Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury

Besides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also uncovered novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures. Our data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.

• Publication year

  2019

• Venue

  eLife

• Publication date

  2019-03-26

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.7554/eLife.43882PMID 30912746PMCID 6459677
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Sequence Analysis

  2020cited by this paper
• On Transcription

  2020influential reference
• Endothelial to Mesenchymal Transition in Cardiovascular Disease: JACC State-of-the-Art Review.

  2019cited by this paper
• ggplot2

  2019cited by this paper
• Macrophage Biology, Classification, and Phenotype in Cardiovascular Disease: JACC Macrophage in CVD Series (Part 1).

  2018cited by this paper
• Single-Cell Transcriptome Analyses Reveal Endothelial Cell Heterogeneity in Tumors and Changes following Antiangiogenic Treatment.

  2018cited by this paper
• The Macrophage in Cardiac Homeostasis and Disease: JACC Macrophage in CVD Series

  2018influential reference
• Meox1 accelerates myocardial hypertrophic decompensation through Gata4

  2018cited by this paper
• Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart.

  2018cited by this paper
• Erythro-myeloid progenitors contribute endothelial cells to blood vessels

  2018cited by this paper
• The Macrophage in Cardiac Homeostasis and Disease: JACC Macrophage in CVD Series (Part 4).

  2018influential reference
• Self-renewing resident cardiac macrophages limit adverse remodeling following myocardial infarction

  2018cited by this paper
• Resident fibroblast expansion during cardiac growth and remodeling.

  2018cited by this paper
• PDGFRα+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo

  2018cited by this paper
• Regulation of Cell Cycle to Stimulate Adult Cardiomyocyte Proliferation and Cardiac Regeneration.

  2018cited by this paper
• WNT Signaling in Cardiac and Vascular Disease

  2018cited by this paper
• Multi-ethnic genome-wide association study for atrial fibrillation

  2018cited by this paper
• Integrating single-cell transcriptomic data across different conditions, technologies, and species

  2018cited by this paper
• Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart

  2018cited by this paper
• The Hippo pathway in the heart: pivotal roles in development, disease, and regeneration

  2018cited by this paper
• Single-Cell Sequencing of the Healthy and Diseased Heart Reveals Cytoskeleton-Associated Protein 4 as a New Modulator of Fibroblasts Activation

  2018cited by this paper
• The Human Heart Contains Distinct Macrophage Subsets with Divergent Origins and Functions

  2018cited by this paper
• Cardioimmunology: the immune system in cardiac homeostasis and disease

  2018influential reference
• Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts

  2018cited by this paper
• Multicellular Transcriptional Analysis of Mammalian Heart Regeneration

  2017cited by this paper
• Frequency of mononuclear diploid cardiomyocytes underlies natural variation in heart regeneration

  2017cited by this paper
• The cardiac microenvironment uses non‐canonical WNT signaling to activate monocytes after myocardial infarction

  2017influential reference
• Loss of Macrophage Wnt Secretion Improves Remodeling and Function After Myocardial Infarction in Mice

  2017cited by this paper
• Neurovascular patterning cues and implications for central and peripheral neurological disease

  2017cited by this paper
• Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

  2017cited by this paper
• IRF3 and Type I Interferons Fuel a Fatal Response to Myocardial Infarction

  2017cited by this paper
• Iterative random forests to discover predictive and stable high-order interactions

  2017cited by this paper
• On the value of therapeutic interventions targeting the complement system in acute myocardial infarction.

  2017cited by this paper
• C1q: A fresh look upon an old molecule.

  2017cited by this paper
• Macrophages Facilitate Electrical Conduction in the Heart.

  2017cited by this paper
• IL-11 is a crucial determinant of cardiovascular fibrosis

  2017influential reference
• Macrophages and fibroblasts during inflammation and tissue repair in models of organ regeneration

  2017cited by this paper
• Scaling single-cell genomics from phenomenology to mechanism

  2017cited by this paper
• Fibromodulin reduces scar formation in adult cutaneous wounds by eliciting a fetal-like phenotype

  2017influential reference
• PDGFRα signaling in cardiac fibroblasts modulates quiescence, metabolism and self-renewal, and promotes anatomical and functional repair

  2017cited by this paper
• ggplot2 - Elegant Graphics for Data Analysis (2nd Edition)

  2017cited by this paper
• Preexisting endothelial cells mediate cardiac neovascularization after injury

  2017cited by this paper
• Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration

  2017cited by this paper
• Redefining the identity of cardiac fibroblasts

  2017cited by this paper
• Novel therapeutic strategies targeting fibroblasts and fibrosis in heart disease

  2016cited by this paper
• PANTHER version 11: expanded annotation data from Gene Ontology and Reactome pathways, and data analysis tool enhancements

  2016cited by this paper
• Primitive Embryonic Macrophages are Required for Coronary Development and Maturation.

  2016influential reference
• In Vivo Cellular Reprogramming: The Next Generation.

  2016cited by this paper
• Cardiac Fibrosis: The Fibroblast Awakens.

  2016influential reference
• The STRING database in 2017: quality-controlled protein–protein association networks, made broadly accessible

  2016cited by this paper
• Matricellular Protein CCN5 Reverses Established Cardiac Fibrosis.

  2016cited by this paper
• Massively parallel digital transcriptional profiling of single cells

  2016cited by this paper
• Defining the Cardiac Fibroblast.

  2016cited by this paper
• The transcription factor scleraxis is a critical regulator of cardiac fibroblast phenotype

  2016cited by this paper
• Abandoning M1/M2 for a Network Model of Macrophage Function.

  2016cited by this paper
• Revisiting Cardiac Cellular Composition.

  2016cited by this paper
• Genetic lineage tracing defines myofibroblast origin and function in the injured heart

  2016influential reference
• Self-renewing resident arterial macrophages arise from embryonic CX3CR1+ precursors and circulating monocytes immediately after birth

  2015cited by this paper
• Loss of Secreted Frizzled-Related Protein-1 Leads to Deterioration of Cardiac Function in Mice and Plays a Role in Human Cardiomyopathy

  2015cited by this paper
• destiny: diffusion maps for large-scale single-cell data in R

  2015cited by this paper
• Novel Markers to Delineate Murine M1 and M2 Macrophages

  2015cited by this paper
• PDGFRα demarcates the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium

  2015cited by this paper
• Role of innate and adaptive immune mechanisms in cardiac injury and repair

  2015influential reference
• A draft network of ligand–receptor-mediated multicellular signalling in human

  2015cited by this paper
• Tumor necrosis factor: a mechanistic link between angiotensin-II-induced cardiac inflammation and fibrosis.

  2015cited by this paper
• ERBB2 triggers mammalian heart regeneration by promoting cardiomyocyte dedifferentiation and proliferation

  2015cited by this paper
• Nerves Regulate Cardiomyocyte Proliferation and Heart Regeneration.

  2015cited by this paper
• Mesenchymal cell contributions to the stem cell niche.

  2015cited by this paper
• Monocyte and macrophage plasticity in tissue repair and regeneration.

  2015cited by this paper
• Macrophages are required for neonatal heart regeneration.

  2014cited by this paper
• WIF1 can effectively co-regulate pro-apoptotic activity through the combination with DKK1.

  2014cited by this paper
• Fibroblasts in myocardial infarction: a role in inflammation and repair.

  2014cited by this paper
• Distinct macrophage lineages contribute to disparate patterns of cardiac recovery and remodeling in the neonatal and adult heart

  2014influential reference
• Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis.

  2014cited by this paper
• LocTree3 prediction of localization

  2014cited by this paper
• Embryonic and adult-derived resident cardiac macrophages are maintained through distinct mechanisms at steady state and during inflammation.

  2014cited by this paper
• Progressive replacement of embryo-derived cardiac macrophages with age

  2014cited by this paper
• Differential Contribution of Monocytes to Heart Macrophages in Steady-State and After Myocardial Infarction

  2014cited by this paper
• Fibromodulin-Deficiency Alters Temporospatial Expression Patterns of Transforming Growth Factor-β Ligands and Receptors during Adult Mouse Skin Wound Healing

  2014cited by this paper
• R: A language and environment for statistical computing.

  2014cited by this paper
• Mesenchymal-endothelial-transition contributes to cardiac neovascularization

  2014influential reference
• Connective tissue cells, but not muscle cells, are involved in establishing the proximo-distal outcome of limb regeneration in the axolotl

  2013cited by this paper
• Circulating and Tissue Resident Endothelial Progenitor Cells

  2013cited by this paper
• WIF1 causes dysfunction of heart in transgenic mice

  2013cited by this paper
• STAR: ultrafast universal RNA-seq aligner

  2013cited by this paper
• Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells

  2013cited by this paper
• Interferon Induced IFIT Family Genes in Host Antiviral Defense

  2013cited by this paper
• featureCounts: an efficient general purpose program for assigning sequence reads to genomic features

  2013cited by this paper
• The Wnt antagonist Wif‐1 interacts with CTGF and inhibits CTGF activity

  2012cited by this paper
• Data exploration, quality control and testing in single-cell qPCR-based gene expression experiments

  2012cited by this paper
• Thrombospondin‐4 regulates fibrosis and remodeling of the myocardium in response to pressure overload

  2012cited by this paper
• Fast gapped-read alignment with Bowtie 2

  2012cited by this paper
• Characterization of a Wnt‐binding site of the WIF‐domain of Wnt inhibitory factor‐1

  2012cited by this paper
• Adult cardiac-resident MSC-like stem cells with a proepicardial origin.

  2011cited by this paper
• Adult mouse epicardium modulates myocardial injury by secreting paracrine factors.

  2011cited by this paper
• Revealing New Mouse Epicardial Cell Markers through Transcriptomics

  2010cited by this paper
• Neurovascular congruence during cerebral cortical development.

  2009cited by this paper
• Secreted frizzled related protein 2 (Sfrp2) is the key Akt-mesenchymal stem cell-released paracrine factor mediating myocardial survival and repair

  2007cited by this paper
• The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions

  2007cited by this paper
• On the value of IT

  2005cited by this paper

• Extracellular BRICK1 drives heart repair after myocardial infarction in mice.

  2026cites this paper
• Agrin at the Crossroads of Aging: A Pleiotropic Regulator in Age-Related Diseases.

  2026cites this paper
• Endothelial-to-Mesenchymal Transition in Post-Myocardial Infarction Fibrosis: A Maladaptive but Targetable Pathway

  2026cites this paper
• Neurovascular interactions in the ageing heart.

  2026cites this paper
• Psychological stress and myocardial extracellular matrix remodeling- a pathologic synergy.

  2026cites this paper
• Cardio-Vascular Extracellular Matrix: The Unmet Enigma

  2026cites this paper
• Dapagliflozin mitigates myocardial inflammation and metabolic stress in heart failure through STAT1 inhibition: Evidence from multi-omics analyses and experimental exploration.

  2026cites this paper
• The emerging role of interleukin-41: a novel biomarker and therapeutic target in inflammation and immune-related diseases.

  2026cites this paper
• Mouse digit AAV gene delivery into fibroblasts regulates regenerative outcome

  2026cites this paper
• In vitro polarized macrophages ameliorate adverse cardiac remodeling in a mouse model of transverse aortic constriction

  2026cites this paper
• Activation of developmental transcription factors using RNA technology promotes heart repair

  2026cites this paper
• Unraveling Cardiovascular Development and Function: Insights From Single-Cell Omics.

  2026cites this paper
• Single-cell multi-omics for precision cardiovascular and longevity medicine: from methods to clinical translation

  2025influential citation
• Mapping the knowledge of omics in myocardial infarction: A scientometric analysis in R Studio, VOSviewer, Citespace, and SciMAT

  2025cites this paper
• Targeting the Notch signaling pathway: molecular mechanisms and therapeutic strategies for cardiac repair after myocardial infarction

  2025cites this paper
• Sacubitril/Valsartan attenuates progression of diabetic cardiomyopathy through immunomodulation properties: an opportunity to prevent progressive disease

  2025cites this paper
• Temporal regulation of genetic programs governing multiple cell death during myocardial ischemia-reperfusion injury

  2025cites this paper
• Brain macrophages and pial fibroblasts promote inflammation in a hypomyelination model

  2025cites this paper
• Cardiac macrophage: Insights from murine models to translational potential for human studies.

  2025cites this paper
• Shexiang baoxin pill ameliorates cardiac fibrosis by inhibiting fibroblast to myofibroblast transition through STAT3 phosphorylation suppression.

  2025cites this paper
• Single-Cell Multi-Omics Identifies Specialized Cytotoxic and Migratory CD8+ Effector T Cells in Acute Myocarditis.

  2025cites this paper
• Cardiac lymphatics retain LYVE-1-dependent macrophages during neonatal mouse heart regeneration

  2025influential citation
• Activated fatty acid synthesis pathway in macrophages propagates pathogenic fibroblast expansion after myocardial infarction

  2025cites this paper
• Rewiring the scar: translational advances in cardiac fibrosis

  2025cites this paper
• Cardiac Regeneration and Repair in Zebrafish and Mammalian Models

  2025cites this paper
• Pathogenesis and treatment strategies of sepsis-induced myocardial injury: modern and traditional medical perspectives

  2025cites this paper
• A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts

  2025cites this paper
• Podoplanin-positive cell-derived small extracellular vesicles contribute to cardiac amyloidosis after myocardial infarction

  2025cites this paper
• Cardiac fibroblasts counterbalance cardiomyocytes in LMNA cardiomyopathy pathogenesis

  2025cites this paper
• Macrophage ITGAV is dispensable for post-infarction remodeling in mice and does not mediate fibronectin responses

  2025cites this paper
• The Application of scRNA‐Seq in Heart Development and Regeneration

  2025cites this paper
• Quasi-spatial single-cell transcriptome based on physical tissue properties defines early aging associated niche in liver

  2025cites this paper
• Fibroblasts-The emerging therapeutic target of the cardiovascular system?

  2025cites this paper
• MIF-ACKR3 causes irreversible fat loss by impairing adipogenesis in cancer cachexia.

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• CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction

  2025cites this paper
• The integration of scRNA-seq with microarray and in-vivo experiments facilitates a comprehensive elucidation of the molecular mechanisms underlying endothelial cell involvement in myocardial infarction.

  2025cites this paper
• AI-based Predictive Signaling Pathway Profiling in Cardiac Fibrosis Suggests a Novel Combinatorial Treatment Strategy

  2025cites this paper
• Dynamic molecular atlas of cardiac fibrosis at single-cell resolution shows CD248 in cardiac fibroblasts orchestrates interactions with immune cells

  2025cites this paper
• Macrophages of the Heart: Homeostasis and Disease.

  2025cites this paper
• Post-myocardial Infarction Cardiac Remodeling: Multidimensional Mechanisms and Clinical Prospects of Stem Cell Therapy

  2025cites this paper
• SLAMF7 Restrains Pro-Inflammatory Macrophage Activation to Counteract Doxorubicin-Induced Cardiotoxicity

  2025cites this paper
• The role of non-coding RNAs derived from macrophage exosomes in cardiovascular diseases.

  2025cites this paper
• Single-Cell RNA Sequencing Identifies a Unique ZEB2-Regulated Clec4d+ Macrophage State Contributing to Early Inflammatory Injury in Cardiac Hypertrophy.

  2025cites this paper
• Temporal dynamics of the multi-omic response reveals the modulation of macrophage subsets post-myocardial infarction

  2025cites this paper
• Cardiac mural cells are rate-limiting for coronary vascularization after heart injury

  2025cites this paper
• Heterogeneity and characteristic changes in cardiomyocytes and non-cardiomyocytes following myocardial infarction: Insights from single-cell sequencing analysis (Review)

  2025influential citation
• Cardiac fibroblasts in myocardial injury and heart failure.

  2025cites this paper
• Sirtuin-Mediated Modulation of Cardiac Fibrosis: Emerging Molecular Insights and Therapeutic Perspectives.

  2025cites this paper
• Cardiac macrophages and their functions in homeostasis and injury.

  2025cites this paper
• Inhibition of Scarb1 on Endothelial Cells Attenuates Pressure Overload-Induced Heart Failure Progression

  2025cites this paper
• Spatial multiomics of acute myocardial infarction reveals immune cell infiltration through the endocardium

  2025cites this paper
• The Role of Cardiac Fibroblast Heterogeneity in Myocardial Fibrosis and Its Novel Therapeutic Potential

  2025cites this paper
• Omics-based Approach Towards Macrophages: New Perspectives of Biology and Function in the Normal and Diseased Heart

  2025cites this paper
• Single-cell epigenomic and transcriptomic analysis unveils the pivotal role of GATA5/ISL1+ fibroblasts in cardiac repair post-myocardial infarction.

  2025cites this paper
• Selective inhibition of stromal mechanosensing suppresses cardiac fibrosis

  2025cites this paper
• From cancer to heart fibrosis - GLIPR1 highlights a subset of myofibroblasts responsive to mesenchymal stem cell therapy after myocardial infarction.

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• Location and aetiology are determinants of fibroblast activation and heterogeneity in the failing human heart

  2025cites this paper
• Uncovering Hippo pathway-related biomarkers in acute myocardial infarction via scRNA-seq binding transcriptomics

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• Single-cell RNA sequencing analysis identified GARP-expressing myeloid cells that deteriorate post-infarct myocardial remodeling.

  2025cites this paper
• Fibroblast-Specific Loss of TGF-β Signaling Mediates Lipomatous Metaplasia in the Infarcted Heart.

  2025cites this paper
• Temporal mineralocorticoid receptor activation regulates the molecular clock and transcription of cardiovascular disease modulators in myeloid cells.

  2025cites this paper
• The multifaceted roles of non-coding RNAs in cell-cell communication in cardiovascular health and disease

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• The impact of natural and engineered extracellular vesicles on post-myocardial infarction angiogenesis

  2025cites this paper
• ECMSim: A high-performance interactive web application for real-time spatiotemporal simulation of cardiac ECM signaling and diffusion

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• Slit2-robo signaling regulates angiogenesis and repair following myocardial infarction.

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• Gene therapy CM-YAPon protects the mouse heart from myocardial infarction

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• Macrophages in the infarcted heart acquire a fibrogenic phenotype, expressing matricellular proteins, but do not undergo fibroblast conversion.

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• Macrophage‐mediated heart repair and remodeling: A promising therapeutic target for post‐myocardial infarction heart failure

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• Anti‐CTLA‐4 m2a Antibody Exacerbates Cardiac Injury in Experimental Autoimmune Myocarditis Mice By Promoting Ccl5‐Neutrophil Infiltration

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• Analysis of eplerenone in the FDA adverse event reporting system (FAERS) database: a focus on overall patient population and gender-specific subgroups

  2024cites this paper
• Deep skin fibroblast-mediated macrophage recruitment supports acute wound healing

  2024cites this paper
• Regeneration-specific promoter switching facilitates Mest expression in the mouse digit tip to modulate neutrophil response

  2024cites this paper
• Novel Mouse Model for Selective Tagging, Purification, and Manipulation of Cardiac Myofibroblasts.

  2024cites this paper
• Fibroblast Smad7 Induction Protects the Remodeling Pressure-Overloaded Heart

  2024cites this paper
• Repair of the Infarcted Heart: Cellular Effectors, Molecular Mechanisms and Therapeutic Opportunities

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• Cardiac Fibroblastic Niches in Homeostasis and Inflammation

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• Activation of the Macrophage-Associated Inflammasome Exacerbates Myocardial Fibrosis Through the 15-HETE-Mediated Pathway in Acute Myocardial Infarction

  2024cites this paper
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  2024cites this paper
• From Cell to Gene: Deciphering the Mechanism of Heart Failure With Single‐Cell Sequencing

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• Spatial omics of acute myocardial infarction reveals a novel mode of immune cell infiltration

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• Fibroblast Diversity and Epigenetic Regulation in Cardiac Fibrosis

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• Charting the cellular landscape of pulmonary arterial hypertension through single-cell omics

  2024cites this paper
• Persistent transcriptional changes in cardiac adaptive immune cells following myocardial infarction: New evidence from the re-analysis of publicly available single cell and nuclei RNA-sequencing data sets.

  2024cites this paper
• M2 macrophage-derived exosomes promote angiogenesis and improve cardiac function after myocardial infarction

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• Identification of Shared Signature Genes and Immune Microenvironment Subtypes for Heart Failure and Chronic Kidney Disease Based on Machine Learning

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• Single-cell Technology in Stem Cell Research.

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