The Hydrogenase Subcomplex of the NAD+‐Reducing [NiFe] Hydrogenase from Ralstonia eutropha – Insights into Catalysis and Redox Interconversions

No abstract is available for this paper.

• Publication year

  2011

• Venue

  European Journal of Inorganic Chemistry

• Publication date

  2011-03-01

• Fields of study

  Biology, Chemistry, Environmental Science

• Identifiers
  DOI 10.1002/EJIC.201001053
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• "Two-step" chronoamperometric method for studying the anaerobic inactivation of an oxygen tolerant NiFe hydrogenase.

  2010cited by this paper
• Requirements for Construction of a Functional Hybrid Complex of Photosystem I and [NiFe]-Hydrogenase

  2010cited by this paper
• Probing the active site of an O2-tolerant NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 by in situ EPR and FTIR spectroscopy.

  2010cited by this paper
• Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.

  2010cited by this paper
• Membrane-bound hydrogenase I from the hyperthermophilic bacterium Aquifex aeolicus: enzyme activation, redox intermediates and oxygen tolerance.

  2010cited by this paper
• Structural Basis for the Mechanism of Respiratory Complex I*

  2009cited by this paper
• Spectroelectrochemical study of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F in solution and immobilized on biocompatible gold surfaces.

  2009cited by this paper
• How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms

  2009cited by this paper
• Overexpression, Isolation, and Spectroscopic Characterization of the Bidirectional [NiFe] Hydrogenase from Synechocystis sp. PCC 6803*

  2009cited by this paper
• Photosynthetic hydrogen production by a hybrid complex of photosystem I and [NiFe]-hydrogenase.

  2009cited by this paper
• The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy.

  2009cited by this paper
• Spectroscopic investigation of flavoproteins: Mechanistic differences between (electro)chemical and photochemical reduction and oxidation

  2008cited by this paper
• Activation and inactivation of hydrogenase function and the catalytic cycle: spectroelectrochemical studies.

  2007cited by this paper
• How Escherichia coli Is Equipped to Oxidize Hydrogen under Different Redox Conditions*

  2007cited by this paper
• [NiFe] and [FeFe] hydrogenases studied by advanced magnetic resonance techniques.

  2007cited by this paper
• Investigating and exploiting the electrocatalytic properties of hydrogenases.

  2007cited by this paper
• Occurrence, classification, and biological function of hydrogenases: an overview.

  2007cited by this paper
• [NiFe]-Hydrogenases of Ralstonia eutropha H16: Modular Enzymes for Oxygen-Tolerant Biological Hydrogen Oxidation

  2006cited by this paper
• Electricity from low-level H2 in still air--an ultimate test for an oxygen tolerant hydrogenase.

  2006cited by this paper
• Rapid and reversible reactions of [NiFe]-hydrogenases with sulfide.

  2006cited by this paper
• An improved purification procedure for the soluble [NiFe]-hydrogenase of Ralstonia eutropha: new insights into its (in)stability and spectroscopic properties

  2006cited by this paper
• Activation process of [NiFe] hydrogenase elucidated by high-resolution X-ray analyses: conversion of the ready to the unready state.

  2005cited by this paper
• Structural differences between the ready and unready oxidized states of [NiFe] hydrogenases

  2005cited by this paper
• Electrochemical definitions of O2 sensitivity and oxidative inactivation in hydrogenases.

  2005cited by this paper
• The Soluble NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH

  2005cited by this paper
• Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels.

  2005cited by this paper
• Reduction of Unusual Iron-Sulfur Clusters in the H2-sensing Regulatory Ni-Fe Hydrogenase from Ralstonia eutropha H16*

  2005cited by this paper
• Structural and oxidation-state changes at its nonstandard Ni-Fe site during activation of the NAD-reducing hydrogenase from Ralstonia eutropha detected by X-ray absorption, EPR, and FTIR spectroscopy.

  2005cited by this paper
• Electrochemical potential-step investigations of the aerobic interconversions of [NiFe]-hydrogenase from Allochromatium vinosum: insights into the puzzling difference between unready and ready oxidized inactive states.

  2004cited by this paper
• Selective release and function of one of the two FMN groups in the cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha.

  2004cited by this paper
• The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen

  2004cited by this paper
• Direct detection of a hydrogen ligand in the [NiFe] center of the regulatory H2-sensing hydrogenase from Ralstonia eutropha in its reduced state by HYSCORE and ENDOR spectroscopy.

  2003cited by this paper
• X-ray absorption spectroscopy to analyze nuclear geometry and electronic structure of biological metal centers—potential and questions examined with special focus on the tetra-nuclear manganese complex of oxygenic photosynthesis

  2003cited by this paper
• Unusual FTIR and EPR properties of the H2‐activating site of the cytoplasmic NAD‐reducing hydrogenase from Ralstonia eutropha

  2000cited by this paper
• Transcriptional Regulation of Alcaligenes eutrophus Hydrogenase Genes

  1998cited by this paper
• Crystal structure of the nickel–iron hydrogenase from Desulfovibrio gigas

  1995cited by this paper
• Multiple-scattering calculations of x-ray-absorption spectra.

  1995cited by this paper
• The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation

  1994cited by this paper
• Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16

  1990cited by this paper
• Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16

  1986cited by this paper
• Properties and reactivation of two different deactivated forms of Desulfovibrio gigas hydrogenase

  1985cited by this paper
• Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca 1b.

  1984cited by this paper
• Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1 b.

  1984cited by this paper
• Semiquinone formation in flavo- and metalloflavoproteins.

  1983cited by this paper
• Measurement of the extinction coefficient of the methyl viologen cation radical and the efficiency of its formation by semiconductor photocatalysis

  1982cited by this paper
• Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16.

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Estimation of the molecular weights of proteins by Sephadex gel-filtration.

  1964cited by this paper
• Hydrogenase: a bacterial enzyme activating molecular hydrogen: The properties of the enzyme.

  1931cited by this paper

• Biochemical and Mechanistic Insights into O2 Scavenging and Stability of the Soluble Hydrogenase from Hydrogenophaga pseudoflava

  2025cites this paper
• Pilot-scale application of sludge fermentation-driven nitrogen removal process with rapid in-situ enrichment of anammox bacteria for sustainable treatment of municipal wastewater and excess sludge

  2025cites this paper
• Efficient chemoautotrophic carbon fixation in controlled systems: Influencing factors, regulatory strategies and application prospects

  2025influential citation
• Reactivation and long-term stabilization of the [NiFe] Hox hydrogenase of Synechocystis sp. PCC6803 by glutathione after oxygen exposure

  2024cites this paper
• Functional roles of the [2Fe-2S] clusters in Synechocystis PCC 6803 Hox [NiFe]-hydrogenase reactivity with ferredoxins

  2024cites this paper
• Biocatalytic cofactor regeneration for CO2 reduction: Integration of a hydrogenase and a formate dehydrogenase in H2-driven systems

  2024cites this paper
• H2-driven biocatalysis for flavin-dependent ene-reduction in a continuous closed-loop flow system utilizing H2 from water electrolysis

  2024cites this paper
• Understanding 2D-IR Spectra of Hydrogenases: A Descriptive and Predictive Computational Study

  2022cites this paper
• The dyad of the Y-junction- and a flavin module unites diverse redox enzymes.

  2021cites this paper
• Energetics, electron uptake mechanisms and limitations of electroautotrophs growing on biocathodes - A review.

  2021cites this paper
• Electrochemical characterization of a truncated hydrogenase from Pyrococcus furiosus

  2021cites this paper
• Powering biocatalytical reactions with hydrogen

  2020cites this paper
• Advanced nonlinear gas phase control for autotrophic cultivations of different mutants of Ralstonia eutropha

  2020cites this paper
• Spectroscopic studies on hydrogenases

  2020cites this paper
• H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions.

  2020cites this paper
• The structure and reactivity of the HoxEFU complex from the cyanobacterium Synechocystis sp. PCC 6803

  2020cites this paper
• NAD+‐Reducing [NiFe]‐Hydrogenase

  2019cites this paper
• L'hydrogène moléculaire en tant que modulateur des communautés microbiennes de sol.

  2019cites this paper
• Molecular Hydrogen, a Neglected Key Driver of Soil Biogeochemical Processes

  2019cites this paper
• Hydrogenase

  2019cites this paper
• Advanced nonlinear multi-layer process control for autotrophic cultivations

  2019cites this paper
• Understanding the structure and dynamics of hydrogenases by ultrafast and two-dimensional infrared spectroscopy† †Electronic supplementary information (ESI) available: Supplementary figures. Description of the Morse fit. Analysis of CN stretch vibrational relaxation. References. See DOI: 10.1039/c9s

  2019cites this paper
• Enzymatic and spectroscopic properties of a thermostable [NiFe]‑hydrogenase performing H2-driven NAD+-reduction in the presence of O2.

  2018cites this paper
• Enzymes as modular catalysts for redox half-reactions in H2-powered chemical synthesis: from biology to technology

  2017cites this paper
• New perspectives in hydrogenase direct electrochemistry

  2017cites this paper
• Function of triazenido compound for electrocatalytic hydrogen production catalyzed by platinum complex

  2016cites this paper
• Function of 7,7,8,8-tetracyanoquinodimethane (TCNQ) on electrocatalytic hydrogen generation catalyzed by N,N′-benzene bis(salicylideniminato)nickel(II)

  2016cites this paper
• Modelling the production of soluble hydrogenase in Ralstonia eutropha by on-line optimal experimental design*

  2016cites this paper
• A flexible toolbox to study protein‐assisted metalloenzyme assembly in vitro

  2015cites this paper
• Active Site of the NAD(+)-Reducing Hydrogenase from Ralstonia eutropha Studied by EPR Spectroscopy.

  2015cites this paper
• Crystallization and preliminary X-ray analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1.

  2015cites this paper
• Impact of the iron-sulfur cluster proximal to the active site on the catalytic function of an O2-tolerant NAD(+)-reducing [NiFe]-hydrogenase.

  2015cites this paper
• Reversible active site sulfoxygenation can explain the oxygen tolerance of a NAD+-reducing [NiFe] hydrogenase and its unusual infrared spectroscopic properties.

  2015cites this paper
• Enzyme-Modified Particles for Selective Biocatalytic Hydrogenation by Hydrogen-Driven NADH Recycling

  2015cites this paper
• Model-based control to maximise biomass and PHB in the autotrophic cultivation of Ralstonia eutropha

  2015cites this paper
• The [NiFe]-Hydrogenase of Pyrococcus furiosus Exhibits a New Type of Oxygen Tolerance.

  2015cites this paper
• Concepts in bio-molecular spectroscopy: vibrational case studies on metalloenzymes.

  2015cites this paper
• The Investigation and Characterization of Redox Enzymes Using Protein Film Electrochemistry

  2014cites this paper
• Bioelectrocatalysis of Hydrogen Oxidation/Reduction by Hydrogenases

  2014cites this paper
• Proteinbiochemische, spektroskopische und röntgenkristallographische Untersuchung der Actinobakteriellen [NiFe]-Hydrogenase aus Ralstonia eutropha

  2014influential citation
• Structure, function and biosynthesis of O2-tolerant hydrogenases

  2013cites this paper
• Biohydrogen as a fuel : understanding and engineering hydrogenase enzymes for biotechnological applications

  2013cites this paper
• H2-Metabolizing Prokaryotes

  2013cites this paper
• Stability enhancement of an O2-tolerant NAD+-reducing [NiFe]-hydrogenase by a combination of immobilisation and chemical modification

  2013cites this paper
• H2‐driven cofactor regeneration with NAD(P)+‐reducing hydrogenases

  2013cites this paper
• [NiFe] hydrogenases: a common active site for hydrogen metabolism under diverse conditions.

  2013cites this paper
• An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli

  2013cites this paper
• Catalytic production of hydrogen peroxide and water by oxygen-tolerant [NiFe]-hydrogenase during H2 cycling in the presence of O2.

  2013cites this paper
• A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties.

  2012cites this paper
• Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants

  2012cites this paper
• Stabilisation of the NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 through modification with methoxy-poly(ethylene) glycol

  2012cites this paper
• EPR spectroscopic studies of the Fe-S clusters in the O2-tolerant [NiFe]-hydrogenase Hyd-1 from Escherichia coli and characterization of the unique [4Fe-3S] cluster by HYSCORE.

  2012cites this paper
• NAD(H)‐coupled hydrogen cycling – structure–function relationships of bidirectional [NiFe] hydrogenases

  2012cites this paper
• The [NiFe]-hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 works bidirectionally with a bias to H2 production.

  2011cites this paper
• Catalytic Properties of the Isolated Diaphorase Fragment of the NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha

  2011cites this paper
• Systematic evaluation of the dihydrogen-oxidising and NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 as a cofactor regeneration catalyst

  2011influential citation