Human polymorphonuclear leukocytes (PMN) are able to mediate the immunologically specific cytolysis of antibody-coated target cells—antibody-dependent cellular cytotoxicity (ADCC). The initial interaction is divalent cation dependent and is inhibited by ethylenediaminetetraacetic acid (EDTA). Agents capable of elevating adenosine cyclic 3′,5′ monophosphate (cyclic 3′,5′ AMP) and exogenous cyclic 3′,5′ AMP are inhibitory to PMN-mediated ADCC activity, while cholinergic agents capable of elevating guanosine 3′,5′ cyclic monophosphate (cyclic 3′,5′ GMP) and exogenous cyclic 3′,5′ GMP exhibit a facilitory effect. Colchicine in concentrations inhibitory to microtubule formation and pharmacologic agents capable of interfering with granule release are inhibitory to PMN ADCC activity.
Modulation of polymorphonuclear leukocyte-mediated antibody-dependent cellular cytotoxicity.
Published 1974 in Journal of Immunology
ABSTRACT
PUBLICATION RECORD
- Publication year
1974
- Venue
Journal of Immunology
- Publication date
1974-12-01
- Fields of study
Biology, Medicine, Chemistry
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- antibody-coated target cells
Target cells bearing bound antibody that are attacked in the cytotoxicity assay.
Aliases: antibody-coated cells
- antibody-dependent cellular cytotoxicity (adcc)
An immune effector process in which leukocytes lyse target cells that have been coated with antibody.
Aliases: ADCC
- cholinergic agents
Compounds that act on cholinergic signaling and were tested for their effect on PMN ADCC.
Aliases: cholinergic drugs
- colchicine
A microtubule-disrupting drug examined for its effect on PMN-mediated cytotoxicity.
- cyclic amp
A cyclic nucleotide second messenger considered here as a modulator of PMN ADCC.
Aliases: cAMP, adenosine cyclic 3′,5′ monophosphate
- cyclic gmp
A cyclic nucleotide second messenger considered here as a modulator of PMN ADCC.
Aliases: cGMP, guanosine 3′,5′ cyclic monophosphate
- divalent cations
Two-valent ions required for the early PMN-target interaction in this assay.
Aliases: divalent cation
- edta
A chelating reagent used to sequester divalent cations and block cation-dependent interactions.
Aliases: ethylenediaminetetraacetic acid
- granule release
The exocytotic discharge of PMN granule contents during the cytotoxic response.
Aliases: granule secretion
- polymorphonuclear leukocytes (pmn)
A class of granulocytic leukocytes, mainly neutrophils, used here as the effector cells in the cytotoxicity assay.
Aliases: PMN, polymorphonuclear leukocytes
REFERENCES
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