Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells.

The monokine interleukin-1 (IL-1) inhibits endothelial cell growth and induces prostacyclin production in human endothelial cells. Since cyclooxygenase (Cox) is the rate-limiting enzyme in the synthesis of prostanoids, we evaluated the ability of IL-1 to stimulate Cox expression by human umbilical vein endothelial cells (HUVEC) in vitro. Our data demonstrate that 1) the Cox mRNA is expressed at low levels in untreated cells; 2) IL-1 alpha induces the Cox mRNA within 2 h, and this induction is sustained for more than 24 h; 3) IL-1 alpha induction is dose-dependent; 4) cycloheximide potentiates the induction of the Cox mRNA by IL-1 alpha while actinomycin D prevents the induction, and 5) IL-1 alpha also stimulates Cox production in a time-dependent fashion which correlates with the increase in prostacyclin synthesis. These data suggest that Cox is an immediate-early gene induced by IL-1 in HUVEC and may contribute to the regulation of the endothelial cell differentiation pathway in vitro.

• Publication year

  1990

• Venue

  Journal of Biological Chemistry

• Publication date

  1990-07-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(19)38515-1PMID 1694171
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme.

  1989cited by this paper
• Differential recovery of prostacyclin synthesis in cultured vascular endothelial vs. smooth muscle cells after inactivation of cyclooxygenase with aspirin.

  1989cited by this paper
• Temporal and pharmacological division of fibroblast cyclooxygenase expression into transcriptional and translational phases.

  1989cited by this paper
• Regulation of prostaglandin H synthase mRNA levels and prostaglandin biosynthesis by platelet-derived growth factor.

  1989cited by this paper
• Epidermal growth factor stimulation of prostaglandin E2 biosynthesis in amnion cells. Induction of prostaglandin H2 synthase.

  1988cited by this paper
• Stimulation of de novo synthesis of prostaglandin G/H synthase in human endothelial cells by phorbol ester.

  1988cited by this paper
• Regulation of fibroblast cyclooxygenase synthesis by interleukin-1.

  1988cited by this paper
• Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism. Induction of de novo synthesis of prostaglandin H synthase.

  1988cited by this paper
• Biology of interleukin 1

  1988cited by this paper
• Transcriptional and posttranscriptional control of c-fos gene expression in human monocytes

  1988cited by this paper
• Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

  1988cited by this paper
• Additive effects of IL-1 and TNF on induction of prostacyclin synthesis in human vascular endothelial cells.

  1988cited by this paper
• Induction of proto-oncogene JUN/AP-1 by serum and TPA

  1988cited by this paper
• Inhibition of endothelial cell proliferation by gamma-interferon

  1987cited by this paper
• Inhibitory effect of human recombinant interleukin-1 alpha and beta on growth of human vascular endothelial cells.

  1987cited by this paper
• Modulation of endothelial cell functions by different molecular species of interleukin 1.

  1987cited by this paper
• Regulation of the fibrinolytic system of cultured human vascular endothelium by interleukin 1.

  1986cited by this paper
• Stimulation of prostaglandin E2 synthesis in cloned osteoblastic cells of mouse (MC3T3-E1) by epidermal growth factor.

  1986cited by this paper
• Isolation of the cDNA for human prostaglandin H synthase.

  1986cited by this paper
• Interleukin 1 and lipopolysaccharide induce an inhibitor of tissue-type plasminogen activator in vivo and in cultured endothelial cells

  1986cited by this paper
• Inhibitors of protein and RNA synthesis cause a rapid block in prostaglandin production at the prostaglandin synthase step.

  1986cited by this paper
• Interleukin 1 induces endothelial cell synthesis of plasminogen activator inhibitor

  1986cited by this paper
• Arachidonic acid metabolism.

  1986cited by this paper
• Effect of protein synthesis inhibitors on growth factor activation of c-fos, c-myc, and actin gene transcription

  1986cited by this paper
• Interleukin 1 induces endothelial cell procoagulant while suppressing cell-surface anticoagulant activity.

  1986cited by this paper
• A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.

  1986cited by this paper
• Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.

  1985cited by this paper
• Human endothelial cell cultures: Phenotypic modulation by leukocyte interleukins

  1985cited by this paper
• Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion.

  1985cited by this paper
• Restoration of prostacyclin synthase in vascular smooth muscle cells after aspirin treatment: regulation by epidermal growth factor.

  1985cited by this paper
• Human platelet-derived growth factor stimulates prostaglandin synthesis by activation and by rapid de novo synthesis of cyclooxygenase.

  1985cited by this paper
• Prostacyclin synthesis induced in vascular cells by interleukin-1.

  1985cited by this paper
• Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein

  1984cited by this paper
• Chromosomal location of the co-expressed human skeletal and cardiac actin genes.

  1984cited by this paper
• Genomic sequencing.

  1984cited by this paper
• Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene

  1984cited by this paper
• Induction of c-fos gene and protein by growth factors precedes activation of c-myc

  1984cited by this paper
• Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells

  1984cited by this paper
• Eicosanoids: Prostaglandins, Thromboxanes, Leukotrienes, and Other Derivatives of Carbon‐20 Unsaturated Fatty Acids

  1982cited by this paper
• Organizational behavior of human umbilical vein endothelial cells

  1982cited by this paper
• Serial propagation of human endothelial cells in vitro

  1981cited by this paper
• Evidence for a peroxide-initiated free radical mechanism of prostaglandin biosynthesis.

  1980cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glands.

  1977cited by this paper
• Human arterial and venous tissues generate prostacyclin (prostaglandin x), a potent inhibitor of platelet aggregation.

  1977cited by this paper
• Acetylation of prostaglandin synthase by aspirin.

  1975cited by this paper
• Oxygenation of polyunsaturated fatty acids during prostaglandin biosynthesis by sheep vesicular gland.

  1972cited by this paper
• The endothelial cell.

  1966cited by this paper

• Gut microbiome modulation by Weifuchun Capsules alleviates chronic atrophic gastritis: a combined microbiota and metabolomics approach

  2025cites this paper
• Screening of antimicrobial and antiinflammatory activities of three lichenized fungal extracts collected from Northwest Anatolia (Türkiye)

  2024cites this paper
• The Physiology of Enteric Glia

  2024cites this paper
• Binding Modes of the Metabolites Docosahexaenoic Acid, Eicosapentaenoic acid, and Eicosapentaenoic Acid Ethyl Ester from Caulerpa racemosa as COX-2 Inhibitors Revealed via Metabolomics and Molecular Dynamics

  2024cites this paper
• Development of Novel 1,3-Disubstituted-2-Thiohydantoin Analogues with Potent Anti-Inflammatory Activity; In Vitro and In Silico Assessments

  2022cites this paper
• Extracellular Histones Activate Endothelial NLRP3 Inflammasome and are Associated with a Severe Sepsis Phenotype

  2022cites this paper
• A Systematic Review of the Cyclooxygenase-2 (COX-2) Expression in Rectal Cancer Patients Treated with Preoperative Radiotherapy or Radiochemotherapy

  2021cites this paper
• Acute P38-Mediated Enhancement of P2X3 Receptor Currents by TNF-α in Rat Dorsal Root Ganglion Neurons

  2021cites this paper
• Bone Cells Differentiation: How CFTR Mutations May Rule the Game of Stem Cells Commitment?

  2021cites this paper
• TNF-α acutely enhances acid-sensing ion channel currents in rat dorsal root ganglion neurons via a p38 MAPK pathway

  2021cites this paper
• Neuro-Immunity and Gut Dysbiosis Drive Parkinson’s Disease-Induced Pain

  2021cites this paper
• Chronic prostatitis/chronic pelvic pain syndrome increases susceptibility to seizures in rats and alters brain levels of IL-1β and IL-6.

  2019cites this paper
• The anti-gastritis activity of an exopolysaccharide from Rhizopus nigricans

  2019cites this paper
• Transcriptomics in pain research: insights from new and old technologies.

  2018cites this paper
• The role of cyclooxygenase-2 in the protection against apoptosis in vascular endothelial cells induced by cigarette smoking.

  2017cites this paper
• Chapter 2 Mouse Models of Intestinal Cancer

  2017cites this paper
• A novel in vitro macrophage senescence model to study macrophaging

  2017cites this paper
• Upregulation of Cyclooxygenase-2/Prostaglandin E2 (COX-2/PGE2) Pathway Member Multiple Drug Resistance-Associated Protein 4 (MRP4) and Downregulation of Prostaglandin Transporter (PGT) and 15-Prostaglandin Dehydrogenase (15-PGDH) in Triple-Negative Breast Cancer

  2016cites this paper
• MAPK-dependent pathway -ENaC mRNA in collecting duct cells by p38 α Cycloheximide increases glucocorticoid-stimulated

  2016cites this paper
• THE EFFECTS OF EXOGENOUS CYCLOOXYGENASE ACTIVITY AND CULTURED ENDOTHELIAL CELLS 11 ARACHIDONIC ACID ON ISOFORMS EXPRESSED IN

  2015cites this paper
• The role of slow and persistent TTX-resistant sodium currents in acute tumor necrosis factor-α-mediated increase in nociceptors excitability.

  2015cites this paper
• Study of the expression and regulation of the autocrine loop components of the cyclooxygenase pathway and their implication in aspirin exacerbated respiratory disease

  2015cites this paper
• Antiinflammatory and antioxidant effects of H2O2 generated by natural sources in Il1β-treated human endothelial cells.

  2015cites this paper
• THE INDUCIBLE ISOFORM OF CYCLOOXYGENASE ( COX-2 ) : NEW TARGET FOR ANTIINFLAMMA TORY THERAPY

  2015cites this paper
• NFATc1 activation promotes the invasion of U251 human glioblastoma multiforme cells through COX-2.

  2015cites this paper
• Increased excitability of medium‐sized dorsal root ganglion neurons by prolonged interleukin‐1β exposure is K+ channel dependent and reversible

  2015cites this paper
• COX2- Inhibitors and Their Role in Cancer Prevention and Treatment

  2015cites this paper
• Inflammation and Lung Cancer: The Link to Angiogenesis

  2015cites this paper
• Repurposing of Metformin and Aspirin by Targeting AMPK-mTOR and Inflammation for Pancreatic Cancer Prevention and Treatment

  2014cites this paper
• Types of fish consumed and fish preparation methods in relation to pancreatic cancer incidence: the VITAL Cohort Study.

  2013cites this paper
• Tissue Injury and Related Mediators of Pain Exacerbation

  2013cites this paper
• Is Helicobacter pylori the infectious trigger for headache?: A review.

  2013cites this paper
• Management of Hyperuricemia and Gout

  2013cites this paper
• Isoprostane, Phytoprostane, Nitroölsäuren und Eiöl als Modulatoren der Genexpression : Zusammenhang zwischen oxidativem Stress und Atherosklerose

  2013cites this paper
• Cryptorchidism-induced CFTR down-regulation results in disruption of testicular tight junctions through up-regulation of NF-κB/COX-2/PGE2.

  2012cites this paper
• Non-steroidal and steroidal anti-inflammatory use in the context of orthodontic movement.

  2012cites this paper
• Effects of estrogen on endothelial prostanoid production and cyclooxygenase-2 and heme oxygenase-1 expression.

  2012cites this paper
• CFTR negatively regulates cyclooxygenase‐2‐PGE2 positive feedback loop in inflammation

  2012cites this paper
• Inhibition of Nitric Oxide Production, iNOS and COX-2 Expression of Ergosterol Derivatives from Phellinus pini

  2012influential citation
• d-Limonene modulates inflammation, oxidative stress and Ras-ERK pathway to inhibit murine skin tumorigenesis

  2012cites this paper
• INSTITUTO POLITÉCNICO NACIONAL ESCUELA SUPERIOR DE MEDICINA SECCIÓN DE ESTUDIOS DE POSGRADO E INVESTIGACIÓN

  2011cites this paper
• Mechanisms of nociceptive transduction and transmission: a machinery for pain sensation and tools for selective analgesia.

  2011cites this paper
• Methyl deficient diet aggravates experimental colitis in rats

  2011cites this paper
• Follicular Dendritic Cells, Resting CD4+ T Cells and Human Immunodeficiency Virus Expression

  2011cites this paper
• Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells

  2011cites this paper
• Metabolomics‐Lipidomics of Eicosanoids and Docosanoids Generated by Phagocytes

  2011cites this paper
• Inhibitory effect of astragalin on expression of lipopolysaccharide-induced inflammatory mediators through NF-κB in macrophages

  2011cites this paper
• Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-kappaB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages.

  2010cites this paper
• Regulating effects of herb cake-partitioned moxibustion on the expression of p53 and C-myc protein in rats with ulcerative colitis

  2010cites this paper
• Aspirin Induced Asthma- a Review

  2010cites this paper
• Regulation of survival, proliferation, invasion, angiogenesis, and metastasis of tumor cells through modulation of inflammatory pathways by nutraceuticals

  2010cites this paper
• Apoptosis signal‐regulating kinase 1 in peptidoglycan‐induced COX‐2 expression in macrophages

  2010cites this paper
• Sesquiterpene lactone fraction from Artemisia khorassanica inhibits inducible nitric oxide synthase and cyclooxygenase-2 expression through the inactivation of NF-κB

  2010cites this paper
• The role of translational regulation in ultraviolet light‐induced cyclooxygenase‐2 expression

  2009cites this paper
• Peroxynitrite as regulator of vascular prostanoid synthesis.

  2009cites this paper
• Transglutaminase-dependent antiproliferative and differentiative properties of nimesulide on B16-F10 mouse melanoma cells

  2009cites this paper
• Peptidoglycan Induces Cyclooxygenase-2 Expression in Macrophages by Activating the Neutral Sphingomyelinase-Ceramide Pathway*

  2009cites this paper
• Mouse Models of Intestinal Cancer.

  2009cites this paper
• Influence des donneurs de méthyle et du métabolisme de l'homocystéine dans la physiopatholie des MICI : études de population et modèle expérimental chez le raton carencé

  2009cites this paper
• Relation between Cyclooxygenase-2 and Polo-like Kinase-1 in Non-Small Cell Lung Cancer

  2009cites this paper
• Epithelial Cell Gene Expression Induced by Intracellular Staphylococcus aureus

  2009cites this paper
• Voltage-gated sodium channels in pain states: role in pathophysiology and targets for treatment.

  2009cites this paper
• Does COX2-dependent PGE2 play a role in neuropathic pain?

  2008cites this paper
• Vein wall re-endothelialization after deep vein thrombosis is improved with low-molecular-weight heparin.

  2008cites this paper
• Early stress response of human nasal respiratory epithelia after exposure to 1-methoxypropanol-2.

  2008cites this paper
• The effects of isoimperatorin isolated from Agelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

  2008cites this paper
• Endothelial Cells in Allograft Rejection

  2008cites this paper
• Nociceptors Are Interleukin-1β Sensors

  2008influential citation
• The presence of the mu-opioid receptor in the isthmus of mare oviduct.

  2008cites this paper
• Role of Prostaglandin Synthesis and Cyclooxygenase-2 in Prostate Cancer and Metastasis

  2008cites this paper
• Meso-dihydroguaiaretic acid isolated from Saururus chinensis inhibits cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

  2008cites this paper
• Chemoprevention of Scutellaria bardata on human cancer cells and tumorigenesis in skin cancer

  2007cites this paper
• Metastasis of prostate cancer

  2007cites this paper
• ["CLINICAL AND ANIMAL STUDIES OF LIPID-DERIVED PROTEIN MODIFICATIONS IN AUTISM, KIDNEY DIALYSIS, KERATITIS AND AGE-RELATED MACULAR DEGENERATION"]

  2007cites this paper
• Excitable membranes, lipid messengers, and immediate-early genes

  2007cites this paper
• Anti-inflammatory effects of hydroxycinnamic acid derivatives.

  2007cites this paper
• Short Communication Disruption of the CD40-CD40 Ligand System Prevents an Oxygen-Induced Respiratory Distress Syndrome

  2007cites this paper
• Prostanoid Receptors in the Human Vascular Wall

  2007cites this paper
• Biochemical-molecular markers in unilateral ureteral obstruction.

  2007cites this paper
• Ultrasound induces cyclooxygenase-2 expression through integrin, integrin-linked kinase, Akt, NF-kappaB and p300 pathway in human chondrocytes.

  2007cites this paper
• ROLE OF HUMAN CYCLOOXYGENASE-2 IN THE BIOACTIVATION OF DAPSONE AND SULFAMETHOXAZOLE

  2006cites this paper
• Vitamin A active metabolite, all‐trans retinoic acid, induces spinal cord sensitization. II. Effects after intrathecal administration

  2006cites this paper
• Synthesis, molecular modelling and enzymatic evaluation of (+/-)3,5-diphenyl-2-thioxoimidazolidin-4-ones as new potential cyclooxygenase inhibitors.

  2006cites this paper
• Open House: The Ethics of Studying Children at Home

  2006cites this paper
• Elevated expression of cyclooxygenase-2 is a negative prognostic factor for overall survival in intrahepatic cholangiocarcinoma

  2006cites this paper
• Regulation of nicotine-induced cyclooxygenase-2 protein expression in human gingival fibroblasts

  2006cites this paper
• NF-kappaB, inflammation and pancreatic carcinogenesis: NF-kappaB as a chemoprevention target (review).

  2006cites this paper
• Infiltration of inflammatory cells plays an important role in matrix metalloproteinase expression and activation in the heart during sepsis.

  2006cites this paper
• The Gastrointestinal Safety and Effect on Disease Activity of Etoricoxib, a Selective Cox-2 Inhibitor in Inflammatory Bowel Diseases

  2006cites this paper
• Gastric mucosal cell model for estimating relative gastrointestinal toxicity of non-steroidal anti-inflammatory drugs.

  2006cites this paper
• The enigma of prostatitis. Some historical perspectives on the pathogenesis of prostatitis

  2006cites this paper
• Targeting invading macrophage- derived PGE2, IL-6 and calcitonin gene-related peptide in injured nerve to treat neuropathic pain

  2006cites this paper
• The Basic Science of Wound Healing

  2006cites this paper
• Effect of pro-inflammatory cytokines on the toxicity of the arylhydroxylamine metabolites of sulphamethoxazole and dapsone in normal human keratinocytes.

  2006cites this paper
• Naturally occurring biflavonoid, ochanflavone, inhibits cyclo-oxygenases-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

  2006cites this paper
• Peroxisome-proliferator-activated receptor alpha agonists inhibit cyclo-oxygenase 2 and vascular endothelial growth factor transcriptional activation in human colorectal carcinoma cells via inhibition of activator protein-1.

  2006cites this paper
• Reciprocal regulation of cyclooxygenase-2 and 15-hydroxyprostaglandin dehydrogenase expression in A549 human lung adenocarcinoma cells.

  2006cites this paper
• Vitamin A active metabolite, all‐trans retinoic acid, induces spinal cord sensitization. I. Effects after oral administration

  2006influential citation
• Up-regulation of cyclooxygenase-2 by interleukin-1beta in colon carcinoma cells.

  2006cites this paper
• Sulfonamide-induced cutaneous drug reactions: Role of bioactivation, oxidative stress and folate deficiency

  2006cites this paper