Analysis of the local organization and dynamics of cellular actin networks

Live cell imaging, high-resolution microscopy, and computational modeling show that dynamic formin-filamin-actin asters self-organize into an actomyosin contractile network that may maintain mechanical coherence of cytoplasm.

• Publication year

  2013

• Venue

  Journal of Cell Biology

• Publication date

  2013-09-30

• Fields of study

  Biology, Medicine, Physics

• Identifiers
  DOI 10.1083/jcb.201210123PMID 24081490PMCID 3787384
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Hierarchical self-organization of cytoskeletal active networks

  2012cited by this paper
• Triggering a Cell Shape Change by Exploiting Preexisting Actomyosin Contractions

  2012influential reference
• Continuum Percolation Thresholds in Two Dimensions

  2012cited by this paper
• α-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly

  2012cited by this paper
• General mechanism of actomyosin contractility.

  2011cited by this paper
• Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

  2011influential reference
• Punctuated actin contractions during convergent extension and their permissive regulation by the non-canonical Wnt-signaling pathway

  2011cited by this paper
• Active multistage coarsening of actin networks driven by myosin motors

  2011cited by this paper
• Reconstitution of contractile actomyosin bundles.

  2011cited by this paper
• Assembly and architecture of precursor nodes during fission yeast cytokinesis

  2011influential reference
• Micropatterning on glass with deep UV

  2011cited by this paper
• Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells.

  2010cited by this paper
• Planar polarized actomyosin contractile flows control epithelial junction remodelling

  2010cited by this paper
• Nanoscale architecture of integrin-based cell adhesions

  2010cited by this paper
• Unleashing formins to remodel the actin and microtubule cytoskeletons

  2010cited by this paper
• Kinetics of myosin node aggregation into a contractile ring.

  2010cited by this paper
• Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions

  2010cited by this paper
• DAAM1 Is a Formin Required for Centrosome Re-Orientation during Cell Migration

  2010influential reference
• Single particle tracking

  2010cited by this paper
• Cytoskeletal coherence requires myosin-IIA contractility

  2010cited by this paper
• Mechanics of cytokinesis in eukaryotes.

  2010cited by this paper
• QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

  2010cited by this paper
• Force generated by actomyosin contraction builds bridges between adhesive contacts

  2010cited by this paper
• Identification and characterization of a small molecule inhibitor of formin-mediated actin assembly.

  2009influential reference
• Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure

  2009cited by this paper
• SINGLE-PARTICLE TRACKING

  2009cited by this paper
• Pulsed contractions of an actin–myosin network drive apical constriction

  2009cited by this paper
• Liquids and Structural Glasses Special Feature: An active biopolymer network controlled by molecular motors

  2009cited by this paper
• Force propagation across cells: mechanical coherence of dynamic cytoskeletons.

  2009cited by this paper
• The shape of motile cells.

  2009cited by this paper
• Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast

  2008cited by this paper
• Lifeact: a versatile marker to visualize F-actin

  2008cited by this paper
• G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers

  2008cited by this paper
• Nanoclusters of GPI-anchored proteins are formed by cortical actin-driven activity.

  2008cited by this paper
• Collective Langevin dynamics of flexible cytoskeletal fibers

  2007cited by this paper
• Nonequilibrium Mechanics of Active Cytoskeletal Networks

  2007cited by this paper
• Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis.

  2007influential reference
• Lamellipodial actin mechanically links myosin activity with adhesion-site formation.

  2007cited by this paper
• Imaging Intracellular Fluorescent Proteins at Nanometer Resolution

  2006cited by this paper
• Development and application of automatic high-resolution light microscopy for cell-based screens.

  2006cited by this paper
• Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast

  2006influential reference
• Nonlinear competition between asters and stripes in filament-motor systems

  2005cited by this paper
• Oligomerization of the diaphanous‐related formin FHOD1 requires a coiled‐coil motif critical for its cytoskeletal and transcriptional activities

  2005cited by this paper
• Cortical flows powered by asymmetrical contraction transport PAR proteins to establish and maintain anterior-posterior polarity in the early C. elegans embryo.

  2004influential reference
• Homo-oligomerization Is Essential for F-actin Assembly by the Formin Family FH2 Domain*

  2004cited by this paper
• A Syntaxin 1, Gαo, and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization

  2004cited by this paper
• Periodic lamellipodial contractions correlate with rearward actin waves.

  2004cited by this paper
• Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography

  2002cited by this paper
• Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds

  2001cited by this paper
• Efficient measurement of the percolation threshold for fully penetrable discs

  2000cited by this paper
• Polarity sorting of actin filaments in cytochalasin-treated fibroblasts.

  1997cited by this paper
• Analysis of the Actin–Myosin II System in Fish Epidermal Keratocytes: Mechanism of Cell Body Translocation

  1997cited by this paper
• Single particle tracking. Analysis of diffusion and flow in two-dimensional systems.

  1991cited by this paper
• Cytoskeleton of mouse embryo fibroblasts. Electron microscopy of platinum replicas.

  1984cited by this paper
• Action of cytochalasin D on cytoskeletal networks

  1982cited by this paper

• Circumferential actomyosin bundles anchored by CCM1 drive endothelial cell contraction and vessel constriction

  2025cites this paper
• Traction-regulated persistence governs durotaxis across cell types.

  2025cites this paper
• A multicellular star-shaped actin network underpins epithelial organization and connectivity

  2025influential citation
• Astral architecture can enhance mechanical strength of cytoskeletal networks by modulating percolation thresholds

  2025influential citation
• Spatiotemporal control of structure and dynamics in a polar active fluid.

  2024cites this paper
• Rigidity percolation and active advection synergize in the actomyosin cortex to drive amoeboid cell motility.

  2024cites this paper
• Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis

  2024cites this paper
• Segregation, Finite Time Elastic Singularities and Coarsening in Renewable Active Matter

  2024cites this paper
• Actomyosin clusters as active units shaping living matter.

  2024cites this paper
• Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.

  2023cites this paper
• Evolution of the Concepts of Architecture and Supramolecular Dynamics of the Plasma Membrane

  2023cites this paper
• Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary†

  2023cites this paper
• Direct investigation of cell contraction signal networks by light-based perturbation methods

  2023cites this paper
• Characterization of Breast Cancer Aggressiveness by Cell Mechanics

  2023cites this paper
• Advected percolation in the actomyosin cortex drives amoeboid cell motility

  2022cites this paper
• Lifetime of actin-dependent protein nanoclusters

  2022cites this paper
• Cancer cells can be killed mechanically or with combinations of cytoskeletal inhibitors

  2022influential citation
• Versatile patterns in the actin cortex of motile cells: Self-organized pulses can coexist with macropinocytic ring-shaped waves

  2022cites this paper
• Crosstalk between myosin II and formin functions in the regulation of force generation and actomyosin dynamics in stress fibers

  2021cites this paper
• The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces

  2021cites this paper
• The role of the Arp2/3 complex in shaping the dynamics and structures of branched actomyosin networks

  2020cites this paper
• Stratification relieves constraints from steric hindrance in the generation of compact actomyosin asters at the membrane cortex

  2020cites this paper
• Stress fibers are embedded in a contractile cortical network

  2020cites this paper
• Stress fibers are embedded in a contractile cortical network

  2020cites this paper
• Rapid assembly of a polar network architecture drives efficient actomyosin contractility

  2020cites this paper
• Extracellular | Tension and Tissue Morphogenesis

  2020cites this paper
• Elasticity spectra as a tool to investigate actin cortex mechanics

  2020cites this paper
• Cofilin is required for polarization of tension in stress fiber networks during migration

  2020cites this paper
• Dynamic heterogeneity and non-Gaussian statistics for ganglioside GM1s and acetylcholine receptors on live cell membrane

  2020cites this paper
• Terminal chemical functions of polyamidoamine dendrimer surfaces and its impact on bone cell growth.

  2019cites this paper
• Cytoskeletal actin patterns shape mast cell activation

  2019cites this paper
• Large and reversible myosin-dependent forces in rigidity sensing

  2019cites this paper
• A Tale of Two States: Normal and Transformed, With and Without Rigidity Sensing.

  2019cites this paper
• Nanoscale Architecture of the Cortical Actin Cytoskeleton in Embryonic Stem Cells.

  2019cites this paper
• Emergent spatiotemporal dynamics of the actomyosin network in the presence of chemical gradients.

  2019cites this paper
• Spatio-Temporal Regulation of RhoGTPases Signaling by Myosin II

  2019cites this paper
• Large and reversible myosin-dependent forces in rigidity sensing

  2019cites this paper
• Efficient simulation of thermally fluctuating biopolymers immersed in fluids on 1-micron, 1-second scales

  2019cites this paper
• Modeling membranes under stress: dynamics and disease

  2018cites this paper
• Compartmentalization of the plasma membrane.

  2018cites this paper
• Active bundles of polar and bipolar filaments.

  2018cites this paper
• Emergent mechanics of actomyosin drive punctuated contractions and shape network morphology in the cell cortex

  2018cites this paper
• Morphogenetic degeneracies in the actomyosin cortex

  2018cites this paper
• Polarity sorting drives remodeling of actin-myosin networks

  2018cites this paper
• Morphogenetic degeneracies in the 1 actomyosin cortex

  2018cites this paper
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  2018cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
• Existence and uniqueness for a coupled PDE model for motor-induced microtubule organization

  2017cites this paper
• Effects of cytoskeletal drugs on actin cortex elasticity

  2017cites this paper
• Cortical actin nodes: Their dynamics and recruitment of podosomal proteins as revealed by super-resolution and single-molecule microscopy

  2017cites this paper
• The quantum basis of spatiotemporality in perception and consciousness.

  2017cites this paper
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  2016cites this paper
• Focusing super resolution on the cytoskeleton

  2016cites this paper
• Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

  2016cites this paper
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  2016cites this paper
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  2016cites this paper
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  2016cites this paper
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  2016cites this paper
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  2016cites this paper
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  2016cites this paper
• Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer

  2016cites this paper
• Cortical actin and the plasma membrane: inextricably intertwined.

  2016cites this paper
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• Control of lipid domain organization by a biomimetic contractile actomyosin cortex

  2016cites this paper
• Open Peer Review

  2016cites this paper
• Dynamic heterogeneity and non-Gaussian statistics for acetylcholine receptors on live cell membrane

  2016cites this paper
• Specification of Architecture and Function of Actin Structures by Actin Nucleation Factors.

  2015cites this paper
• Rheology of microgels in single particle confinement

  2015cites this paper
• Formation of contractile networks and fibers in the medial cell cortex through myosin‐II turnover, contraction, and stress‐stabilization

  2015influential citation
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  2015cites this paper
• F-actin distribution and function during sexual development in Eimeria maxima

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• Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim

  2015cites this paper
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  2015cites this paper
• Computational model of polarized actin cables and cytokinetic actin ring formation in budding yeast

  2015cites this paper
• SMIFH2 has effects on Formins and p53 that perturb the cell cytoskeleton

  2015cites this paper
• The connexin 43/ZO-1 complex regulates cerebral endothelial F-actin architecture and migration.

  2015cites this paper
• Tropomyosin Controls Sarcomere-like Contractions for Rigidity Sensing and Suppressing Growth on Soft Matrices

  2015cites this paper
• Supported Lipid Bilayer Technology for the Study of Cellular Interfaces

  2015cites this paper
• Describing Directional Cell Migration with a Characteristic Directionality Time

  2015cites this paper
• Mechanoresponsive, omni-directional and local matrix-degrading actin protrusions in human mesenchymal stem cells microencapsulated in a 3D collagen matrix.

  2015cites this paper
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  2015cites this paper
• Active tension: the role of cadherin adhesion and signaling in generating junctional contractility.

  2015cites this paper
• Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells

  2014cites this paper
• Cortical actomyosin network organization in epithelial cells

  2014cites this paper
• The interaction between Shroom3 and Rho-kinase is required for neural tube morphogenesis in mice

  2014cites this paper
• Polarised cell migration: intrinsic and extrinsic drivers.

  2014cites this paper
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  2014cites this paper
• Report Cellular Control of Cortical Actin Nucleation

  2014cites this paper
• Apical constriction: themes and variations on a cellular mechanism driving morphogenesis

  2014cites this paper
• Investigating the molecular mechanism of Shroom-Rock interaction and its role in cellular and tissue morphogenesis

  2014cites this paper
• Documentation and Localization of Force-mediated Filamin A Domain Perturbations in Moving Cells

  2014cites this paper
• Formins as effector proteins of Rho GTPases

  2014cites this paper
• Contractility Driven Self-Organization of the Actin Cytoskeleton

  2014cites this paper
• Pulsatile contractility of actomyosin networks organizes the cellular cortex at lateral cadherin junctions.

  2014cites this paper