The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants.

Microbial patterns are recognized by cell-surface receptors to initiate pattern-triggered immunity (PTI) in plants. Receptor-like cytoplasmic kinases (RLCKs), such as BIK1, and calcium-dependent protein kinases (CPKs) are engaged during PTI to activate the NADPH oxidase RBOHD for reactive oxygen species (ROS) production. It is unknown whether protein kinases besides CPKs and RLCKs participate in RBOHD regulation. We screened mutants in all ten Arabidopsis MAP4 kinases (MAP4Ks) and identified the conserved MAP4K SIK1 as a positive regulator of PTI. sik1 mutants were compromised in their ability to elicit the ROS burst in response to microbial features and exhibited compromised PTI to bacterial infection. SIK1 directly interacts with, phosphorylates, and stabilizes BIK1 in a kinase activity-dependent manner. Furthermore, SIK1 directly interacts with and phosphorylates RBOHD upon flagellin perception. Thus, SIK1 positively regulates immunity by stabilizing BIK1 and activating RBOHD to promote the extracellular ROS burst.

• Publication year

  2018

• Venue

  Cell Host and Microbe

• Publication date

  2018-09-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.chom.2018.08.007PMID 30212650PMCID PMC6279242
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A Regulatory Module Controlling Homeostasis of a Plant Immune Kinase.

  2018cited by this paper
• Ligand-triggered de-repression of Arabidopsis heterotrimeric G proteins coupled to immune receptor kinases

  2018cited by this paper
• The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity.

  2018cited by this paper
• E3 ligase SAUL1 serves as a positive regulator of PAMP-triggered immunity and its homeostasis is monitored by immune receptor SOC3.

  2017cited by this paper
• From Chaos to Harmony: Responses and Signaling upon Microbial Pattern Recognition.

  2017cited by this paper
• Receptor Kinases in Plant-Pathogen Interactions: More Than Pattern Recognition[OPEN]

  2017cited by this paper
• The Hippo/STE20 homolog SIK1 interacts with MOB1 to regulate cell proliferation and cell expansion in Arabidopsis.

  2016influential reference
• Mighty Dwarfs: Arabidopsis Autoimmune Mutants and Their Usages in Genetic Dissection of Plant Immunity

  2016cited by this paper
• Making sense of plant autoimmunity and ‘negative regulators’

  2016cited by this paper
• Plant Aquaporin AtPIP1;4 Links Apoplastic H2O2 Induction to Disease Immunity Pathways1[OPEN]

  2016cited by this paper
• Regulation of pattern recognition receptor signalling in plants

  2016cited by this paper
• Arabidopsis receptor-like cytoplasmic kinase BIK1: purification, crystallization and X-ray diffraction analysis.

  2016cited by this paper
• The Arabidopsis Protein Phosphatase PP2C38 Negatively Regulates the Central Immune Kinase BIK1

  2016cited by this paper
• Arabidopsis heterotrimeric G proteins regulate immunity by directly coupling to the FLS2 receptor

  2016cited by this paper
• The kinase MST4 limits inflammatory responses through direct phosphorylation of the adaptor TRAF6

  2015cited by this paper
• The Pfam protein families database: towards a more sustainable future

  2015cited by this paper
• PBL13 Is a Serine/Threonine Protein Kinase That Negatively Regulates Arabidopsis Immune Responses1[OPEN]

  2015cited by this paper
• Capping protein integrates multiple MAMP signalling pathways to modulate actin dynamics during plant innate immunity

  2015cited by this paper
• Regulation of the NADPH Oxidase RBOHD During Plant Immunity.

  2015influential reference
• The FLS2-associated kinase BIK1 directly phosphorylates the NADPH oxidase RbohD to control plant immunity.

  2014cited by this paper
• Free radicals mediate systemic acquired resistance.

  2014cited by this paper
• The calcium-dependent protein kinase CPK28 buffers plant immunity and regulates BIK1 turnover.

  2014influential reference
• Tyrosine phosphorylation of protein kinase complex BAK1/BIK1 mediates Arabidopsis innate immunity

  2014cited by this paper
• A pipeline for 15N metabolic labeling and phosphoproteome analysis in Arabidopsis thaliana.

  2014cited by this paper
• Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity.

  2014influential reference
• Systemic acquired resistance: turning local infection into global defense.

  2013cited by this paper
• Phosphorylation of BLUS1 kinase by phototropins is a primary step in stomatal opening

  2013cited by this paper
• Phosphorylation of an ERF Transcription Factor by Arabidopsis MPK3/MPK6 Regulates Plant Defense Gene Induction and Fungal Resistance[C][W]

  2013cited by this paper
• Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagation

  2013cited by this paper
• Identification and functional analysis of phosphorylation residues of the Arabidopsis BOTRYTIS-INDUCED KINASE1

  2013cited by this paper
• BSK1, a receptor-like cytoplasmic kinase, involved in both BR signaling and innate immunity in Arabidopsis

  2013cited by this paper
• Inverse modulation of plant immune and brassinosteroid signaling pathways by the receptor-like cytoplasmic kinase BIK1

  2013cited by this paper
• Nonlegumes Respond to Rhizobial Nod Factors by Suppressing the Innate Immune Response

  2013cited by this paper
• Defense-related calcium signaling mutants uncovered via a quantitative high-throughput screen in Arabidopsis thaliana.

  2012cited by this paper
• Quantitative Proteomics Reveals Dynamic Changes in the Plasma Membrane During Arabidopsis Immune Signaling*

  2012cited by this paper
• A Xanthomonas uridine 5′-monophosphate transferase inhibits plant immune kinases

  2012cited by this paper
• T-Coffee: a web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension

  2011cited by this paper
• Phosphorylation-Dependent Differential Regulation of Plant Growth, Cell Death, and Innate Immunity by the Regulatory Receptor-Like Kinase BAK1

  2011cited by this paper
• Biochemical and Genetic Requirements for Function of the Immune Response Regulator BOTRYTIS-INDUCED KINASE1 in Plant Growth, Ethylene Signaling, and PAMP-Triggered Immunity in Arabidopsis[C][W]

  2011cited by this paper
• Protein kinases: evolution of dynamic regulatory proteins.

  2011cited by this paper
• Of PAMPs and Effectors: The Blurred PTI-ETI Dichotomy[OA]

  2011cited by this paper
• Plant Immunity Directly or Indirectly Restricts the Injection of Type III Effectors by the Pseudomonas syringae Type III Secretion System1[W][OA]

  2010cited by this paper
• New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0.

  2010cited by this paper
• Scaffold: A bioinformatic tool for validating MS/MS‐based proteomic studies

  2010cited by this paper
• Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector.

  2010cited by this paper
• RIN4 Functions with Plasma Membrane H+-ATPases to Regulate Stomatal Apertures during Pathogen Attack

  2009cited by this paper
• Enzymatic assembly of DNA molecules up to several hundred kilobases

  2009cited by this paper
• Monitoring plant hormones during stress responses.

  2009cited by this paper
• A receptor-like cytoplasmic kinase, BIK1, associates with a flagellin receptor complex to initiate plant innate immunity

  2009cited by this paper
• DOG 1.0: illustrator of protein domain structures

  2009influential reference
• Phosphorylation of CARMA1 by HPK1 is critical for NF-κB activation in T cells

  2009cited by this paper
• Distinct Roles of Jasmonates and Aldehydes in Plant-Defense Responses

  2008cited by this paper
• Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation.

  2007cited by this paper
• A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence

  2007cited by this paper
• Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses

  2007cited by this paper
• Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis

  2007cited by this paper
• Gateway-compatible vectors for plant functional genomics and proteomics.

  2006cited by this paper
• Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors.

  2005cited by this paper
• The Arabidopsis Receptor Kinase FLS2 Binds flg22 and Determines the Specificity of Flagellin Perception[W]

  2005cited by this paper
• Pathogen-induced, NADPH oxidase–derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana

  2005cited by this paper
• The Membrane-Anchored BOTRYTIS-INDUCED KINASE1 Plays Distinct Roles in Arabidopsis Resistance to Necrotrophic and Biotrophic Pathogens[W]

  2005cited by this paper
• Induction of Protein Secretory Pathway Is Required for Systemic Acquired Resistance

  2005cited by this paper
• Bacterial disease resistance in Arabidopsis through flagellin perception

  2004cited by this paper
• TANDEM: matching proteins with tandem mass spectra.

  2004cited by this paper
• Direct biochemical evidence for type III secretion-dependent translocation of the AvrBs2 effector protein into plant cells

  2002cited by this paper
• Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response

  2001cited by this paper
• The Ste20 group kinases as regulators of MAP kinase cascades.

  2001influential reference
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Characterization of the Pseudomonas syringae pv. tomato AvrRpt2 protein: demonstration of secretion and processing during bacterial pathogenesis

  1999cited by this paper
• Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.

  1998cited by this paper
• Pheromone signalling and polarized morphogenesis in yeast.

  1997cited by this paper
• Requirement of Salicylic Acid for the Induction of Systemic Acquired Resistance

  1993cited by this paper
• Ti plasmid-specified chemotaxis of Agrobacterium tumefaciens C58C1 toward vir-inducing phenolic compounds and soluble factors from monocotyledonous and dicotyledonous plants

  1988cited by this paper
• Bioinformatics Applications Note Gene Expression Skyline: an Open Source Document Editor for Creating and Analyzing Targeted Proteomics Experiments

  year unknowncited by this paper

• Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity

  2026cites this paper
• Overexpression of MdBAK1 Enhances Resistance to Colletotrichum fructicola in Apple (Malus domestica)

  2026cites this paper
• OsMAP4K6 positively regulates salt tolerance via activating OsMKK6 in rice

  2026cites this paper
• Pohsphorylation of MAP4K1 and MAP4K2 by OST1 is required for drought tolerance.

  2026cites this paper
• CBL1/9-CIPK6 complex negatively regulates Respiratory burst oxidase homolog D in Arabidopsis thaliana.

  2026cites this paper
• Phosphorylation and ubiquitination synergistically promote the degradation of OsRbohB to modulate rice immunity.

  2025cites this paper
• CARK3 Interacts with OST1 to Regulate Stomatal Immunity in Arabidopsis.

  2025cites this paper
• Seaweed‐Derived Oligosaccharides Confer Broad‐Spectrum Antiviral Defenses in Tomato and Arabidopsis Plants

  2025cites this paper
• Phosphorylation-dependent activation of MAP4K1/2 by OST1 mediates ABA-induced stomatal closure in Arabidopsis.

  2025cites this paper
• Transcriptional and post-translational regulation of wheat TaWRKY40 orchestrates ROS-mediated plant resistance against stripe rust fungus.

  2025cites this paper
• Receptor‐like cytoplasmic kinase MdPBL34 phosphorylates melatonin biosynthetic enzyme MdSNAT5 to trigger disease resistance to apple Alternaria blotch

  2025cites this paper
• The Ste20 Homologue FvM4K1 Regulates Organ Size via Hippo Signalling Pathway in Woodland Strawberry ( Fragaria vesca )

  2025cites this paper
• Quantitative proteomic and phosphoproteomic analyses reveal salt tolerance mechanisms of the halophyte Hordeum brevisubulatum.

  2025cites this paper
• Pattern Recognition Receptors in Plant Immunity.

  2025cites this paper
• The Aquaporin MdPIP2;2 Regulates Glomerella Leaf Spot Resistance in Apple

  2025cites this paper
• Receptor-like cytoplasmic kinases mediated signaling in plant immunity: convergence and divergence

  2025influential citation
• SAL1‐PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses

  2025cites this paper
• Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato

  2025cites this paper
• Rhizosphere microbes mitigate the shade avoidance responses in Arabidopsis.

  2025cites this paper
• Phosphoproteomic Profiling Deciphers Heat-Stress-Responsive Mechanisms in Passion Fruit

  2025cites this paper
• Flagellin sensing, signaling, and immune responses in plants

  2025influential citation
• The proxiome of a plant viral protein with dual targeting to mitochondria and chloroplasts revealed MAPK cascade and splicing components as proviral factors.

  2025cites this paper
• The pepper MAP4K kinase CaMAP4K3 negatively regulates drought resistance

  2025cites this paper
• Identification of the MAP4K gene family reveals GhMAP4K13 regulates drought and salt stress tolerance in cotton

  2025cites this paper
• Exploring the functional and regulatory mechanisms of the RBOHD gene in rice against bacterial blight disease

  2025cites this paper
• G proteins regulate rice grain size by modulating the Hippo signaling pathway.

  2025cites this paper
• MAP4K1 and MAP4K2 regulate ABA-induced and Ca2+-mediated stomatal closure in Arabidopsis

  2025cites this paper
• NADPH Oxidase Coordinating With ROS Regulation: A Biochemical Footprinting in Plant Development

  2025cites this paper
• Cysteine-rich receptor-like kinases OsCRK19 and OsCRK22 interact with OsRbohI to modulate ROS production and disease resistance in rice.

  2025cites this paper
• The GW5‐WRKY53‐SGW5 module regulates grain size variation in rice

  2024cites this paper
• The Negative Regulators of the Basal Defence WRKY7, WRKY11 and WRKY17 Modulate the Jasmonic Acid Pathway and an Alternative Splicing Regulatory Network in Response to Pseudomonas syringae in Arabidopsis thaliana

  2024influential citation
• Phosphoproteomic analysis uncovers phosphorylated proteins in response to salicylic acid and N-hydroxypipecolic acid in Arabidopsis

  2024cites this paper
• The Multifaceted Ubiquitination of BIK1 During Plant Immunity in Arabidopsis thaliana

  2024cites this paper
• The Phytophthora infestans effector Pi05910 suppresses and destabilizes host glycolate oxidase StGOX4 to promote plant susceptibility

  2024cites this paper
• Shared signals, different fates: Calcium and ROS in plant PRR and NLR immunity.

  2024cites this paper
• Orchestrating ROS regulation: coordinated post-translational modification switches in NADPH oxidases.

  2024cites this paper
• Osmotic signaling releases PP2C-mediated inhibition of Arabidopsis SnRK2s via the receptor-like cytoplasmic kinase BIK1

  2024cites this paper
• Intricate intracellular kinase network regulates the Spodoptera lituta-derived elicitor response signaling in Arabidopsis

  2024influential citation
• Overexpression of GmSRC2 Confers Resistance to Phytophthora sojae in Soybean.

  2024cites this paper
• The MAP4 kinase NbM4K3 regulates immune responses in Nicotiana benthamiana

  2024influential citation
• Gene editing of the E3 ligase PIRE1 fine-tunes ROS production for enhanced bacterial disease resistance in tomato

  2024cites this paper
• The calcium-dependent protein kinase CPK16 regulates hypoxia-induced ROS production by phosphorylating the NADPH oxidase RBOHD in Arabidopsis.

  2024cites this paper
• Catalytically inactive subgroup VIII receptor-like cytoplasmic kinases regulate the immune-triggered oxidative burst in Arabidopsis thaliana

  2024cites this paper
• Receptor-like cytoplasmic kinases: orchestrating plant cellular communication.

  2024cites this paper
• Cell-specialized chloroplast signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses

  2024cites this paper
• Fermentation enrichment, structural characterization and immunostimulatory effects of β-glucan from Quinoa.

  2024cites this paper
• Bioinformatic Identification and Expression Analyses of the MAPK–MAP4K Gene Family Reveal a Putative Functional MAP4K10-MAP3K7/8-MAP2K1/11-MAPK3/6 Cascade in Wheat (Triticum aestivum L.)

  2024cites this paper
• Mitogen-activated protein kinase phosphatase 1 controls broad spectrum disease resistance in Arabidopsis thaliana through diverse mechanisms of immune activation

  2024cites this paper
• Heterodimerization domains in MAP4 KINASEs determine subcellular localization and activity in Arabidopsis.

  2024cites this paper
• S-nitrosylation of a receptor-like cytoplasmic kinase regulates plant immunity

  2024cites this paper
• PTI‐ETI synergistic signal mechanisms in plant immunity

  2024cites this paper
• Unveiling what makes the reactive oxygen species burst transient: the role of PB1CP in plant immunity.

  2024cites this paper
• OsBBX19-OsBTB97/OsBBX11 module regulates spikelet development and yield production in rice.

  2023cites this paper
• Roles and Regulation of the RBOHD Enzyme in Initiating ROS-Mediated Systemic Signaling During Biotic and Abiotic Stress

  2023influential citation
• Sugarcane mosaic virus employs 6K2 protein to impair ScPIP2; 4 transport of H2O2 to facilitate virus infection.

  2023cites this paper
• Identification of Morphogenesis-Related NDR Kinase Signaling Network and Its Regulation on Cold Tolerance in Maize

  2023cites this paper
• The phagocytosis oxidase/Bem1p domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity.

  2023cites this paper
• Enhanced Ca2+ Binding to EF-Hands through Phosphorylation of Conserved Serine Residues Activates MpRBOHB and Chitin-Triggered ROS Production

  2023influential citation
• Research Progress of Immune System in Plants

  2023cites this paper
• Arabidopsis translation initiation factor binding protein CBE1 negatively regulates accumulation of the NADPH oxidase respiratory burst oxidase homolog D

  2023cites this paper
• Phosphorylation of OsRbohB by the CaM-dependent protein kinase OsDMI3 promotes H2O2 production to potentiate ABA responses in rice.

  2023cites this paper
• ETNPPL modulates hyperinsulinemia‐induced insulin resistance through the SIK1/ROS‐mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes

  2023cites this paper
• Wheat yellow mosaic virus NIb targets TaVTC2 to elicit broad‐spectrum pathogen resistance in wheat

  2023cites this paper
• Pseudomonas Cyclic Lipopeptide Medpeptin: Biosynthesis and Modulation of Plant Immunity

  2023cites this paper
• Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling

  2022cites this paper
• Transcriptional Repression of TaNOX10 by TaWRKY19 Compromises ROS Generation and Enhances Wheat Susceptibility to Stripe Rust.

  2022cites this paper
• Correction to: Rice Carbohydrate‑Binding Malectin‑Like Protein, OsCBM1, Contributes to Drought‑Stress Tolerance by Participating in NADPH Oxidase‑Mediated ROS Production

  2022cites this paper
• Arabidopsis CONSERVED BINDING OF EIF4E1 negatively regulates the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D

  2022cites this paper
• Evolutionary and functional characterisation of glutathione peroxidases showed splicing mediated stress responses in Maize.

  2022cites this paper
• Activation and turnover of the plant immune signaling kinase BIK1: a fine balance.

  2022cites this paper
• What’s new in protein kinase/phosphatase signalling in the control of plant immunity?

  2022cites this paper
• MxMPK4-1 phosphorylates NADPH oxidase to trigger the MxMPK6-2-MxbHLH104 pathway mediated Fe deficiency responses in apple.

  2022cites this paper
• Concerted actions of PRR- and NLR-mediated immunity.

  2022cites this paper
• Reactive oxygen species signalling in plant stress responses

  2022cites this paper
• Regulation of plant responses to biotic and abiotic stress by receptor-like cytoplasmic kinases

  2022cites this paper
• Dysfunction of histone demethylase IBM1 in Arabidopsis causes autoimmunity and reshapes the root microbiome

  2022cites this paper
• Sulforaphane, a secondary metabolite in crucifers, inhibits the oxidative stress adaptation and virulence of Xanthomonas by directly targeting OxyR

  2022cites this paper
• Stress-induced ROS compartmentalization, perception, and signaling

  2022cites this paper
• Tomato receptor-like cytosolic kinase RIPK confers broad-spectrum disease resistance without yield penalties

  2022cites this paper
• Phosphorylation of a wheat aquaporin at two sites enhances both plant growth and defense.

  2022cites this paper
• Regulation of PaRBOH1-mediated ROS production in Norway spruce by Ca2+ binding and phosphorylation

  2022cites this paper
• Arabidopsis EXTRA-LARGE G PROTEIN 1 (XLG1) functions together with XLG2 and XLG3 in PAMP-triggered MAPK activation and immunity.

  2022cites this paper
• BPL3 binds the long noncoding RNA nalncFL7 to suppress FORKED-LIKE7 and modulate HAI1-mediated MPK3/6 dephosphorylation in plant immunity.

  2022cites this paper
• The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type‐III effector

  2022cites this paper
• Large-scale analysis of the N-terminal regulatory elements of the kinase domain in plant Receptor-like kinase family

  2022cites this paper
• Glycine maxNNL1 restricts symbiotic compatibility with widely distributed bradyrhizobia via root hair infection

  2021cites this paper
• Potato StMPK7 is a downstream component of StMKK1 and promotes resistance to the oomycete pathogen Phytophthora infestans

  2021cites this paper
• PTI-ETI crosstalk: an integrative view of plant immunity.

  2021cites this paper
• Programming electronic skin with diverse skin-like properties

  2021cites this paper
• An allosteric regulation mechanism of Arabidopsis Serine/Threonine kinase 1 (SIK1) through phosphorylation

  2021cites this paper
• Weighted Gene Co-Expression Analysis Network-Based Analysis on the Candidate Pathways and Hub Genes in Eggplant Bacterial Wilt-Resistance: A Plant Research Study

  2021cites this paper
• Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity.

  2021cites this paper
• Rice Carbohydrate-Binding Malectin-Like Protein, OsCBM1, Contributes to Drought-Stress Tolerance by Participating in NADPH Oxidase-Mediated ROS Production

  2021cites this paper
• Profiling of associated proteins reveals dual functions of the phosphatase ABI1 in abscisic acid signaling

  2021cites this paper
• Structural Elucidation and Immunostimulatory Activities of Quinoa Non-starch Polysaccharide Before and After Deproteinization

  2021cites this paper
• Microbe-Assisted Alleviation of Heavy Metal Toxicity in Plants: A Review

  2021cites this paper
• Isolation And Characterization of Biopolymers From Chenopodium Quinoa And Their Immunostimulatory Activity

  2021cites this paper
• Flg22-induced Ca2+ increases undergo desensitization and resensitization.

  2021cites this paper
• Molecular mechanisms of early plant pattern-triggered immune signaling.

  2021cites this paper
• A Comprehensive Phylogenetic Analysis of the MAP4K Family in the Green Lineage

  2021cites this paper