Comparison of chemical cleaning methods of glass in preparation for silanization

The uniform deposition of a silane monolayer upon glass has been shown to require small amounts of water along with hydroxyl groups in an isolated or geminal configuration on the substrate surface. In order to expose these groups, organic compounds at the surface must be removed. We present a qualitative evaluation of eight chemical methods commonly used to clean glass microscope slides in preparation for silanization. Mean contact angle of deionized water was measured before covalent attachment of (3-mercaptopropyl)triethoxysilane to assess the efficacy of each procedure. Contact angles were also measured after silanization as a means of determining the uniformity and reproducibility of the silane monolayer. The results indicate that a 1:1 methanol/HC1 wash followed by a bath in concentrated H2SO4 removes surface contaminants most effectively and allows for a very even silanization of the glass surface.

• Publication year

  1999

• Venue

  Biosensors and Bioelectronics

• Publication date

  1999-12-01

• Fields of study

  Materials Science, Chemistry

• Identifiers
  DOI 10.1016/S0956-5663(99)00043-3
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Immobilized biomolecules in analysis : a practical approach

  1998cited by this paper
• Functionalized Monolayers on Ordered Mesoporous Supports

  1997cited by this paper
• Silylation of the silica surface a review

  1996cited by this paper
• Cysteine-specific surface tethering of genetically engineered cytochromes for fabrication of metalloprotein nanostructures

  1994cited by this paper
• Characterization of silica surfaces hydrophobized by octadecyltrichlorosilane

  1994cited by this paper
• Covalent immobilization of protein monolayers for biosensor applications.

  1994cited by this paper
• Fabrication of surfaces resistant to protein adsorption and application to two-dimensional protein patterning.

  1993cited by this paper
• Effects of surface hydration on the deposition of silane monolayers on silica

  1993cited by this paper
• Horizontal polymerization of mixed trifunctional silanes on silica. 2. Application to chromatographic silica gel

  1993cited by this paper
• Surface reactions on thin layers of silane coupling agents

  1993cited by this paper
• Horizontal polymerization of mixed trifunctional silanes on silica: a potential chromatographic stationary phase

  1992cited by this paper
• Etching roughness for (100) silicon surfaces in aqueous KOH

  1991cited by this paper
• Silica surface controversies, strong adsorption sites, their blockage and removal. Part II

  1991cited by this paper
• Silanation of silica surfaces. A new method of constructing pure or mixed monolayers

  1991cited by this paper
• Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold

  1989cited by this paper
• Surface immobilization techniques in combination with ellipsometry.

  1988cited by this paper
• Kinetics of titanium silicide formation on single‐crystal Si: Experiment and modeling

  1988cited by this paper
• Ellipsometric Study of Orientation‐Dependent Etching of Silicon in Aqueous KOH

  1985cited by this paper
• Immobilization of immunoglobulins on silica surfaces. Stability.

  1985cited by this paper
• Covalently attached organic monolayers on semiconductor surfaces

  1978cited by this paper
• [10] Covalent coupling methods for inorganic support materials

  1976cited by this paper
• Cleaning solutions based on hydrogen peroxide for use in silicon semiconductor technology

  1970cited by this paper
• Clean Surfaces: Their Preparation and Characterization for Interfacial Studies

  1970cited by this paper
• Thermodynamic Properties of Adsorbed Water Molecules and Electrical Conduction in Montmorillonites and Silicas

  1965cited by this paper
• The Status of Contact Angle as a Thermodynamic Property

  1964cited by this paper
• Contact Angle Hysteresis: II. Contact Angle Measurements on Rough Surfaces

  1964cited by this paper

• "Silatranization": Surface modification with silatrane coupling agents.

  2025cites this paper
• Interpolymer Complexation Between Cellulose Ethers, Poloxamers, and Polyacrylic Acid: Surface-Dependent Behavior

  2025cites this paper
• Development of a High-Performance Immunoaffinity Separation Technique for Rare Cell Capture

  2025cites this paper
• Optimized reconstruction of adhesion force distribution from resuspension measurements using the Rock’n’Roll model

  2025cites this paper
• Improvement of the optical cavity-based biosensor’s limit of detection using optimal 3-aminopropyltriethoxysilane process

  2025influential citation
• Cross-Limit PID Control Strategy Simulator with Transfer Functions Support, Integrating Siemens S7-1512 PLC, MATLAB and InduSoft Web Studio

  2025cites this paper
• Eliminating adhesive layers in silver metallization: A comparative study of glass cleaning methods for enhanced hydroxylation and adhesion

  2025cites this paper
• Nanoscale analysis of surface modifications on silanized glass: Wettability alteration and long-term stability

  2025cites this paper
• Development of TiO2 Films by Sol–Gel/Sedimentation for the Inactivation of Multidrug-Resistant Escherichia coli and Salmonella Typhimurium in Greywater

  2025cites this paper
• How Surface and Substrate Chemistry Affect Slide Electrification

  2024cites this paper
• Probing G-Quadruplexes Conformational Dynamics and Nano-Mechanical Interactions at the Single Molecule Level: Techniques and Perspectives

  2024cites this paper
• Adsorbed polymer conjugates to adaptively inhibit blood coagulation activation by medical membranes.

  2024cites this paper
• Hydrophobicity and membrane distillation performance of glass fiber membranes modified by dip coating of pure PDMS

  2024cites this paper
• Glyphosate Detection Through Piezoelectric and Fiber Optic Sensors Based on Molecular Imprinted Polymers

  2024cites this paper
• Microbead-based extracorporeal immuno-affinity virus capture: a feasibility study to address the SARS-CoV-2 pandemic

  2023cites this paper
• Dynamic matrices with DNA-encoded viscoelasticity for cell and organoid culture

  2023cites this paper
• Systematic Study of Wettability Alteration of Glass Surfaces by Dichlorooctamethyltetrasiloxane Silanization—A Guide for Contact Angle Modification

  2023cites this paper
• Optimization of platelet-rich fibrin.

  2023cites this paper
• Controlled Assembly of CdSe Nanoplatelet Thin Films and Nanowires

  2023cites this paper
• The role of mixing and surface hydrophobicity on the operation of a continuous tubular slug flow crystalliser for lysozyme

  2023cites this paper
• Targeting Insect Olfaction in vivo and in vitro Using Functional Imaging

  2022cites this paper
• Synthesis of Superhydrophilic Gradient-Like Copolymers: Kinetics of the RAFT Copolymerization of Methacryloyloxyethyl Phosphorylcholine with PEO Methacrylate

  2022cites this paper
• Kinetic, Isotherm, and Equilibrium Investigation of Cr(VI) Ion Adsorption on Amine-Functionalized Porous Silica Beads

  2022cites this paper
• Solution deposition conditions influence the surface properties of 3-mercaptopropyl(trimethoxysilane) (MPTS) films

  2022cites this paper
• Detection of Tomato Leaf Curl New Delhi Virus DNA Using U-Bent Optical Fiber-Based Lspr Probes

  2022cites this paper
• Review: 3-Aminopropyltriethoxysilane (APTES) Deposition Methods on Oxide Surfaces in Solution and Vapor Phases for Biosensing Applications

  2022cites this paper
• Investigation of optical, structural, and electrical properties of heterostructure Fe2O3 deposited by RF magnetron sputtering on ZnO layer by spray pyrolysis

  2021influential citation
• A contribution to wettability and wetting characterisation of ultrafine particles with varying shape and degree of hydrophobization

  2021cites this paper
• Photodegradation of tebuconazole mediated by a novel hybrid phenalenone based photosensitizer

  2021cites this paper
• Potential applications of nanoparticles embedded U-bent fiber optic probe

  2021cites this paper
• Synthesis of hydrophobic fluorinated coating by further addition of isocyanate and amine groups to urethane bond

  2021cites this paper
• Absorbance-based detection of arsenic in a microfluidic system with push-and-pull pumping

  2021cites this paper
• Hydroxylation methods for mesoporous silica and their impact on surface functionalisation

  2021cites this paper
• Effect of surface wettability on the interfacial adhesion of a thermosetting elastomer on glass

  2021cites this paper
• Combinations of dual-function azide-containing crosslinkers with C-H insertion capabilities for polymeric elastomers with improved adhesion properties

  2021cites this paper
• Control of glass surface wettability via esterification with n-alkyl alcohols

  2021cites this paper
• Assessment of nanoparticle immersion depth at liquid interfaces from chemically equivalent macroscopic surfaces.

  2021cites this paper
• Modification of the glass surface by DCSBD plasma discharge to improve adhesion of decorative gold

  2021cites this paper
• Controlled deposition of nanoparticles with critical Casimir forces

  2021cites this paper
• A versatile mitochondria isolation- and analysis-pipeline generates 3D nano-topographies and mechano-physical surface maps of single organelles

  2021cites this paper
• Tip Recycling for Atomic Force Microscopy-Based Tip-Enhanced Raman Spectroscopy

  2020cites this paper
• Studies on surface modification of polypropylene composite bipolar plates using an electroless deposition technique

  2020cites this paper
• Mechanically durable biomimetic fibrous membrane with superhydrophobicity and superoleophilicity for aqueous oil separation

  2020cites this paper
• Comparative study of optimised molybdenum back-contact deposition with different barriers (Ti, ZnO) on stainless steel substrate for flexible solar cell application

  2020cites this paper
• Tree water transport mediating the changing environmental conditions to tree physiological processes

  2020cites this paper
• Monolithic and self-roughened Janus fibrous membrane with superhydrophilic/omniphobic surface for robust antifouling and antiwetting membrane distillation

  2020cites this paper
• Is Decreased Xylem Sap Surface Tension Associated With Embolism and Loss of Xylem Hydraulic Conductivity in Pathogen-Infected Norway Spruce Saplings?

  2020cites this paper
• Studies on high refractive index amorphous TiO2 thin film for possible improvement of light extraction efficiency in organic light emitting diodes

  2020cites this paper
• Superhydrophobic-omniphobic membrane with anti-deformable pores for membrane distillation with excellent wetting resistance

  2020cites this paper
• Tailored Interfaces in Fiber-Reinforced Elastomers: A Surface Treatment Study on Optimized Load Coupling via the Modified Fiber Bundle Debond Technique

  2020cites this paper
• Relationship between local damage and macroscopic response of soft materials highly reinforced by monodispersed particles

  2020cites this paper
• Comparison of the finite strain macroscopic behavior and local damage of a soft matrix highly reinforced by spherical or polyhedral particles

  2020cites this paper
• Total Internal Reflection Transient Absorption Microscopy: An On-Line Detection Method for Microfluidics.

  2020cites this paper
• Surface Preparation: Some Techniques

  2020cites this paper
• A Study in Molecular Recognition: Synthesis of a Β-sheet Mimic & Quantitation of Metal Ions in Aqueous Solutions Through Solid Supported Semi-selective Chemosensors

  2019cites this paper
• Lanthanide-Oligomeric Brush Films: From Luminescence Properties to Structure Resolution

  2019cites this paper
• Mixing of Bidisperse Cohesive Granular Materials in Food Processes

  2019cites this paper
• Conical microstructuring of titanium by reactive gas assisted laser texturing

  2019cites this paper
• A STUDY OF THREE-PHASE FRACTURING IN GRANULAR MEDIA USING HIGH-SPEED IMAGING

  2019cites this paper
• Study and realization of optical sensors based on gold nanostructures

  2019cites this paper
• Controlling self-assembly of ultra-small silver nanoparticles: Surface enhancement of Raman and fluorescent spectra

  2019cites this paper
• Gold Nanoparticle Probe-Assisted Antigen-Counting Chip Using SEM.

  2019cites this paper
• Antibacterial application of covalently immobilized photosensitizers on a surface.

  2019cites this paper
• Cohesion-driven mixing and segregation of dry granular media

  2019cites this paper
• Mesoporous Silica SBA-15 Particles in a Detergent Solution as Abrasive and Coating Material for Household Care Cleaning Products

  2019cites this paper
• Development of rapid and real-time detection of pathogenic E. coli bacteria using microcavity in-line Mach-Zehnder interferometer (μIMZI)

  2019cites this paper
• UvA-DARE ( Digital Academic Repository ) Assembling nanocrystal superstructures

  2019cites this paper
• Microfluidic quantification and separation of yeast based on surface adhesion.

  2019cites this paper
• Glass-ceramics for cancer treatment: So close, or yet so far?

  2019cites this paper
• Hydrogel-based pH-sensors : Development and characterisation of optical and electrical pH sensors based on stimuli-responsive hydrogels

  2019cites this paper
• Derivatisation of nerve agent degradants without removal of water

  2019cites this paper
• Analyzing the antiseptic capacity of silver-functionalized poly(ethylene glycol)-heparin hydrogels after human whole blood exposure.

  2018cites this paper
• Multifunctional Polystyrene Core/Silica Shell Microparticles with Antifouling Properties for Bead-Based Multiplexed and Quantitative Analysis.

  2018cites this paper
• Collagen as Coating Material for 45S5 Bioactive Glass-Based Scaffolds for Bone Tissue Engineering

  2018cites this paper
• Live E. coli bacteria label-free sensing using a microcavity in-line Mach-Zehnder interferometer

  2018cites this paper
• Dual-Bioinspired Design for Constructing Membranes with Superhydrophobicity for Direct Contact Membrane Distillation.

  2018cites this paper
• Electronic Interfaces for Bacteria-Based Biosensing

  2018cites this paper
• Étude et optimisation des interfaces fibre-matrice polymère de composites structuraux à base thermoplastique

  2018cites this paper
• Rapid detection of Escherichia coli in field based potable water applications

  2018influential citation
• Distribution of fluoroalkylsilanes in hydrophobic hybrid sol–gel coatings obtained by co-condensation

  2018cites this paper
• The roles of contact time and contact pressure on the coalescence of water droplets suspended in concentrated bitumen solutions

  2018cites this paper
• White paper on the future of plasma science for optics and glass

  2018cites this paper
• Dye-sensitised nanostructured TiO2 for high sensitivity photodetectors

  2018cites this paper
• Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy

  2018cites this paper
• Glass wool: a novel support for heterogeneous catalysis† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc02115e

  2018cites this paper
• Produção e caracterização de filmes finos transparentes e condutores de óxido de grafeno reduzido

  2018cites this paper
• Sample Injection Techniques.

  2018cites this paper
• Investigation of the photocatalytic activity of titanium dioxide films under visible light measured by electrospray mass spectrometry

  2018cites this paper
• Tissue embedding in a silica hydrogel for functional investigations

  2018influential citation
• Development and evaluation of an IgY based silica matrix immunoassay platform for rapid onsite SEB detection

  2018cites this paper
• Slip Length Dependent Propulsion Speed of Catalytic Colloidal Swimmers near Walls.

  2018cites this paper
• Transparent, abrasion-insensitive superhydrophobic coatings for real-world applications

  2017cites this paper
• P(NIPAAM–co‐HEMA) thermoresponsive hydrogels: an alternative approach for muscle cell sheet engineering

  2017cites this paper
• Analytical Protein Microarrays: Advancements Towards Clinical Applications

  2017cites this paper
• Characterization Of Octadecyltrichlorosilane Self-Assembled Multilayers On Pyrex Glass

  2017cites this paper
• Paper-Based for Isolation of Extracellular Vesicles.

  2017cites this paper
• A fast affinity extraction methodology for rapid screening of bioactive compounds specifically binding to beta2-adrenergic receptor from Xie-Bai-San

  2017influential citation
• Metasurfaces and Colloidal Suspensions Composed of 3D Chiral Si Nanoresonators

  2017cites this paper
• Cheap non-toxic non-corrosive method of glass cleaning evaluated by contact angle, AFM, and SEM-EDX measurements

  2017cites this paper
• Characterization of Cross-linking Vehicle Effect on Surface Interactions between pH-sensitive Drug Delivery Vehicle and Mucin Layers by Colloid Probe Atomic Force Microscopy

  2017influential citation