A β4 Isoform-specific Interaction Site in the Carboxyl-terminal Region of the Voltage-dependent Ca2+ Channel α1A Subunit*

The voltage-gated calcium channel β subunit is a cytoplasmic protein that stimulates activity of the channel-forming subunit, α1, in several ways. Complementary binding sites on α1 and β have been identified that are highly conserved among isoforms of the two subunits, but this interaction alone does not account for all of the functional effects of the β subunit. We describe here the characterization in vitro of a second interaction, involving the carboxyl-terminal cytoplasmic domain of α1A and showing specificity for the β4 (and to a lesser extent β2a) isoform. A deletion and chimera approach showed that the carboxyl-terminal region of β4, poorly conserved between β isoforms, contains the interaction site and plays a role in the regulation of channel inactivation kinetics. This is the first demonstration of a molecular basis for the specificity of functional effects seen for different combinations of these two channel components.

• Publication year

  1998

• Venue

  Journal of Biological Chemistry

• Publication date

  1998-01-23

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.273.4.2361PMID 9442082
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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