Nonhomologous DNA End Joining in Cell-Free Extracts

Among various DNA damages, double-strand breaks (DSBs) are considered as most deleterious, as they may lead to chromosomal rearrangements and cancer when unrepaired. Nonhomologous DNA end joining (NHEJ) is one of the major DSB repair pathways in higher organisms. A large number of studies on NHEJ are based on in vitro systems using cell-free extracts. In this paper, we summarize the studies on NHEJ performed by various groups in different cell-free repair systems.

• Publication year

  2010

• Venue

  Journal of Nucleic Acids

• Publication date

  2010-09-22

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4061/2010/389129PMID 20936167PMCID 2945661
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Efficiency of nonhomologous DNA end joining varies among somatic tissues, despite similarity in mechanism

  2011cited by this paper
• Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70

  2010cited by this paper
• Alternative end-joining is suppressed by the canonical NHEJ component Xrcc4/ligase IV during chromosomal translocation formation

  2010cited by this paper
• The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway.

  2010cited by this paper
• Roles for NBS1 in alternative nonhomologous end-joining of V(D)J recombination intermediates.

  2009cited by this paper
• Multiple functions of MRN in end-joining pathways during isotype class switching

  2009cited by this paper
• Mechanisms of double-strand break repair in somatic mammalian cells.

  2009cited by this paper
• Parp1 facilitates alternative NHEJ, whereas Parp2 suppresses IgH/c-myc translocations during immunoglobulin class switch recombination

  2009cited by this paper
• Coordinate 5′ and 3′ endonucleolytic trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase

  2008cited by this paper
• Flexibility in the order of action and in the enzymology of the nuclease, polymerases, and ligase of vertebrate non-homologous DNA end joining: relevance to cancer, aging, and the immune system

  2008cited by this paper
• The endless tale of non-homologous end-joining

  2008cited by this paper
• Up-regulation of WRN and DNA ligase IIIalpha in chronic myeloid leukemia: consequences for the repair of DNA double-strand breaks.

  2008cited by this paper
• MMEJ repair of double-strand breaks (director's cut): deleted sequences and alternative endings.

  2008cited by this paper
• Non-homologous end-joining for repairing I-SceI-induced DNA double strand breaks in human cells.

  2007cited by this paper
• Defects in XRCC4 and KU80 differentially affect the joining of distal nonhomologous ends

  2007cited by this paper
• IgH class switching and translocations use a robust non-classical end-joining pathway

  2007cited by this paper
• Rag mutations reveal robust alternative end joining

  2007cited by this paper
• DNA repair after irradiation in glioma cells and normal human astrocytes.

  2007cited by this paper
• XRCC4:DNA ligase IV can ligate incompatible DNA ends and can ligate across gaps

  2007cited by this paper
• Preventing or reducing late side effects of radiation therapy: radiobiology meets molecular pathology

  2006cited by this paper
• Distinct Mechanisms of Nonhomologous End Joining in the Repair of Site-Directed Chromosomal Breaks with Noncomplementary and Complementary Ends

  2006cited by this paper
• DNA end joining activity is reduced in Alzheimer's disease.

  2006cited by this paper
• Roles of nonhomologous DNA end joining, V(D)J recombination, and class switch recombination in chromosomal translocations.

  2006cited by this paper
• DNA double strand break repair in brain: reduced NHEJ activity in aging rat neurons.

  2006cited by this paper
• XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining.

  2006cited by this paper
• Cernunnos, a novel nonhomologous end-joining factor, is mutated in human immunodeficiency with microcephaly.

  2006cited by this paper
• DNA double-strand break repair: all's well that ends well.

  2006cited by this paper
• Efficient repair of bleomycin-induced double-strand breaks in barley ribosomal genes.

  2006cited by this paper
• Assessment of tumor characteristic gene expression in cell lines using a tissue similarity index (TSI).

  2005cited by this paper
• Processing of DNA for nonhomologous end‐joining by cell‐free extract

  2005influential reference
• The Artemis:DNA-PKcs endonuclease cleaves DNA loops, flaps, and gaps.

  2005cited by this paper
• Mechanism of DNA double-strand break repair by non-homologous end joining.

  2005cited by this paper
• A biochemically defined system for mammalian nonhomologous DNA end joining.

  2004cited by this paper
• Nonhomologous end joining of complementary and noncomplementary DNA termini in mouse testicular extracts.

  2004influential reference
• Molecular views of recombination proteins and their control

  2003cited by this paper
• Development of a rapid, small-scale DNA repair assay for use on clinical samples.

  2003influential reference
• Mechanism and regulation of human non-homologous DNA end-joining

  2003cited by this paper
• Repair of Sequence-specific 125I-induced Double-strand Breaks by Nonhomologous DNA End Joining in Mammalian Cell-free Extracts*

  2002cited by this paper
• Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair.

  2002cited by this paper
• Association of DNA Polymerase μ (pol μ) with Ku and Ligase IV: Role for pol μ in End-Joining Double-Strand Break Repair

  2002cited by this paper
• Leaky Scid phenotype associated with defective V(D)J coding end processing in Artemis-deficient mice.

  2002cited by this paper
• Unrepaired DNA breaks in p53-deficient cells lead to oncogenic gene amplification subsequent to translocations.

  2002cited by this paper
• A Physical and Functional Interaction between Yeast Pol4 and Dnl4-Lif1 Links DNA Synthesis and Ligation in Nonhomologous End Joining*

  2002cited by this paper
• Absence of major defects in non-homologous DNA end joining in human breast cancer cell lines

  2002cited by this paper
• The embryonic lethality in DNA ligase IV-deficient mice is rescued by deletion of Ku: implications for unifying the heterogeneous phenotypes of NHEJ mutants.

  2002cited by this paper
• Sensing and repairing DNA double-strand breaks.

  2002cited by this paper
• Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.

  2002cited by this paper
• Non‐homologous DNA end joining in the mature rat brain

  2002cited by this paper
• Genetic Recombination

  2002cited by this paper
• Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency.

  2001cited by this paper
• Nonhomologous DNA end joining in cell-free systems.

  2001cited by this paper
• DNA double strand break repair and chromosomal translocation: Lessons from animal models

  2001cited by this paper
• In vitro repair of complex unligatable oxidatively induced DNA double-strand breaks by human cell extracts.

  2001cited by this paper
• Comparison of DNA repair protein expression and activities between human fibroblast cell lines with different radiosensitivities

  2000cited by this paper
• DNA ligase IV deficiency in mice leads to defective neurogenesis and embryonic lethality via the p53 pathway.

  2000cited by this paper
• Mouse testicular extracts process DNA double-strand breaks efficiently by DNA end-to-end joining.

  1999cited by this paper
• The role of homologous recombination processes in the repair of severe forms of DNA damage in mammalian cells.

  1999cited by this paper
• Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.

  1998cited by this paper
• DNA end-joining catalyzed by human cell-free extracts.

  1998influential reference
• Productive and Nonproductive Complexes of Ku and DNA-Dependent Protein Kinase at DNA Termini

  1998cited by this paper
• DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes.

  1998cited by this paper
• Rad51‐deficient vertebrate cells accumulate chromosomal breaks prior to cell death

  1998cited by this paper
• Homologous recombination and non‐homologous end‐joining pathways of DNA double‐strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells

  1998cited by this paper
• Yeast DNA ligase IV mediates non-homologous DNA end joining

  1997cited by this paper
• Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV.

  1997cited by this paper
• DNA-PK: at the cross-roads of biochemistry and genetics.

  1997cited by this paper
• DNA repair activity in protein extracts from rat tissues

  1997cited by this paper
• Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells

  1997cited by this paper
• Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination.

  1997cited by this paper
• Mechanisms of nonhomologous DNA end-joining in frogs, mice and men.

  1996cited by this paper
• DNA Ligase IV from HeLa Cell Nuclei*

  1996cited by this paper
• The joining of non-complementary DNA double-strand breaks by mammalian extracts.

  1996cited by this paper
• Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates.

  1996cited by this paper
• The DNA-dependent Protein Kinase Is Inactivated by Autophosphorylation of the Catalytic Subunit (*)

  1996influential reference
• Nomenclature of human genes involved in ionizing radiation sensitivity.

  1995cited by this paper
• Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line

  1995influential reference
• DNA end-joining in extracts from human cells.

  1995influential reference
• Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.

  1995cited by this paper
• Nonhomologous recombination in human cells

  1994cited by this paper
• Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks.

  1994cited by this paper
• Characterization of DNA end joining in a mammalian cell nuclear extract: junction formation is accompanied by nucleotide loss, which is limited and uniform but not site specific

  1994cited by this paper
• Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA.

  1994cited by this paper
• Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination.

  1994cited by this paper
• EBP-80, a transcription factor closely resembling the human autoantigen Ku, recognizes single- to double-strand transitions in DNA.

  1993cited by this paper
• Homologous and illegitimate recombination in developing Xenopus oocytes and eggs

  1993cited by this paper
• Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: characterization by electron microscopy.

  1993cited by this paper
• A mechanism for deletion formation in DNA by human cell extracts: the involvement of short sequence repeats.

  1992cited by this paper
• Genetic analysis of ionising radiation sensitive mutants of cultured mammalian cell lines.

  1991cited by this paper
• The genetic basis of resistance to ionising radiation damage in cultured mammalian cells.

  1991cited by this paper
• A novel pathway of DNA end-to-end joining.

  1990cited by this paper
• The rejoining of double-strand breaks in DNA by human cell extracts.

  1990cited by this paper
• Joining of nonhomologous DNA double strand breaks in vitro.

  1988cited by this paper
• Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts.

  1988cited by this paper
• Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction

  1986influential reference
• Mechanism of interaction between Ku protein and DNA.

  1986cited by this paper
• Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.

  1986cited by this paper
• Use of monoclonal antibodies for the characterization of novel DNA- binding proteins recognized by human autoimmune sera

  1985cited by this paper
• Mechanisms of nonhomologous recombination in mammalian cells

  1985influential reference
• Relative rates of homologous and nonhomologous recombination in transfected DNA.

  1985cited by this paper
• Double‐stranded DNA induces the phosphorylation of several proteins including the 90 000 mol. wt. heat‐shock protein in animal cell extracts.

  1985cited by this paper

• Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations

  2025cites this paper
• Tissue-Specific Effects of Aging on Repeat-Mediated Mutation Hotspots In Vivo

  2024cites this paper
• CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination

  2023cites this paper
• A rapid multiplex cell-free assay on biochip to evaluate functional aspects of double-strand break repair

  2022cites this paper
• 20 years of DNA Polymerase μ, the polymerase that still surprises

  2021cites this paper
• Impact of Preanalytical and Analytical Methods on Cell-Free DNA Diagnostics

  2021cites this paper
• Nonhomologous end joining: new accessory factors fine tune the machinery.

  2021cites this paper
• Melatonin: A smart molecule in the DNA repair system

  2021cites this paper
• Molecular Carcinogenesis

  2020cites this paper
• Identification and characterization of novel SCR7‐based small‐molecule inhibitor of DNA end‐joining, SCR130 and its relevance in cancer therapeutics

  2020cites this paper
• In Vitro Detection of Long Noncoding RNA Generated from DNA Double-Strand Breaks.

  2019cites this paper
• DNA double-strand break repair in Penaeus monodon is predominantly dependent on homologous recombination

  2017cites this paper
• Acquired Chromosomal Abnormalities and Their Potential Formation Mechanisms in Solid Tumours

  2017cites this paper
• Homologous recombination-mediated repair of DNA double-strand breaks operates in mammalian mitochondria

  2017cites this paper
• FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4.

  2016cites this paper
• Induction of DNA damage and erroneous repair can explain genomic instability caused by endosulfan.

  2016cites this paper
• Homology and enzymatic requirements of microhomology-dependent alternative end joining

  2015cites this paper
• DNA double-strand break repair inhibitors as cancer therapeutics.

  2015cites this paper
• Individual capacity for detoxification of genotoxic compounds and repair of DNA damage. Commonly used methods for assessment of capacity for DNA repair

  2014cites this paper
• DNA repair and chromatin DNA-Reparatur und Chromatin Vom Fachbereich Biologie der Technischen Universität Darmstadt zur Erlangung des akademischen Grades eines Doctor rerum naturalium genehmigte Dissertation von Diplom-Biologin

  2014cites this paper
• Inhibition of Both EGFR and IGF1R Sensitized Prostate Cancer Cells to Radiation by Synergistic Suppression of DNA Homologous Recombination Repair

  2013cites this paper
• Role of recombination activating genes in the generation of antigen receptor diversity and beyond

  2012cites this paper
• An inhibitor of nonhomologous end-joining abrogates double-strand break repair and impedes cancer progression.

  2012cites this paper
• Spatial and temporal organization of multi-protein assemblies: achieving sensitive control in information-rich cell-regulatory systems

  2012cites this paper
• A dual role for UVRAG in maintaining chromosomal stability independent of autophagy.

  2012cites this paper
• Time-dependent predominance of nonhomologous DNA end-joining pathways during embryonic development in mice.

  2012cites this paper
• How does DNA break during chromosomal translocations?

  2011cites this paper
• Modeling non-homologous end joining.

  2011cites this paper