The alternating-access mechanism of MFS transporters arises from inverted-topology repeats.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Journal of Molecular Biology

• Publication date

  2011-04-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.jmb.2011.02.008PMID 21315728
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The structural basis of secondary active transport mechanisms.

  2011cited by this paper
• A study of the evolution of inverted-topology repeats from LeuT-fold transporters using AlignMe.

  2010cited by this paper
• Helix dynamics in LacY: helices II and IV.

  2010cited by this paper
• Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane

  2010cited by this paper
• Quality assessment of protein model-structures using evolutionary conservation

  2010cited by this paper
• Structure of a fucose transporter in an outward-open conformation

  2010influential reference
• Structural characterization of the osmosensor ProP.

  2009cited by this paper
• Residues gating the periplasmic pathway of LacY.

  2009cited by this paper
• Probing of the rates of alternating access in LacY with Trp fluorescence

  2009cited by this paper
• Inward-facing conformation of glutamate transporters as revealed by their inverted-topology structural repeats

  2009cited by this paper
• The rocking bundle: a mechanism for ion-coupled solute flux by symmetrical transporters.

  2009cited by this paper
• Structure of a multidrug transporter

  2009cited by this paper
• Clogging the periplasmic pathway in LacY.

  2009cited by this paper
• Residues in the H+ translocation site define the pKa for sugar binding to LacY.

  2009cited by this paper
• The Cys154-->Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway.

  2008cited by this paper
• Ins and outs of major facilitator superfamily antiporters.

  2008cited by this paper
• Mechanism for alternating access in neurotransmitter transporters

  2008cited by this paper
• Opening and closing of the periplasmic gate in lactose permease

  2008cited by this paper
• Protonation and sugar binding to LacY

  2008cited by this paper
• Sugar binding in lactose permease: anomeric state of a disaccharide influences binding structure.

  2007cited by this paper
• Structural determination of wild-type lactose permease

  2007cited by this paper
• Sugar binding induces an outward facing conformation of LacY

  2007influential reference
• Single-molecule FRET reveals sugar-induced conformational dynamics in LacY

  2007cited by this paper
• Sugar transport across lactose permease probed by steered molecular dynamics.

  2007cited by this paper
• Site-directed alkylation of LacY: effect of the proton electrochemical gradient.

  2007cited by this paper
• Site-directed alkylation and the alternating access model for LacY

  2007cited by this paper
• Conformational change in an MFS protein: MD simulations of LacY.

  2007cited by this paper
• Structure of the Multidrug Transporter EmrD from Escherichia coli

  2006cited by this paper
• Using Xplor-NIH for NMR molecular structure determination

  2006influential reference
• Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences

  2006cited by this paper
• Sugar binding and protein conformational changes in lactose permease.

  2006cited by this paper
• Lessons from lactose permease.

  2006cited by this paper
• Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY

  2006cited by this paper
• Site-directed alkylation of cysteine replacements in the lactose permease of Escherichia coli: helices I, III, VI, and XI.

  2006cited by this paper
• OPM: Orientations of Proteins in Membranes database

  2006cited by this paper
• On the accuracy of homology modeling and sequence alignment methods applied to membrane proteins.

  2006cited by this paper
• Direct sugar binding to LacY measured by resonance energy transfer.

  2006cited by this paper
• Characterization of Bacterial Drug Antiporters Homologous to Mammalian Neurotransmitter Transporters

  2005cited by this paper
• Structural Genomics

  2005influential reference
• The PMDB Protein Model Database

  2005cited by this paper
• Lactose permease as a paradigm for membrane transport proteins (Review)

  2004cited by this paper
• MUSCLE: multiple sequence alignment with high accuracy and high throughput.

  2004cited by this paper
• Structure and transport mechanism of the bacterial oxalate transporter OxlT.

  2004cited by this paper
• The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors.

  2004cited by this paper
• Sugar Recognition by the Lactose Permease of Escherichia coli*

  2004cited by this paper
• Aromatic stacking in the sugar binding site of the lactose permease.

  2003cited by this paper
• GRASP2: visualization, surface properties, and electrostatics of macromolecular structures and sequences.

  2003influential reference
• Exploiting luminescence spectroscopy to elucidate the interaction between sugar and a tryptophan residue in the lactose permease of Escherichia coli

  2003cited by this paper
• Structure and Mechanism of the Glycerol-3-Phosphate Transporter from Escherichia coli

  2003cited by this paper
• The Xplor-NIH NMR molecular structure determination package.

  2003influential reference
• Using multiple structure alignments, fast model building, and energetic analysis in fold recognition and homology modeling

  2003influential reference
• Structure and Mechanism of the Lactose Permease of Escherichia coli

  2003cited by this paper
• Rate4Site: an algorithmic tool for the identification of functional regions in proteins by surface mapping of evolutionary determinants within their homologues

  2002cited by this paper
• The PyMOL Molecular Graphics System

  2002influential reference
• An approach to membrane protein structure without crystals

  2002cited by this paper
• Reweighted atomic densities to represent ensembles of NMR structures*

  2002cited by this paper
• Sequence similarity between the channel-forming domains of voltage-gated ion channel proteins and the C-terminal domains of secondary carriers of the major facilitator superfamily.

  2002cited by this paper
• Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major determinants for substrate binding.

  2001cited by this paper
• Arg-302 facilitates deprotonation of Glu-325 in the transport mechanism of the lactose permease from Escherichia coli

  2001cited by this paper
• The kamikaze approach to membrane transport

  2001cited by this paper
• Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: N-ethylmaleimide-sensitive face of helix II.

  2000cited by this paper
• Thiol cross-linking of transmembrane domains IV and V in the lactose permease of Escherichia coli.

  2000cited by this paper
• Thiol cross-linking of transmembrane domains IV and V in the lactose permease of Escherichia coli.

  2000cited by this paper
• Unraveling the mechanism of the lactose permease of Escherichia coli.

  2000cited by this paper
• TeXshade: shading and labeling of multiple sequence alignments using LaTeX2e

  2000cited by this paper
• TEXshade: shading and labeling of multiple sequence alignments using LATEX2 epsilon.

  2000cited by this paper
• Proximity between Glu126 and Arg144 in the lactose permease of Escherichia coli.

  1999cited by this paper
• The major facilitator superfamily.

  1999cited by this paper
• Asparagine and glutamine: using hydrogen atom contacts in the choice of side-chain amide orientation.

  1999cited by this paper
• Fourier transform infrared analysis of purified lactose permease: a monodisperse lactose permease preparation is stably folded, alpha-helical, and highly accessible to deuterium exchange.

  1998cited by this paper
• Major Facilitator Superfamily

  1998cited by this paper
• Fourier transform infrared spectroscopy reveals a rigid alpha-helical assembly for the tetrameric Streptomyces lividans K+ channel.

  1998cited by this paper
• All-atom empirical potential for molecular modeling and dynamics studies of proteins.

  1998cited by this paper
• The substrate-binding site in the lactose permease of Escherichia coli.

  1998cited by this paper
• Interaction between residues Glu269 (helix VIII) and His322 (helix X) of the lactose permease of Escherichia coli is essential for substrate binding.

  1997cited by this paper
• The role of helix VIII in the lactose permease of Escherichia coli: II. Site‐directed sulfhydryl modification

  1997cited by this paper
• Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

  1997cited by this paper
• Interaction between residues Glu269 (helix VIII) and His322 (helix X) of the lactose permease of Escherichia coli is essential for substrate binding.

  1997cited by this paper
• Cysteine-scanning mutagenesis of helix II and flanking hydrophilic domains in the lactose permease of Escherichia coli.

  1996cited by this paper
• Role of glutamate-269 in the lactose permease of Escherichia coli.

  1994cited by this paper
• Functional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli.

  1994cited by this paper
• Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli.

  1993cited by this paper
• Comparative protein modelling by satisfaction of spatial restraints.

  1993cited by this paper
• PROCHECK: a program to check the stereochemical quality of protein structures

  1993cited by this paper
• Thermal motions of surface alpha-helices in the D-galactose chemosensory receptor. Detection by disulfide trapping.

  1992cited by this paper
• The lactose permease of : a paradigm for membrane transport proteins

  1992cited by this paper
• The lactose permease of Escherichia coli.

  1990cited by this paper
• Basic local alignment search tool.

  1990cited by this paper
• CHARMM: A program for macromolecular energy, minimization, and dynamics calculations

  1983cited by this paper

• Conformational dynamics of the MdfA multidrug efflux pump and structural mechanisms underlying substrate efflux: A review.

  2026cites this paper
• A conserved salt bridge network stabilizes the hepatic organic anion transporters OATP1B1 and OATP1B3.

  2026cites this paper
• Multidrug resistance and major facilitator superfamily antimicrobial efflux pumps of the ESKAPEE pathogen Staphylococcus aureus

  2025cites this paper
• Quantification of Membrane Protein Conformational Free Energy from Mutations and a Single Atom

  2025cites this paper
• Identification of New Key Players for Ferrous Iron Export in the Asymmetric Inner Gate of Human Ferroportin 1

  2025cites this paper
• S292L mutation in Rv1258c efflux pump drives pyrazinamide efflux and a novel inhibitor designed for co-therapy to improve MDR-TB treatment outcomes

  2025influential citation
• Outsmarting Nightmare: Drug Resistance in Acinetobacter baumannii

  2025cites this paper
• Whole Genome Sequencing, Characterization and Comparative Genome Analysis of Acinetobacter baumannii JJAB01: A Comprehensive Insights on Antimicrobial Resistance and Virulence Genotype.

  2024cites this paper
• Energetics of substrate transport in proton-dependent oligopeptide transporters

  2024cites this paper
• The mechanism of mammalian proton-coupled peptide transporters

  2024cites this paper
• Memorization bias impacts modeling of alternative conformational states of solute carrier membrane proteins with methods from deep learning

  2024influential citation
• Major facilitator superfamily efflux pumps in human pathogens: Role in multidrug resistance and beyond

  2024cites this paper
• Energetics of substrate transport in proton-dependent oligopeptide transporters

  2024cites this paper
• Structural mechanisms for VMAT2 inhibition by tetrabenazine

  2024cites this paper
• Specificity and tunability of efflux pumps: A new role for the proton gradient?

  2024cites this paper
• Structural basis for urate recognition and apigenin inhibition of human GLUT9

  2024cites this paper
• Ins and Outs of Rocker Switch Mechanism in Major Facilitator Superfamily of Transporters

  2023cites this paper
• Structural mechanisms for VMAT2 inhibition by tetrabenazine

  2023cites this paper
• Role of bacterial efflux pumps in antibiotic resistance, virulence, and strategies to discover novel efflux pump inhibitors

  2023cites this paper
• Proton-driven alternating access in a spinster lipid transporter

  2022cites this paper
• Insights into the structure and function of the human organic anion transporter 1 in lipid bilayer membranes

  2022cites this paper
• Proton-driven alternating access in a spinster transporter, an emerging family of broad-specificity efflux pumps

  2022cites this paper
• Spontaneous Suppressors against Debilitating Transmembrane Mutants of CaMdr1 Disclose Novel Interdomain Communication via Signature Motifs of the Major Facilitator Superfamily

  2022influential citation
• Evolutionary balance between foldability and functionality of a glucose transporter

  2022cites this paper
• Novel variants provide differential stabilisation of human equilibrative nucleoside transporter 1 states

  2022cites this paper
• Structural insights into the elevator-type transport mechanism of a bacterial ZIP metal transporter

  2022cites this paper
• Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter

  2022cites this paper
• The Sugar Porter gene family of Piriformospora indica: Nomenclature, Transcript Profiling and Characterization

  2021cites this paper
• The structure of HiSiaQM defines the architecture of tripartite ATP-independent periplasmic (TRAP) transporters

  2021cites this paper
• Antimicrobial Resistance and Inorganic Nanoparticles

  2021cites this paper
• Expression, purification and characterization of human proton-coupled oligopeptide transporter 1 hPEPT1.

  2021cites this paper
• The evolutionary history of topological variations in the CPA/AT transporters

  2021cites this paper
• Evidence for a trap-and-flip mechanism in a proton-dependent lipid transporter

  2021influential citation
• A Comprehensive Review on Pharmacology of Efflux Pumps and their Inhibitors in Antibiotic Resistance.

  2021cites this paper
• Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS)

  2021cites this paper
• Successful Application of Site-directed Mutagenesis Polymerase Chain Reaction to Mutate TMS 11 of the Staphylococcal Multidrug Efflux Protein QacA

  2020cites this paper
• Mechanistic picture for chemo-mechanical couplings in a bacterial proton-coupled oligopeptide transporter from Streptococcus thermophilus

  2020cites this paper
• The evolutionary history of topological variations in the CPA/AT superfamily

  2020cites this paper
• Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.

  2020cites this paper
• Structural Basis for Blocking Sugar Uptake into the Malaria Parasite Plasmodium falciparum.

  2020cites this paper
• Structural and functional adaptability of sucrose and lactose permeases from Escherichia coli to the membrane lipid composition.

  2020cites this paper
• A CLC-ec1 mutant reveals global conformational change and suggests a unifying mechanism for the CLC Cl–/H+ transport cycle

  2020cites this paper
• Acinetobacter baumannii Efflux Pumps and Antibiotic Resistance

  2020cites this paper
• Hydrogen-Deuterium Exchange Mass-Spectrometry of Secondary Active Transporters: From Structural Dynamics to Molecular Mechanisms

  2020cites this paper
• Nonionic surfactants modulate the transport activity of ATP-binding cassette (ABC) transporters and solute carriers (SLC): Relevance to oral drug absorption.

  2019cites this paper
• Quantitative characterization of the path of glucose diffusion facilitated by human glucose transporter 1

  2019cites this paper
• Conformational Studies of Glucose Transporter 1 (GLUT1) as an Anticancer Drug Target

  2019cites this paper
• Organization of ATP-gated P2X1 receptor intracellular termini in apo and desensitized states

  2019cites this paper
• The proton electrochemical gradient induces a kinetic asymmetry in the symport cycle of LacY

  2019cites this paper
• Lactose Permease: From Membrane to Molecule to Mechanism

  2019cites this paper
• New insight into the classification and evolution of glucose transporters in the Metazoa

  2019cites this paper
• Extracellular gating of glucose transport through GLUT 1.

  2019cites this paper
• General principles of secondary active transporter function.

  2019cites this paper
• smFRET Probing Reveals Substrate-Dependent Conformational Dynamics of E. coli Multidrug MdfA.

  2019cites this paper
• Computational microscopy: Revealing molecular mechanisms in plants using molecular dynamics simulations

  2019cites this paper
• Computational Studies and Design of PPARγ and GLUT1 Inhibitors

  2019cites this paper
• The ins and outs of vesicular monoamine transporters

  2018cites this paper
• Multidrug efflux pumps: structure, function and regulation

  2018cites this paper
• Uptake dynamics in the Lactose permease (LacY) membrane protein transporter

  2018cites this paper
• Multi-drug efflux pumps : structure , function and regulation

  2018cites this paper
• EncoMPASS: an online database for analyzing structure and symmetry in membrane proteins

  2018cites this paper
• Outward open conformation of a Major Facilitator Superfamily multidrug/H+ antiporter provides insights into switching mechanism

  2018cites this paper
• Study of the relative domain stability of a two-domain E. coli MFS transporter, GlpT

  2018cites this paper
• Direct protein-lipid interactions shape the conformational landscape of secondary transporters

  2018cites this paper
• Multispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter

  2018cites this paper
• Crystal structure of an intramembranal phosphatase central to bacterial cell-wall peptidoglycan biosynthesis and lipid recycling

  2018cites this paper
• Exploration of conformational changes in lactose permease upon sugar binding and proton transfer through coarse‐grained simulations

  2017influential citation
• Symmetry and Structure in the POT Family of Proton Coupled Peptide Transporters

  2017cites this paper
• Chapter 2 Functional Properties of Organic Cation Transporter OCT 1 , Binding of Substrates and Inhibitors , and Presumed Transport Mechanism

  2017cites this paper
• Mapping Functionally Important Residues in the Na+/Dicarboxylate Cotransporter, NaDC1.

  2017cites this paper
• Two Cl Ions and a Glu Compete for a Helix Cage in the CLC Proton/Cl- Antiporter.

  2017cites this paper
• Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling.

  2017cites this paper
• Asymmetry of inverted-topology repeats in the AE1 anion exchanger suggests an elevator-like mechanism

  2017cites this paper
• Mycobacterium tuberculosis Major Facilitator Superfamily Transporters

  2017cites this paper
• Ligand Discovery for the Nutrient Transporters ASCT2 and LAT‐1 from Homology Modeling and Virtual Screening

  2017cites this paper
• Functional Roles of Highly Conserved Amino Acid Sequence Motifs A and C in Solute Transporters of the Major Facilitator Superfamily

  2017cites this paper
• Chloride and Proton Binding in the E. coli 2Cl¯:1H+ CLC Exchanger

  2017cites this paper
• Nature et conséquences des interactions entre transporteurs membranaires et pesticides

  2017cites this paper
• Drug Resistance in Bacteria , Fungi , Malaria , and Cancer

  2017cites this paper
• Applications of sequence coevolution in membrane protein biochemistry.

  2017cites this paper
• Substrate transport pathway inside outward open conformation of EmrD: a molecular dynamics simulation study.

  2016cites this paper
• Bacterial Resistance to Host Defence Peptides

  2016cites this paper
• Functional Properties of Organic Cation Transporter OCT1, Binding of Substrates and Inhibitors, and Presumed Transport Mechanism

  2016cites this paper
• The bacterial dicarboxylate transporter, VcINDY, uses a two-domain elevator-type mechanism

  2016cites this paper
• Shared Molecular Mechanisms of Membrane Transporters.

  2016cites this paper
• Inward open characterization of EmrD transporter with molecular dynamics simulation.

  2016cites this paper
• A Numbering System for MFS Transporter Proteins

  2016influential citation
• Structure and mechanism of the ATP synthase membrane motor inferred from quantitative integrative modeling

  2016cites this paper
• Helical unwinding and side-chain unlocking unravel the outward open conformation of the melibiose transporter

  2016cites this paper
• Accurate Prediction of Ligand Affinities for a Proton-Dependent Oligopeptide Transporter

  2016cites this paper
• Modeling structural transitions from the periplasmic-open state of lactose permease and interpretations of spin label experiments.

  2016cites this paper
• SLC Transporters: Structure, Function, and Drug Discovery.

  2016cites this paper
• Inhibitor Discovery for the Human GLUT1 from Homology Modeling and Virtual Screening.

  2016cites this paper
• The V-motifs facilitate the substrate capturing step of the PTS elevator mechanism.

  2016cites this paper
• Functional characterization of iron transporters Nramp 1 and Nramp 2 from Dictyostelium discoideum : a model of cellular iron homeostasis

  2016cites this paper
• Protonation of Glu(135) Facilitates the Outward-to-Inward Structural Transition of Fucose Transporter.

  2015cites this paper
• Structural comparison of bacterial multidrug efflux pumps of the major facilitator superfamily.

  2015cites this paper
• Crystal Structures of the E xtracellular Domain from PepT 1 and PepT 2 Provide Novel Insights into Mammalian Peptide Transport Graphical

  2015cites this paper
• Structure alignment of membrane proteins: Accuracy of available tools and a consensus strategy

  2015cites this paper
• Structure-Based Identification of Inhibitors for the SLC13 Family of Na(+)/Dicarboxylate Cotransporters.

  2015cites this paper