Suppression subtractive hybridization identified differentially expressed genes in colorectal cancer: microRNA-451a as a novel colorectal cancer–related gene

To investigate differentially expressed genes regulated by microRNA-451a in colorectal cancer. We detected expression of microRNA-451a in colorectal cancer samples and normal pericarcinous tissues from 68 colorectal cancer patients and the correlation between microRNA-451a and clinical features of these patients. Then, the expression of microRNA-451a in HCT116, SW620, HT29, SW480, and DLD cells was also measured. The suppression subtractive hybridization method was used with two HCT116 cell lines with overexpressing or underexpressing microRNA-451a, respectively. The most highly increased genes were screened. Their functions were predicted by gene ontology analysis. The expression ratio of microRNA-451a in colorectal cancer to pericarcinous tissues was 0.37. Expression of microRNA-451a was decreased in HCT116, SW620, HT29, SW480, and DLD cells. In our suppression subtractive hybridization library, expression of seven genes was the most highly increased when underexpressing microRNA-451a. They were BCAP31, EEF1A1, CDC20, WDR6, TUFM, RPL13, and RPL7A. Expression of DKK1, PSME1, NDUFA3, and GNB2 was most highly increased when overexpressing microRNA-451a. Gene ontology analysis showed that the main functions of these genes were associated with translational elongation, protein localization to the endoplasmic reticulum, translation, poly(A) RNA binding, negative regulations of Wnt signaling pathway, and so on. MicroRNA-451a was demonstrated to be downregulated in colorectal cancer patient tissues whose target genes were analyzed and functions were predicted by suppression subtractive hybridization.

• Publication year

  2017

• Venue

  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

• Publication date

  2017-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1177/1010428317705504PMID 28468585
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dual Inhibition of MEK and PI3K Pathway in KRAS and BRAF Mutated Colorectal Cancers

  2015cited by this paper
• Concerted copy number variation balances ribosomal DNA dosage in human and mouse genomes

  2015cited by this paper
• Clinical Validation of Targeted Next Generation Sequencing for Colon and Lung Cancers

  2015cited by this paper
• MicroRNAs in colorectal cancer: small molecules with big functions.

  2015cited by this paper
• The Key Role of Calmodulin in KRAS-Driven Adenocarcinomas

  2015cited by this paper
• MicroRNAs as prognostic molecular signatures in human head and neck squamous cell carcinoma: a systematic review and meta-analysis.

  2015cited by this paper
• Functional Dynamics within the Human Ribosome Regulate the Rate of Active Protein Synthesis.

  2015cited by this paper
• MiR-451, a potential prognostic biomarker and tumor suppressor for gastric cancer.

  2015cited by this paper
• Nucleolar dominance and maternal control of 45S rDNA expression

  2015cited by this paper
• MicroRNA-451: epithelial-mesenchymal transition inhibitor and prognostic biomarker of hepatocelluar carcinoma

  2015cited by this paper
• miR-451 regulates FoxO3 nuclear accumulation through Ywhaz in human colorectal cancer.

  2015cited by this paper
• Multi-Scale Genomic, Transcriptomic and Proteomic Analysis of Colorectal Cancer Cell Lines to Identify Novel Biomarkers

  2015cited by this paper
• Similar but different: distinct roles for KRAS and BRAF oncogenes in colorectal cancer development and therapy resistance

  2015cited by this paper
• Feasibility of urinary microRNA detection in breast cancer patients and its potential as an innovative non-invasive biomarker

  2015cited by this paper
• Roles of microRNAs during prostatic tumorigenesis and tumor progression

  2014cited by this paper
• MicroRNAs in cancer.

  2014cited by this paper
• Molecular biomarkers and classification models in the evaluation of the prognosis of colorectal cancer.

  2014cited by this paper
• A five-microRNA panel in plasma was identified as potential biomarker for early detection of gastric cancer

  2014cited by this paper
• Focus on genetic and epigenetic events of colorectal cancer pathogenesis: implications for molecular diagnosis

  2014cited by this paper
• Suppression subtractive hybridization identified differentially expressed genes in lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene

  2013cited by this paper
• TYMS serves as a prognostic indicator to predict the lymph node metastasis in Chinese patients with colorectal cancer.

  2013cited by this paper
• Roles of Mir-144-ZFX Pathway in Growth Regulation of Non-Small-Cell Lung Cancer

  2013cited by this paper
• Tumor markers in colorectal cancer, gastric cancer and gastrointestinal stromal cancers: European group on tumor markers 2014 guidelines update

  2013cited by this paper
• miRNAs and cancer: an epigenetics view.

  2013cited by this paper
• Colorectal cancer and RASSF family—A special emphasis on RASSF1A

  2013cited by this paper
• Dependency of colorectal cancer on a TGF-β-driven program in stromal cells for metastasis initiation.

  2012cited by this paper
• The Novel WHO 2010 Classification for Gastrointestinal Neuroendocrine Tumours Correlates Well with the Metastatic Potential of Rectal Neuroendocrine Tumours

  2012cited by this paper
• MicroRNA-451 in chronic myeloid leukemia: miR-451-BCR-ABL regulatory loop?

  2011cited by this paper
• Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat

  2011cited by this paper
• Perspectives on current tumor-node-metastasis (TNM) staging of cancers of the colon and rectum.

  2011cited by this paper
• microRNA-451: A conditional switch controlling glioma cell proliferation and migration

  2010cited by this paper
• Loss of ATM positively regulates the expression of hypoxia inducible factor 1 (HIF-1) through oxidative stress: Role in the physiopathology of the disease

  2010cited by this paper
• microRNA-451 Regulates Macrophage Migration Inhibitory Factor Production and Proliferation of Gastrointestinal Cancer Cells

  2009cited by this paper
• Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening

  2009cited by this paper
• MicroRNAs in Cancer.

  2009cited by this paper
• Identification of differential expression of genes in hepatocellular carcinoma by suppression subtractive hybridization combined cDNA microarray.

  2007cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.

  1996cited by this paper
• Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method

  year unknowncited by this paper

• Shikonin Induces Glioma Necroptosis, Stemness Decline, and Impedes (Immuno)Proteasome Activity

  2024cites this paper
• TUFM in health and disease: exploring its multifaceted roles

  2024cites this paper
• Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression

  2023cites this paper
• CAR T Cell Therapy: Remedies of Current Challenges in Design, Injection, Infiltration and Working

  2023cites this paper
• Ontologies and Knowledge Graphs in Oncology Research

  2022cites this paper
• A two-microRNA signature predicts the progression of male thyroid cancer

  2021cites this paper
• Comprehensive Analysis of Prognostic and Genetic Signatures for General Transcription Factor III (GTF3) in Clinical Colorectal Cancer Patients Using Bioinformatics Approaches

  2021cites this paper
• Molecular subtype identification and prognosis stratification by a metabolism-related gene expression signature in colorectal cancer

  2021cites this paper
• Expression Analysis of Ribosomal Protein Genes, eL13 and eL14 in Nasopharyngeal Carcinoma Cell Lines

  2020cites this paper
• Long intergenic non-protein coding RNA-467 targets microRNA-451a in human colorectal cancer

  2020cites this paper
• Upregulation of cell division cycle 20 in cisplatin resistance-induced epithelial-mesenchymal transition in osteosarcoma cells.

  2020cites this paper
• MiR-451a suppressing BAP31 can inhibit proliferation and increase apoptosis through inducing ER stress in colorectal cancer

  2019cites this paper
• Neuroevolution as a Tool for Microarray Gene Expression Pattern

  year unknowncites this paper