Chemical genetic screen for AMPKα2 substrates uncovers a network of proteins involved in mitosis.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Molecules and Cells

• Publication date

  2011-12-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.molcel.2011.11.005PMID 22137581PMCID PMC3246132
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A fluorescent reporter of AMPK activity and cellular energy stress.

  2011influential reference
• Class IIa histone deacetylases are hormone-activated regulators of FOXO and mammalian glucose homeostasis.

  2011cited by this paper
• Polo-like kinase 1 regulates activation of AMP-activated protein kinase (AMPK) at the mitotic apparatus

  2011cited by this paper
• Lifespan extension induced by AMPK and calcineurin is mediated by CRTC-1 and CREB

  2011cited by this paper
• The specificities of small molecule inhibitors of the TGFß and BMP pathways.

  2011cited by this paper
• Signaling Kinase AMPK Activates Stress-Promoted Transcription via Histone H2B Phosphorylation

  2010cited by this paper
• New Roles for the LKB1-NUAK Pathway in Controlling Myosin Phosphatase Complexes and Cell Adhesion

  2010influential reference
• Control of insulin granule dynamics by AMPK dependent KLC1 phosphorylation

  2009cited by this paper
• Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination

  2009cited by this paper
• The active form of the metabolic sensor AMP-activated protein kinase α (AMPKα) directly binds the mitotic apparatus and travels from centrosomes to the spindle midzone during mitosis and cytokinesis

  2009cited by this paper
• Mitotic kinase dynamics of the active form of AMPK (Phospho-AMPKαThr172) in human cancer cells

  2009cited by this paper
• The LKB1–AMPK pathway: metabolism and growth control in tumour suppression

  2009cited by this paper
• AMPK: Evidence for an energy-sensing cytokinetic tumor suppressor

  2009cited by this paper
• Global Analysis of Cdk1 Substrate Phosphorylation Sites Provides Insights into Evolution

  2009cited by this paper
• AMP-activated protein kinase phosphorylates retinoblastoma protein to control mammalian brain development.

  2009influential reference
• Myosin phosphatase target subunit: Many roles in cell function.

  2008cited by this paper
• Inhibition of ZAP-70 Kinase Activity via an Analog-sensitive Allele Blocks T Cell Receptor and CD28 Superagonist Signaling*S⃞

  2008cited by this paper
• Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates

  2008cited by this paper
• AMPK phosphorylation of raptor mediates a metabolic checkpoint.

  2008influential reference
• Energy-dependent regulation of cell structure by AMP-activated protein kinase

  2007cited by this paper
• A novel regulatory mechanism of myosin light chain phosphorylation via binding of 14-3-3 to myosin phosphatase.

  2007cited by this paper
• A semisynthetic epitope for kinase substrates

  2007influential reference
• An AMPK-FOXO pathway mediates longevity induced by a novel method of dietary restriction in C. elegans.

  2007influential reference
• Mad2 overexpression promotes aneuploidy and tumorigenesis in mice.

  2007cited by this paper
• Mechanism of Action of A-769662, a Valuable Tool for Activation of AMP-activated Protein Kinase*

  2007influential reference
• The selectivity of protein kinase inhibitors: a further update.

  2007cited by this paper
• Investigating the mechanism for AMP activation of the AMP-activated protein kinase cascade.

  2007cited by this paper
• The energy sensing LKB1–AMPK pathway regulates p27kip1 phosphorylation mediating the decision to enter autophagy or apoptosis

  2007cited by this paper
• Defining the Mechanism of Activation of AMP-activated Protein Kinase by the Small Molecule A-769662, a Member of the Thienopyridone Family*

  2007cited by this paper
• The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing.

  2007cited by this paper
• New candidate targets of AMP-activated protein kinase in murine brain revealed by a novel multidimensional substrate-screen for protein kinases.

  2007cited by this paper
• The Energy Sensor AMP-activated Protein Kinase Directly Regulates the Mammalian FOXO3 Transcription Factor*

  2007influential reference
• Recognizing and exploiting differences between RNAi and small-molecule inhibitors.

  2007cited by this paper
• Polo-Like Kinase Controls Vertebrate Spindle Elongation and Cytokinesis

  2007cited by this paper
• LKB1-dependent signaling pathways.

  2006cited by this paper
• Identification and characterization of a small molecule AMPK activator that treats key components of type 2 diabetes and the metabolic syndrome.

  2006cited by this paper
• 5′-AMP-Activated Protein Kinase (AMPK) Is Induced by Low-Oxygen and Glucose Deprivation Conditions Found in Solid-Tumor Microenvironments

  2006cited by this paper
• Chemical genetic analysis of the time course of signal transduction by JNK.

  2006cited by this paper
• The anaphase promoting complex/cyclosome: a machine designed to destroy

  2006cited by this paper
• AMP-activated protein kinase induces a p53-dependent metabolic checkpoint.

  2005cited by this paper
• AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism.

  2005cited by this paper
• The Kinase LKB1 Mediates Glucose Homeostasis in Liver and Therapeutic Effects of Metformin

  2005cited by this paper
• Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase.

  2005cited by this paper
• The CREB coactivator TORC2 is a key regulator of fasting glucose metabolism

  2005influential reference
• Bio-orthogonal affinity purification of direct kinase substrates.

  2005cited by this paper
• Cre-lox-regulated conditional RNA interference from transgenes.

  2004cited by this paper
• Genome-wide survey of protein kinases required for cell cycle progression

  2004cited by this paper
• The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans.

  2004cited by this paper
• Myosin phosphatase: Structure, regulation and function

  2004influential reference
• Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase

  2004cited by this paper
• Complete loss of the tumor suppressor MAD2 causes premature cyclin B degradation and mitotic failure in human somatic cells.

  2004cited by this paper
• Targets of the cyclin-dependent kinase Cdk1

  2003cited by this paper
• Identification of Phosphorylation Sites in AMP-activated Protein Kinase (AMPK) for Upstream AMPK Kinases and Study of Their Roles by Site-directed Mutagenesis*

  2003cited by this paper
• Genome-wide analysis of CREB target genes reveals a core promoter requirement for cAMP responsiveness.

  2003cited by this paper
• TSC2 mediates cellular energy response to control cell growth and survival.

  2003influential reference
• The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase.

  2003cited by this paper
• Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor

  2003cited by this paper
• Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway.

  2002cited by this paper
• Protein Engineering of Protein Kinase A Catalytic Subunits Results in the Acquisition of Novel Inhibitor Sensitivity*

  2002cited by this paper
• Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

  2002cited by this paper
• Kaposi's Sarcoma-Associated Herpesvirus K-bZIP Protein Is Phosphorylated by Cyclin-Dependent Kinases

  2001cited by this paper
• Chemical genetic approaches for the elucidation of signaling pathways.

  2001cited by this paper
• Role of AMP-activated protein kinase in mechanism of metformin action.

  2001cited by this paper
• Cell cycle regulation via p53 phosphorylation by a 5'-AMP activated protein kinase activator, 5-aminoimidazole- 4-carboxamide-1-beta-D-ribofuranoside, in a human hepatocellular carcinoma cell line.

  2001cited by this paper
• Effects of the Regulatory Light Chain Phosphorylation of Myosin II on Mitosis and Cytokinesis of Mammalian Cells*

  2000influential reference
• Chemical genetic analysis of the budding-yeast p21-activated kinase Cla4p

  2000cited by this paper
• Src-Abl tyrosine kinase chimeras: replacement of the adenine binding pocket of c-Abl with v-Src to swap nucleotide and inhibitor specificities.

  2000cited by this paper
• Incomplete cytokinesis and induction of apoptosis by overexpression of the mammalian polo-like kinase, Plk3.

  2000cited by this paper
• Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor.

  1999cited by this paper
• AMP-activated protein kinase: an ultrasensitive system for monitoring cellular energy charge.

  1999cited by this paper
• Phosphorylation of non-muscle myosin II regulatory light chain by p21-activated kinase (gamma-PAK).

  1998cited by this paper
• Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo.

  1994cited by this paper
• Insulin activation of acetyl-CoA carboxylase accompanied by inhibition of the 5'-AMP-activated protein kinase.

  1992cited by this paper
• Activation of actin-activated ATPase in smooth muscle by phosphorylation of myosin light chain with protease-activated kinase I.

  1984cited by this paper

• Ustilago maydis disrupts carbohydrate signaling networks to induce hypertrophy in host cells

  2026cites this paper
• AMPK Signaling Regulates Epithelioid Hemangioendothelioma Cell Growth

  2025cites this paper
• Energy metabolism in health and diseases

  2025cites this paper
• Small Molecule Modulators of AMP-Activated Protein Kinase (AMPK) Activity and Their Potential in Cancer Therapy

  2025cites this paper
• Novel role for Ddx39 in differentiation and telomere length regulation of embryonic stem cells

  2024cites this paper
• Protein kinase A and local signaling in cancer.

  2024cites this paper
• Nuclear localization of MTHFD2 is required for correct mitosis progression

  2024cites this paper
• How mass spectrometry can be exploited to study AMPK.

  2024cites this paper
• DCP1A, a MEK substrate, regulates the self-renewal and differentiation of mouse embryonic stem cells.

  2024cites this paper
• Therapeutic modulation of ROCK overcomes metabolic adaptation of cancer cells to OXPHOS inhibition and drives synergistic anti-tumor activity

  2024cites this paper
• An Escherichia coli-Based Phosphorylation System for Efficient Screening of Kinase Substrates

  2024cites this paper
• Regulation of Sleep Amount by CRTC1 via Transcription of Crh in Mice

  2024cites this paper
• Spatial control of the APC/C ensures the rapid degradation of cyclin B1

  2024cites this paper
• AMPK is a mechano-metabolic sensor linking cell adhesion and mitochondrial dynamics to Myosin-dependent cell migration

  2023cites this paper
• AMPK is a mechano-metabolic sensor linking cell adhesion and mitochondrial dynamics to Myosin II dependent cell migration

  2023influential citation
• Hypoxia increases persulfide and polysulfide formation by AMP kinase dependent cystathionine gamma lyase phosphorylation

  2023cites this paper
• Elevated basal AMP-activated protein kinase activity sensitizes colorectal cancer cells to growth inhibition by metformin

  2023cites this paper
• Phosphoproteomic Approaches for Identifying Phosphatase and Kinase Substrates

  2023cites this paper
• Nuclear MTHFD2 secures mitosis progression by preserving centromere integrity

  2023cites this paper
• MATR3 is an endogenous inhibitor of DUX4 in FSHD muscular dystrophy

  2023cites this paper
• TNIK is a conserved regulator of glucose and lipid metabolism in obesity

  2023cites this paper
• Inhibition of Transforming Growth Factor-β Improves Primary Renal Tubule Cell Differentiation in Long-Term Culture

  2022cites this paper
• Translation initiation factor eIF3a regulates glucose metabolism and cell proliferation via promoting small GTPase Rheb synthesis and AMPK activation

  2022cites this paper
• Unravelling the Role of Kinases That Underpin Androgen Signalling in Prostate Cancer

  2022cites this paper
• Myosin-dependent partitioning of junctional Prickle2 toward the anterior vertex during planar polarization of Xenopus neuroectoderm

  2022cites this paper
• New insights into activation and function of the AMPK

  2022cites this paper
• Phase separation-based visualization of protein-protein interactions and kinase activities in plants

  2022cites this paper
• The beta subunit of AMP-activated protein kinase is critical for cell cycle progression and parasite development in Toxoplasma gondii

  2022cites this paper
• Metformin induces mitochondrial remodeling and differentiation of pancreatic progenitor cells into beta-cells by a potential mechanism including suppression of the T1R3, PLCβ2, cytoplasmic Ca+2, and AKT

  2022cites this paper
• AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion

  2022cites this paper
• Connecting single-cell ATP dynamics to overflow metabolism, cell growth and the cell cycle in Escherichia coli

  2022cites this paper
• Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins

  2022cites this paper
• AMPK-PERK axis represses oxidative metabolism and enhances apoptotic priming of mitochondria in acute myeloid leukemia.

  2022cites this paper
• Mapping of the plant SnRK1 kinase signaling network reveals a key regulatory role for the Class II T6P synthase-like proteins

  2022cites this paper
• AMPK-mediated phosphorylation enhances the auto-inhibition of TBC1D17 to promote Rab5-dependent glucose uptake

  2021cites this paper
• Metabolic evolutionary roots of the macrophage immune response in amoeba-bacteria interactions: The conserved role of hypoxia-induced Factor and AMP kinase.

  2021cites this paper
• Defective apoptotic cell contractility provokes sterile inflammation, leading to liver damage and tumour suppression

  2021cites this paper
• Sirtuins-Mediated System-Level Regulation of Mammalian Tissues at the Interface between Metabolism and Cell Cycle: A Systematic Review

  2021cites this paper
• Metabolic syndrome and its components are associated with thyroid volume in adolescents

  2021cites this paper
• SNF1-Related Protein Kinase 1: the many-faced signaling hub regulating developmental plasticity in plants.

  2021cites this paper
• AMPKα-like proteins as LKB1 downstream targets in cell physiology and cancer

  2021cites this paper
• Proximity proteomics identifies PAK4 as a component of Afadin–Nectin junctions

  2021cites this paper
• AMPKα2 activation by an energy-independent signal ensures chromosomal stability during mitosis

  2021cites this paper
• Complementary omics strategies to dissect p53 signaling networks under nutrient stress

  2021cites this paper
• AMPK–mTOR Signaling and Cellular Adaptations in Hypoxia

  2021cites this paper
• Identification of PIM1 substrates reveals a role for NDRG1 phosphorylation in prostate cancer cellular migration and invasion

  2021cites this paper
• Purdue e-Pubs Purdue e-Pubs AMP-Activated Protein Kinase Directly Phosphorylates and AMP-Activated Protein Kinase Directly Phosphorylates and Destabilizes Hedgehog Pathway Transcription Factor GLI1 in Destabilizes Hedgehog Pathway Transcription Factor GLI1 in Medulloblastoma Medulloblastoma

  2020influential citation
• Insight on Transcriptional Regulation of the Energy Sensing AMPK and Biosynthetic mTOR Pathway Genes

  2020cites this paper
• p53-dependent elimination of aneuploid mitotic offspring by entosis

  2020cites this paper
• LKB1 inactivation leads to centromere defects and genome instability via p53-dependent upregulation of survivin

  2020cites this paper
• Effect of Edible Oil on AMP-Activated Protein Kinase Pathway in Rabbit

  2020cites this paper
• Loss of AMPKalpha1 Triggers Centrosome Amplification via PLK4 Upregulation in Mouse Embryonic Fibroblasts

  2020cites this paper
• IRSp53 is a novel interactor of SHIP2: A role of the actin binding protein Mena in their cellular localization in breast cancer cells.

  2020cites this paper
• The p21-activated kinases in neural cytoskeletal remodeling and related neurological disorders

  2020cites this paper
• Cdk1 Controls Global Epigenetic Landscape in Embryonic Stem Cells.

  2020influential citation
• Array-based Western-blotting reveals spatial differences in hepatic signaling and metabolism following CAR activation

  2020cites this paper
• Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates

  2020cites this paper
• AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy

  2020cites this paper
• Identification of PIM1 substrates reveals a role for NDRG1 in prostate cancer cellular migration and invasion

  2020cites this paper
• Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division

  2020cites this paper
• Counteracting Genome Instability by p53-dependent Mintosis

  2020cites this paper
• Quantitative proteomics indicate a strong correlation of mitotic phospho-/dephosphorylation with non-structured regions of substrates.

  2020cites this paper
• Endothelial PKA activity regulates angiogenesis by limiting autophagy through phosphorylation of ATG16L1

  2019cites this paper
• Energetic adaptations: Metabolic control of endocytic membrane traffic

  2019cites this paper
• Cyclin-dependent kinase 1-mediated AMPK phosphorylation regulates chromosome alignment and mitotic progression

  2019influential citation
• Engineering Orthogonal Polypeptide GalNAc-Transferase and UDP-Sugar Pairs.

  2019cites this paper
• Mechanistic insights into AMP-activated protein kinase-dependent gene expression

  2019cites this paper
• p90 ribosomal S6 kinase (RSK) phosphorylates myosin phosphatase and thereby controls edge dynamics during cell migration

  2019cites this paper
• Quantitative proteomics revealed a tight correlation between mitotic phosphorylation/dephosphorylation and structural properties of substrate domains

  2019cites this paper
• Endothelial PKA targets ATG16L1 to regulate angiogenesis by limiting autophagy

  2019cites this paper
• AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR

  2019cites this paper
• Illuminating the dark phosphoproteome

  2019cites this paper
• PAK2 links cell survival to mechanotransduction and metabolism

  2019cites this paper
• IRSp53 coordinates AMPK and 14-3-3 signaling to regulate filopodia dynamics and directed cell migration

  2019influential citation
• Introducing a simple model system for binding studies of known and novel inhibitors of AMPK: a therapeutic target for prostate cancer

  2019cites this paper
• AMPK-mediated activation of MCU stimulates mitochondrial Ca2+ entry to promote mitotic progression

  2019cites this paper
• AMP-activated protein kinase: the current landscape for drug development

  2019cites this paper
• Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen.

  2019cites this paper
• The significant others: Global search for direct kinase substrates using chemical approaches

  2019cites this paper
• AMPK activation increases postoperative cognitive impairment in intermittent hypoxia rats via direct activating PAK2.

  2019cites this paper
• Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks.

  2019cites this paper
• Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates.

  2019cites this paper
• AMPK regulates anaphase central spindle length by phosphorylation of KIF4A

  2018cites this paper
• Mechanism of IRSp53 inhibition by 14-3-3

  2018cites this paper
• Implication and Regulation of AMPK during Physiological and Pathological Myeloid Differentiation

  2018cites this paper
• Chitosan-assisted permeabilization of ATP-biotin for live cell kinase-catalyzed biotinylation.

  2018cites this paper
• A self‐organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide‐induced acute lung injury

  2018cites this paper
• CaMKK2 Regulates Mechanosensitive Assembly of Contractile Actin Stress Fibers.

  2018cites this paper
• Regulation of Canonical and Non-Canonical Hippo Pathway Components in Mitosis and Cancer

  2018influential citation
• Phosphorylation of CDC25C by AMP-activated protein kinase mediates a metabolic checkpoint during cell-cycle G2/M-phase transition

  2018cites this paper
• A chemical genetic approach to engineer phototropin kinases for substrate labeling

  2018cites this paper
• Snf1/AMPK is involved in the mitotic spindle alignment in Saccharomyces cerevisiae

  2018cites this paper
• Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition

  2018cites this paper
• VEGFC Antibody Therapy Drives Differentiation of AML.

  2018cites this paper
• AMPK promotes induction of a tumor suppressor FLCN through activation of TFEB independently of mTOR

  2018cites this paper
• Structure, biochemistry, and biology of PAK kinases.

  2017cites this paper
• AMPK negatively regulates tensin-dependent integrin activity

  2017cites this paper
• Kinases Involved in Both Autophagy and Mitosis

  2017cites this paper
• Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle

  2017cites this paper
• University of Dundee Multidimentional proteomics for cell biology

  2017cites this paper