Suppressive effects of cyclosporin A on the induction of alloreactivity in vitro and in vivo.

The purpose of the present study was the investigation of the effect of cyclosporin A (CsA) on the induction of alloreactivity in vitro and in vivo. Addition of CsA to mouse mixed lymphocyte cultures (MLC) not only inhibited lymphocyte proliferation but also prevented the generation of alloreactive cytolytic lymphocytes (CL). It was necessary to add CsA within the first 3 days of a 5-day MLC in order to achieve a significant suppressive effect. Lymphocytes, after being cultured in MLC with CsA for 4 days or longer, were incapable of being activated upon re-exposure to the same alloantigens although their responses to unrelated antigens remained intact, indicating antigen specificity of the suppression induced by CsA and its long-lasting effect. Furthermore, lymphocytes from mice treated with CsA after allosensitization failed to manifest primary cytotoxicity and could not be reactivated in a secondary MLC. Finally, CsA had no effect on those CL already generated, suggesting that CsA acts upon the induction of CL rather than the effector phase.

• Publication year

  1981

• Venue

  Journal of Immunology

• Publication date

  1981-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.127.1.89PMID 6453900
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Biological effects of cyclosporin A: A new antilymphocytic agent

  2005cited by this paper
• IMMUNE RESPONSES TO ORGAN ALLOGRAFTS: III. MARKED DECREASE IN MEDULLARY THYMOCYTES AND SPLENIC T LYMPHOCYTES AFTER CYCLOSPORIN A TREATMENT

  1981cited by this paper
• STUDIES ON THE IMMUNOSUPPRESSIVE PROPERTIES OF CYCLOSPORIN A IN RATS RECEIVING RENAL ALLOGRAFTS

  1980cited by this paper
• Effect of cyclosporin A on human lymphocyte responses in vitro. I. CsA allows for the expression of alloantigen-activated suppressor cells while preferentially inhibiting the induction of cytolytic effector lymphocytes in MLR.

  1980cited by this paper
• Effects of cyclosporin A on the generation of primed lymphocytes in vitro.

  1980cited by this paper
• Rejection of long-surviving mouse skin allografts after withdrawal of cyclosporin A therapy.

  1980cited by this paper
• Cyclosporin A, a powerful immunosuppressant in vivo and in vitro in the dog, fails to induce tolerance.

  1980cited by this paper
• Cyclosporin A and dexamethasone suppress T cell responses by selectively acting at distinct sites of the triggering process.

  1980cited by this paper
• Prolongation of kidney survival during and after cyclosporin A therapy.

  1979cited by this paper
• Cyclosporin A: in vivo and in vitro suppression of rat T-lymphocyte function.

  1979cited by this paper
• Use of cyclosporin A in allogeneic bone marrow transplantation in the rat

  1979cited by this paper
• Selective effects of cyclosporin A on colony-forming lymphoid and myeloid cells in man

  1979cited by this paper
• Nature of the unresponsiveness induced by cyclosporin A in rats bearing renal allografts.

  1979cited by this paper
• CYCLOSPORIN A: AN IMMUNOSUPPRESSIVE AGENT PREFERENTIALLY ACTIVE AGAINST PROLIFERATING T CELLS

  1979cited by this paper
• Induction of specific tolerance in rabbits by kidney allografting and short periods of cyclosporin-A treatment.

  1979cited by this paper
• Cyclosporin A in patients receiving renal allografts from cadaver donors.

  1978cited by this paper
• Cyclosporin A for the treatment of graft-versus-host disease in man.

  1978cited by this paper
• Effects of the new anti-lymphocytic peptide cyclosporin A in animals.

  1977cited by this paper

• Cyclosporin A–Immunobiological Aspects

  2007cites this paper
• Cellular Immune Responses to Allografts and Cytomegalovirus

  2003cites this paper
• Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1269 Cellular Immune Responses to Allografts and Cytomegalovirus

  2003cites this paper
• Oxidative organ damage in a rat model of thermal injury: the effect of cyclosporin A.

  1997cites this paper
• Induction of alloantigen‐specific hyporesponsiveness in vitro by n‐butyrate: antagonistic effect of cyclosporin A

  1996cites this paper
• A protocol with FK 506 for inducing unresponsiveness to murine islet allografts.

  1995cites this paper
• Alteration of spleen lymphocyte populations in rats with arthritis induced by muramyl dipeptide analogue or complete adjuvant.

  1995cites this paper
• Immune modulating therapies for idiopathic inflammatory bowel diseases.

  1994cites this paper
• Lysophospholipid-mediated inhibition of Na+,K(+)-adenosine triphosphatase is a possible mechanism of immunosuppressive activity of cyclosporin A.

  1993cites this paper
• Cyclosporin. A review of its pharmacodynamic and pharmacokinetic properties, and therapeutic use in immunoregulatory disorders.

  1993cites this paper
• Varicella in pediatric renal transplant recipients.

  1992cites this paper
• The in vitro immunosuppressive effect of deoxymethylspergualin in man as compared with FK506 and cyclosporine.

  1992cites this paper
• Brief treatment with rapamycin in vivo increases responsiveness to alloantigens measured by the mixed lymphocyte response.

  1992cites this paper
• Synergistic and antagonistic effects of combinations of cyclosporine A and its metabolites on inhibition of phytohemagglutinin-induced lymphocyte transformation in vitro.

  1991cites this paper
• Generation of a long-lived IgE response in high and low responder strains of rat by co-administration of ricin and antigen.

  1991cites this paper
• Glucocorticoid resistance in chronic asthma. Peripheral blood T lymphocyte activation and comparison of the T lymphocyte inhibitory effects of glucocorticoids and cyclosporin A.

  1991cites this paper
• Cyclosporin A renders target cells resistant to immune cytolysis

  1991cites this paper
• Effect of listeria infection on the allotransplantation reaction in cyclosporin A treated mice.

  1990cites this paper
• In vitro immunosuppressive effect: a comparative study between deoxymethylspergualin and cyclosporin A, on human lymphocyte responses.

  1990cites this paper
• In vivo effects of cyclosporin A on murine B-cells responding to type III pneumococcal polysaccharide.

  1990cites this paper
• Effect of the immune modulating agents cyclophosphamide, methotrexate, hydrocortisone, and cyclosporin A on an animal model of granulomatous bowel disease.

  1990cites this paper
• Immunosuppressive drugs in inflammatory bowel disease. A review of their mechanisms of efficacy and place in therapy.

  1989cites this paper
• The T cell as a therapeutic target

  1989cites this paper
• Immunosuppressive Drugs in Inflammatory Bowel Disease

  1989cites this paper
• The suppressor factor NBxFO is not species-specific.

  1989cites this paper
• Inhibitory effects of cyclosporin A on lymphocyte activation

  1989cites this paper
• Infection and cyclosporine.

  1989cites this paper
• Combination therapy of cyclosporine with steroid inhibits γ-interferon and interleukin-1 gene expression at the level of mRNA synthesisin vivo

  1989cites this paper
• Alpha-difluoromethylornithine, an inhibitor of polyamine biosynthesis, augments cyclosporin A inhibition of cytolytic T lymphocyte induction.

  1989cites this paper
• The effects of immunosuppressive pharmacological agents on the induction of cytotoxic and suppressor T lymphocytes in vitro.

  1988cites this paper
• Regulation of lymphocyte growth by antagonists of interleukin-2 or its cellular receptor

  1988cites this paper
• Strategies for Specific Immunosuppression in Vitro with Cyclosporine

  1988cites this paper
• Effect of cyclosporine A on local inflammation in rejecting allografts.

  1988cites this paper
• EFFECTS OF IMMUNOSUPPRESSIVE DRUGS ON FUNCTIONS OF ACTIVATED T LYMPHOCYTES: CYCLOSPORINE INHIBITION OF GAMMA INTERFERON PRODUCTION IN THE PRESENCE OF INTERLEUKIN 21

  1988cites this paper
• THE IN VIVO IMMUNOSUPPRESSIVE ACTION OF SUPPRESSOR CELLS FROM ALLOANTIGEN‐CYCLOSPORINE-TREATED MICE AND THE CAPACITY OF SPLEEN CELLS TO RELEASE INTERLEUKINS AND -INTERFERON1

  1988cites this paper
• Influence of Cyclosporine on CTL Behavior in Vitro and in Vivo a

  1988cites this paper
• The effects of immunosuppressive pharmacological agents on the induction of cytotoxic and suppressor T lymphocytes in vitro.

  1988cites this paper
• In vivo mechanisms of alloreactivity--IV. Cyclosporine differentially impairs accumulation of donor-reactive CTL but not donor-reactive alloantibody in murine sponge matrix allografts.

  1988cites this paper
• Trough levels and concentration time curves of cyclosporine in patients undergoing renal transplantation

  1988cites this paper
• INVERSE CORRELATION OF CYCLOSPORINE BINDING WITH SENSITIVITY AND RESISTANCE1

  1988cites this paper
• Cyclosporin-resistant and -sensitive T-lymphocyte subsets.

  1987cites this paper
• Suppression of thymidine incorporation into alloresponding adult peripheral blood leukocytes in vitro by cyclosporine-pretreated human fetal isletlike cell clusters.

  1987cites this paper
• Use of sponge matrix allografts for concurrent monitoring of immunologic and pharmacologic events at a graft site.

  1987cites this paper
• Canine islet autografts with and without administration of cyclosporine.

  1987cites this paper
• Interleukin-2 Effect on the Inhibition of Mised Lymphocyte Reaction Induced by Cyclosporin A

  1987cites this paper
• Novel immunosuppressive agent, FK506. In vitro effects on the cloned T cell activation.

  1987cites this paper
• A longitudinal study of varicella immunity in pediatric renal transplant recipients.

  1986cites this paper
• Cyclosporin. Mechanism of action: in vitro studies.

  1986cites this paper
• Synthesis and antimitogenic activities of four analogues of cyclosporin A modified in the 1-position.

  1986cites this paper
• In vitro effect of cyclosporin A on immunoglobulin production and concanavalin A induced suppression in primary biliary cirrhosis.

  1986cites this paper
• Cyclosporine: progress, problems, and perspectives.

  1986cites this paper
• Prophylactic treatment of BALB/c mice with cyclosporine A and its analog B-5-49 enhances resistance to Leishmania major.

  1986cites this paper
• Supplement of Exogenous IL2 Abrogates Induction of Tolerance in Drug‐Induced T Cell Proliferation

  1986cites this paper
• Characterisation of cyclosporine-induced suppressor cells in murine delayed-type hypersensitivity responses.

  1986cites this paper
• Effects of cyclosporin A, antilymphocyte serum and donor-specific transfusions on murine delayed-type hypersensitivity and skin graft survival.

  1986cites this paper
• [Immunopharmacology of cyclosporin A].

  1986cites this paper
• Cyclosporine: immunology, pharmacology and therapeutic uses.

  1986cites this paper
• Cyclosporine and the immune response: basic aspects.

  1986cites this paper
• PHARMACODYNAMIC ASSESSMENT OF THE IN VIVO CYCLOSPORINE EFFECT ON INTERLEUKIN‐2 PRODUCTION BY LYMPHOCYTES IN KIDNEY TRANSPLANT RECIPIENTS

  1985cites this paper
• Cyclosporine in cardiac transplantation.

  1985cites this paper
• Effect of Cyclosporin and Interleukin‐2 on the Restoration of in Vitro Immune Responses to Cytomegalovirus

  1985cites this paper
• FAILURE OF CYCLOSPORINE TO PREVENT IN VIVO T CELL PRIMING IN MAN: STUDIES IN ALLOGENEIC SPLEEN TRANSPLANTATION

  1985cites this paper
• Recombinant interleukin 2 regulates levels of c-myc mRNA in a cloned murine T lymphocyte

  1985cites this paper
• [Mechanism of action of cyclosporin A].

  1985cites this paper
• Effect of interleukin 2 on the immunosuppressive action of cyclosporine.

  1985cites this paper
• Suppression of in vitro CML generation by alloactivated lymphocytes: analysis of antigen-nonspecific suppressive mechanisms.

  1985cites this paper
• Cyclosporine and murine responses to alloantigens: effects of cyclosporine and donor-specific transfusions on delayed hypersensitivity reactions and murine skin allografts.

  1985cites this paper
• Antigen/mitogen induced cytolytic activity and IL-2 secretion in memory-like CTL-hybridomas.

  1985cites this paper
• Development of graft-vs.-host disease-like syndrome in cyclosporine- treated rats after syngeneic bone marrow transplantation. I. Development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity

  1985cites this paper
• Cyclosporin in autoimmune disease.

  1985cites this paper
• Inhibition of T cell cytotoxicity by cyclosporine (CSA), adenosine (Ado) and an inhibitor of adenosine deaminase (ADA).

  1985cites this paper
• Clinical and experimental studies with cyclosporine in renal transplantation.

  1985cites this paper
• Effect of Ciclosporin (CyA) on Suppressor Cell Function in Patients with Primary Biliary Cirrhosis

  1985cites this paper
• Acquired immune deficiency syndrome: No evidence of the presence of cyclosporine

  1984cites this paper
• Suppressor T cell growth and differentiation. Identification of a cofactor required for suppressor T cell function and distinct from interleukin 2

  1984cites this paper
• [Cyclosporin: experimental studies and clinical use in organ transplants].

  1984cites this paper
• Mechanisms of Rejection and Prolongation of Vascularized Organ Allografts

  1984cites this paper
• Beneficial effect of cyclosporin A on the lymphocytic choriomeningitis virus infection in mice

  1984cites this paper
• Mechanism of action of Cyclosporin A: inhibition of lymphokine secretion studied with antigen-stimulated T cell hybridomas.

  1984cites this paper
• Cyclosporine: a new immunosuppressive agent for organ transplantation.

  1984cites this paper
• Sparing of suppressor cells: a critical action of cyclosporine.

  1984cites this paper
• Induction of alloreactive immunosuppression by 1,4-bis [( 2-aminoethyl)amino]-5,8-dihydroxy-9,10-anthracenedione dihydrochloride (CL 232,468).

  1984cites this paper
• Cyclosporine: Immunology, toxicity and pharmacology in experimental animals

  1984cites this paper
• Rapid loss of sensitivity of mitogen-induced lymphocyte activation to inhibition by cyclosporin A.

  1984cites this paper
• Cyclosporin A inhibits the delayed-type hypersensitivity effector function of T lymphocytes without affecting their clonal expansion.

  1984cites this paper
• THE IMMUNOSUPPRESSIVE MODE OF ACTION OF MIZORIBINE

  1984cites this paper
• IMMUNOPHARMACODYNAMIC EVALUATION OF CYCLOSPORINE‐TREATED RENAL ALLOGRAFT RECIPIENTS

  1984cites this paper
• BLOCKING BY CYCLOSPORINE OF ANTIGEN‐INDUCED MATURATION AND LYMPHOKINE SECRETION BY CYTOTOXIC T LYMPHOCYTE HYBRIDOMAS

  1984cites this paper
• No Evidence of the Presence of Cyclosporine

  1984cites this paper
• The function of effector cells in influenza virus infection

  1984cites this paper
• Mechanism of Action and Clinical Applications of Cyclosporin in Organ Transplantation

  1984cites this paper
• Immunobiology of cyclosporin A--a review.

  1983cites this paper
• Effect of cyclosporine on the response of normal human lymphocytes to cytomegalovirus in vitro

  1983cites this paper
• A POTENTIAL SYNERGISTIC EFFECT OF DONOR ANTIGENIC EXTRACTS AND CYCLOSPORINE ON THE PROLONGATION OF RAT RENAL ALLOGRAFTS

  1983cites this paper
• Effects of cyclosporin A on the metabolism of unstimulated and mitogen-activated lymphocytes.

  1983cites this paper
• Cyclosporine: a new and promising immunosuppressive agent.

  1983cites this paper
• Cyclosporin A inhibits lymphokine production but not the responses of macrophages to lymphokines.

  1983cites this paper
• Effect of cyclosporine on experimental autoimmune thyroiditis in mice.

  1983cites this paper
• Inhibition of the production of a soluble helper mediator by cyclosporin A results in the failure to generate alloreactive cytolytic cells in mixed-lymphocyte culture.

  1983cites this paper
• Binding of cyclosporine by human lymphocytes and phospholipid vesicles.

  1983cites this paper