cites this paper
• Expanding the Clinical Phenotypes of Peroxisome Biogenesis Disorders: PEX11 Function in Health and Disease

  2014cites this paper
• Peroxisome biogenesis and dynamics in Hansenula polymorpha

  2014cites this paper
• Peroxisomes take shape

  2013cites this paper
• Redox-regulated Cargo Binding and Release by the Peroxisomal Targeting Signal Receptor, Pex5*

  2013cites this paper
• Self-Interaction of Human Pex11pβ during Peroxisomal Growth and Division

  2013cites this paper
• The peroxisome: an update on mysteries

  2012cites this paper
• Molecular mechanisms of peroxisome biogenesis in yeasts

  2012cites this paper
• The peroxisome: an update on mysteries 2.0

  2012cites this paper
• Phosphorylation-dependent Pex11p and Fis1p interaction regulates peroxisome division

  2012cites this paper
• Docosahexaenoic acid mediates peroxisomal elongation, a prerequisite for peroxisome division

  2012cites this paper
• Fission and proliferation of peroxisomes.

  2012influential citation
• Involvement of glutathione peroxidase 1 in growth and peroxisome formation in Saccharomyces cerevisiae in oleic acid medium.

  2012cites this paper
• The Peroxin Pex34p Functions with the Pex11 Family of Proteins to Regulate the Peroxisome Population in the Yeast Saccharomyces cervisiae

  2012cites this paper
• PEX11 proteins attract Mff and human Fis1 to coordinate peroxisomal fission

  2012cites this paper
• Biogenesis of peroxisomes in mammalian cells: Characterization of the Pex11 proteins and their role in peroxisomal growth and division

  2011cites this paper
• Glycolysis in the African Trypanosome: Targeting Enzymes and Their Subcellular Compartments for Therapeutic Development

  2011cites this paper
• Peroxisomes: membrane events accompanying peroxisome proliferation.

  2011cites this paper
• A Subtle Interplay Between Three Pex11 Proteins Shapes De Novo Formation and Fission of Peroxisomes

  2011cites this paper
• A defect of peroxisomal membrane protein 38 causes enlargement of peroxisomes.

  2011cites this paper
• Target Identification and Intervention Strategies against Kinetoplastid Protozoan Parasites

  2011cites this paper
• Membrane elongation factors in organelle maintenance: the case of peroxisome proliferation

  2011influential citation
• Pex11pβ-mediated growth and division of mammalian peroxisomes follows a maturation pathway

  2010cites this paper
• Membrane curvature during peroxisome fission requires Pex11

  2010cites this paper
• The Arabidopsis Chloroplast Division Protein DYNAMIN-RELATED PROTEIN5B Also Mediates Peroxisome Division[W]

  2010cites this paper
• Inheritance of peroxisomes in the yeast Yarrowia lipolytica

  2010cites this paper
• PEX11 family members are membrane elongation factors that coordinate peroxisome proliferation and maintenance

  2010influential citation
• Molecular basis of peroxisome division and proliferation in plants.

  2010cites this paper
• Salt Stress Causes Peroxisome Proliferation, but Inducing Peroxisome Proliferation Does Not Improve NaCl Tolerance in Arabidopsis thaliana

  2010cites this paper
• Peroxisome biogenesis: something old, something new, something borrowed.

  2010cites this paper
• Hansenula polymorpha pex11 cells are affected in peroxisome retention

  2009cites this paper
• Dynamics of peroxisome abundance: a tale of division and proliferation.

  2009cites this paper
• Comprehensive sequence and expression profile analysis of Hsp20 gene family in rice

  2009cites this paper
• Phosphorylation-dependent Activation of Peroxisome Proliferator Protein PEX11 Controls Peroxisome Abundance*

  2009cites this paper
• Spatiotemporal dynamics of the ER-derived peroxisomal endomembrane system.

  2009cites this paper
• Regulation of peroxisome dynamics.

  2009cites this paper
• ER stress response, peroxisome proliferation, mitochondrial unfolded protein response and Golgi stress response

  2009cites this paper
• UvA-DARE ( Digital Academic Repository ) Metabolite transport across the peroxisomal membrane

  2008cites this paper
• Comprehensive sequence and expression profile analysis of PEX11 gene family in rice.

  2008cites this paper
• Génétique et physiologie cellulaire

  2008cites this paper
• Arabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of Peroxisomes[W]

  2008cites this paper
• The role of peroxisomal metabolism in virulence of Candida albicans

  2007cites this paper
• Dysferlin domain-containing proteins, Pex30p and Pex31p, localized to two compartments, control the number and size of oleate-induced peroxisomes in Pichia pastoris.

  2007cites this paper
• Metabolite transport across the peroxisomal membrane.

  2007cites this paper
• The PEROXIN11 Protein Family Controls Peroxisome Proliferation in Arabidopsis[W]

  2007cites this paper
• Peroxins: A Proliferation Romance amongst Supposition and Disposition

  2007cites this paper
• Environmental response of yeast peroxisomes

  2007cites this paper
• Maintaining peroxisome populations: a story of division and inheritance.

  2007cites this paper
• Aberrant peroxisome morphology in peroxisomal beta-oxidation enzyme deficiencies.

  2006cites this paper
• An intimate collaboration between peroxisomes and lipid bodies

  2006cites this paper
• Growth and division of peroxisomes.

  2006cites this paper
• Overproduction of a single protein, Pc-Pex11p, results in 2-fold enhanced penicillin production by Penicillium chrysogenum.

  2005cites this paper
• The control of peroxisome number and size during division and proliferation.

  2005cites this paper
• Structural, functional and genetic aspects of peroxisome biogenesis

  2005cites this paper
• Dynamin‐related proteins and Pex11 proteins in peroxisome division and proliferation

  2005cites this paper
• Biogenesis of peroxisomes and glycosomes: trypanosomatid glycosome assembly is a promising new drug target.

  2004cites this paper
• Heterologe Expression des AAA-Proteins Pex6p in der Hefe Saccharomyces cerevisiae

  2004cites this paper
• Visualization and quantitation of peroxisomes using fluorescent nanocrystals: treatment of rats and monkeys with fibrates and detection in the liver.

  2004cites this paper
• An Arabidopsis dynamin-related protein, DRP3A, controls both peroxisomal and mitochondrial division.

  2004cites this paper
• The role of Pex3p in early events of peroxisome biogenesis in Hansenula polymorpha

  2003cites this paper
• Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome

  2003cites this paper
• YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae

  2003cites this paper
• The role of Pex3p in early events of peroxisome biogenesis in Hansenula polymorpha

  2003cites this paper
• Peroxisome biogenesis.

  2003cites this paper
• Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae.

  2003influential citation
• Peroxisome assembly in yeast

  2003cites this paper
• Transcriptional Down-regulation of Peroxisome Numbers Affects Selective Peroxisome Degradation in Hansenula polymorpha*

  2003cites this paper
• Reviews of Physiology, Biochemistry and Pharmacology

  2003cites this paper
• Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis.

  2003cites this paper
• Transcriptome profiling to identify genes involved in peroxisome assembly and function

  2002influential citation
• Jcb: Article Introduction

  2002cites this paper
• PEX11 promotes peroxisome division independently of peroxisome metabolism

  2002influential citation
• Import of Proteins into Peroxisomes

  2002cites this paper
• Plant Peroxisomes

  2002cites this paper
• Peroxisomal Biogenesis and Acquistion of Membrane Proteins

  2002cites this paper
• Peroxisome biogenesis.

  2001cites this paper
• Identification of a Peroxisomal ATP Carrier Required for Medium-Chain Fatty Acid β-Oxidation and Normal Peroxisome Proliferation in Saccharomyces cerevisiae

  2001cites this paper
• The life cycle of the peroxisome

  2001cites this paper
• A Novel Method to Determine the Topology of Peroxisomal Membrane Proteins in Vivo Using the Tobacco Etch Virus Protease*

  2001cites this paper
• Sorting of matrix proteins to peroxisomes in the methylotrophic yeast Hansenula polymorpha

  2001cites this paper
• Genome-wide responses to mitochondrial dysfunction.

  2001cites this paper
• Genome-wide Responses to Mitochondrial Dysfunction h V

  2001influential citation
• Pex11p Plays a Primary Role in Medium-Chain Fatty Acid Oxidation, a Process That Affects Peroxisome Number and Size in Saccharomyces cerevisiae

  2000influential citation