Helical Structure and Packing Orientation of the S2 Segment in the Shaker K+ Channel

Six transmembrane segments, S1–S6, cluster around the central pore-forming region in voltage-gated K+ channels. To investigate the structural characteristics of the S2 segment in the Shaker K+ channel, we replaced each residue in S2 singly with tryptophan (or with alanine for the native tryptophan). All but one of the 23 Trp mutants expressed voltage-dependent K+ currents in Xenopus oocytes. The effects of the mutations were classified as being of low or high impact on channel gating properties. The periodicity evident in the effects of these mutations supports an α-helical structure for the S2 segment. The high- and low-impact residues cluster onto opposite faces of a helical wheel projection of the S2 segment. The low-impact face is also tolerant of single mutations to asparagine. All results are consistent with the idea that the low-impact face projects toward membrane lipids and that changes in S2 packing occur upon channel opening. We conclude that the S2 segment is a transmembrane α helix and that the high-impact face packs against other transmembrane segments in the functional channel.

• Publication year

  1999

• Venue

  The Journal of General Physiology

• Publication date

  1999-03-01

• Fields of study

  Biology, Medicine, Physics

• Identifiers
  DOI 10.1085/JGP.113.3.415PMID 10051517PMCID 2222899
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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