Use of Imaging Techniques to Illuminate Dynamics of Hematopoietic Stem Cells and Their Niches

Continuous generation of blood cells over an organism's lifetime is supported by hematopoietic stem/progenitor cells (HSPCs) capable of producing all hematopoietic cell subtypes. Adult mammalian HSPCs are localized to bone marrow and regulated by their neighboring microenvironment, or “niche.” Because interactions of HSPCs with their niches are highly dynamic and complex, the recent development of imaging technologies provides a powerful new tool to understand stem cell/niche biology. In this review, we discuss recent advances in our understanding of dynamic HSPC/niche interactions during development, homeostasis, disease states or aging with a focus on studies advanced by imaging analysis. We also summarize methods to visualize HSPCs and niche cells in vivo, including use of HSPC reporter mice and chemical probes. Findings emerging from these investigations could suggest novel therapies for diseases and aging.

• Publication year

  2017

• Venue

  Front. Cell Dev. Biol.

• Publication date

  2017-06-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3389/fcell.2017.00062PMID 28660186PMCID 5468376
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2015influential reference
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• Whole-body imaging with single-cell resolution by tissue decolorization.

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• Arteriolar niches maintain haematopoietic stem cell quiescence

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• Infection-responsive expansion of the hematopoietic stem and progenitor cell compartment in zebrafish is dependent upon inducible nitric oxide.

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• Inflammatory modulation of HSCs: viewing the HSC as a foundation for the immune response

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• Regulation of the HIF-1alpha level is essential for hematopoietic stem cells.

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• Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche

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• Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair.

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• Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells

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• In vivo cellular imaging pinpoints the role of reactive oxygen species in the early steps of adult hematopoietic reconstitution.

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• Transparent adult zebrafish as a tool for in vivo transplantation analysis.

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• In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment

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• Haematopoietic stem cells retain long-term repopulating activity and multipotency in the absence of stem-cell leukaemia SCL/tal-1 gene

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  1995cited by this paper
• Absence of yolk sac hematopoiesis from mice with a targeted disruption of the scl gene.

  1995cited by this paper
• Determination of lymphocyte division by flow cytometry.

  1994cited by this paper
• Differentiation and proliferation of hematopoietic stem cells.

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• New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy.

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