An improved purification procedure for the soluble [NiFe]-hydrogenase of Ralstonia eutropha: new insights into its (in)stability and spectroscopic properties

No abstract is available for this paper.

• Publication year

  2006

• Venue

  JBIC Journal of Biological Inorganic Chemistry

• Publication date

  2006-01-18

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1007/s00775-005-0075-4PMID 16418856
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structural and oxidation-state changes at its nonstandard Ni-Fe site during activation of the NAD-reducing hydrogenase from Ralstonia eutropha detected by X-ray absorption, EPR, and FTIR spectroscopy.

  2005cited by this paper
• Structural differences between the ready and unready oxidized states of [NiFe] hydrogenases

  2005cited by this paper
• The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH

  2005cited by this paper
• The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide.

  2005cited by this paper
• Structural differences between the ready and unready oxidized states of [NiFe] hydrogenases

  2005cited by this paper
• The Auxiliary Protein HypX Provides Oxygen Tolerance to the Soluble [NiFe]-Hydrogenase of Ralstonia eutropha H16 by Way of a Cyanide Ligand to Nickel*

  2004cited by this paper
• An orientation-selected ENDOR and HYSCORE study of the Ni-C active state of Desulfovibrio vulgaris Miyazaki F hydrogenase

  2004cited by this paper
• Hydrogen-induced activation of the [NiFe]-hydrogenase from Allochromatium vinosum as studied by stopped-flow infrared spectroscopy.

  2004cited by this paper
• Reactions of H2, CO, and O2 with active [NiFe]-hydrogenase from Allochromatium vinosum. A stopped-flow infrared study.

  2004cited by this paper
• The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen

  2004cited by this paper
• Selective release and function of one of the two FMN groups in the cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha.

  2004cited by this paper
• Relativistic DFT calculation of the reaction cycle intermediates of [NiFe] hydrogenase: a contribution to understanding the enzymatic mechanism.

  2004cited by this paper
• Electrochemical potential-step investigations of the aerobic interconversions of [NiFe]-hydrogenase from Allochromatium vinosum: insights into the puzzling difference between unready and ready oxidized inactive states.

  2004cited by this paper
• The activation of the [NiFe]-hydrogenase from Allochromatium vinosum. An infrared spectro-electrochemical study

  2004cited by this paper
• Quantitative amino acid analysis of bovine NADH:ubiquinone oxidoreductase (Complex I) and related enzymes. Consequences for the number of prosthetic groups.

  2003cited by this paper
• Single crystal EPR studies of the reduced active site of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F.

  2003cited by this paper
• Direct detection of a hydrogen ligand in the [NiFe] center of the regulatory H2-sensing hydrogenase from Ralstonia eutropha in its reduced state by HYSCORE and ENDOR spectroscopy.

  2003cited by this paper
• UvA-DARE ( Digital Academic Repository ) Nickel hydrogenases : in search for the active site

  2002cited by this paper
• Structural, redox, and mechanistic parameters for cysteine-sulfenic acid function in catalysis and regulation.

  2001cited by this paper
• Relativistic DFT calculations of the paramagnetic intermediates of [NiFe] hydrogenase. Implications for the enzymatic mechanism.

  2001cited by this paper
• The [NiFe] hydrogenase from Allochromatiumvinosum studied in EPR-detectable states: H/D exchange experiments that yield new information about the structure of the active site

  2001cited by this paper
• Hydrogen as a Fuel : Learning from Nature

  2001cited by this paper
• Pre-steady-state kinetics of the reactions of [NiFe]-hydrogenase from Chromatium vinosum with H2 and CO.

  2001cited by this paper
• Structural examination of the nickel site in chromatium vinosum hydrogenase: redox state oscillations and structural changes accompanying reductive activation and CO binding.

  2000cited by this paper
• Unusual FTIR and EPR properties of the H2‐activating site of the cytoplasmic NAD‐reducing hydrogenase from Ralstonia eutropha

  2000cited by this paper
• Learning from hydrogenases: location of a proton pump and of a second FMN in bovine NADH–ubiquinone oxidoreductase (Complex I)

  2000cited by this paper
• Carbon Monoxide and Cyanide as Intrinsic Ligands to Iron in the Active Site of [NiFe]-Hydrogenases

  1999cited by this paper
• The crystal structure of a reduced [NiFeSe] hydrogenase provides an image of the activated catalytic center.

  1999cited by this paper
• Removal of the bridging ligand atom at the Ni-Fe active site of [NiFe] hydrogenase upon reduction with H2, as revealed by X-ray structure analysis at 1.4 A resolution.

  1999cited by this paper
• Protein-sulfenic acids: diverse roles for an unlikely player in enzyme catalysis and redox regulation.

  1999cited by this paper
• Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.

  1999cited by this paper
• Transcriptional Regulation of Alcaligenes eutrophus Hydrogenase Genes

  1998cited by this paper
• Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms

  1998cited by this paper
• Modulation of the redox potentials of FMN in Desulfovibrio vulgaris flavodoxin: thermodynamic properties and crystal structures of glycine-61 mutants.

  1998cited by this paper
• Subforms and In Vitro Reconstitution of the NAD-Reducing Hydrogenase of Alcaligenes eutrophus

  1998cited by this paper
• NADH‐Induced Changes of the Nickel Coordination within the Active Site of the Soluble Hydrogenase from Alcaligenes eutrophus: XAFS Investigations on Three States Distinguishable by EPR Spectroscopy

  1997cited by this paper
• Biological activation of hydrogen.

  1997cited by this paper
• Biological activition of hydrogen

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• INFRARED-SPECTROELECTROCHEMICAL CHARACTERIZATION OF THE NIFE HYDROGENASE OF DESULFOVIBRIO GIGAS

  1997cited by this paper
• Structure of the [Nife] Hydrogenase Active Site: Evidence for Biologically Uncommon Fe Ligands

  1996cited by this paper
• The NAD-linked soluble hydrogenase from Alcaligenes eutrophus H16: detection and characterization of EPR signals deriving from nickel and flavin

  1996cited by this paper
• The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling

  1996cited by this paper
• Structure of the Ni sites in hydrogenases by X-ray absorption spectroscopy. Species variation and the effects of redox poise

  1996cited by this paper
• Infrared-detectable groups sense changes in charge density on the nickel center in hydrogenase from Chromatium vinosum.

  1995cited by this paper
• FMN cofactor dissociation from the soluble hydrogenase of Alcaligenes eutrophus H16.

  1995cited by this paper
• Crystal structure of the nickel–iron hydrogenase from Desulfovibrio gigas

  1995cited by this paper
• Infrared studies on the interaction of carbon monoxide with divalent nickel in hydrogenase from Chromatium vinosum.

  1994cited by this paper
• Hydrogenase mutants of Alcaligenes eutrophus H16 show alterations in the electron transport system.

  1992cited by this paper
• Distinct redox behaviour of prosthetic groups in ready and unready hydrogenase from Chromatium vinosum.

  1992cited by this paper
• On the redox equilibrium between H2 and hydrogenase.

  1992cited by this paper
• Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16

  1990cited by this paper
• Redox-linked conformational changes in proteins detected by a combination of infrared spectroscopy and protein electrochemistry. Evaluation of the technique with cytochrome c.

  1990cited by this paper
• Effect of 17O2 and 13CO on EPR spectra of nickel in hydrogenase from Chromatium vinosum.

  1990cited by this paper
• Redox-dependent inactivation of the NAD-dependent hydrogenase from Alcaligenes eutrophus Z1.

  1989cited by this paper
• Redox behaviour of nickel in hydrogenase from Methanobacterium thermoautotrophicum (strain Marburg). Correlation between the nickel valence state and enzyme activity

  1989cited by this paper
• Isolation and immunological characterization of the four non-identical subunits of the soluble NAD-linked hydrogenase from Alcaligenes eutrophus H16.

  1986cited by this paper
• EPR evidence for direct interaction of carbon monoxide with nickel in hydrogenase from Chromatium vinosum

  1986cited by this paper
• Activation and deactivation of the membrane-bound hydrogenase from Desulfovibrio desulfuricans, Norway strain.

  1986cited by this paper
• Properties and reactivation of two different deactivated forms of Desulfovibrio gigas hydrogenase

  1985cited by this paper
• Monovalent nickel in hydrogenase from Chromatium vinosum

  1985cited by this paper
• Purification of hydrogenases by affinity chromatography on Procion Red-agarose.

  1983cited by this paper
• New insights, ideas and unanswered questions concerning iron-sulfur clusters in mitochondria.

  1982cited by this paper
• In vivo inactivation of soluble hydrogenase of Alcaligenes eutrophus

  1981cited by this paper
• Production of superoxide radicals by soluble hydrogenase from Alcaligenes eutrophus H16.

  1981cited by this paper
• Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus

  1981cited by this paper
• The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus.

  1979cited by this paper
• The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties.

  1979cited by this paper
• Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.

  1979cited by this paper
• Localization and stability of hydrogenases from aerobic hydrogen bacteria

  1977cited by this paper
• Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16.

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• Respiratory components and oxidase activities in Alcaligenes eutrophus.

  1976cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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  2023cites this paper
• Understanding 2D-IR Spectra of Hydrogenases: A Descriptive and Predictive Computational Study

  2022cites this paper
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  2018cites this paper
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  2016influential citation
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  2015cites this paper
• Concepts in bio-molecular spectroscopy: vibrational case studies on metalloenzymes.

  2015cites this paper
• Reversible active site sulfoxygenation can explain the oxygen tolerance of a NAD+-reducing [NiFe] hydrogenase and its unusual infrared spectroscopic properties.

  2015cites this paper
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  2013cites this paper
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