Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9

Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use the RNA-guided Cas9 nuclease to efficiently generate knockouts without selection, and to introduce point mutations and epitope tags into the T. gondii genome. These methods will streamline the functional analysis of parasite genes and enable high-throughput engineering of their genomes.

• Publication year

  2014

• Venue

  PLoS ONE

• Publication date

  2014-06-27

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1371/journal.pone.0100450PMID 24971596PMCID 4074098
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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