Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

Transportin-specific molecular tools are used to show that the mitotic cell contains importin β and transportin “global positioning system” pathways that are mechanistically parallel. Transportin works to control where the spindle, nuclear membrane, and nuclear pores are formed by directly affecting assembly factor function.

• Publication year

  2014

• Venue

  Molecular Biology of the Cell

• Publication date

  2014-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E13-08-0506PMID 24478460PMCID 3967982
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Enclosing chromatin: reassembly of the nucleus after open mitosis.

  2013cited by this paper
• RanGTP and CLASP1 cooperate to position the mitotic spindle

  2013influential reference
• Identification of Cargo Proteins Specific for Importin-β with Importin-α Applying a Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based in Vitro Transport System*

  2013cited by this paper
• Structural and functional studies of the 252 kDa nucleoporin ELYS reveal distinct roles for its three tethered domains.

  2013cited by this paper
• Midbody assembly and its regulation during cytokinesis

  2012cited by this paper
• Structural and energetic basis of ALS-causing mutations in the atypical proline–tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS)

  2012cited by this paper
• Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex

  2012cited by this paper
• Nup98 regulates bipolar spindle assembly through association with microtubules and opposition of MCAK

  2011cited by this paper
• Coordinating postmitotic nuclear pore complex assembly with abscission timing

  2011cited by this paper
• Importazole, a small molecule inhibitor of the transport receptor importin-β.

  2011cited by this paper
• Nuclear import by karyopherin-βs: recognition and inhibition.

  2011influential reference
• The nuclear pore complex and nuclear transport.

  2010cited by this paper
• Recognition of nuclear targeting signals by Karyopherin-β proteins.

  2010cited by this paper
• ALS‐associated fused in sarcoma (FUS) mutations disrupt Transportin‐mediated nuclear import

  2010cited by this paper
• Defects in nuclear pore assembly lead to activation of an Aurora B–mediated abscission checkpoint

  2010cited by this paper
• The Nup107-160 complex and γ-TuRC regulate microtubule polymerization at kinetochores

  2010cited by this paper
• The Part and the Whole: functions of nucleoporins in nucleocytoplasmic transport.

  2010cited by this paper
• Small molecule peptidomimetic inhibitors of importin α/β mediated nuclear transport.

  2010cited by this paper
• Transportin Mediates Nuclear Entry of DNA in Vertebrate Systems

  2009cited by this paper
• Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.

  2009influential reference
• Importin beta regulates the seeding of chromatin with initiation sites for nuclear pore assembly.

  2009cited by this paper
• Learning about cancer from frogs: analysis of mitotic spindles in Xenopus egg extracts

  2009cited by this paper
• Karyopherin binding interactions and nuclear import mechanism of nuclear pore complex protein Tpr

  2009cited by this paper
• Spatial and temporal coordination of mitosis by Ran GTPase

  2008influential reference
• Xenopus importin beta validates human importin beta as a cell cycle negative regulator

  2008influential reference
• Classical NLS proteins from Saccharomyces cerevisiae.

  2008cited by this paper
• Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rate

  2008influential reference
• Biology and biophysics of the nuclear pore complex and its components.

  2008cited by this paper
• Reorganization of the nuclear envelope during open mitosis.

  2008cited by this paper
• Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.

  2008cited by this paper
• In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

  2008influential reference
• Structural and functional studies of Nup107/Nup133 interaction and its implications for the architecture of the nuclear pore complex.

  2008cited by this paper
• Modular Organization and Combinatorial Energetics of Proline–Tyrosine Nuclear Localization Signals

  2008cited by this paper
• The RanGTP gradient – a GPS for the mitotic spindle

  2008influential reference
• MEL‐28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly

  2007influential reference
• Conformational heterogeneity of karyopherin beta2 is segmental.

  2007cited by this paper
• Pin1 stabilizes Emi1 during G2 phase by preventing its association with SCFβtrcp

  2007cited by this paper
• The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivo.

  2007cited by this paper
• Conformational Heterogeneity of Karyopherinβ2 Is Segmental

  2007influential reference
• Spatial control of mitosis by the GTPase Ran

  2007influential reference
• Structure-based design of a pathway-specific nuclear import inhibitor

  2007influential reference
• The human Nup107–160 nuclear pore subcomplex contributes to proper kinetochore functions

  2007cited by this paper
• The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly.

  2006influential reference
• FG-Rich Repeats of Nuclear Pore Proteins Form a Three-Dimensional Meshwork with Hydrogel-Like Properties

  2006cited by this paper
• MEL-28, a novel nuclear-envelope and kinetochore protein essential for zygotic nuclear-envelope assembly in C. elegans.

  2006cited by this paper
• The nuclear envelope: form and reformation.

  2006cited by this paper
• Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts.

  2006influential reference
• Analysis of a RanGTP-regulated gradient in mitotic somatic cells

  2006cited by this paper
• NuSAP, a mitotic RanGTP target that stabilizes and cross-links microtubules.

  2006cited by this paper
• ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division

  2006influential reference
• A Mitotic Lamin B Matrix Induced by RanGTP Required for Spindle Assembly

  2006cited by this paper
• In vitro study of nuclear assembly and nuclear import using Xenopus egg extracts.

  2006influential reference
• Rules for nuclear localization sequence recognition by karyopherin beta 2.

  2006cited by this paper
• Two motifs essential for nuclear import of the hnRNP A1 nucleocytoplasmic shuttling sequence M9 core

  2006cited by this paper
• Karyopherin flexibility in nucleocytoplasmic transport.

  2006cited by this paper
• Proteome Analysis of the Human Mitotic Spindle*S

  2005cited by this paper
• Importin alpha/beta and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal.

  2004cited by this paper
• Proteome analysis of the human mitotic spindle

  2004cited by this paper
• Minimal nuclear pore complexes define FG repeat domains essential for transport

  2004cited by this paper
• Importin α associates with membranes and participates in nuclear envelope assembly in vitro

  2004cited by this paper
• Transportins 1 and 2 are redundant nuclear import factors for hnRNP A1 and HuR.

  2004cited by this paper
• Karyopherins: from nuclear-transport mediators to nuclear-function regulators.

  2004cited by this paper
• Mitotic Functions of the Ran GTPase Network: the Importance of Being in the Right Place at the Right Time

  2004cited by this paper
• Importin alpha: a multipurpose nuclear-transport receptor.

  2004cited by this paper
• Importin beta: conducting a much larger cellular symphony.

  2004influential reference
• Spatial and temporal control of nuclear envelope assembly by Ran GTPase.

  2004influential reference
• The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis.

  2004cited by this paper
• Importin α: A multipurpose nuclear-transport receptor

  2004cited by this paper
• The Ran GTPase regulates kinetochore function.

  2003influential reference
• A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo

  2003cited by this paper
• RanGTP mediates nuclear pore complex assembly

  2003cited by this paper
• Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.

  2003cited by this paper
• The Ran GTPase cycle is required for yeast nuclear pore complex assembly

  2003cited by this paper
• Nucleocytoplasmic transport: taking an inventory

  2003cited by this paper
• The conserved Nup107-160 complex is critical for nuclear pore complex assembly.

  2003cited by this paper
• The small GTPase Ran: interpreting the signs.

  2003influential reference
• Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.

  2003cited by this paper
• Complex Formation among the RNA Export Proteins Nup98, Rae1/Gle2, and TAP*

  2003cited by this paper
• Importin (cid:2) contains a COOH-terminal nucleoporin binding region important for nuclear transport

  2003cited by this paper
• Importin β contains a COOH-terminal nucleoporin binding region important for nuclear transport

  2003cited by this paper
• Visualization of a Ran-GTP Gradient in Interphase and Mitotic Xenopus Egg Extracts

  2002cited by this paper
• Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly.

  2002cited by this paper
• Role of importin-beta in the control of nuclear envelope assembly by Ran.

  2002cited by this paper
• Uncoupling Kapbeta2 substrate dissociation and ran binding.

  2002influential reference
• Role of Importin-β in the Control of Nuclear Envelope Assembly by Ran

  2002cited by this paper
• Proteomic analysis of the mammalian nuclear pore complex

  2002cited by this paper
• Nuclear Import Strategies of High Risk HPV16 L1 Major Capsid Protein*

  2002cited by this paper
• The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly

  2002cited by this paper
• Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells.

  2002cited by this paper
• Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.

  2002cited by this paper
• Uncoupling Kapβ2 Substrate Dissociation and Ran Binding

  2002influential reference
• The dynamics of karyopherin-mediated nuclear transport.

  2001cited by this paper
• A Link between the Synthesis of Nucleoporins and the Biogenesis of the Nuclear Envelope

  2001cited by this paper
• Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.

  2001cited by this paper
• Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly

  2001cited by this paper
• Importin β Is a Mitotic Target of the Small GTPase Ran in Spindle Assembly

  2001influential reference
• Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.

  2001cited by this paper
• An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells

  2001cited by this paper
• Running on Ran: nuclear transport and the mitotic spindle.

  2001cited by this paper
• Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities

  2001cited by this paper

• A Rare Missense Variant in TNPO2 in an Individual With a Neurodevelopmental Disability

  2025cites this paper
• Structure, function and assembly of nuclear pore complexes

  2025cites this paper
• Golgin45 assists mitosis via its nuclear localization sequence.

  2024cites this paper
• Therapeutic targeting of nuclear export and import receptors in cancer and their potential in combination chemotherapy

  2023cites this paper
• Puzzling out nuclear pore complex assembly

  2023cites this paper
• Nuclear transport proteins: structure, function and disease relevance

  2023cites this paper
• Non-transport roles of nuclear import receptors: In need of the right balance

  2022cites this paper
• The Nuclear Pore Complex: Birth, Life, and Death of a Cellular Behemoth

  2022cites this paper
• Attenuated Viral Replication of Avian Infectious Bronchitis Virus with a Novel 82-Nucleotide Deletion in the 5a Gene Indicates a Critical Role for 5a in Virus-Host Interactions

  2022cites this paper
• G-proteins | Ran GTPase

  2021cites this paper
• TNPO2 variants associate with human developmental delays, neurologic deficits, and dysmorphic features and alter TNPO2 activity in Drosophila.

  2021cites this paper
• Exportins can inhibit major mitotic assembly events in vitro: membrane fusion, nuclear pore formation, and spindle assembly

  2020influential citation
• Multiple components of the nuclear pore complex interact with the amino-terminus of MX2 to facilitate HIV-1 restriction

  2018cites this paper
• Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

  2018influential citation
• Regulation and Assembly of Actomyosin Contractile Rings in Cytokinesis and Cell Repair

  2018cites this paper
• Mitotic spindle assembly in animal cells: a fine balancing act

  2017cites this paper
• Mechanisms of nuclear pore complex assembly – two different ways of building one molecular machine

  2017cites this paper
• Targeting nuclear transporters in cancer: Diagnostic, prognostic and therapeutic potential

  2016cites this paper
• The RanGTP Pathway: From Nucleo-Cytoplasmic Transport to Spindle Assembly and Beyond

  2016cites this paper
• Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization.

  2015cites this paper
• The Ran Pathway in Drosophila melanogaster Mitosis

  2015cites this paper
• Purification of nuclear localization signal-containing proteins and its application to investigation of the mechanisms of the cell division cycle

  2015cites this paper
• Reprint of "Nuclear transport factors: global regulation of mitosis".

  2015cites this paper
• Nuclear localization signals for four distinct karyopherin-β nuclear import systems.

  2015cites this paper
• Nuclear transport factors: global regulation of mitosis.

  2015influential citation
• Thirty of search and capture: The complex simplicity of mitotic spindle assembly.

  2015cites this paper
• Thirty years of search and capture: The complex simplicity of mitotic spindle assembly

  2015cites this paper
• Transportin‐1 and Transportin‐2: Protein nuclear import and beyond

  2014influential citation