Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16.

No abstract is available for this paper.

• Publication year

  1976

• Venue

  Biochimica et Biophysica Acta

• Publication date

  1976-11-08

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/0005-2744(76)90058-9PMID 186126
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Studies on a gram-positive hydrogen bacterium, Nocardia opaca 1 b

  2004cited by this paper
• Structural and catalytic properties of hydrogenase from Chromatium.

  1975cited by this paper
• Purification and properties of hydrogenase from Clostridium pasteurianum W5.

  1974cited by this paper
• Description and physiological characterization of a coryneform hydrogen bacterium, strain 14 g

  1973cited by this paper
• Some Properties of Formate Dehydrogenase

  1972cited by this paper
• An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue.

  1972cited by this paper
• The acceptor specificity of flavins and flavoproteins. 3. Flavoproteins.

  1971cited by this paper
• Evidence for the involvement of non-heme iron in the active site of hydrogenase from Desulfovibrio vulgaris.

  1971cited by this paper
• Purification and properties of hydrogenase, an iron sulfur protein, from Clostridium pasteurianum W5.

  1971cited by this paper
• Structural properties of hydrogenase from Clostridium pasteurianum W5.

  1971cited by this paper
• Purification and Properties of a Hydrogenase from Desulfovibrio vulgaris

  1971cited by this paper
• [Properties of the NAD-specific hydrogenase from Hydrogenomonas H 16].

  1970cited by this paper
• The effect or ultraviolet light on the hydrogenase of Proteus vulgaris.

  1968cited by this paper
• Multiple Forms of Bacterial Hydrogenases

  1966cited by this paper
• [On the hydrogen-activating system of Hydrogenomonas H 16. I. Distribution of the hydrogenase activity between two cellular fractions].

  1966cited by this paper
• IRON REQUIREMENT FOR THE HYDROGENASE OF DESULFOVIBRIO DESULFURICANS.

  1964cited by this paper
• Estimation of the molecular weights of proteins by Sephadex gel-filtration.

  1964cited by this paper
• DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS *

  1964cited by this paper
• Studies on the respiratory chain-linked reduced nicotinamide adenine dinucleotide dehydrogenase. II. Isolation and molecular properties of the enzyme from beef heart.

  1963cited by this paper
• Purification and some properties of different forms of hydrogen dehydrogenase.

  1963cited by this paper
• Inhibitor, isotopic and kinetic studies on hydrogen dehydrogenase.

  1963cited by this paper
• The electron transport system of Hydrogenomonas eutropha. I. Diphosphopyridine nucleotide reduction by hydrogen.

  1962cited by this paper
• Symposium on autotrophy. V. Carbon dioxide fixation and substrate oxidation in the chemosynthetic sulfur and hydrogen bacteria.

  1962cited by this paper
• Purification and properties of the hydrogenase of Desulfovibrio desulfuricans.

  1961cited by this paper
• A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

  1961cited by this paper
• Studies on hydrogen oxidation in cell-free extracts of Hydrogenomonas eutropha.

  1961cited by this paper
• [A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies].

  1961cited by this paper
• Localization of hydrogen activating enzymes in Pseudomonas saccharophila.

  1960cited by this paper
• Studies on succinic dehydrogenase. II. Isolation and properties of the dehydrogenase from beef heart.

  1957cited by this paper
• Purification and properties of the hydrogenase of Desulfovibrio desulfuricans.

  1956cited by this paper
• The specificity of a diphosphopyridine nucleotide-linked hydrogenase.

  1955cited by this paper
• Oxidation and evolution of molecular hydrogen by microorganisms.

  1954cited by this paper
• The biochemistry of Hydrogenomonas. I. The hydrogenase of Hydrogenomonas facilis in cell-free preparations.

  1954cited by this paper
• The interaction of hydrogenase with oxygen.

  1954cited by this paper
• Protein measurement with the Folin phenol reagent.

  1951cited by this paper
• Hydrogenase: a bacterial enzyme activating molecular hydrogen: The properties of the enzyme.

  1931cited by this paper

• Biochemical and Mechanistic Insights into O2 Scavenging and Stability of the Soluble Hydrogenase from Hydrogenophaga pseudoflava

  2025cites this paper
• Co-expression of auxiliary genes enhances the activity of a heterologous O2-tolerant hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803

  2025cites this paper
• Diverse lineages and adaptations of oxygen-adapted hydrogenases.

  2025cites this paper
• Engineering O2-Tolerant Chimeric Hydrogenases Optimized for Ferredoxin Coupling in Synechocystis sp. PCC 6803.

  2025cites this paper
• Development of a Universal Platform for the Heterologous Expression of Bidirectional [Ni–Fe]-Hydrogenases in

  2025cites this paper
• Hydrogen-driven, ATP-dependent biocatalytic reduction of carboxylic acids under non-explosive conditions

  2025cites this paper
• Biocatalytic cofactor regeneration for CO2 reduction: Integration of a hydrogenase and a formate dehydrogenase in H2-driven systems

  2024cites this paper
• The energy metabolism of Cupriavidus necator in different trophic conditions

  2024cites this paper
• Exploiting hydrogenases for biocatalytic hydrogenations

  2024cites this paper
• Hydrogenase-based oxidative biocatalysis without oxygen

  2023cites this paper
• Gallionellaceae pangenomic analysis reveals insight into phylogeny, metabolic flexibility, and iron oxidation mechanisms

  2023cites this paper
• Improved preculture management for Cupriavidus necator cultivations

  2023cites this paper
• A Hitchhiker’s Guide to Supplying Enzymatic Reducing Power into Synthetic Cells

  2023cites this paper
• Evidence for Electron Transfer from the Bidirectional Hydrogenase to the Photosynthetic Complex I (NDH-1) in the Cyanobacterium Synechocystis sp. PCC 6803

  2022cites this paper
• Unique H2-utilizing lithotrophy in serpentinite-hosted systems

  2022cites this paper
• [NiFe] Hydrogenase Accessory Proteins HypB-HypC Accelerate Proton Conversion to Enhance the Acid Resistance and d-Lactic Acid Production of Escherichia coli.

  2022cites this paper
• Hydrogen Oxidizing Bacteria as Novel Protein Source for Human Consumption: An Overview

  2022cites this paper
• Rewiring cyanobacterial photosynthesis by the implementation of an oxygen-tolerant hydrogenase.

  2021cites this paper
• Unique metabolic strategies in Hadean analogues reveal hints for primordial physiology

  2021cites this paper
• Metabolic strategies of dormancy of a marine bacterium Microbulbifer aggregans CCB-MM1: Its alternative electron transfer chain and sulfate-reducing pathway.

  2021cites this paper
• E. coli Nickel‐Iron Hydrogenase 1 Catalyses Non‐native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene‐reductases

  2021cites this paper
• E. coli Nickel‐Iron Hydrogenase 1 Catalyses Non‐native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene‐reductases

  2021cites this paper
• Spectroscopic studies on hydrogenases

  2020cites this paper
• H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions.

  2020cites this paper
• Dihydrogen‐Driven NADPH Recycling in Imine Reduction and P450‐Catalyzed Oxidations Mediated by an Engineered O2‐Tolerant Hydrogenase

  2020cites this paper
• Discriminating changes in intracellular NADH/NAD+ levels due to anoxicity and H2 supply in R. eutropha cells using the Frex fluorescence sensor.

  2019cites this paper
• How to make the reducing power of H2 available for in vivo biosyntheses and biotransformations.

  2019cites this paper
• NAD+‐Reducing [NiFe]‐Hydrogenase

  2019cites this paper
• Microbial Water Source for the Desert Plants

  2019cites this paper
• Structural Insights into the Protective Mechanisms against O2 in the [NiFe]-Hydrogenases

  2019cites this paper
• Enzymatic self-sufficient hydride transfer processes.

  2019cites this paper
• Unifying Activity, Structure, and Spectroscopy of [NiFe] Hydrogenases: Combining Techniques To Clarify Mechanistic Understanding.

  2019cites this paper
• Enzymatic and spectroscopic properties of a thermostable [NiFe]‑hydrogenase performing H2-driven NAD+-reduction in the presence of O2.

  2018cites this paper
• O2-tolerant [NiFe]-hydrogenases of Ralstonia eutropha H16: Physiology, molecular biology, purification, and biochemical analysis.

  2018cites this paper
• Minimal Influence of [NiFe] Hydrogenase on Hydrogen Isotope Fractionation in H2-Oxidizing Cupriavidus necator

  2017influential citation
• Structural basis of the redox switches in the NAD+-reducing soluble [NiFe]-hydrogenase

  2017cites this paper
• Artificial models of iron hydrogenases for their potential use in the generation of molecular hydrogen: a mini-review

  2017cites this paper
• Investigation of the NADH/NAD+ ratio in Ralstonia eutropha using the fluorescence reporter protein Peredox.

  2017cites this paper
• Polarized potential and electrode materials implication on electro-fermentative di-hydrogen production: Microbial assemblages and hydrogenase gene copy variation.

  2016cites this paper
• A HupSL és Hox1 NiFe hidrogenáz enzimek összehangolt szabályozásának vizsgálata Thiocapsa roseopersicina baktériumban

  2016cites this paper
• Role of Iron Concentration on Hydrogen Production Using Confectionery Wastewater

  2016cites this paper
• Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications

  2016cites this paper
• Asymmetric Biocatalytic Amination of Ketones at the Expense of NH3 and Molecular Hydrogen.

  2015cites this paper
• An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture

  2015cites this paper
• A flexible toolbox to study protein‐assisted metalloenzyme assembly in vitro

  2015cites this paper
• Concepts in bio-molecular spectroscopy: vibrational case studies on metalloenzymes.

  2015cites this paper
• Crystallization and preliminary X-ray analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1.

  2015cites this paper
• Electrochemical and Infrared Spectroscopic Studies Provide Insight into Reactions of the NiFe Regulatory Hydrogenase from Ralstonia eutropha with O2 and CO.

  2015cites this paper
• The [NiFe]-Hydrogenase of Pyrococcus furiosus Exhibits a New Type of Oxygen Tolerance.

  2015cites this paper
• Impact of the iron-sulfur cluster proximal to the active site on the catalytic function of an O2-tolerant NAD(+)-reducing [NiFe]-hydrogenase.

  2015cites this paper
• Synthesis of nickel-iron hydrogenase in Cupriavidus metallidurans is controlled by metal-dependent silencing and un-silencing of genomic islands.

  2015cites this paper
• Reversible active site sulfoxygenation can explain the oxygen tolerance of a NAD+-reducing [NiFe] hydrogenase and its unusual infrared spectroscopic properties.

  2015cites this paper
• Active Site of the NAD(+)-Reducing Hydrogenase from Ralstonia eutropha Studied by EPR Spectroscopy.

  2015cites this paper
• The Investigation and Characterization of Redox Enzymes Using Protein Film Electrochemistry

  2014cites this paper
• Proteinbiochemische, spektroskopische und röntgenkristallographische Untersuchung der Actinobakteriellen [NiFe]-Hydrogenase aus Ralstonia eutropha

  2014influential citation
• The Growth and Survival of Mycobacterium smegmatis Is Enhanced by Co-Metabolism of Atmospheric H2

  2014cites this paper
• Physiological roles of the three [NiFe]-hydrogenases in Mycobacterium smegmatis

  2014cites this paper
• The importance of iron in the biosynthesis and assembly of [NiFe]-hydrogenases

  2014cites this paper
• H2-Metabolizing Prokaryotes

  2013cites this paper
• Strategies for Cofactor Regeneration in Biocatalyzed Reductions

  2013cites this paper
• H2‐driven cofactor regeneration with NAD(P)+‐reducing hydrogenases

  2013cites this paper
• Structure, function and biosynthesis of O2-tolerant hydrogenases

  2013cites this paper
• Combining spectroscopy and theory to evaluate structural models of metalloenzymes: a case study on the soluble [NiFe] hydrogenase from Ralstonia eutropha.

  2013cites this paper
• Novel, Oxygen-Insensitive Group 5 [NiFe]-Hydrogenase in Ralstonia eutropha

  2013cites this paper
• An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli

  2013cites this paper
• Fundamentals and electrochemical applications of [Ni–Fe]-uptake hydrogenases

  2013cites this paper
• Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon

  2013influential citation
• Contrôle de réaction catalysée par une oxydoréductase et mise en oeuvre de celle-ci

  2013cites this paper
• Biohydrogen as a fuel : understanding and engineering hydrogenase enzymes for biotechnological applications

  2013cites this paper
• Engineering ralstonia eutropha for production of isobutanol from CO2, H2, and O2

  2013cites this paper
• Syngas fermentation to biofuels: Effects of hydrogen partial pressure on hydrogenase efficiency

  2013cites this paper
• Catalytic production of hydrogen peroxide and water by oxygen-tolerant [NiFe]-hydrogenase during H2 cycling in the presence of O2.

  2013cites this paper
• Stability enhancement of an O2-tolerant NAD+-reducing [NiFe]-hydrogenase by a combination of immobilisation and chemical modification

  2013cites this paper
• NAD(H)‐coupled hydrogen cycling – structure–function relationships of bidirectional [NiFe] hydrogenases

  2012cites this paper
• Stabilisation of the NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 through modification with methoxy-poly(ethylene) glycol

  2012cites this paper
• A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties.

  2012cites this paper
• Catalytic Properties of the Isolated Diaphorase Fragment of the NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha

  2011cites this paper
• Systematic evaluation of the dihydrogen-oxidising and NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 as a cofactor regeneration catalyst

  2011influential citation
• The Hydrogenase Subcomplex of the NAD+‐Reducing [NiFe] Hydrogenase from Ralstonia eutropha – Insights into Catalysis and Redox Interconversions

  2011cites this paper
• Metabolic versatility of (NiFe)- hydrogenases in Thiocapsa roseopersicina

  2010cites this paper
• Regeneration of nicotinamide coenzymes: principles and applications for the synthesis of chiral compounds.

  2010cites this paper
• Hydrogenases and hydrogen metabolism in photosynthetic prokaryotes.

  2010cites this paper
• Protein-protein complex formation affects the Ni-Fe and Fe-S centers in the H2-sensing regulatory hydrogenase from Ralstonia eutropha H16.

  2010cites this paper
• Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production

  2010cites this paper
• Utilization of protein-rich animal waste materials to produce biohydrogen

  2009cites this paper
• Megaplasmids of Aerobic Hydrogenotrophic and Carboxidotrophic Bacteria

  2009influential citation
• Analyse des [NiFe]-Zentrums und der Kofaktoren im H 2-Sensor von Ralstonia eutropha H16

  2008cites this paper
• Identification of major increased T-RFs in T-RFLP profiles of hydrogen treated soil samples using molecular fingerpr[in]ting techniques

  2008cites this paper
• Hydrogenases and H(+)-reduction in primary energy conservation.

  2008cites this paper
• Hydrogenases from the Hyperthermophilic Archaeon Pyrococcus furiosus

  2007cites this paper
• Fermentation of biomass‐generated synthesis gas: Effects of nitric oxide

  2007influential citation
• Reinvestigation of the Steady-State Kinetics and Physiological Function of the Soluble NiFe-Hydrogenase I of Pyrococcus furiosus

  2007cites this paper
• Structure based mechanistic studies on 2-ketopropyl coenzyme M oxidoreductase / carboxylase from Xanthobacter autotrophicus and [FeFe] hydrogenase from Clostridium pasteurianum

  2007cites this paper
• Inhibition of respiration and nitrate assimilation enhances photohydrogen evolution under low oxygen concentrations in Synechocystis sp. PCC 6803.

  2007cites this paper
• Chaperones specific for the membrane‐bound [NiFe]‐hydrogenase interact with the Tat signal peptide of the small subunit precursor in Ralstonia eutropha H16

  2007influential citation
• Investigation of Flavoproteins Involved in the Metabolism of Anaerobic Hyperthermophilic Microorganisms

  2007cites this paper
• Occurrence, classification, and biological function of hydrogenases: an overview.

  2007cites this paper
• An improved purification procedure for the soluble [NiFe]-hydrogenase of Ralstonia eutropha: new insights into its (in)stability and spectroscopic properties

  2006cites this paper
• Bias from H2 cleavage to production and coordination changes at the Ni-Fe active site in the NAD+-reducing hydrogenase from Ralstonia eutropha.

  2006cites this paper
• [NiFe]-Hydrogenases of Ralstonia eutropha H16: Modular Enzymes for Oxygen-Tolerant Biological Hydrogen Oxidation

  2006cites this paper