C/EBPβ Reprograms White 3T3-L1 Preadipocytes to a Brown Adipocyte Pattern of Gene Expression*

cAMP-dependent protein kinase induction of PPARγ coactivator-1α (PGC-1α) and uncoupling protein 1 (UCP1) expression is an essential step in the commitment of preadipocytes to the brown adipose tissue (BAT) lineage. We studied the molecular mechanisms responsible for differential expression of PGC-1α in HIB1B (BAT) and 3T3-L1 white adipose tissue (WAT) precursor cell lines. In HIB1B cells PGC-1α and UCP1 expression is cAMP-inducible, but in 3T3-L1 cells, expression is reduced and is cAMP-insensitive. A proximal 264-bp PGC-1α reporter construct was cAMP-inducible only in HIB1B cells and was suppressed by site-directed mutagenesis of the proximal cAMP response element (CRE). In electrophoretic mobility shift assays, the transcription factors CREB and C/EBPβ, but not C/EBPα and C/EBPδ, bound to the CRE on the PGC-1α promoter region in HIB1B and 3T3-L1 cells. Chromatin immunoprecipitation studies demonstrated that C/EBPβ and CREB bound to the CRE region in HIB1B and 3T3-L1 cell lysates. C/EBPβ expression was induced by cAMP only in HIB1B cells, and overexpression of C/EBPβ rescued cAMP-inducible PGC-1α and UCP1 expression in 3T3-L1 cells. These data demonstrate that differentiation of preadipocytes toward the BAT rather than the WAT phenotype is controlled in part by the action of C/EBPβ on the CRE in PGC-1α proximal promoter.

• Publication year

  2007

• Venue

  Journal of Biological Chemistry

• Publication date

  2007-08-24

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M703101200PMID 17584738
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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