The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter

No abstract is available for this paper.

• Publication year

  2009

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2009-02-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1073/pnas.0809979106PMID 19179287PMCID PMC2644114
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2008cited by this paper
• Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

  2008influential reference
• Crystal structure of a catalytic intermediate of the maltose transporter

  2007cited by this paper
• Structure of an ABC transporter in complex with its binding protein

  2007cited by this paper
• An Inward-Facing Conformation of a Putative Metal-Chelate-Type ABC Transporter

  2007cited by this paper
• Tripartite ATP-Independent Periplasmic Transporters: Application of a Relational Database for Genome-Wide Analysis of Transporter Gene Frequency and Organization

  2007cited by this paper
• Sialic acid utilization by bacterial pathogens.

  2007cited by this paper
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  2007cited by this paper
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  2006cited by this paper
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  2006cited by this paper
• Conservation of Structure and Mechanism in Primary and Secondary Transporters Exemplified by SiaP, a Sialic Acid Binding Virulence Factor from Haemophilus influenzae*

  2006influential reference
• Novel ligands for the extracellular solute receptors of two bacterial TRAP transporters.

  2006cited by this paper
• Sialic acid transport in Haemophilus influenzae is essential for lipopolysaccharide sialylation and serum resistance and is dependent on a novel tripartite ATP‐independent periplasmic transporter

  2005cited by this paper
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  2005cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
• Lactococcus lactis as host for overproduction of functional membrane proteins.

  2003cited by this paper
• The tripartite tricarboxylate transporter (TTT) family.

  2003cited by this paper
• ABC transporters: one, two or four extracytoplasmic substrate‐binding sites?

  2002cited by this paper
• The tripartite ATP-independent periplasmic (TRAP) transporters of bacteria and archaea.

  2001cited by this paper
• One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

  2000influential reference
• The iscS Gene in Escherichia coli Is Required for the Biosynthesis of 4-Thiouridine, Thiamin, and NAD*

  2000cited by this paper
• Expression of prokaryotic membrane transport proteins in Escherichia coli.

  1999cited by this paper
• TRAP transporters: a new family of periplasmic solute transport systems encoded by the dctPQM genes of Rhodobacter capsulatus and by homologs in diverse gram-negative bacteria

  1997cited by this paper
• Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

  1997cited by this paper
• Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin

  1996cited by this paper
• Glutamate transport in Rhodobacter sphaeroides is mediated by a novel binding protein-dependent secondary transport system.

  1996cited by this paper
• Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

  1995cited by this paper
• Secondary solute transport in bacteria.

  1993cited by this paper
• Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli.

  1991cited by this paper
• MECHANISM OF LACTOSE TRANSLOCATION IN MEMBRANE VESICLES FROM ESCHERICHIA COLI

  1979cited by this paper
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  1979cited by this paper
• A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.

  1978cited by this paper
• Solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine.

  1975cited by this paper

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  2023cites this paper
• Biochemical and structural basis of sialic acid utilization by gut microbes

  2023influential citation
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  2023cites this paper
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  2023cites this paper
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  2023cites this paper
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  2022cites this paper
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  2021cites this paper
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  2020cites this paper
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