Synthetic shRNAs as potent RNAi triggers

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Nature Biotechnology

• Publication date

  2005-02-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/nbt1052PMID 15619616
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy

  2005cited by this paper
• Single processing center models for human Dicer and bacterial RNase III.

  2004cited by this paper
• Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways.

  2004cited by this paper
• Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity

  2004cited by this paper
• A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila.

  2004cited by this paper
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• Argonaute2 Is the Catalytic Engine of Mammalian RNAi

  2004cited by this paper
• Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain

  2004cited by this paper
• Nuclear Export of MicroRNA Precursors

  2004cited by this paper
• RISC assembly defects in the Drosophila RNAi mutant armitage.

  2004cited by this paper
• Asymmetry in the assembly of the RNAi enzyme complex.

  2003cited by this paper
• Functional siRNAs and miRNAs exhibit strand bias.

  2003cited by this paper
• Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain

  2003cited by this paper
• The nuclear RNase III Drosha initiates microRNA processing

  2003cited by this paper
• Asymmetry in the Assembly of the RNAi Enzyme Complex

  2003cited by this paper
• Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors.

  2003cited by this paper
• The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes

  2003cited by this paper
• Expression profiling reveals off-target gene regulation by RNAi

  2003cited by this paper
• Structure and conserved RNA binding of the PAZ domain

  2003cited by this paper
• A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

  2002cited by this paper
• Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells.

  2002cited by this paper
• Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP

  2002cited by this paper
• Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.

  2002cited by this paper
• Role for a bidentate ribonuclease in the initiation step of RNA interference

  2001cited by this paper
• Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi

  2001cited by this paper
• A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA

  2001cited by this paper
• Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

  2001cited by this paper
• Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.

  2001cited by this paper
• An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

  2000cited by this paper
• RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.

  2000cited by this paper

• DICER cleavage fidelity is governed by 5'-end binding pockets.

  2026cites this paper
• DsiRNA-mediated silencing of Ceratitis capitata transformer or transformer-2 leads to masculinization of XX embryos and systemic gene silencing in ovaries.

  2025cites this paper
• Multifunctional Small RNA Endogenous Modified Exosome‐Loaded Hydrogel Based on Photocrosslinking for Cartilage Repair

  2025cites this paper
• shRNAI: A deep neural network for the design of highly potent shRNAs

  2025cites this paper
• Analysis of Processing, Post-Maturation, and By-Products of shRNA in Gene and Cell Therapy Applications

  2025cites this paper
• Gene Therapy for Efficient Suppression of T-Type Channels in Treating Diabetic Neuropathy

  2025influential citation
• Pharmaceutical perspectives on oligonucleotide therapeutics and delivery systems.

  2025cites this paper
• The Growing Class of Novel RNAi Therapeutics

  2024cites this paper
• Challenges of Spatially Resolved Metabolism in Cancer Research

  2024cites this paper
• Direct observation of cytoskeleton-dependent trafficking of miRNA visualized by the introduction of pre-miRNA

  2024cites this paper
• Single-Stranded Hairpin Loop RNAs (loopmeRNAs) Potently Induce Gene Silencing through the RNA Interference Pathway.

  2024cites this paper
• On the ever‐growing functional versatility of the CRISPR‐Cas13 system

  2024cites this paper
• Determinants of selectivity in the dicing mechanism

  2024cites this paper
• Two-motif model illuminates DICER cleavage preferences

  2024cites this paper
• The emerging roles of long noncoding RNAs in lymphatic vascular development and disease

  2023cites this paper
• A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicus

  2023cites this paper
• Mfap4: a promising target for enhanced liver regeneration and chronic liver disease treatment

  2023cites this paper
• Terminal bridging of siRNA duplex at the ribose 2′ position controls strand bias and target sequence preference

  2023cites this paper
• Secondary structure RNA elements control the cleavage activity of DICER

  2022cites this paper
• An Artificial Small RNA Editor by Chimeric dsRNase with RNA Binding Protein

  2022cites this paper
• MicroRNAs: master regulators in host–parasitic protist interactions

  2022influential citation
• VEGFA-targeting miR-agshRNAs combine efficacy with specificity and safety for retinal gene therapy

  2022cites this paper
• RNA interference activity of single-stranded oligonucleotides linked between the passenger strand and the guide strand with an aryl phosphate linker

  2021cites this paper
• 14‐3‐3β isoform is specifically acetylated at Lys51 during differentiation to the osteogenic lineage

  2021cites this paper
• Adeno-Associated Virus Vector for Central Nervous System Gene Therapy.

  2021cites this paper
• Toward the therapeutic application of small interfering RNA bioconjugates in the central nervous system

  2020cites this paper
• An RNAi therapeutic, DFP-10825, for intraperitoneal and intrapleural malignant cancers.

  2020cites this paper
• Prospects for the potential of RNA interference in the treatment of autoimmune diseases: Small interfering RNAs in the spotlight.

  2020cites this paper
• Retraction Note to: The Semaphorin 4D-Plexin-B1-RhoA signaling axis recruits pericytes and regulates vascular permeability through endothelial production of PDGF-B and ANGPTL4

  2020cites this paper
• Going wild for functional genomics: RNA interference as a tool to study gene-behavior associations in diverse species and ecological contexts.

  2020cites this paper
• The role of MAPRE2 as a potential key gene in leukemic stem cells

  2020cites this paper
• Computational insights into RNAi-based therapeutics for foot and mouth disease of Bostaurus

  2020cites this paper
• RNA Interference Technology

  2019cites this paper
• Editorial focus: understanding off-target effects as the key to successful RNAi therapy

  2019cites this paper
• Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors.

  2019cites this paper
• Enhancement of gene knockdown efficiency by CNNC motifs in the intronic shRNA precursor

  2019cites this paper
• Decoding non-coding RNAs in fatty liver disease

  2019cites this paper
• Antiviral RNAi in Insects and Mammals: Parallels and Differences

  2019cites this paper
• Hyaluronic Acid as an Emerging Technology Platform for Silencing RNA Delivery

  2019cites this paper
• Design, mechanism, delivery and therapeutics of canonical and Dicer-substrate siRNA

  2019influential citation
• GENE THERAPY FOR AMYOTROPHIC LATERAL SCLEROSIS: AN AAV MEDIATED RNAi APPROACH FOR AUTOSOMAL DOMINANT C9ORF72 ASSOCIATED ALS

  2019cites this paper
• Enhancement of gene knockdown efficiency by CNNC motifs in the intronic shRNA precursor

  2019cites this paper
• Epitranscriptomics.

  2019cites this paper
• The Application of the RNA Interference Technologies for KRAS: Current Status, Future Perspective and Associated Challenges.

  2019cites this paper
• An investigation into the role of mesoaccumbal GABAA receptor α2 subunit in mediating cocaine-facilitated conditioned behaviours using the RNA interference system

  2019cites this paper
• Nanotechnology-based siRNA Delivery Strategies for Metastatic Colorectal Cancer Therapy.

  2019cites this paper
• Influence of a 3′ Terminal Ribozyme on AgoshRNA Biogenesis and Activity

  2019cites this paper
• A multifunctional toolkit for target-directed cancer therapy.

  2019cites this paper
• A RNA producing DNA hydrogel as a platform for a high performance RNA interference system

  2018cites this paper
• Recent Development of Chemical Synthesis of RNA

  2018cites this paper
• DROSHA Knockout Leads to Enhancement of Viral Titers for Vectors Encoding miRNA-Adapted shRNAs

  2018cites this paper
• 3′ RNA Uridylation in Epitranscriptomics, Gene Regulation, and Disease

  2018cites this paper
• Slicing Chapter 2 Biogenesis and Physiology of MicroRNAs

  2018cites this paper
• Extracellular nucleic acids of the marine bacterium Rhodovulum sulfidophilum and recombinant RNA production technology using bacteria

  2018cites this paper
• Targeting metastatic colorectal cancer with PLGA nanoparticles carrying MACC1 siRNA

  2018cites this paper
• Fundamentals of siRNA and miRNA therapeutics and a review of targeted nanoparticle delivery systems in breast cancer

  2018cites this paper
• Improving miRNA Delivery by Optimizing miRNA Expression Cassettes in Diverse Virus Vectors

  2017cites this paper
• RNA interference for glioblastoma therapy: Innovation ladder from the bench to clinical trials.

  2017cites this paper
• United States Patent Shoshan-Barmatz

  2017cites this paper
• Biodegradable nano-polymers as delivery vehicles for therapeutic small non-coding ribonucleic acids.

  2017cites this paper
• Chapter 5 Noncanonical Synthetic RNAi Inducers

  2017influential citation
• Induction of RNAi Responses by Short Left-Handed Hairpin RNAi Triggers

  2017cites this paper
• Dicer-independent processing of small RNA duplexes: mechanistic insights and applications

  2017influential citation
• Noncanonical Synthetic RNAi Inducers

  2016influential citation
• Engineering Periodic shRNA for Enhanced Silencing Efficacy.

  2016cites this paper
• Design of shRNA and miRNA for Delivery to the CNS.

  2016cites this paper
• shRNAs: A few keypoints about their applications

  2016cites this paper
• Chemical and structural modifications of RNAi therapeutics.

  2016cites this paper
• Guidelines for the optimal design of miRNA-based shRNAs.

  2016cites this paper
• Gene and virotherapy for hematological malignancies

  2016cites this paper
• Tospovirus : induction and suppression of RNA silencing

  2016cites this paper
• Inhibitory effects of shRNA on expression of JNK1 and migration and invasion in mouse hepatocellular carcinoma cell lines mediated by ultrasound-targeted microbubble destruction.

  2016cites this paper
• Gene editing in T-cells and T-cell targets

  2016cites this paper
• Viral RNA Silencing Suppression: The Enigma of Bunyavirus NSs Proteins

  2016cites this paper
• Penetration and Silencing Activity of VEGF Dicer Substrate siRNA Vectorized by Chitosan Nanoparticles in Monolayer Culture and a Solid Tumor Model In Vitro for Potential Application in Tumor Therapy

  2016cites this paper
• Circular Oligodeoxynucleotides (Coligos) as Chemically Synthesized, Promoter-Independent Small RNA Expression Vectors

  2016influential citation
• Promyelocytic leukaemia protein isoform II and mild heat stress compromise human adenovirus type 5 gene expression

  2016cites this paper
• Semaphorin 4D Promotes Skeletal Metastasis in Breast Cancer

  2016cites this paper
• RNA INTERFERENCE IN DENTISTRY: A CRITICAL REVIEW

  2016cites this paper
• Design and characterization of new classes of antagonists targeting the Lin28/let-7 interaction

  2015cites this paper
• Expression of anti-viral shRNA molecules in transgenic zebrafish

  2015cites this paper
• RNA Interference Using c-Myc–Conjugated Nanoparticles Suppresses Breast and Colorectal Cancer Models

  2015cites this paper
• Triggering RNAi with multifunctional RNA nanoparticles and their delivery

  2015cites this paper
• Effective Inhibition of HIV-1 Production by Short Hairpin RNAs and Small Interfering RNAs Targeting a Highly Conserved Site in HIV-1 Gag RNA Is Optimized by Evaluating Alternative Length Formats

  2015cites this paper
• Toward optimization of AgoshRNA moleculesthat use a non-canonical RNAi pathway: Variations in the top and bottom base pairs

  2015cites this paper
• MicroRNAs 2 . 1 . 1 Defi nition and Genomic Organization

  2015cites this paper
• Immunological and hematological toxicities challenging clinical translation of nucleic acid-based therapeutics

  2015cites this paper
• UvA-DARE ( Digital Academic Repository ) HIV-1 in the RNA world : Transcription regulation , miRNAs and antiviral RNAs

  2015cites this paper
• Development of a microRNA Delivery Scaffold System for Bone Tissue Engineering

  2015cites this paper
• Gene therapy strategies to block HIV-1 replication by RNA interference.

  2015cites this paper
• Comparing the silencing efficacy of dicer-independent and dependent shRNAs

  2015influential citation
• G‐protein–coupled receptor 137 accelerates proliferation of urinary bladder cancer cells in vitro

  2015cites this paper
• Identification of novel chromatin and nuclear factors regulating oncogene-induced senescence

  2015cites this paper
• Molecular Properties, Functional Mechanisms, and Applications of Sliced siRNA

  2015cites this paper
• siRNA Versus miRNA as Therapeutics for Gene Silencing

  2015cites this paper
• Small RNAs and Regulation of Gene Expression in Entamoeba histolytica

  2015cites this paper
• Efficacious gene silencing in serum and significant apoptotic activity induction by survivin downregulation mediated by new cationic gemini tocopheryl lipids.

  2015cites this paper
• Current Prospects of RNA Interference-based Therapy in Organ Transplantation

  2015cites this paper
• RNA interference approaches for treatment of HIV-1 infection

  2015cites this paper
• Subtle Controllers: MicroRNAs Drive Pancreatic Tumorigenesis and Progression: A Dissertation

  2015cites this paper