Molecular basis for target RNA recognition and cleavage by human RISC.

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Cell

• Publication date

  2007-07-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/J.CELL.2007.04.037PMID 17632058
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Potent effect of target structure on microRNA function

  2007cited by this paper
• Recapitulation of short RNA-directed translational gene silencing in vitro.

  2006cited by this paper
• Control of translation and mRNA degradation by miRNAs and siRNAs.

  2006cited by this paper
• Perfect seed pairing is not a generally reliable predictor for miRNA-target interactions

  2006cited by this paper
• Cleavage of the siRNA passenger strand during RISC assembly in human cells

  2006cited by this paper
• Designing siRNA That Distinguish between Genes That Differ by a Single Nucleotide

  2006cited by this paper
• Inducible DNA‐loop formation blocks transcriptional activation by an SV40 enhancer

  2005influential reference
• Structural basis for 5′-end-specific recognition of guide RNA by the A. fulgidus Piwi protein

  2005cited by this paper
• Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells

  2005cited by this paper
• Slicer function of Drosophila Argonautes and its involvement in RISC formation.

  2005cited by this paper
• Perspective: machines for RNAi.

  2005influential reference
• MicroRNAs control translation initiation by inhibiting eukaryotic initiation factor 4E/cap and poly(A) tail function.

  2005cited by this paper
• Local RNA target structure influences siRNA efficacy: a systematic global analysis.

  2005cited by this paper
• Uncoupling of RNAi from active translation in mammalian cells.

  2005cited by this paper
• siRNA target site secondary structure predictions using local stable substructures

  2005cited by this paper
• mRNA translation is not a prerequisite for small interfering RNA-mediated mRNA cleavage.

  2005influential reference
• Purified Argonaute2 and an siRNA form recombinant human RISC

  2005influential reference
• Target accessibility dictates the potency of human RISC

  2005cited by this paper
• Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage.

  2005cited by this paper
• Local RNA target structure influences siRNA efficacy: systematic analysis of intentionally designed binding regions.

  2005cited by this paper
• Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes.

  2005cited by this paper
• RNAi: the nuts and bolts of the RISC machine.

  2005influential reference
• Mechanistic insights aid computational short interfering RNA design.

  2005cited by this paper
• Argonaute2 cleaves the anti-guide strand of siRNA during RISC activation.

  2005cited by this paper
• Principles of MicroRNA–Target Recognition

  2005cited by this paper
• Specificity of microRNA target selection in translational repression.

  2004cited by this paper
• RISC is a 5' phosphomonoester-producing RNA endonuclease.

  2004influential reference
• Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

  2004influential reference
• Kinetic analysis of the RNAi enzyme complex

  2004influential reference
• Sequence-Specific Inhibition of Small RNA Function

  2004influential reference
• The gene-silencing efficiency of siRNA is strongly dependent on the local structure of mRNA at the targeted region.

  2004cited by this paper
• The RNA-induced silencing complex is a Mg2+-dependent endonuclease.

  2004influential reference
• Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis.

  2004cited by this paper
• A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila.

  2004influential reference
• Argonaute2 Is the Catalytic Engine of Mammalian RNAi

  2004influential reference
• Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity

  2004influential reference
• Asymmetry in the assembly of the RNAi enzyme complex.

  2003cited by this paper
• Functional siRNAs and miRNAs exhibit strand bias.

  2003cited by this paper
• A micrococcal nuclease homologue in RNAi effector complexes

  2003cited by this paper
• The nuclear RNase III Drosha initiates microRNA processing

  2003cited by this paper
• Asymmetry in the Assembly of the RNAi Enzyme Complex

  2003cited by this paper
• Mfold web server for nucleic acid folding and hybridization prediction

  2003cited by this paper
• Identification of eight members of the Argonaute family in the human genome.

  2003cited by this paper
• A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins.

  2002cited by this paper
• Fragile X-related protein and VIG associate with the RNA interference machinery.

  2002cited by this paper
• Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.

  2002influential reference
• Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction.

  2002cited by this paper
• Small RNAs in bacteria: diverse regulators of gene expression in response to environmental changes.

  2002cited by this paper
• The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs.

  2002cited by this paper
• Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate

  2001influential reference
• ATP requirements and small interfering RNA structure in the RNA interference pathway.

  2001cited by this paper
• Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

  2001influential reference
• RNA interference is mediated by 21- and 22-nucleotide RNAs.

  2001influential reference
• Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi

  2001influential reference
• A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans

  2001cited by this paper
• Role for a bidentate ribonuclease in the initiation step of RNA interference

  2001cited by this paper
• RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.

  2000influential reference
• An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

  2000cited by this paper
• Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.

  1999cited by this paper
• Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

  1998cited by this paper
• Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.

  1990cited by this paper
• References and Notes Materials and Methods Som Text Fig. S1 Table S1 References a Microrna in a Multiple- Turnover Rnai Enzyme Complex

  year unknowncited by this paper
• Role for a bidentate ribonuclease in the initiation step of RNA interference

  year unknowncited by this paper

• Divergent Fates of Kidney-Resident Polyomaviruses: Stable Shedding Versus Near-Silent Persistence

  2026cites this paper
• Identification of miRNAs Expression Characteristics and Biomarkers in Serum‐Derived Exosomes of Wilson’s Disease Patients

  2026cites this paper
• Mechanistic intracellular PK/PD modeling to inform development strategies for small interfering RNA therapeutics

  2025cites this paper
• SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability

  2025cites this paper
• Mapping effective microRNA pairing beyond the seed using abasic modifications

  2025cites this paper
• miRNA-target complementarity in cnidarians resembles its counterpart in plants

  2025cites this paper
• Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity

  2025cites this paper
• Mechanistic insights into RNA cleavage by human Argonaute2–siRNA complex

  2025cites this paper
• Antiviral nanomedicine: Advantages, mechanisms and advanced therapies

  2025cites this paper
• A new level of RNA-based plant protection: dsRNAs designed from functionally characterized siRNAs highly effective against Cucumber mosaic virus

  2025cites this paper
• Nanotechnological approaches in topical RNAi therapy for skin diseases

  2025cites this paper
• Phosphorothioate and 2'-Fluoro Ribose Substitution-Mediated Hydrophobic Effect Empowers RNA Probes for Highly Sensitive and Subtype-Selective miRNA Imaging.

  2025cites this paper
• Functional microRNA targeting without seed pairing

  2025cites this paper
• Splice-switching ASOs targeting the AURKA 5' UTR collapse an SRSF1-AURKA-MYC oncogenic circuit in pancreatic cancer.

  2025cites this paper
• Adipose-Derived Exosomes: mediators of crosstalk between Adipose tissue and cancer

  2025cites this paper
• Splice-switching ASOs targeting an Alu-derived exon in the AURKA 5’UTR collapse an SRSF1-AURKA-MYC oncogenic circuit in pancreatic cancer

  2025cites this paper
• From Bits to Bonds: High-Throughput Virtual Screening of Ribonucleic Acid Nanocarriers Using a Combinatorial Approach of Machine Learning and Molecular Dynamics

  2025cites this paper
• Development of siRNA therapeutics to combat microbial infections: a bibliometric analysis

  2025cites this paper
• TarP: A microRNA target gene prediction tool utilizing a polymorphic structured alignment approach.

  2025cites this paper
• Biomineralized Zn2+-siRNA complex enhances CD47 blockade therapy by facilitating phagocytosis and mtDNA sensing

  2025cites this paper
• IGF2BP3 remodels the microRNA targeting landscape in MLL-AF4 leukemia

  2025cites this paper
• A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals

  2025cites this paper
• A bioinformatic ecological risk assessment framework for externally applied dsRNA-based biopesticides.

  2025cites this paper
• Nanoparticles and Nanocarriers for Managing Plant Viral Diseases

  2025cites this paper
• The expanding role of microRNAs in the biology and control of veterinary parasitic nematodes

  2025cites this paper
• Recent Update on siRNA Therapeutics

  2025cites this paper
• Sequence, structure, and affinity of miR-34a binding sites determine repression efficacy

  2025cites this paper
• A bioinformatics framework for human health risk assessment of externally applied dsRNA-based biopesticides

  2024cites this paper
• The guide RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex

  2024cites this paper
• Nanotechnology strategies to address challenges in topical and cellular delivery of siRNAs in skin disease therapy.

  2024cites this paper
• The Role of Exosomes in Central Immune Tolerance and Myasthenia Gravis

  2024cites this paper
• Short 2′-O-methyl/LNA oligomers as highly-selective inhibitors of miRNA production in vitro and in vivo

  2024cites this paper
• β-Carotene protects against α-amanitin nephrotoxicity via modulation of oxidative, autophagic, nitric oxide signaling, and polyol pathways in rat kidneys.

  2024cites this paper
• Glioblastoma therapy: State of the field and future prospects.

  2024cites this paper
• Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs

  2024cites this paper
• A new level of RNA-based plant protection - dsRNAs designed from functionally characterized siRNAs highly effective against Cucumber Mosaic Virus

  2024cites this paper
• Dysregulated MicroRNAs and long non-coding RNAs associated with extracellular matrix stiffness.

  2024cites this paper
• Recent Progress of Small Interfering RNA Delivery on the Market and Clinical Stage.

  2024cites this paper
• The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.

  2024cites this paper
• Current status of nucleic acid therapy and its new progress in cancer treatment.

  2024cites this paper
• Unraveling the complex roles of macrophages in obese adipose tissue: an overview

  2024cites this paper
• Therapeutic synthetic and natural materials for immunoengineering.

  2024cites this paper
• Advanced computational predictive models of miRNA-mRNA interaction efficiency.

  2024cites this paper
• Evaluation of the effect of RNA secondary structure on Cas13d-mediated target RNA cleavage

  2024cites this paper
• Preferential cleavage of upstream targets in concatenated miRNA/siRNA target sites support a 5'-3' scanning model for RISC target recognition.

  2024cites this paper
• Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1

  2023cites this paper
• Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq.

  2023cites this paper
• Engineering metabolism to modulate immunity.

  2023cites this paper
• When Argonaute takes out the ribonuclease sword

  2023cites this paper
• Discovery of the major 15–30 nt mammalian small RNAs, their biogenesis and function

  2023cites this paper
• Functionally comparable but evolutionarily distinct nucleotide-targeting effectors help identify conserved paradigms across diverse immune systems.

  2023cites this paper
• miRNAs orchestration of cardiovascular diseases - Particular emphasis on diagnosis, and progression.

  2023cites this paper
• The potential role of miRNAs in the pathogenesis of cardiovascular diseases - A focus on signaling pathways interplay.

  2023cites this paper
• Injectable Hydrogel Containing RNA-Loaded Liposomes Alleviates Chondrocyte Inflammation by Disrupting BAK1-Mediated mtDNA Maintenance

  2023cites this paper
• Follicular fluid exosomes inhibit BDNF expression and promote the secretion of chemokines in granulosa cells by delivering miR-10b-5p.

  2023cites this paper
• Short 2′-O-methyl/LNA oligomers as highly-selective inhibitors of miRNA production in vitro and in vivo

  2023cites this paper
• Asymmetric trichotomous partitioning overcomes dataset limitations in building machine learning models for predicting siRNA efficacy

  2023cites this paper
• The long-noncoding RNA PILNCR2 increases low phosphate tolerance in maize by interfering with miRNA399-guided cleavage of ZmPHT1s.

  2023cites this paper
• Insights in piRNA targeting rules

  2023cites this paper
• Critical points for the design and application of RNA silencing constructs for plant virus resistance.

  2023cites this paper
• Evitar: designing anti-viral RNA therapies against future RNA viruses

  2022cites this paper
• High-throughput biochemical profiling reveals functional adaptation of a bacterial Argonaute.

  2022cites this paper
• Probing in vivo RNA Structure With Optimized DMS-MaPseq in Rice

  2022cites this paper
• A tweak and a peek: How Cas9 pries open double-stranded DNA to check its sequence

  2022cites this paper
• MicroRNA 3′-compensatory pairing occurs through two binding modes, with affinity shaped by nucleotide identity and position

  2022cites this paper
• A Structural View of miRNA Biogenesis and Function

  2022cites this paper
• Prediction of putative potential siRNAs for inhibiting SARS-CoV-2 strains, including variants of concern and interest

  2022cites this paper
• Allele-specific silencing by RNAi of R92Q and R173W mutations in cardiac troponin T

  2022cites this paper
• SiRNAs with Neutral Phosphate Triester Hydrocarbon Tails Exhibit Carrier-Free Gene-Silencing Activity.

  2022cites this paper
• The Extensive Regulation of MicroRNA in Immune Thrombocytopenia

  2022cites this paper
• Understanding the Binding Affinity and Specificity of miRNAs: A Molecular Dynamics Study

  2022cites this paper
• Distinct Response of Circulating microRNAs to the Treatment of Pancreatic Cancer Xenografts with FGFR and ALK Kinase Inhibitors

  2022cites this paper
• Prokaryotic Argonaute Proteins as a Tool for Biotechnology

  2022cites this paper
• Oxidative Stress-Mediated miRNA Regulation in Cancer

  2022cites this paper
• Transcriptomics and RNA-Based Therapeutics as Potential Approaches to Manage SARS-CoV-2 Infection

  2022cites this paper
• CRISPR guides induce gene silencing in plants in the absence of Cas

  2022cites this paper
• Nucleus‐specific RNAi nanoplatform for targeted regulation of nuclear lncRNA function and effective cancer therapy

  2022cites this paper
• HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution

  2022cites this paper
• New Insights on the Integrated Management of Plant Diseases by RNA Strategies: Mycoviruses and RNA Interference

  2022cites this paper
• Structural basis for RNA slicing by a plant Argonaute

  2022cites this paper
• Polymeric micelles and cancer therapy: an ingenious multimodal tumor-targeted drug delivery system

  2022cites this paper
• Anatomy of four human Argonaute proteins

  2022cites this paper
• CRISPR Editing Enables Consequential Tag-Activated MicroRNA-Mediated Endogene Deactivation

  2022cites this paper
• Asymmetric trichotomous data partitioning enables development of predictive machine learning models using limited siRNA efficacy datasets

  2022cites this paper
• Emerging Gene Manipulation Strategies for the Treatment of Monogenic Eye Disease

  2022cites this paper
• Nanomedicine based approaches for combating viral infections.

  2021cites this paper
• Alterations in circulating extracellular vesicles underlie social stress‐induced behaviors in mice

  2021cites this paper
• GPC5 suppresses lung cancer progression and metastasis via intracellular CTDSP1/AhR/ARNT signaling axis and extracellular exosome secretion

  2021cites this paper
• Exosomal miR-224 contributes to hemolymph microbiota homeostasis during bacterial infection in crustacean.

  2021cites this paper
• scanMiR: a biochemically-based toolkit for versatile and efficient microRNA target prediction

  2021cites this paper
• RNA silencing by CRISPR in plants does not require Cas13

  2021cites this paper
• Long non-coding RNAs HERH-1 and HERH-4 facilitate cyclin A2 expression and accelerate cell cycle progression in advanced hepatocellular carcinoma

  2021cites this paper
• Employing siRNA tool and its delivery platforms in suppressing cisplatin resistance: Approaching to a new era of cancer chemotherapy.

  2021cites this paper
• Gene Reporter Assays to Study miRNA Function.

  2021cites this paper
• Increased BMSC exosomal miR-140-3p alleviates bone degradation and promotes bone restoration by targeting Plxnb1 in diabetic rats

  2021cites this paper
• Emergent RNA-RNA interactions can promote stability in a nascent phototrophic endosymbiosis

  2021cites this paper
• Pre-Clinical and Clinical Applications of Small Interfering RNAs (siRNA) and Co-Delivery Systems for Pancreatic Cancer Therapy

  2021cites this paper
• Exosomes-loaded thermosensitive hydrogels for corneal epithelium and stroma regeneration.

  2021cites this paper
• Platelet microRNAs inhibit primary tumor growth via broad modulation of tumor cell mRNA expression in ectopic pancreatic cancer in mice

  2021cites this paper
• Cancer Nanotechnology for Drug Targeting and Delivery Approaches

  2021cites this paper