Efficient Display of Aspergillus niger β-Glucosidase on Saccharomyces cerevisiae Cell Wall for Aroma Enhancement in Wine.

The aim of this study was to evaluate the potential application of cell-surface-displayed β-glucosidase (BGL) in wine aroma enhancement. Gene cassettes for the surface display of Aspergillus niger BGL were constructed using different promoters ( GPD and SED1) and glycosylphosphatidylinositol (GPI) anchoring regions (Sag1, Sed1, and Cwp2). The differences in surface-display cassettes, the tolerance of the displayed BGL to typical winemaking conditions, and the hydrolysis capacity for the liberation of grape aroma glycosides were analyzed. Results revealed that simultaneous utilization of GPD promoter and Sed1 anchoring domain achieved the highest BGL activity. The displayed BGL exhibited relatively high activity at pH 3.0 and at glucose concentration below 2.5% (w/v), compared to commercial enzyme (AR 2000), but exhibited no significant difference under varying ethanol concentrations. Furthermore, the surface-displayed BGL presented better ability to release free terpenols compared to AR 2000. Therefore, a surface-display system could provide a new viable solution for enhancing wine aroma.

• Publication year

  2019

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2019-04-18

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1021/acs.jafc.9b00863PMID 30997795
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The surface-displayed enzyme released more free terpenols from grape aroma glycosides than AR 2000, supporting its use for wine aroma enhancement.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• Varying ethanol concentrations did not produce a significant activity difference for the cell-surface-displayed β-glucosidase compared with AR 2000.

  Confidence 0.90

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• The cell-surface-displayed β-glucosidase retained relatively high activity at pH 3.0 and at glucose concentrations below 2.5% (w/v), outperforming AR 2000 under those conditions.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• Combining the GPD promoter with the Sed1 anchoring domain produced the highest β-glucosidase activity among the tested surface-display cassettes.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review

• ar 2000

  comparative enzyme

  A commercial β-glucosidase preparation used as the comparison enzyme in the activity and aroma-release tests.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• cell-surface-displayed β-glucosidase

  enzyme display system

  A β-glucosidase enzyme engineered to be displayed on the Saccharomyces cerevisiae cell surface.

  Aliases: surface-displayed BGL, displayed BGL

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• free terpenols

  compound class

  Volatile terpenoid alcohols released from bound precursors during hydrolysis and measured as aroma compounds.

  Aliases: terpenols

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• gpd promoter

  promoter

  A constitutive yeast promoter used to drive expression of the β-glucosidase display cassette.

  Aliases: TDH3 promoter

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• grape aroma glycosides

  substrate

  Glycosylated aroma precursors from grapes that can be hydrolyzed to release volatile aroma compounds.

  Aliases: aroma glycosides

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• sed1 anchoring domain

  anchoring domain

  A cell-wall anchoring region from Sed1 used to attach the enzyme display construct to the yeast surface.

  Aliases: Sed1 anchor

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review
• wine aroma enhancement

  application, goal

  The application goal of increasing desirable aroma release in wine by enzymatic hydrolysis of glycosides.

  Aliases: aroma enhancement

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewimjlk (vdp8mqzes2) review

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